{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:17:22Z","timestamp":1784891842328,"version":"3.55.0"},"reference-count":39,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key R&amp;D Program of Hubei Province","award":["2022BBA0057\u201302"],"award-info":[{"award-number":["2022BBA0057\u201302"]}]},{"name":"Key R&amp;D Program of Hubei Province","award":["31772054"],"award-info":[{"award-number":["31772054"]}]},{"name":"Key R&amp;D Program of Hubei Province","award":["NCTIP-XDIC17"],"award-info":[{"award-number":["NCTIP-XDIC17"]}]},{"name":"Key R&amp;D Program of Hubei Province","award":["CQMAITS202312"],"award-info":[{"award-number":["CQMAITS202312"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022BBA0057\u201302"],"award-info":[{"award-number":["2022BBA0057\u201302"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772054"],"award-info":[{"award-number":["31772054"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NCTIP-XDIC17"],"award-info":[{"award-number":["NCTIP-XDIC17"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["CQMAITS202312"],"award-info":[{"award-number":["CQMAITS202312"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Center of Technology Innovation for Pigs","award":["2022BBA0057\u201302"],"award-info":[{"award-number":["2022BBA0057\u201302"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["31772054"],"award-info":[{"award-number":["31772054"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["NCTIP-XDIC17"],"award-info":[{"award-number":["NCTIP-XDIC17"]}]},{"name":"National Center of Technology Innovation for Pigs","award":["CQMAITS202312"],"award-info":[{"award-number":["CQMAITS202312"]}]},{"name":"Chongqing Modern Agricultural Industry Technology System","award":["2022BBA0057\u201302"],"award-info":[{"award-number":["2022BBA0057\u201302"]}]},{"name":"Chongqing Modern Agricultural Industry Technology System","award":["31772054"],"award-info":[{"award-number":["31772054"]}]},{"name":"Chongqing Modern Agricultural Industry Technology System","award":["NCTIP-XDIC17"],"award-info":[{"award-number":["NCTIP-XDIC17"]}]},{"name":"Chongqing Modern Agricultural Industry Technology System","award":["CQMAITS202312"],"award-info":[{"award-number":["CQMAITS202312"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Staphylococcus aureus (S. aureus) is a major foodborne pathogen. Rapid and specific detection is crucial for controlling staphylococcal food poisoning. This study reported a Staphylococcus phage named LSA2302 showing great potential for applications in the rapid detection of S. aureus. Its biological characteristics were identified, including growth properties and stability under different pH and temperature conditions. The genomic analysis revealed that the phage has no genes associated with pathogenicity or drug resistance. Then, the phage-functionalized magnetic beads (pMB), serving as a biological recognition element, were integrated with ATP bioluminescence assays to establish a biosensing method for S. aureus detection. The pMB enrichment brought high specificity and a tenfold increase in analytical sensitivity during detection. The whole detection process could be completed within 30 min, with a broad linear range of 1 \u00d7 104 to 1 \u00d7 108 CFU\/mL and a limit of detection (LOD) of 2.43 \u00d7 103 CFU\/mL. After a 2 h pre-cultivation, this method is capable of detecting bacteria as low as 1 CFU\/mL. The recoveries of S. aureus in spiked skim milk and chicken samples were 81.07% to 99.17% and 86.98% to 104.62%, respectively. Our results indicated that phage-based biosensing can contribute to the detection of target pathogens in foods.<\/jats:p>","DOI":"10.3390\/microorganisms11082098","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T10:42:29Z","timestamp":1692268949000},"page":"2098","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Phage-Based Biosensing for Rapid and Specific Detection of Staphylococcus aureus"],"prefix":"10.3390","volume":"11","author":[{"given":"Ruining","family":"Li","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Joint International Research Laboratory of Animal Health and Animal Food Safety, College of Veterinary Medicine, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5962-2993","authenticated-orcid":false,"given":"Chenxi","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Ding","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/CMR.00134-14","article-title":"Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management","volume":"28","author":"Tong","year":"2015","journal-title":"Clin. 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