{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:14:55Z","timestamp":1789010095954,"version":"build-2803163510"},"reference-count":86,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002848","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","award":["FONDECYT 1221193"],"award-info":[{"award-number":["FONDECYT 1221193"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Millenium Institute Center for Genome Regulation","award":["FONDECYT 1221193"],"award-info":[{"award-number":["FONDECYT 1221193"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Salar de Ascot\u00e1n is a high-altitude arsenic-rich salt flat exposed to high ultraviolet radiation in the Atacama Desert, Chile. It hosts unique endemic flora and fauna and is an essential habitat for migratory birds, making it an important site for conservation and protection. However, there is limited information on the resident microbiota\u2019s diversity, genomic features, metabolic potential, and molecular mechanisms that enable it to thrive in this extreme environment. We used long- and short-read metagenomics to investigate the microbial communities in Ascot\u00e1n\u2019s water, sediment, and soil. Bacteria predominated, mainly Pseudomonadota, Acidobacteriota, and Bacteroidota, with a remarkable diversity of archaea in the soil. Following hybrid assembly, we recovered high-quality bacterial (101) and archaeal (6) metagenome-assembled genomes (MAGs), including representatives of two putative novel families of Patescibacteria and Pseudomonadota and two novel orders from the archaeal classes Halobacteriota and Thermoplasmata. We found different metabolic capabilities across distinct lineages and a widespread presence of genes related to stress response, DNA repair, and resistance to arsenic and other metals. These results highlight the remarkable diversity and taxonomic novelty of the Salar de Ascot\u00e1n microbiota and its rich functional repertoire, making it able to resist different harsh conditions. The highly complete MAGs described here could serve future studies and bioprospection efforts focused on salt flat extremophiles, and contribute to enriching databases with microbial genome data from underrepresented regions of our planet.<\/jats:p>","DOI":"10.3390\/microorganisms11112819","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T11:31:36Z","timestamp":1700479896000},"page":"2819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Diversity, Taxonomic Novelty, and Encoded Functions of Salar de Ascot\u00e1n Microbiota, as Revealed by Metagenome-Assembled Genomes"],"prefix":"10.3390","volume":"11","author":[{"given":"Marcelo","family":"Veloso","sequence":"first","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angie","family":"Waldisperg","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3839-4039","authenticated-orcid":false,"given":"Patricio","family":"Arros","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7780-0856","authenticated-orcid":false,"given":"Camilo","family":"Berr\u00edos-Past\u00e9n","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn","family":"Acosta","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazajem","family":"Colque","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Macarena A.","family":"Varas","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"},{"name":"Millenium Institute Center for Genome Regulation, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2783-2152","authenticated-orcid":false,"given":"Miguel L.","family":"Allende","sequence":"additional","affiliation":[{"name":"Millenium Institute Center for Genome Regulation, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis H.","family":"Orellana","sequence":"additional","affiliation":[{"name":"Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Celsiusstr. 1, D-28359 Bremen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2950-7510","authenticated-orcid":false,"given":"Andr\u00e9s E.","family":"Marcoleta","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Integrativa, Laboratorio de Biolog\u00eda Estructural y Molecular BEM, Faculty of Science, Universidad de Chile, Las Palmeras 3425, \u00d1u\u00f1oa, Santiago 7800003, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,20]]},"reference":[{"key":"ref_1","first-page":"37","article-title":"Elementos Del Clima En El Altiplano Sudamericano","volume":"44","author":"Aceituno","year":"1996","journal-title":"Rev. Geofis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1029\/2008JG000818","article-title":"The High-Lakes Project","volume":"114","author":"Cabrol","year":"2009","journal-title":"J. Geophys. Res. Biogeosci."