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Among other traits, variations in the composition of gut microbial communities are related to blood lipid patterns and hyperlipidaemia, yet inconsistent association patterns exist. This study aims to assess the relationships between the composition of the gut microbiome and variations in lipid profiles among healthy adults. This study used data and samples from 23 adult participants of a previously conducted dietary intervention study. Circulating lipid measurements and whole-metagenome sequences of the gut microbiome were derived from 180 blood and faecal samples collected from eight visits distributed across an 11-week study. Lipid-related variables explained approximately 4.5% of the variation in gut microbiome compositions, with higher effects observed for total cholesterol and high-density lipoproteins. Species from the genera Odoribacter, Anaerostipes, and Parabacteroides correlated with increased serum lipid levels, whereas probiotic species like Akkermansia muciniphila were more abundant among participants with healthier blood lipid profiles. An inverse correlation with serum cholesterol was also observed for Massilistercora timonensis, a player in regulating lipid turnover. The observed correlation patterns add to the growing evidence supporting the role of the gut microbiome as an essential regulator of host lipid metabolism.<\/jats:p>","DOI":"10.3390\/microorganisms11112656","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T09:27:28Z","timestamp":1698658048000},"page":"2656","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Variations in the Relative Abundance of Gut Bacteria Correlate with Lipid Profiles in Healthy Adults"],"prefix":"10.3390","volume":"11","author":[{"given":"Ineta","family":"Kalnina","sequence":"first","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9446-4521","authenticated-orcid":false,"given":"Dita","family":"Gudra","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivars","family":"Silamikelis","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine","family":"Viksne","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4683-7458","authenticated-orcid":false,"given":"Ance","family":"Roga","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5323-6779","authenticated-orcid":false,"given":"Edmunds","family":"Skinderskis","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davids","family":"Fridmanis","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8362-5505","authenticated-orcid":false,"given":"Janis","family":"Klovins","sequence":"additional","affiliation":[{"name":"Latvian Biomedical Research and Study Centre 1, LV-1067 Riga, Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Puschhof, J., and Elinav, E. (2023). Human Microbiome Research: Growing Pains and Future Promises. PLoS Biol., 21.","DOI":"10.1371\/journal.pbio.3002053"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/s13073-022-01092-0","article-title":"Metabolic Control by the Microbiome","volume":"14","author":"Cox","year":"2022","journal-title":"Genome Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1161\/CIRCRESAHA.118.314642","article-title":"Gut Microbial Associations to Plasma Metabolites Linked to Cardiovascular Phenotypes and Risk: A Cross-Sectional Study","volume":"124","author":"Kurilshikov","year":"2019","journal-title":"Circ. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"811","DOI":"10.2337\/dc21-2358","article-title":"Gut Microbiome Composition Is Predictive of Incident Type 2 Diabetes in a Population Cohort of 5,572 Finnish Adults","volume":"45","author":"Ruuskanen","year":"2022","journal-title":"Diabetes Care"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1038\/s41598-019-38874-3","article-title":"Faecal Bacterial and Short-Chain Fatty Acids Signature in Hypercholesterolemia","volume":"9","author":"Ludwig","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1152\/physiolgenomics.00053.2017","article-title":"Genetic and Microbiome Influence on Lipid Metabolism and Dyslipidemia","volume":"50","author":"Williams","year":"2018","journal-title":"Physiol. Genom."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/nature12506","article-title":"Richness of Human Gut Microbiome Correlates with Metabolic Markers","volume":"500","author":"Nielsen","year":"2013","journal-title":"Nature"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1161\/CIRCRESAHA.115.306807","article-title":"The Gut Microbiome Contributes to a Substantial Proportion of the Variation in Blood Lipids","volume":"117","author":"Fu","year":"2015","journal-title":"Circ. