{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:53:34Z","timestamp":1782820414976,"version":"3.54.5"},"reference-count":98,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T00:00:00Z","timestamp":1699056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National University Jorge Basadre Grohmann","award":["4723-2015-UN\/JBG"],"award-info":[{"award-number":["4723-2015-UN\/JBG"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Lyophilization is a widely employed long-term preservation method in which the bacterial survival rate largely depends on the cryoprotectant used. Bacillus cereus strain PBC was selected for its ability to thrive in environments contaminated with arsenic, lead, and cadmium, tolerate 500 ppm of free cyanide, and the presence of genes such as ars, cad, ppa, dap, among others, associated with the bioremediation of toxic compounds and enterotoxins (nheA, nheB, nheC). Following lyophilization, the survival rates for Mannitol 2.5%, Mannitol 10%, and Glucose 1% were 98.02%, 97.12%, and 96.30%, respectively, with the rates being lower than 95% for other sugars. However, during storage, for the same sugars, the survival rates were 78.71%, 97.12%, and 99.97%, respectively. In the cake morphology, it was found that the lyophilized morphology showed no relationship with bacterial survival rate. The best cryoprotectant for the PBC strain was 1% glucose since it maintained constant and elevated bacterial growth rates during storage, ensuring that the unique characteristics of the bacterium were preserved over time. These findings hold significant implications for research as they report a new Bacillus cereus strain with the potential to be utilized in bioremediation processes.<\/jats:p>","DOI":"10.3390\/microorganisms11112705","type":"journal-article","created":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T14:51:01Z","timestamp":1699109461000},"page":"2705","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of Lyoprotective Agents on the Preservation of Survival of a Bacillus cereus Strain PBG in the Freeze-Drying Process"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7644-9774","authenticated-orcid":false,"given":"Diana Galeska","family":"Farfan Pajuelo","sequence":"first","affiliation":[{"name":"Bioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Carpio Mamani","sequence":"additional","affiliation":[{"name":"Bioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0034-2276","authenticated-orcid":false,"given":"Gisela July","family":"Maraza Choque","sequence":"additional","affiliation":[{"name":"Bioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina Mayumi","family":"Chachaque Callo","sequence":"additional","affiliation":[{"name":"Bioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8822-5954","authenticated-orcid":false,"given":"C\u00e9sar Julio","family":"C\u00e1ceda Quiroz","sequence":"additional","affiliation":[{"name":"Bioremediation Laboratory, Jorge Basadre Grohmann National University, Tacna 230001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/978-3-7643-8340-4_6","article-title":"Heavy Metal Toxicity and the Environment","volume":"Volume 101","author":"Luch","year":"2012","journal-title":"Molecular, Clinical and Environmental Toxicology"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ojuederie, O., and Babalola, O. 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