{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:11:04Z","timestamp":1790745064517,"version":"4.1.0"},"reference-count":80,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T00:00:00Z","timestamp":1699315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Unexpected atypical isolates of Bacillus cereus s.l. occasionally challenge conventional microbiology and even the most advanced techniques for anthrax detection. For anticipating and gaining trust, 65 isolates of Bacillus cereus s.l. of diverse origin were sequenced and characterized. The BTyper3 tool was used for assignation to genomospecies B. mosaicus (34), B. cereus s.s (29) and B. toyonensis (2), as well as virulence factors and toxin profiling. None of them carried any capsule or anthrax-toxin genes. All harbored the non-hemolytic toxin nheABC and sphygomyelinase spH genes, whereas 41 (63%), 30 (46%), 11 (17%) and 6 (9%) isolates harbored cytK-2, hblABCD, cesABCD and at least one insecticidal toxin gene, respectively. Matrix-assisted laser desorption ionization-time of flight mass spectrometry confirmed the production of cereulide (ces genes). Phylogeny inferred from single-nucleotide polymorphisms positioned isolates relative to the B. anthracis lineage. One isolate (BC38B) was of particular interest as it appeared to be the closest B. anthracis neighbor described so far. It harbored a large plasmid similar to other previously described B. cereus s.l. megaplasmids and at a lower extent to pXO1. Whereas bacterial collection is enriched, these high-quality public genetic data offer additional knowledge for better risk assessment using future NGS-based technologies of detection.<\/jats:p>","DOI":"10.3390\/microorganisms11112721","type":"journal-article","created":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T11:23:39Z","timestamp":1699356219000},"page":"2721","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Get to Know Your Neighbors: Characterization of Close Bacillus anthracis Isolates and Toxin Profile Diversity in the Bacillus cereus Group"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1606-1778","authenticated-orcid":false,"given":"Mehdi","family":"Abdelli","sequence":"first","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, 91710 Vert-le-Petit, France"},{"name":"Institute for Integrative Biology of the Cell (I2BC), CNRS, Universit\u00e9 Paris-Saclay, 91190 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Falaise","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, 91710 Vert-le-Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Morineaux-Hilaire","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, 91710 Vert-le-Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"Cumont","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, 91710 Vert-le-Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Taysse","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, 91710 Vert-le-Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Raynaud","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, 91710 Vert-le-Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6576-5387","authenticated-orcid":false,"given":"Vincent","family":"Ramisse","sequence":"additional","affiliation":[{"name":"DGA CBRN Defence Center, Biology Division, French Ministry of the Armed Forces, 91710 Vert-le-Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,7]]},"reference":[{"key":"ref_1","unstructured":"(2023, June 29). 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