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Miyajima, Y., Karashima, S., Ogai, K., Taniguchi, K., Ogura, K., Kawakami, M., Nambo, H., Kometani, M., Aono, D., and Demura, M. (2022). Impact of Gut Microbiome on Dyslipidemia in Japanese Adults: Assessment of the Shika-Machi Super Preventive Health Examination Results for Causal Inference. Front. Cell Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.908997"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3455","DOI":"10.3748\/wjg.v28.i27.3455","article-title":"Associations of Gut Microbiota with Dyslipidemia Based on Sex Differences in Subjects from Northwestern China","volume":"28","author":"Guo","year":"2022","journal-title":"World J. Gastroenterol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Yun, K.E., Kim, J., Kim, M.H., Park, E., Kim, H.L., Chang, Y., Ryu, S., and Kim, H.N. (2020). Major Lipids, Apolipoproteins, and Alterations of Gut Microbiota. J. Clin. Med., 9.","DOI":"10.3390\/jcm9051589"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"5813","DOI":"10.1038\/s41467-019-13721-1","article-title":"Relationship between Gut Microbiota and Circulating Metabolites in Population-Based Cohorts","volume":"10","author":"Vojinovic","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Castro-Mej\u00eda, J.L., Khakimov, B., Aru, V., Lind, M.V., Garne, E., Paulov\u00e1, P., Tavakkoli, E., Hansen, L.H., Smilde, A.K., and Holm, L. (2022). Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles. Microorganisms, 10.","DOI":"10.3390\/microorganisms10112156"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Rebolledo, C., Cuevas, A., Zambrano, T., Acu\u00f1a, J.J., Jorquera, M.A., Saavedra, K., Mart\u00ednez, C., Lanas, F., Ser\u00f3n, P., and Salazar, L.A. (2017). Bacterial Community Profile of the Gut Microbiota Differs between Hypercholesterolemic Subjects and Controls. Biomed. Res. Int., 2017.","DOI":"10.1155\/2017\/8127814"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Morales, C., Rojas, G., Rebolledo, C., Rojas-Herrera, M., Arias-Carrasco, R., Cuadros-Orellana, S., Maracaja-Coutinho, V., Saavedra, K., Leal, P., and Lanas, F. (2022). Characterization of Microbial Communities from Gut Microbiota of Hypercholesterolemic and Control Subjects. Front. Cell Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.943609"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1080\/00365513.2022.2102540","article-title":"Reduced Gut Microbial Diversity in Familial Hypercholesterolemia with No Effect of Omega-3 Polyunsaturated Fatty Acids Intervention\u2013a Pilot Trial","volume":"82","author":"Hande","year":"2022","journal-title":"Scand. J. Clin. Lab. Invest."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Takagi, T., Naito, Y., Kashiwagi, S., Uchiyama, K., Mizushima, K., Kamada, K., Ishikawa, T., Inoue, R., Okuda, K., and Tsujimoto, Y. (2020). Changes in the Gut Microbiota Are Associated with Hypertension, Hyperlipidemia, and Type 2 Diabetes Mellitus in Japanese Subjects. Nutrients, 12.","DOI":"10.3390\/nu12102996"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.1007\/s00394-018-1821-z","article-title":"Effects of Products Designed to Modulate the Gut Microbiota on Hyperlipidaemia","volume":"58","author":"Deng","year":"2019","journal-title":"Eur. J. Nutr."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1039\/C8FO02163E","article-title":"Effects of Probiotic Supplementation on the Regulation of Blood Lipid Levels in Overweight or Obese Subjects: A Meta-Analysis","volume":"10","author":"Yan","year":"2019","journal-title":"Food Funct."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.numecd.2019.10.001","article-title":"The Impact of Probiotic Yogurt Consumption on Lipid Profiles in Subjects with Mild to Moderate Hypercholesterolemia: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"30","author":"Pourrajab","year":"2020","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_21","first-page":"157","article-title":"The Effect of Probiotics on Serum Lipid Levels in Non-Obese Healthy Adults with Hyperlipidemia: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"39","author":"Sun","year":"2022","journal-title":"Nutr. Hosp."