},{"key":"ref_3","unstructured":"Risacher, F., Alonso, H., and Salazar, C. (2023, September 01). Geoqu\u00edmica de Aguas En Cuencas Cerradas: I, II y III Regiones-Chile. Volumen III ESTUDIO DE CUENCAS DE LA II REGION. Available online: https:\/\/bibliotecadigital.ciren.cl\/handle\/20.500.13082\/32750."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s00792-012-0452-1","article-title":"Enrichment of Arsenic Transforming and Resistant Heterotrophic Bacteria from Sediments of Two Salt Lakes in Northern Chile","volume":"16","author":"Lara","year":"2012","journal-title":"Extremophiles"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Escudero, L.V., Casamayor, E.O., Chong, G., Pedr\u00f3s-Ali\u00f3, C., and Demergasso, C. (2013). Distribution of Microbial Arsenic Reduction, Oxidation and Extrusion Genes along a Wide Range of Environmental Arsenic Concentrations. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0078890"},{"key":"ref_6","unstructured":"Dorador, C. (2007). Microbial Communities in High Altitude Altiplanic Wetlands in Northern Chile: Phylogeny, Diversity and Function. [Ph.D. Thesis, Christian-Albrechts-Universit\u00e4t]."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.femsec.2003.12.013","article-title":"Distribution of Prokaryotic Genetic Diversity in Athalassohaline Lakes of the Atacama Desert, Northern Chile","volume":"48","author":"Demergasso","year":"2004","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_8","first-page":"114","article-title":"Flora and Vegetation of Ascotan Saltmarsh, Northern Chilean Andes","volume":"60","author":"Teiller","year":"2003","journal-title":"Gayana Bot."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1111\/jfb.12031","article-title":"Phylogenetic and Phylogeographic Analysis of the Genus Orestias (Teleostei: Cyprinodontidae) in the Southern Chilean Altiplano: The Relevance of Ancient and Recent Divergence Processes in Speciation","volume":"82","author":"Vila","year":"2013","journal-title":"J. Fish Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.ygeno.2021.12.018","article-title":"Genome Sequencing and Transcriptomic Analysis of the Andean Killifish Orestias ascotanensis Reveals Adaptation to High-Altitude Aquatic Life","volume":"114","author":"Nardocci","year":"2022","journal-title":"Genomics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.copbio.2021.01.019","article-title":"Metagenomic Tools in Microbial Ecology Research","volume":"67","author":"Li","year":"2021","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.mib.2015.05.005","article-title":"Metagenomics of Extreme Environments","volume":"25","author":"Cowan","year":"2015","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1146\/annurev.micro.57.030502.090759","article-title":"The Uncultured Microbial Majority","volume":"57","author":"Giovannoni","year":"2003","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1093\/bib\/bbv001","article-title":"Environmental Genes and Genomes: Understanding the Differences and Challenges in the Approaches and Software for Their Analyses","volume":"16","author":"Mendoza","year":"2014","journal-title":"Brief. Bioinform."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Xu, G., Zhang, L., Liu, X., Guan, F., Xu, Y., Yue, H., Huang, J.Q., Chen, J., Wu, N., and Tian, J. (2022). Combined Assembly of Long and Short Sequencing Reads Improve the Efficiency of Exploring the Soil Metagenome. BMC Genom., 23.","DOI":"10.1186\/s12864-021-08260-3"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3175","DOI":"10.1038\/s41467-022-30857-9","article-title":"Short- and Long-Read Metagenomics Expand Individualized Structural Variations in Gut Microbiomes","volume":"13","author":"Chen","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/s00284-005-0241-5","article-title":"Diverse UV-B Resistance of Culturable Bacterial Community from High-Altitude Wetland Water","volume":"52","author":"Zenoff","year":"2006","journal-title":"Curr. