{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:45:11Z","timestamp":1791917111702,"version":"4.3.4"},"reference-count":79,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T00:00:00Z","timestamp":1699660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"internal grants of the BfR","award":["45-009"],"award-info":[{"award-number":["45-009"]}]},{"name":"internal grants of the BfR","award":["1322-714"],"award-info":[{"award-number":["1322-714"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Non-O1 and non-O139 Vibrio cholerae (NOVC) can cause gastrointestinal infections in humans. Contaminated food, especially seafood, is an important source of human infections. In this study, the virulence potential of 63 NOVC strains isolated from retail seafood were characterized at the genotypic and phenotypic levels. Although no strain encoded the cholera toxin (CTX) and the toxin-coregulated pilus (TCP), several virulence factors, including the HlyA hemolysin, the cholix toxin ChxA, the heat-stable enterotoxin Stn, and genes coding for the type 3 and type 6 secretion systems, were detected. All strains showed hemolytic activity against human and sheep erythrocytes: 90% (n = 57) formed a strong biofilm, 52% (n = 33) were highly motile at 37 \u00b0C, and only 8% (n = 5) and 14% (n = 9) could resist \u226560% and \u226540% human serum, respectively. Biofilm formation and toxin regulation genes were also detected. cgMLST analysis demonstrated that NOVC strains from seafood cluster with clinical NOVC strains. Antimicrobial susceptibility testing (AST) results in the identification of five strains that developed non-wildtype phenotypes (medium and resistant) against the substances of the classes of beta-lactams (including penicillin, carbapenem, and cephalosporin), polymyxins, and sulphonamides. The phenotypic resistance pattern could be partially attributed to the acquired resistance determinants identified via in silico analysis. Our results showed differences in the virulence potential of the analyzed NOVC isolated from retail seafood products, which may be considered for further pathogenicity evaluation and the risk assessment of NOVC isolates in future seafood monitoring.<\/jats:p>","DOI":"10.3390\/microorganisms11112751","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T02:10:43Z","timestamp":1699841443000},"page":"2751","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Genetic and Phenotypic Virulence Potential of Non-O1\/Non-O139 Vibrio cholerae Isolated from German Retail Seafood"],"prefix":"10.3390","volume":"11","author":[{"given":"Quantao","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Food Safety and Food Hygiene, School of Veterinary Medicine, Freie Universit\u00e4t Berlin, K\u00f6nigsweg 69, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Alter","sequence":"additional","affiliation":[{"name":"Institute of Food Safety and Food Hygiene, School of Veterinary Medicine, Freie Universit\u00e4t Berlin, K\u00f6nigsweg 69, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2494-0278","authenticated-orcid":false,"given":"Eckhard","family":"Strauch","sequence":"additional","affiliation":[{"name":"Department Biological Safety, German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6930-4358","authenticated-orcid":false,"given":"Jens Andre","family":"Hammerl","sequence":"additional","affiliation":[{"name":"Department Biological Safety, German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keike","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department Biological Safety, German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1655-0456","authenticated-orcid":false,"given":"Maria","family":"Borowiak","sequence":"additional","affiliation":[{"name":"Department Biological Safety, German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2509-4950","authenticated-orcid":false,"given":"Carlus","family":"Deneke","sequence":"additional","affiliation":[{"name":"Department Biological Safety, German Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Fleischmann","sequence":"additional","affiliation":[{"name":"Institute of Food Safety and Food Hygiene, School of Veterinary Medicine, Freie Universit\u00e4t Berlin, K\u00f6nigsweg 69, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1890\/1540-9295(2003)001[0080:EMAHPT]2.0.CO;2","article-title":"Environmental microbe and human pathogen: The ecology and microbiology of Vibrio cholerae","volume":"1","author":"Cottingham","year":"2003","journal-title":"Front. 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