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Yefet, E., Bar, L., Izhaki, I., Iskander, R., Massalha, M., Younis, J.S., and Nachum, Z. (2023). Effects of Probiotics on Glycemic Control and Metabolic Parameters in Gestational Diabetes Mellitus: Systematic Review and Meta-Analysis. Nutrients, 15.","DOI":"10.3390\/nu15071633"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Park, Y.E., Kim, M.S., Shim, K.W., Kim, Y.I., Chu, J., Kim, B.K., Choi, I.S., and Kim, J.Y. (2020). Effects of Lactobacillus Plantarum Q180 on Postprandial Lipid Levels and Intestinal Environment: A Double-Blind, Randomized, Placebo-Controlled, Parallel Trial. Nutrients, 12.","DOI":"10.3390\/nu12010255"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Guan, Z.W., Yu, E.Z., and Feng, Q. (2021). Soluble Dietary Fiber, One of the Most Important Nutrients for the Gut Microbiota. Molecules, 26.","DOI":"10.3390\/molecules26226802"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.advnut.2023.01.005","article-title":"Soluble Fiber Supplementation and Serum Lipid Profile: A Systematic Review and Dose-Response Meta-Analysis of Randomized Controlled Trials","volume":"14","author":"Ghavami","year":"2023","journal-title":"Adv. Nutr."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"109364","DOI":"10.1016\/j.foodres.2020.109364","article-title":"Intake of Soluble Fibre from Chia Seed Reduces Bioaccessibility of Lipids, Cholesterol and Glucose in the Dynamic Gastrointestinal Model Simgi\u00ae","volume":"137","author":"Tamargo","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"787797","DOI":"10.3389\/fimmu.2021.787797","article-title":"The Prebiotic Effects of Oats on Blood Lipids, Gut Microbiota, and Short-Chain Fatty Acids in Mildly Hypercholesterolemic Subjects Compared With Rice: A Randomized, Controlled Trial","volume":"12","author":"Xu","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ojo, O., Ojo, O.O., Zand, N., and Wang, X. (2021). The Effect of Dietary Fibre on Gut Microbiota, Lipid Profile, and Inflammatory Markers in Patients with Type 2 Diabetes: A Systematic Review and Meta-Analysis of Randomised Controlled Trials. Nutrients, 13.","DOI":"10.3390\/nu13061805"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Le Roy, T., L\u00e9cuyer, E., Chassaing, B., Rhimi, M., Lhomme, M., Boudebbouze, S., Ichou, F., Haro Barcel\u00f3, J., Huby, T., and Guerin, M. (2019). The Intestinal Microbiota Regulates Host Cholesterol Homeostasis. BMC Biol., 17.","DOI":"10.1186\/s12915-019-0715-8"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Guo, G., Wu, Y., Liu, Y., Wang, Z., Xu, G., Wang, X., Liang, F., Lai, W., Xiao, X., and Zhu, Q. (2023). Exploring the Causal Effects of the Gut Microbiome on Serum Lipid Levels: A Two-Sample Mendelian Randomization Analysis. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1113334"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.tim.2022.08.003","article-title":"Gut Microbiome-Mediated Mechanisms for Reducing Cholesterol Levels: Implications for Ameliorating Cardiovascular Disease","volume":"31","author":"Jia","year":"2023","journal-title":"Trends Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1194\/jlr.R088989","article-title":"Microbial Impact on Cholesterol and Bile Acid Metabolism: Current Status and Future Prospects","volume":"60","author":"Kriaa","year":"2019","journal-title":"J. Lipid Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1007\/s13679-023-00503-6","article-title":"Short-Chain Fatty Acids\u2014A Product of the Microbiome and Its Participation in Two-Way Communication on the Microbiome-Host Mammal Line","volume":"12","author":"Lange","year":"2023","journal-title":"Curr. Obes. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"10984","DOI":"10.1021\/acs.jafc.7b04666","article-title":"Structure-Specific Effects of Short-Chain Fatty Acids on Plasma Cholesterol Concentration in Male Syrian Hamsters","volume":"65","author":"Zhao","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Vourakis, M., Mayer, G., and Rousseau, G. (2021). The Role of Gut Microbiota on Cholesterol Metabolism in Atherosclerosis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22158074"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Myhrstad, M.C.W., Tunsj\u00f8, H., Charnock, C., and Telle-Hansen, V.H. (2020). Dietary Fiber, Gut Microbiota, and Metabolic Regulation\u2014Current Status in Human Randomized Trials. Nutrients, 12.","DOI":"10.3390\/nu12030859"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Shah, S., Fillier, T., Pham, T.H., Thomas, R., and Cheema, S.K. (2021). Intraperitoneal Administration of Short-Chain Fatty Acids Improves Lipid Metabolism of Long\u2013Evans Rats in a Sex-Specific Manner. Nutrients, 13.","DOI":"10.3390\/nu13030892"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Wang, B., Kong, Q., Li, X., Zhao, J., Zhang, H., Chen, W., and Wang, G. (2020). A High-Fat Diet Increases Gut Microbiota Biodiversity and Energy Expenditure Due to Nutrient Difference. Nutrients, 12.","DOI":"10.3390\/nu12103197"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Zi\u0119tek, M., Celewicz, Z., Kikut, J., and Szczuko, M. (2021). Implications of Scfas on the Parameters of the Lipid and Hepatic Profile in Pregnant Women. Nutrients, 13.","DOI":"10.3390\/nu13061749"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Szczuko, M., Kikut, J., Maciejewska, D., Kulpa, D., Celewicz, Z., and Zi\u0119tek, M. (2020). The Associations of Scfa with Anthropometric Parameters and Carbohydrate Metabolism in Pregnant Women. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21239212"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.chom.2020.05.013","article-title":"Cholesterol Metabolism by Uncultured Human Gut Bacteria Influences Host Cholesterol Level","volume":"28","author":"Kenny","year":"2020","journal-title":"Cell Host Microbe"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1038\/s41564-022-01195-9","article-title":"Characterization of Interactions of Dietary Cholesterol with the Murine and Human Gut Microbiome","volume":"7","author":"Le","year":"2022","journal-title":"Nat. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1053\/j.gastro.2020.09.057","article-title":"The Healthy Microbiome\u2014What Is the Definition of a Healthy Gut Microbiome?","volume":"160","author":"Shanahan","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1093\/eurjpc\/zwad171","article-title":"Causal Relationships between the Gut Microbiome, Blood Lipids, and Heart Failure: A Mendelian Randomization Analysis","volume":"30","author":"Dai","year":"2023","journal-title":"Eur. J. Prev. Cardiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"353","DOI":"10.2188\/jea.JE20170079","article-title":"Genome Database of the Latvian Population (LGDB): Design, Goals, and Primary Results","volume":"28","author":"Rovite","year":"2018","journal-title":"J. Epidemiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/1755-0998.13014","article-title":"A Guide to the Application of Hill Numbers to DNA-Based Diversity Analyses","volume":"19","author":"Alberdi","year":"2019","journal-title":"Mol. Ecol. Resour."},{"key":"ref_47","unstructured":"Kassambara, A., and Mundt, F. (2023, September 05). Factoextra: Extract and Visualize the Results of Multivariate Data Analyses. R Package Version 1.0.7. Available online: https:\/\/CRAN.R-project.org\/package=factoextra."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Hoffman, G.E., and Schadt, E.E. (2016). VariancePartition: Interpreting Drivers of Variation in Complex Gene Expression Studies. BMC Bioinform., 17.","DOI":"10.1186\/s12859-016-1323-z"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Mallick, H., Rahnavard, A., McIver, L.J., Ma, S., Zhang, Y., Nguyen, L.H., Tickle, T.L., Weingart, G., Ren, B., and Schwager, E.H. (2021). Multivariable Association Discovery in Population-Scale Meta-Omics Studies. PLoS Comput. Biol., 17.","DOI":"10.1101\/2021.01.20.427420"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"456","DOI":"10.3389\/fpsyg.2017.00456","article-title":"Repeated Measures Correlation","volume":"8","author":"Bakdash","year":"2017","journal-title":"Front. Psychol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"278","DOI":"10.3389\/fphar.2020.00278","article-title":"Unraveling Host-Gut Microbiota Dialogue and Its Impact on Cholesterol Levels","volume":"11","author":"Villette","year":"2020","journal-title":"Front. Pharmacol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Gargari, G., Deon, V., Taverniti, V., Gardana, C., Denina, M., Riso, P., Guardamagna, O., and Guglielmetti, S. (2018). Evidence of Dysbiosis in the Intestinal Microbial Ecosystem of Children and Adolescents with Primary Hyperlipidemia and the Potential Role of Regular Hazelnut Intake. FEMS Microbiol. Ecol., 94.","DOI":"10.1093\/femsec\/fiy045"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Borrello, K., Lim, U., Park, S.Y., Monroe, K.R., Maskarinec, G., Boushey, C.J., Wilkens, L.R., Randolph, T.W., Le Marchand, L., and Hullar, M.A. (2022). Dietary Intake Mediates Ethnic Differences in Gut Microbial Composition. Nutrients, 14.","DOI":"10.3390\/nu14030660"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mBio.01604-18","article-title":"Impact of Individual Traits, Saturated Fat, and Protein Source on the Gut