{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:13:39Z","timestamp":1790730819823,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["21-14-00034"],"award-info":[{"award-number":["21-14-00034"]}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-14-00034"],"award-info":[{"award-number":["21-14-00034"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The scavenging of atmospheric trace gases has been recognized as one of the lifestyle-defining capabilities of microorganisms in terrestrial polar ecosystems. Several metagenome-assembled genomes of as-yet-uncultivated methanotrophic bacteria, which consume atmospheric CH4 in these ecosystems, have been retrieved in cultivation-independent studies. In this study, we isolated and characterized a representative of these methanotrophs, strain D3K7, from a subarctic soil of northern Russia. Strain D3K7 grows on methane and methanol in a wide range of temperatures, between 5 and 30 \u00b0C. Weak growth was also observed on acetate. The presence of acetate in the culture medium stimulated growth at low CH4 concentrations (~100 p.p.m.v.). The finished genome sequence of strain D3K7 is 4.15 Mb in size and contains about 3700 protein-encoding genes. According to the result of phylogenomic analysis, this bacterium forms a common clade with metagenome-assembled genomes obtained from the active layer of a permafrost thaw gradient in Stordalen Mire, Abisco, Sweden, and the mineral cryosol at Axel Heiberg Island in the Canadian High Arctic. This clade occupies a phylogenetic position in between characterized Methylocapsa methanotrophs and representatives of the as-yet-uncultivated upland soil cluster alpha (USC\u03b1). As shown by the global distribution analysis, D3K7-like methanotrophs are not restricted to polar habitats but inhabit peatlands and soils of various climatic zones.<\/jats:p>","DOI":"10.3390\/microorganisms11112800","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T10:10:06Z","timestamp":1700215806000},"page":"2800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["One Step Closer to Enigmatic USC\u03b1 Methanotrophs: Isolation of a Methylocapsa-like Bacterium from a Subarctic Soil"],"prefix":"10.3390","volume":"11","author":[{"given":"Olga V.","family":"Danilova","sequence":"first","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave. 33\/2, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor Y.","family":"Oshkin","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave. 33\/2, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4155-5558","authenticated-orcid":false,"given":"Svetlana E.","family":"Belova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave. 33\/2, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirill K.","family":"Miroshnikov","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave. 33\/2, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia A.","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave. 33\/2, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8903-8053","authenticated-orcid":false,"given":"Svetlana N.","family":"Dedysh","sequence":"additional","affiliation":[{"name":"Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave. 33\/2, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,17]]},"reference":[{"key":"ref_1","first-page":"2058","article-title":"Global atmospheric methane: Budget, changes and dangers","volume":"369","author":"Dlugokencky","year":"2011","journal-title":"Philos. 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