{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:34:03Z","timestamp":1792805643352,"version":"4.5.0"},"reference-count":101,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:00:00Z","timestamp":1699833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fogarty International Center","award":["D43 TW010331"],"award-info":[{"award-number":["D43 TW010331"]}],"id":[{"id":"https:\/\/ror.org\/02xey9a22","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Fogarty International Center","award":["111584467514"],"award-info":[{"award-number":["111584467514"]}],"id":[{"id":"https:\/\/ror.org\/02xey9a22","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000061","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["D43 TW010331"],"award-info":[{"award-number":["D43 TW010331"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000061","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["111584467514"],"award-info":[{"award-number":["111584467514"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Minciencias (Colombia)","award":["D43 TW010331"],"award-info":[{"award-number":["D43 TW010331"]}]},{"name":"Minciencias (Colombia)","award":["111584467514"],"award-info":[{"award-number":["111584467514"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Leptospirosis represents a public health problem in Colombia. However, the underreporting of the disease is an unfortunate reality, with a clear trend towards a decrease in cases since 2019, when the guidelines for its confirmatory diagnosis changed with the requirement of two paired samples. The purpose of this review is to highlight the importance of leptospirosis. While the access to rapid diagnosis is available at practically all levels of care for dengue and malaria, leptospirosis\u2014a doubly neglected disease\u2014deserves recognition as a serious public health problem in Colombia. In this manner, it is proposed that molecular tests are a viable diagnostic alternative that can improve the targeted treatment of the patient and the timeliness of data and case reporting to SIVIGILA, and reduce the underreporting of the disease. Taking advantage of the strengthened technological infrastructure derived from the SARS-CoV-2 pandemic for molecular diagnosis in Colombia, with a network of 227 laboratories distributed throughout the national territory, with an installed capacity for PCR testing, it is proposed that molecular diagnosis can be used as an alternative for early diagnosis. This would allow case confirmation through the public health network in Colombia, and, together with the microagglutination (MAT) technique, the epidemiological surveillance of this disease in this country would be strengthened.<\/jats:p>","DOI":"10.3390\/microorganisms11112759","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T08:35:52Z","timestamp":1699864552000},"page":"2759","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Molecular Diagnosis as an Alternative for Public Health Surveillance of Leptospirosis in Colombia"],"prefix":"10.3390","volume":"11","author":[{"given":"Margarita","family":"Arboleda","sequence":"first","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7831-9930","authenticated-orcid":false,"given":"Mariana","family":"Mej\u00eda-Torres","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maritza","family":"Posada","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicaela","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"R\u00edos-Tapias","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Alberto","family":"Rivera-Pedroza","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Calle","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miryan M.","family":"S\u00e1nchez-Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerine","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Tropical Medicine Group, Colombian Institute of Tropical Medicine, Sabaneta 055450, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0002-776X","authenticated-orcid":false,"given":"Piedad","family":"Agudelo-Fl\u00f3rez","sequence":"additional","affiliation":[{"name":"Graduate School, Universidad CES, Medell\u00edn 050021, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,13]]},"reference":[{"key":"ref_1","first-page":"e23","article-title":"Pathology and Pathogenesis of Human Leptospirosis: A Commented Review","volume":"60","author":"Abreu","year":"2018","journal-title":"Rev. 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