Microbiome","volume":"9","author":"Lang","year":"2018","journal-title":"mBio"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Bourdeau-Julien, I., Castonguay-Paradis, S., Rochefort, G., Perron, J., Lamarche, B., Flamand, N., Di Marzo, V., Veilleux, A., and Raymond, F. (2023). The Diet Rapidly and Differentially Affects the Gut Microbiota and Host Lipid Mediators in a Healthy Population. Microbiome, 11.","DOI":"10.1186\/s40168-023-01469-2"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Flaig, B., Garza, R., Singh, B., Hamamah, S., and Covasa, M. (2023). Treatment of Dyslipidemia through Targeted Therapy of Gut Microbiota. Nutrients, 15.","DOI":"10.3390\/nu15010228"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s12944-022-01702-1","article-title":"Replacing Saturated Fatty Acids with Polyunsaturated Fatty Acids Increases the Abundance of Lachnospiraceae and Is Associated with Reduced Total Cholesterol Levels\u2014A Randomized Controlled Trial in Healthy Individuals","volume":"21","author":"Gaundal","year":"2022","journal-title":"Lipids Health Dis."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Wang, H., Ma, C., Li, Y., Zhang, L., Yang, C., Zhao, F., Han, H., Shang, D., Yang, F., and Zhang, Y. (2023). Probio-X Relieves Symptoms of Hyperlipidemia by Regulating Patients\u2019 Gut Microbiome, Blood Lipid Metabolism, and Lifestyle Habits. Microbiol. Spectr., 11.","DOI":"10.1128\/spectrum.04440-22"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/mBio.02566-18","article-title":"Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers","volume":"10","author":"Baxter","year":"2019","journal-title":"mBio"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4798","DOI":"10.1038\/s41467-021-25081-w","article-title":"Conversion of Dietary Inositol into Propionate and Acetate by Commensal Anaerostipes Associates with Host Health","volume":"12","author":"Bui","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_61","first-page":"3509","article-title":"Acetate and Butyrate Are the Major Substrates for De Novo Lipogenesis in Rat Colonic Epithelial Cells","volume":"133","author":"Zambell","year":"2003","journal-title":"Nutr. Metab."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"11047","DOI":"10.1038\/s41598-017-10369-z","article-title":"Sulfonolipids as Novel Metabolite Markers of Alistipes and Odoribacter Affected by High-Fat Diets","volume":"7","author":"Walker","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Peterson, C.T., Perez Santiago, J., Iablokov, S.N., Chopra, D., Rodionov, D.A., and Peterson, S.N. (2022). Short-Chain Fatty Acids Modulate Healthy Gut Microbiota Composition and Functional Potential. Curr. Microbiol., 79.","DOI":"10.1007\/s00284-022-02825-5"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Waters, J.L., and Ley, R.E. (2019). The Human Gut Bacteria Christensenellaceae Are Widespread, Heritable, and Associated with Health. BMC Biol., 17.","DOI":"10.1186\/s12915-019-0699-4"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Jian, H., Liu, Y., Wang, X., Dong, X., and Zou, X. (2023). Akkermansia Muciniphila as a Next-Generation Probiotic in Modulating Human Metabolic Homeostasis and Disease Progression: A Role Mediated by Gut\u2013Liver\u2013Brain Axes?. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24043900"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1038\/s41591-019-0495-2","article-title":"Supplementation with Akkermansia Muciniphila in Overweight and Obese Human Volunteers: A Proof-of-Concept Exploratory Study","volume":"25","author":"Depommier","year":"2019","journal-title":"Nat. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1038\/s41564-023-01407-w","article-title":"A genetic system for Akkermansia muciniphila reveals a role for mucin foraging in gut colonization and host sterol biosynthesis gene expression","volume":"8","author":"Davey","year":"2023","journal-title":"Nat. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/ijpo.12262","article-title":"Composition of Gut Microbiota in Obese and Normal-Weight Mexican School-Age Children and Its Association with Metabolic Traits","volume":"13","year":"2018","journal-title":"Pediatr. Obes."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Mazier, W., Le Corf, K., Martinez, C., Tudela, H., Kissi, D., Kropp, C., Coubard, C., Soto, M., Elustondo, F., and Rawadi, G. (2021). A New Strain of Christensenella Minuta as a Potential Biotherapy for Obesity and Associated Metabolic Diseases. Cells, 10.","DOI":"10.3390\/cells10040823"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Zou, Y., Xue, W., Lin, X., Hu, T., Liu, S.W., Sun, C.H., Luo, G., Lv, M., Dai, Y., and Kristiansen, K. (2021). Taxonomic Description and Genome Sequence of Christensenella Intestinihominis Sp. Nov., a Novel Cholesterol-Lowering Bacterium Isolated From Human Gut. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.632361"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"690752","DOI":"10.1155\/2014\/690752","article-title":"Cholesterol-Lowering Potentials of Lactic Acid Bacteria Based on Bile-Salt Hydrolase Activity and Effect of Potent Strains on Cholesterol Metabolism In Vitro and In Vivo","volume":"2014","author":"Tsai","year":"2014","journal-title":"Sci. World J."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Ziarno, M., Zar\u0119ba, D., \u015acibisz, I., and Koz\u0142owska, M. (2023). Exploring the Cholesterol-Modifying Abilities of Lactobacilli Cells in Digestive Models and Dairy Products. Microorganisms, 11.","DOI":"10.20944\/preprints202305.0766.v1"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1053\/j.gastro.2020.03.010","article-title":"Lactococcus Lactis Subspecies Cremoris Elicits Protection Against Metabolic Changes Induced by a Western-Style Diet","volume":"159","author":"Naudin","year":"2020","journal-title":"Gastroenterology"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Martinovi\u0107, A., Cocuzzi, R., Arioli, S., and Mora, D. (2020). Streptococcus Thermophilus: To Survive, or Not to Survive the Gastrointestinal Tract, That Is the Question!. Nutrients, 12.","DOI":"10.3390\/nu12082175"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.bbrc.2021.07.089","article-title":"Safety Assessment of Two Strains and Anti-Obese Effects on Mice Fed a High-Cholesterol Diet","volume":"572","author":"Kuerman","year":"2021","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"5532","DOI":"10.1038\/s41598-021-84928-w","article-title":"Gut Microbiota Markers Associated with Obesity and Overweight in Italian Adults","volume":"11","author":"Palmas","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"11294","DOI":"10.1038\/s41598-023-36533-2","article-title":"Gut-Microbiota in Children and Adolescents with Obesity: Inferred Functional Analysis and Machine-Learning Algorithms to Classify Microorganisms","volume":"13","author":"Squillario","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.celrep.2018.12.028","article-title":"Parabacteroides Distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids","volume":"26","author":"Wang","year":"2019","journal-title":"Cell Rep."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Feng, J., Yang, J., Chang, Y., Qiao, L., Dang, H., Luo, K., Guo, H., An, Y., Ma, C., and Shao, H. (2019). Caffeine-Free Hawk Tea Lowers Cholesterol by Reducing Free Cholesterol Uptake and the Production of Very-Low-Density Lipoprotein. Commun. Biol., 2.","DOI":"10.1038\/s42003-019-0396-4"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Wu, Y., Xu, H., Tu, X., and Gao, Z. (2021). The Role of Short-Chain Fatty Acids of Gut Microbiota Origin in Hypertension. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.730809"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1136\/gutjnl-2018-317609","article-title":"Effects of Dietary Fat on Gut Microbiota and Faecal Metabolites, and Their Relationship with Cardiometabolic Risk Factors: A 6-Month Randomised Controlled-Feeding Trial","volume":"68","author":"Wan","year":"2019","journal-title":"Gut"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"906","DOI":"10.3389\/fimmu.2020.00906","article-title":"The Genus Alistipes: Gut Bacteria With Emerging Implications to Inflammation, Cancer, and Mental Health","volume":"11","author":"Parker","year":"2020","journal-title":"Front. Immunol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"E183","DOI":"10.1017\/S0029665120001317","article-title":"Differences in Gut Microbiota Composition in Metabolic Syndrome and Type 2 Diabetes Subjects in a Multi-Ethnic Population: The HELIUS Study","volume":"79","author":"Deschasaux","year":"2020","journal-title":"Proc. Nutr. Soc."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Torres-S\u00e1nchez, A., Ruiz-Rodr\u00edguez, A., Ortiz, P., and Aguilera, M. (2023). Key Stratification of Microbiota Taxa and Metabolites in the Host Metabolic Health\u2013Disease Balance. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054519"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"100664","DOI":"10.1016\/j.nmni.2020.100664","article-title":"Massilistercora timonensis Gen. Nov., Sp. Nov., a New Bacterium Isolated from the Human Microbiota","volume":"35","author":"Tall","year":"2020","journal-title":"New Microbes New Infect."