{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T13:35:49Z","timestamp":1767965749010,"version":"3.49.0"},"reference-count":32,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Programa de Est\u00edmulos a la Innovaci\u00f3n","award":["232475"],"award-info":[{"award-number":["232475"]}]},{"name":"Programa de Est\u00edmulos a la Innovaci\u00f3n","award":["273003"],"award-info":[{"award-number":["273003"]}]},{"name":"Programa de Est\u00edmulos a la Innovaci\u00f3n","award":["377414"],"award-info":[{"award-number":["377414"]}]},{"name":"Sistema Nacional de Investigadores (SNI)","award":["232475"],"award-info":[{"award-number":["232475"]}]},{"name":"Sistema Nacional de Investigadores (SNI)","award":["273003"],"award-info":[{"award-number":["273003"]}]},{"name":"Sistema Nacional de Investigadores (SNI)","award":["377414"],"award-info":[{"award-number":["377414"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Human papillomavirus (HPV) is the most prevalent sexually transmitted infection (STI) worldwide, with popular screening methods including the Papanicolaou test and HPV genotyping. However, in clinical practice, coinfections with other pathogens are often underestimated. Therefore, our study aims to describe the prevalence of STIs and vaginosis in urogenital samples from patients who had been tested exclusively for HPV genotyping. Methods: This analytical, prospective, cross-sectional study included 408 males and females. Eligible participants had positive and negative HPV genotyping test results and agreed to early detection or had HPV antecedents. They provided the same urogenital samples used for HPV detection and, through our multiplex in-house PCR assay, we screened for Candida spp., Ureaplasma spp., Trichomonas vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, herpes simplex virus 1 and 2 (HSV), Mycoplasma spp., molluscum contagiosum virus (MCV), Treponema pallidum, Haemophilus spp., Staphylococcus aureus, and Klebsiella spp. The subsequent statistical analysis aimed to reveal correlations between HPV genotypes and the identified pathogens. Results: Of the participants, 72.1% (n = 294) tested positive for HPV genotypes. HR-HPV (high-risk HPV) genotypes comprised 51 (8.1%), 66 (7.1%), and 58 (6.1%). Haemophilus spp., Ureaplasma spp., Candida spp., Staphylococcus aureus, and Mycoplasma spp. frequently co-occurred with HPV infection (p &lt; 0.05). Gender-based variations were notorious for Ureaplasma spp., Mycoplasma spp., and MCV (p &lt; 0.05). Coinfections were prevalent (43.9%), with a positive HPV result elevating the risk for Trichomonas vaginalis, Mycoplasma spp., Staphylococcus aureus, HSV, and MCV (OR &gt; 1, p &lt; 0.05). HPV 16 correlated with HSV and Ureaplasma spp., while HPV 6 was linked with HSV and MCV (p &lt; 0.05). Conclusions: This screening strategy uncovered significant coinfections and associations between HPV genotypes and pathogens, underscoring the importance of routine screening to explore clinical implications in urogenital health.<\/jats:p>","DOI":"10.3390\/microorganisms11112661","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T12:50:42Z","timestamp":1698670242000},"page":"2661","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unveiling Hidden Risks: Intentional Molecular Screening for Sexually Transmitted Infections and Vaginosis Pathogens in Patients Who Have Been Exclusively Tested for Human Papillomavirus Genotyping"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2287-7041","authenticated-orcid":false,"given":"Fabiola","family":"Hern\u00e1ndez-Rosas","sequence":"first","affiliation":[{"name":"Biomedical Engineering Faculty, Engineering Division, Anahuac Queretaro University, Quer\u00e9taro 76246, Mexico"},{"name":"Research Center, Anahuac Queretaro University, Quer\u00e9taro 76246, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Rey-Barrera","sequence":"additional","affiliation":[{"name":"Cambrico Biotech, 41015 Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavio","family":"Hern\u00e1ndez-Barajas","sequence":"additional","affiliation":[{"name":"Translational Medicine, Vanguard and Technology Transfer Sector, Human Health Department, Central ADN Laboratories, Morelia 58280, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Rangel-Soto","sequence":"additional","affiliation":[{"name":"Translational Medicine, Vanguard and Technology Transfer Sector, Human Health Department, Central ADN Laboratories, Morelia 58280, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Socorro","family":"Garc\u00eda-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Escuela de Medicina, Universidad Vasco de Quiroga, Morelia 58090, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shumeyker Susmith","family":"Franco-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Escuela de Medicina, Universidad Vasco de Quiroga, Morelia 58090, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mercedes Piedad","family":"de Le\u00f3n-Bautista","sequence":"additional","affiliation":[{"name":"Translational Medicine, Vanguard and Technology Transfer Sector, Human Health Department, Central ADN Laboratories, Morelia 58280, Mexico"},{"name":"Escuela de Medicina, Universidad Vasco de Quiroga, Morelia 58090, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Martinelli, M., Musumeci, R., Rizzo, A., Muresu, N., Piana, A., Sotgiu, G., Landoni, F., and Cocuzza, C. 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