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1029\/2008JG000836","article-title":"Prokaryotic Diversity Pattern in High-Altitude Ecosystems of the Chilean Altiplano","volume":"115","author":"Demergasso","year":"2010","journal-title":"J. Geophys. Res. Biogeosci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1007\/s00128-009-9659-y","article-title":"Isolation of Arsenite-Oxidizing Bacteria from Arsenic-Enriched Sediments from Camarones River, Northern Chile","volume":"82","author":"Valenzuela","year":"2009","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1007\/s001280000148","article-title":"Copper Contamination and Cadmium, Silver, and Zinc Concentrations in the Digestive Glands of American Lobster (Homarus americanus) from the Inner Bay of Fundy, Atlantic Canada","volume":"65","author":"Chou","year":"2000","journal-title":"Bull. Environ. Contam. Toxicol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cpbi.100","article-title":"QIIME 2 Enables Comprehensive End-to-End Analysis of Diverse Microbiome Data and Comparative Studies with Publicly Available Data","volume":"70","author":"Estaki","year":"2020","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2666","DOI":"10.1093\/bioinformatics\/bty149","article-title":"NanoPack: Visualizing and Processing Long-Read Sequencing Data","volume":"34","author":"Schultz","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ondov, B.D., Treangen, T.J., Melsted, P., Mallonee, A.B., Bergman, N.H., Koren, S., and Phillippy, A.M. (2016). Mash: Fast Genome and Metagenome Distance Estimation Using MinHash. Genome Biol., 17.","DOI":"10.1186\/s13059-016-0997-x"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.21105\/joss.01762","article-title":"Mashtree: A Rapid Comparison of Whole Genome Sequence Files","volume":"4","author":"Katz","year":"2019","journal-title":"J. Open Source Softw."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e00039-18","DOI":"10.1128\/mSystems.00039-18","article-title":"Nonpareil 3: Fast Estimation of Metagenomic Coverage and Sequence Diversity","volume":"3","author":"Gunturu","year":"2018","journal-title":"mSystems"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"11257","DOI":"10.1038\/ncomms11257","article-title":"Fast and Sensitive Taxonomic Classification for Metagenomics with Kaiju","volume":"7","author":"Menzel","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wood, D.E., Lu, J., and Langmead, B. (2019). Improved Metagenomic Analysis with Kraken 2. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1891-0"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1038\/s41592-020-00971-x","article-title":"MetaFlye: Scalable Long-Read Metagenome Assembly Using Repeat Graphs","volume":"17","author":"Kolmogorov","year":"2020","journal-title":"Nat. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Wick, R.R., and Holt, K.E. (2022). Polypolish: Short-Read Polishing of Long-Read Bacterial Genome Assemblies. PLoS Comput. Biol., 18.","DOI":"10.1371\/journal.pcbi.1009802"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1093\/bioinformatics\/btt476","article-title":"The MaSuRCA Genome Assembler","volume":"29","author":"Zimin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: Quality Assessment Tool for Genome Assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Uritskiy, G.V., DiRuggiero, J., and Taylor, J. (2018). MetaWRAP\u2014A Flexible Pipeline for Genome-Resolved Metagenomic Data Analysis. Microbiome, 6.","DOI":"10.1186\/s40168-018-0541-1"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e7359","DOI":"10.7717\/peerj.7359","article-title":"MetaBAT 2: An Adaptive Binning Algorithm for Robust and Efficient Genome Reconstruction from Metagenome Assemblies","volume":"7","author":"Kang","year":"2019","journal-title":"PeerJ"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1038\/nmeth.3103","article-title":"Binning Metagenomic Contigs by Coverage and Composition","volume":"11","author":"Alneberg","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1093\/bioinformatics\/btv638","article-title":"MaxBin 2.0: An Automated Binning Algorithm to Recover Genomes from Multiple Metagenomic Datasets","volume":"32","author":"Wu","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5315","DOI":"10.1093\/bioinformatics\/btac672","article-title":"GTDB-Tk v2: Memory Friendly Classification with the Genome Taxonomy