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Abudujilile, D., Wang, W., Aimaier, A., Chang, L., Dong, Y., Wang, Y., Fan, X., Ma, Y., Wang, Y., and Ziyayiding, D. (2022). Cistanche Tubulosa Phenylethanoid Glycosides Suppressed Adipogenesis in 3T3-L1 Adipocytes and Improved Obesity and Insulin Resistance in High-Fat Diet Induced Obese Mice. BMC Complement. Med. Ther., 22.","DOI":"10.1186\/s12906-022-03743-6"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Hases, L., Stepanauskaite, L., Birgersson, M., Brusselaers, N., Schuppe-Koistinen, I., Archer, A., Engstrand, L., and Williams, C. (2023). High-Fat Diet and Estrogen Modulate the Gut Microbiota in a Sex-Dependent Manner in Mice. Commun. Biol., 6.","DOI":"10.1038\/s42003-022-04406-5"},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Kaakoush, N.O. (2015). Insights into the Role of Erysipelotrichaceae in the Human Host. Front. Cell Infect. Microbiol., 5.","DOI":"10.3389\/fcimb.2015.00084"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Montoya, P., Cerqueda-Garc\u00eda, D., Rodr\u00edguez-Flores, M., L\u00f3pez-Contreras, B., Villamil-Ram\u00edrez, H., Mor\u00e1n-Ramos, S., Molina-Cruz, S., Rivera-Paredez, B., Antuna-Puente, B., and Vel\u00e1zquez-Cruz, R. (2022). Association of Gut Microbiota with Atherogenic Dyslipidemia, and Its Impact on Serum Lipid Levels after Bariatric Surgery. Nutrients, 14.","DOI":"10.3390\/nu14173545"},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Pedersen, K.J., Haange, S.B., \u017d\u00ed\u017ealov\u00e1, K., Viehof, A., Clavel, T., Leni\u010dek, M., Engelmann, B., Wick, L.Y., Schaap, F.G., and Jehmlich, N. (2022). Eggerthella Lenta DSM 2243 Alleviates Bile Acid Stress Response in Clostridium Ramosum and Anaerostipes Caccae by Transformation of Bile Acids. Microorganisms, 10.","DOI":"10.3390\/microorganisms10102025"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1002\/oby.21385","article-title":"Metabolic Effects of Eradicating Breath Methane Using Antibiotics in Prediabetic Subjects with Obesity","volume":"24","author":"Mathur","year":"2016","journal-title":"Obesity"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1038\/s41591-019-0485-4","article-title":"Meta-Omics Analysis of Elite Athletes Identifies a Performance-Enhancing Microbe That Functions via Lactate Metabolism","volume":"25","author":"Scheiman","year":"2019","journal-title":"Nat. Med."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1373\/clinchem.2004.032763","article-title":"Sex and Age Differences in Lipoprotein Subclasses Measured by Nuclear Magnetic Resonance Spectroscopy: The Framingham Study","volume":"50","author":"Freedman","year":"2004","journal-title":"Clin. Chem."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.molmet.2018.05.008","article-title":"Sex Differences in Lipid and Lipoprotein Metabolism","volume":"15","author":"Palmisano","year":"2018","journal-title":"Mol. Metab."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"100912","DOI":"10.1016\/j.yfrne.2021.100912","article-title":"How Biological Sex of the Host Shapes Its Gut Microbiota","volume":"61","author":"Valeri","year":"2021","journal-title":"Front. Neuroendocrinol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.tim.2023.03.010","article-title":"Microbial Endocrinology: The Mechanisms by Which the Microbiota Influences Host Sex Steroids","volume":"31","author":"Cotton","year":"2023","journal-title":"Trends Microbiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"13426","DOI":"10.1038\/s41598-018-31695-w","article-title":"Sex Differences in Lipid Metabolism Are Affected by Presence of the Gut Microbiota","volume":"8","author":"Baars","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Zhu, Q., Qi, N., Shen, L., Lo, C.C., Xu, M., Duan, Q., Ollberding, N.J., Wu, Z., Hui, D.Y., and Tso, P. (2023). Sexual Dimorphism in Lipid Metabolism and Gut Microbiota in Mice Fed a High-Fat Diet. Nutrients, 15.","DOI":"10.3390\/nu15092175"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"e1800870","DOI":"10.1002\/mnfr.201800870","article-title":"Sex Differences in the Gut Microbiota as Potential Determinants of Gender Predisposition to Disease","volume":"63","author":"Haro","year":"2019","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1038\/s41598-017-01576-9","article-title":"Gender Differences in Bile Acids and Microbiota in Relationship with Gender Dissimilarity in Steatosis Induced by Diet and FXR Inactivation","volume":"7","author":"Sheng","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/nature12820","article-title":"Diet