{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:29:16Z","timestamp":1760149756177,"version":"build-2065373602"},"reference-count":39,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T00:00:00Z","timestamp":1694131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research and Cultivation Fund of Capital Medical University","award":["PYZ2022135","PX2020065","KJ2023CX049"],"award-info":[{"award-number":["PYZ2022135","PX2020065","KJ2023CX049"]}]},{"name":"Scientific Research and Cultivation Project of Beijing Municipal Hospitals","award":["PYZ2022135","PX2020065","KJ2023CX049"],"award-info":[{"award-number":["PYZ2022135","PX2020065","KJ2023CX049"]}]},{"name":"Beijing Tongzhou District Science and Technology Plan Project","award":["PYZ2022135","PX2020065","KJ2023CX049"],"award-info":[{"award-number":["PYZ2022135","PX2020065","KJ2023CX049"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Tuberculous pleurisy (TP) is one of the most common forms of extrapulmonary tuberculosis, but its diagnosis is challenging. Lipoarabinomannan (LAM) antigen is a biomarker for Mycobacterium tuberculosis (Mtb) infection. LAM detection has potential as an auxiliary diagnostic method for TP. We have successfully generated five rabbit anti-LAM monoclonal antibodies (BJRbL01, BJRbL03, BJRbL20, BJRbL52, and BJRbL76). Here, anti-LAM antibodies were tested to detect LAM in the pleural fluid and plasma of patients with TP by sandwich enzyme-linked immunosorbent assays (ELISAs). The results revealed that all of the anti-LAM antibodies were successfully used as capture and detection antibodies in sandwich ELISAs. The BJRbL01\/BJRbL01-Bio pair showed better performance than the other antibody pairs for detecting mycobacterial clinical isolates and had a limit of detection of 62.5 pg\/mL for purified LAM. LAM levels were significantly higher in the pleural fluid and plasma of patients with TP than in those of patients with malignant pleural effusion or the plasma of non-TB, and LAM levels in the pleural fluid and plasma were positively correlated. Moreover, LAM levels in the pleural fluid sample were significantly higher in confirmed TP patients than in clinically diagnosed TP patients. Our studies provide novel LAM detection choices in the pleural fluid and plasma of TP patients and indicate that LAM detection assay has an auxiliary diagnostic value for TP, which may help to improve the diagnosis of TP.<\/jats:p>","DOI":"10.3390\/microorganisms11092259","type":"journal-article","created":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T09:05:17Z","timestamp":1694423117000},"page":"2259","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunoassay with Novel Paired Antibodies for Detection of Lipoarabinomannan in the Pleural Fluid and Plasma of Patients with Tuberculous Pleurisy"],"prefix":"10.3390","volume":"11","author":[{"given":"Zhuohong","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"},{"name":"Department of Medical Oncology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Immunology, Beijing Key Laboratory on Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panjian","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1020-5080","authenticated-orcid":false,"given":"Hongyun","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongtao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinting","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,8]]},"reference":[{"key":"ref_1","unstructured":"WHO (2022). 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