Database","volume":"38","author":"Chaumeil","year":"2022","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8883","DOI":"10.1093\/nar\/gkaa621","article-title":"DRAM for Distilling Microbial Metabolism to Automate the Curation of Microbiome Function","volume":"48","author":"Shaffer","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1093\/bioinformatics\/btv584","article-title":"SUPER-FOCUS: A Tool for Agile Functional Analysis of Shotgun Metagenomic Data","volume":"32","author":"Silva","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1093\/nar\/gkt1252","article-title":"BacMet: Antibacterial Biocide and Metal Resistance Genes Database","volume":"42","author":"Pal","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4453","DOI":"10.1093\/bioinformatics\/btz305","article-title":"RAxML-NG: A Fast, Scalable and User-Friendly Tool for Maximum Likelihood Phylogenetic Inference","volume":"35","author":"Kozlov","year":"2019","journal-title":"Bioinformatics"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree of Life (ITOL) v5: An Online Tool for Phylogenetic Tree Display and Annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","article-title":"High Throughput ANI Analysis of 90K Prokaryotic Genomes Reveals Clear Species Boundaries","volume":"9","author":"Jain","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"W282","DOI":"10.1093\/nar\/gky467","article-title":"The Microbial Genomes Atlas (MiGA) Webserver: Taxonomic and Gene Diversity Analysis of Archaea and Bacteria at the Whole Genome Level","volume":"46","author":"Gunturu","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1002\/bit.20347","article-title":"Group-Specific Primer and Probe Sets to Detect Methanogenic Communities Using Quantitative Real-Time Polymerase Chain Reaction","volume":"89","author":"Yu","year":"2005","journal-title":"Biotechnol. Bioeng."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5445","DOI":"10.1128\/AEM.00726-09","article-title":"Culture-Independent Characterization of Archaeal Biodiversity in Swine Confinement Building Bioaerosols","volume":"75","author":"Gilbert","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1101\/gr.186072.114","article-title":"CheckM: Assessing the Quality of Microbial Genomes Recovered from Isolates, Single Cells, and Metagenomes","volume":"25","author":"Parks","year":"2015","journal-title":"Genome Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.syapm.2018.11.002","article-title":"Candidatus Krumholzibacterium Zodletonense gen. nov., sp nov, the First Representative of the Candidate Phylum Krumholzibacteriota Phyl. Nov. Recovered from an Anoxic Sulfidic Spring Using Genome Resolved Metagenomics","volume":"42","author":"Youssef","year":"2019","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Tarn, J., Peoples, L.M., Hardy, K., Cameron, J., and Bartlett, D.H. (2016). Identification of Free-Living and Particle-Associated Microbial Communities Present in Hadal Regions of the Mariana Trench. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00665"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"3480","DOI":"10.1038\/s41396-021-01026-5","article-title":"Degradation of Biological Macromolecules Supports Uncultured Microbial Populations in Guaymas Basin Hydrothermal Sediments","volume":"15","author":"Borton","year":"2021","journal-title":"ISME J."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Suarez, C., Hackl, T., Wilen, B.-M., Persson, F., Hagelia, P., Jetten, M., and Martins, P.D. (2023). Novel and Unusual Genes for Nitrogen and Metal Cycling in Planctomycetota- and KSB1-Affiliated Metagenome-Assembled Genomes Reconstructed from a Marine Subsea Tunnel. FEMS Microbiol. Lett., 370.","DOI":"10.1093\/femsle\/fnad049"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e13241","DOI":"10.7717\/peerj.13241","article-title":"Phylogeny, Distribution and Potential Metabolism of Candidate Bacterial Phylum KSB1","volume":"10","author":"Li","year":"2022","journal-title":"PeerJ"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Ji, Y., Zhang, P., Zhou, S., Gao, P., Wang, B., and Jiang, J. (2022). Widespread but Poorly Understood Bacteria: Candidate Phyla Radiation. Microorganisms, 10.","DOI":"10.3390\/microorganisms10112232"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/nature14486","article-title":"Unusual