{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:56:29Z","timestamp":1787028989846,"version":"build-2736575974"},"reference-count":58,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"French National Agency for Research Grants","award":["ANR-19-CE02-0005-01"],"award-info":[{"award-number":["ANR-19-CE02-0005-01"]}]},{"name":"Lesaffre International","award":["ANR-19-CE02-0005-01"],"award-info":[{"award-number":["ANR-19-CE02-0005-01"]}]},{"name":"Science Foundation Ireland","award":["ANR-19-CE02-0005-01"],"award-info":[{"award-number":["ANR-19-CE02-0005-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The methanogenic strain Mx-05T was isolated from the human fecal microbiome. A phylogenetic analysis based on the 16S rRNA gene and protein marker genes indicated that the strain is affiliated with the order Methanomassiliicoccales. It shares 86.9% 16S rRNA gene sequence identity with Methanomassiliicoccus luminyensis, the only member of this order previously isolated. The cells of Mx-05T were non-motile cocci, with a diameter range of 0.4\u20130.7 \u03bcm. They grew anaerobically and reduced methanol, monomethylamine, dimethylamine, and trimethylamine into methane, using H2 as an electron donor. H2\/CO2, formate, ethanol, and acetate were not used as energy sources. The growth of Mx-05T required an unknown medium factor(s) provided by Eggerthella lenta and present in rumen fluid. Mx-05T grew between 30 \u00b0C and 40 \u00b0C (optimum 37 \u00b0C), over a pH range of 6.9\u20138.3 (optimum pH 7.5), and between 0.02 and 0.34 mol.L\u22121 NaCl (optimum 0.12 mol.L\u22121 NaCl). The genome is 1.67 Mbp with a G+C content of 55.5 mol%. Genome sequence annotation confirmed the absence of the methyl branch of the H4MPT Wood\u2013Ljungdahl pathway, as described for other Methanomassiliicoccales members. Based on an average nucleotide identity analysis, we propose strain Mx-05T as being a novel representative of the order Methanomassiliicoccales, within the novel family Methanomethylophilaceae, for which the name Methanomethylophilus alvi gen. nov, sp. nov. is proposed. The type strain is Mx-05T (JCM 31474T).<\/jats:p>","DOI":"10.3390\/microorganisms11112794","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T09:10:38Z","timestamp":1700212238000},"page":"2794","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov."],"prefix":"10.3390","volume":"11","author":[{"given":"Guillaume","family":"Borrel","sequence":"first","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Evolutionary Biology of the Microbial Cell, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9166-5857","authenticated-orcid":false,"given":"Khaled","family":"Fadhlaoui","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ., Universit\u00e9 de Toulon, CNRS, IRD, MIO, 13288 Marseille, France"},{"name":"Universit\u00e9 Clermont Auvergne, INRA, MEDIS, 63000 Clermont-Ferrand, France"},{"name":"Universit\u00e9 Clermont Auvergne, CNRS, UMR 6023 CNRS-UCA, Laboratoire Microorganismes: G\u00e9nome et Environnement LMGE, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wajdi","family":"Ben Hania","sequence":"additional","affiliation":[{"name":"Aix Marseille Univ., Universit\u00e9 de Toulon, CNRS, IRD, MIO, 13288 Marseille, France"},{"name":"Universit\u00e9 d\u2019Auvergne, EA CIDAM, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Gaci","sequence":"additional","affiliation":[{"name":"Universit\u00e9 d\u2019Auvergne, EA CIDAM, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6479-9470","authenticated-orcid":false,"given":"G\u00e9rard","family":"Pehau-Arnaudet","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Ultrastructural Bioimaging, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3467-8608","authenticated-orcid":false,"given":"Prem Prashant","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Universit\u00e9 d\u2019Auvergne, EA CIDAM, 63000 Clermont-Ferrand, France"},{"name":"Epithelial Therapeutics Unit, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Vandekerckove","sequence":"additional","affiliation":[{"name":"Lesaffre International, Lesaffre Group, 59700 Marcq-en-Bar\u0153ul, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6474-7678","authenticated-orcid":false,"given":"Nathalie","family":"Ballet","sequence":"additional","affiliation":[{"name":"Lesaffre International, Lesaffre Group, 59700 Marcq-en-Bar\u0153ul, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Alric","sequence":"additional","affiliation":[{"name":"Universit\u00e9 d\u2019Auvergne, EA CIDAM, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul William","family":"O\u2019Toole","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland, University College Cork, T12 K8AF Cork, 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