Rapidly and Reproducibly Alters the Human Gut Microbiome","volume":"505","author":"David","year":"2014","journal-title":"Nature"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1093\/ajcn\/nqz035","article-title":"Effects of Red Meat, White Meat, and Nonmeat Protein Sources on Atherogenic Lipoprotein Measures in the Context of Low Compared with High Saturated Fat Intake: A Randomized Controlled Trial","volume":"110","author":"Bergeron","year":"2019","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_103","doi-asserted-by":"crossref","unstructured":"Bergwall, S., Johansson, A., Sonestedt, E., and Acosta, S. (2022). High versus Low-Added Sugar Consumption for the Primary Prevention of Cardiovascular Disease. Cochrane Database Syst. Rev.","DOI":"10.1002\/14651858.CD013320.pub2"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1038\/s41564-019-0569-4","article-title":"Cooking Shapes the Structure and Function of the Gut Microbiome","volume":"4","author":"Carmody","year":"2019","journal-title":"Nat. Microbiol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"101828","DOI":"10.1016\/j.bpg.2023.101828","article-title":"The Role of Diet in Shaping Human Gut Microbiota","volume":"62\u201363","author":"Rinninella","year":"2023","journal-title":"Best. Pract. Res. Clin. Gastroenterol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1136\/gutjnl-2019-320438","article-title":"Mediterranean Diet Intervention in Overweight and Obese Subjects Lowers Plasma Cholesterol and Causes Changes in the Gut Microbiome and Metabolome Independently of Energy Intake","volume":"69","author":"Meslier","year":"2020","journal-title":"Gut"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Parolini, C. (2019). Effects of Fish N-3 PUFAs on Intestinal Microbiota and Immune System. Mar. Drugs, 17.","DOI":"10.3390\/md17060374"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1136\/gutjnl-2017-314968","article-title":"A Randomised Trial of the Effect of Omega-3 Polyunsaturated Fatty Acid Supplements on the Human Intestinal Microbiota","volume":"67","author":"Watson","year":"2018","journal-title":"Gut"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"11786388211022378","DOI":"10.1177\/11786388211022378","article-title":"Fish Consumption: A Review of Its Effects on Metabolic and Hormonal Health","volume":"14","author":"Mendivil","year":"2021","journal-title":"Nutr. Metab. Insights"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1093\/advances\/nmab047","article-title":"A Systematic Review of Dietary Influences on Fecal Microbiota Composition and Function among Healthy Humans 1-20 Years of Age","volume":"12","author":"Dinsmoor","year":"2021","journal-title":"Adv. Nutr."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"eaay6218","DOI":"10.1126\/scitranslmed.aay6218","article-title":"Dietary Simple Sugars Alter Microbial Ecology in the Gut and Promote Colitis in Mice","volume":"12","author":"Khan","year":"2020","journal-title":"Sci. Transl. Med."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"e6172","DOI":"10.7717\/peerj.6172","article-title":"Impact of Inter- and Intra-Individual Variation, Sample Storage and Sampling Fraction on Human Stool Microbial Community Profiles","volume":"7","author":"Yeoh","year":"2019","journal-title":"PeerJ"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"850","DOI":"10.3389\/fendo.2019.00850","article-title":"Validity of Dietary Assessment Methods When Compared to the Method of Doubly Labeled Water: A Systematic Review in Adults","volume":"10","author":"Burrows","year":"2019","journal-title":"Front. Endocrinol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.chom.2022.03.002","article-title":"Dynamics of the Normal Gut Microbiota: A Longitudinal One-Year Population Study in Sweden","volume":"30","author":"Olsson","year":"2022","journal-title":"Cell Host Microbe"},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Zhang, F., Zhang, X., Yu, J., Tan, Y., Guo, P., and Wu, C. (2020). The Gut Microbiota Confers the Lipid-Lowering Effect of Bitter Melon (Momordica charantia L.) In High-Fat Diet (HFD)-Induced Hyperlipidemic Mice. Biomed. Pharmacother., 131.","DOI":"10.1016\/j.biopha.2020.110667"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"5995","DOI":"10.1038\/s41467-022-33510-7","article-title":"Whole Genome Sequence Analysis of Blood Lipid Levels in >66,000 Individuals","volume":"13","author":"Selvaraj","year":"2022","journal-title":"Nat. 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