Biology across a Group Comprising More than 15% of Domain Bacteria","volume":"523","author":"Brown","year":"2015","journal-title":"Nature"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1007\/s13157-014-0560-z","article-title":"Diversity of Desulfobacteriaceae and Overall Activity of Sulfate-Reducing Microorganisms in and around a Salt Pan in a Southern California Coastal Wetland","volume":"34","author":"Jackson","year":"2014","journal-title":"Wetlands"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/ismej.2007.79","article-title":"Ammonia Oxidation and Ammonia-Oxidizing Bacteria and Archaea from Estuaries with Differing Histories of Hypoxia","volume":"1","author":"Caffrey","year":"2007","journal-title":"ISME J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/ismej.2016.155","article-title":"Co-Occurrence of Antibiotic and Metal Resistance Genes Revealed in Complete Genome Collection","volume":"11","author":"Li","year":"2017","journal-title":"ISME J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1038\/s41598-017-00896-0","article-title":"Arsenic Metabolism in High Altitude Modern Stromatolites Revealed by Metagenomic Analysis","volume":"7","author":"Kurth","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1099\/jmm.0.023036-0","article-title":"Bacterial Antimicrobial Metal Ion Resistance","volume":"64","author":"Hobman","year":"2015","journal-title":"J. Med. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Altermann, E. (2012). Tracing Lifestyle Adaptation in Prokaryotic Genomes. Front. Microbiol., 3.","DOI":"10.3389\/fmicb.2012.00048"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1127\/1863-9135\/2013\/0393","article-title":"Bacterial and Archaeal Diversity in High Altitude Wetlands of the Chilean Altiplano","volume":"182","author":"Dorador","year":"2013","journal-title":"Fundam. Appl. Limnol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/s00792-008-0153-y","article-title":"Novelty and Spatio-Temporal Heterogeneity in the Bacterial Diversity of Hypersaline Lake Tebenquiche (Salar de Atacama)","volume":"12","author":"Demergasso","year":"2008","journal-title":"Extremophiles"},{"key":"ref_65","first-page":"291","article-title":"Unique Clusters of Archaea in Salar de Huasco, an Athalassohaline Evaporitic Basin of the Chilean Altiplano","volume":"73","author":"Dorador","year":"2010","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1007\/s11274-013-1399-9","article-title":"A Meta-Analysis of the Publicly Available Bacterial and Archaeal Sequence Diversity in Saline Soils","volume":"29","author":"Ma","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1139\/w99-122","article-title":"The Effects of Ultraviolet Radiation on the Moderate Halophile Halomonas elongata and the Extreme Halophile Halobacterium salinarum","volume":"46","author":"Martin","year":"2000","journal-title":"Can. J. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.2217\/fmb.13.108","article-title":"Microbiology and Epidemiology of Halomonas Species","volume":"8","author":"Kim","year":"2013","journal-title":"Future Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1111\/1751-7915.14170","article-title":"Marinobacter: A Case Study in Bioelectrochemical Chassis Evaluation","volume":"16","author":"Bird","year":"2023","journal-title":"Microb. Biotechnol."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"He, Y., He, L., Wang, Z., Liang, T., Sun, S., and Liu, X. (2022). Salinity Shapes the Microbial Communities in Surface Sediments of Salt Lakes on the Tibetan Plateau, China. Water, 14.","DOI":"10.3390\/w14244043"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.femsre.2004.10.005","article-title":"Haloalkaliphilic Sulfur-Oxidizing Bacteria in Soda Lakes","volume":"29","author":"Sorokin","year":"2005","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1099\/00221287-144-6-1601","article-title":"Psychroflexus torquis gen. nov., sp. nov. a Psychrophilic Species from Antarctic Sea Ice, and Reclassification of Flavobacterium Gondwanense (Dobson et Al. 1993) as Psychroflexus gondwanense gen. nov., comb. nov","volume":"144","author":"Bowman","year":"1998","journal-title":"Microbiology"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1029\/2008JG000837","article-title":"Diversity of Bacteroidetes in High-Altitude Saline Evaporitic Basins in