{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:26:16Z","timestamp":1790087176771,"version":"4.0.1"},"reference-count":93,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NSERC Collaborative Research and Development Grant","award":["532223-18"],"award-info":[{"award-number":["532223-18"]}]},{"name":"NSERC Collaborative Research and Development Grant","award":["2019-01497"],"award-info":[{"award-number":["2019-01497"]}]},{"name":"New Frontiers in Research Fund-Exploration NFRFE","award":["532223-18"],"award-info":[{"award-number":["532223-18"]}]},{"name":"New Frontiers in Research Fund-Exploration NFRFE","award":["2019-01497"],"award-info":[{"award-number":["2019-01497"]}]},{"name":"Faculty of Graduate and Postgraduate Studies at the University of Ottawa","award":["532223-18"],"award-info":[{"award-number":["532223-18"]}]},{"name":"Faculty of Graduate and Postgraduate Studies at the University of Ottawa","award":["2019-01497"],"award-info":[{"award-number":["2019-01497"]}]},{"name":"Nutrition and Mental Health initiative at the Faculty of Health Sciences at the University of Ottawa","award":["532223-18"],"award-info":[{"award-number":["532223-18"]}]},{"name":"Nutrition and Mental Health initiative at the Faculty of Health Sciences at the University of Ottawa","award":["2019-01497"],"award-info":[{"award-number":["2019-01497"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Gut immune system homeostasis is crucial to overall host health. Immune disturbance at the gut level may lead to systemic and distant sites\u2019 immune dysfunction. Probiotics and prebiotics consumption have been shown to improve gut microbiota composition and function and enhance gut immunity. In the current study, the immunomodulatory and anti-inflammatory effects of viable and heat-inactivated forms of the novel probiotic bacterium Rouxiella badensis subsp. acadiensis (Canan SV-53), as well as the prebiotic protocatechuic acid (PCA) derived from the fermentation of blueberry juice by SV-53, were examined. To this end, female Balb\/c mice received probiotic (viable or heat-inactivated), prebiotic, or a mixture of viable probiotic and prebiotic in drinking water for three weeks. To better decipher the immunomodulatory effects of biotics intake, gut microbiota, gut mucosal immunity, T helper-17 (Th17) cell-related cytokines, and epigenetic modulation of Th17 cells were studied. In mice receiving viable SV-53 and PCA, a significant increase was noted in serum IgA levels and the number of IgA-producing B cells in the ileum. A significant reduction was observed in the concentrations of proinflammatory cytokines, including interleukin (IL)-17A, IL-6, and IL-23, and expression of two proinflammatory miRNAs, miR-223 and miR425, in treated groups. In addition, heat-inactivated SV-53 exerted immunomodulatory properties by elevating the IgA concentration in the serum and reducing IL-6 and IL-23 levels in the ileum. DNA methylation analysis revealed the role of heat-inactivated SV-53 in the epigenetic regulation of genes related to Th17 and IL-17 production and function, including Il6, Il17rc, Il9, Il11, Akt1, Ikbkg, Sgk1, Cblb, and Smad4. Taken together, these findings may reflect the potential role of the novel probiotic bacterium SV-53 and prebiotic PCA in improving gut immunity and homeostasis. Further studies are required to ascertain the beneficial effects of this novel bacterium in the inflammatory state.<\/jats:p>","DOI":"10.3390\/microorganisms11102456","type":"journal-article","created":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T16:58:09Z","timestamp":1696179489000},"page":"2456","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Novel Probiotic Bacterium Rouxiella badensis subsp. acadiensis (Canan SV-53) Modulates Gut Immunity through Epigenetic Mechanisms"],"prefix":"10.3390","volume":"11","author":[{"given":"Roghayeh","family":"Shahbazi","sequence":"first","affiliation":[{"name":"Cellular and Molecular Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamed","family":"Yasavoli-Sharahi","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Mallet","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzaneh","family":"Sharifzad","sequence":"additional","affiliation":[{"name":"Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nawal","family":"Alsadi","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrille","family":"Cuenin","sequence":"additional","affiliation":[{"name":"Epigenomics and Mechanisms Branch, International Agency for Research on Cancer (IARC), 25 Av. Tony Garnier, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5530-4368","authenticated-orcid":false,"given":"Vincent","family":"Cahais","sequence":"additional","affiliation":[{"name":"Epigenomics and Mechanisms Branch, International Agency for Research on Cancer (IARC), 25 Av. Tony Garnier, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1043-4948","authenticated-orcid":false,"given":"Felicia Fei-Lei","family":"Chung","sequence":"additional","affiliation":[{"name":"Epigenomics and Mechanisms Branch, International Agency for Research on Cancer (IARC), 25 Av. Tony Garnier, 69007 Lyon, France"},{"name":"Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, Jalan Universiti, Bandar Sunway, Subang Jaya 47500, Selangor, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4109-3154","authenticated-orcid":false,"given":"Zdenko","family":"Herceg","sequence":"additional","affiliation":[{"name":"Epigenomics and Mechanisms Branch, International Agency for Research on Cancer (IARC), 25 Av. Tony Garnier, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Matar","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"},{"name":"School of Nutrition, Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Shahbazi, R., Yasavoli-Sharahi, H., Alsadi, N., Ismail, N., and Matar, C. (2020). Probiotics in Treatment of Viral Respiratory Infections and Neuroinflammatory Disorders. 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