Northern Chile","volume":"114","author":"Dorador","year":"2009","journal-title":"J. Geophys. Res. Biogeosci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"2598","DOI":"10.1099\/ijsem.0.001981","article-title":"Aliifodinibius salicampi sp. nov., a Moderately Halophilic Bacterium Isolated from a Grey Saltern","volume":"67","author":"Cho","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1099\/ijsem.0.003765","article-title":"Aliifodinibius saliphilus sp. Nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Crystallizing Pond of a Saltern","volume":"70","author":"Cho","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1099\/ijs.0.043869-0","article-title":"Aliifodinibius roseus gen. nov., sp. nov., and Aliifodinibius sediminis sp. nov., Two Moderately Halophilic Bacteria Isolated from Salt Mine Samples","volume":"63","author":"Wang","year":"2013","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1099\/ijs.0.040139-0","article-title":"Halanaerobium sehlinense sp. nov., an Extremely Halophilic, Fermentative, Strictly Anaerobic Bacterium from Sediments of the Hypersaline Lake Sehline Sebkha","volume":"63","author":"Abdeljabbar","year":"2013","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Boltyanskaya, Y., Zhilina, T., Grouzdev, D., Detkova, E., Pimenov, N., and Kevbrin, V. (2023). Halanaerobium polyolivorans sp. nov.\u2014A Novel Halophilic Alkalitolerant Bacterium Capable of Polyol Degradation: Physiological Properties and Genomic Insights. Microorganisms, 11.","DOI":"10.3390\/microorganisms11092325"},{"key":"ref_79","unstructured":"Oren, A. (2019). Encyclopedia of Life Sciences, John Wiley & Sons, Ltd."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Rosenberg, E., DeLong, E.F., Lory, S., Stackebrandt, E., and Thompson, F. (2014). The Prokaryotes: Other Major Lineages of Bacteria and the Archaea, Springer.","DOI":"10.1007\/978-3-642-38954-2"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Liu, G., Gong, Z., and Li, Q. (2020). Variations in Gut Bacterial Communities between Lesser White-Fronted Geese Wintering at Caizi and Shengjin Lakes in China. Microbiologyopen, 9.","DOI":"10.1002\/mbo3.1037"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Zhang, S. (2023). Altered Gut Archaeal Communities in Anser Erythropus Populations Wintering at Shengjin and Caizi Lakes in China. Pak. J. Zool., 1\u201310.","DOI":"10.17582\/journal.pjz\/20221208061226"},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Eissler, Y., Dorador, C., Kieft, B., Molina, V., and Hengst, M. (2020). Virus and Potential Host Microbes from Viral-Enriched Metagenomic Characterization in the High-Altitude Wetland, Salar de Huasco, Chile. Microorganisms, 8.","DOI":"10.3390\/microorganisms8071077"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s00792-020-01210-z","article-title":"Purification and Characterization of Two Thermostable Xylanases from a Halotolerant Bacillus sp. Asc6BA Isolated from Salar de Ascot\u00e1n, Atacama Desert","volume":"25","author":"Contreras","year":"2021","journal-title":"Extremophiles"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1139\/cjm-2017-0523","article-title":"Arsenate-Dependent Growth Is Independent of an Arra Mechanism of Arsenate Respiration in the Termite Hindgut Isolate Citrobacter sp. Strain TSA-1","volume":"64","author":"Blum","year":"2018","journal-title":"Can. J. Microbiol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Castro-Severyn, J., Remonsellez, F., Valenzuela, S.L., Salinas, C., Fortt, J., Aguilar, P., Pardo-Est\u00e9, C., Dorador, C., Quatrini, R., and Molina, F. (2017). Comparative Genomics Analysis of a New Exiguobacterium Strain from Salar de Huasco Reveals a Repertoire of Stress-Related Genes and Arsenic Resistance. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00456"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/11\/2819\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:25:29Z","timestamp":1760131529000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/11\/11\/2819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,20]]},"references-count":86,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,11]]}},"alternative-id":["microorganisms11112819"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms11112819","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,20]]}}}