{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T03:20:28Z","timestamp":1792466428196,"version":"4.5.0"},"reference-count":30,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T00:00:00Z","timestamp":1703721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICMR-VCRC Intra-mural","award":["IM 1911"],"award-info":[{"award-number":["IM 1911"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>A survey was conducted to determine the human tick-borne bacterial infections in the nymphs which were collected from Western Ghats\u2019 fringe forest areas. Tick nymphs were collected using the flagging method from the villages where cases Kyasanur Forest Disease (KFD) were previously reported in the states of Goa, Karnataka and Maharashtra. A total of 200 tick pools consisting of 4587 nymphs were tested by PCR for the detection of bacteria of public health importance, such as Coxiella burnetii and Rickettsia spp. Of these, four pools (4.8%) in Karnataka and three pools (4.4%) in Maharashtra were positive for Coxiella burnetii, while none of the samples from Goa state were positive. Rickettsia spp. were positively obtained from Maharashtra (51.5%), Goa (35.42%) and Karnataka (26.19%). The sequence results of Rickettsia spp. showed similarity to the spotted fever group Candidatus Rickettsia shennongii, Rickettsia conorii subsp. heilongjiangensis and Rickettsia spp. strain koreansis. Individuals are entering into the forest areas for various reasons are more likely to infect with Coxiella burnetii. and Rickettsia spp.<\/jats:p>","DOI":"10.3390\/microorganisms12010052","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T09:56:27Z","timestamp":1703757387000},"page":"52","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Molecular Detection of Bacterial Infections of Public Health Importance in Hard Tick (Ixodidae) Nymphs Collected from the Forest Fringes of Western Ghats in the Goa, Karnataka and Maharashtra States of India"],"prefix":"10.3390","volume":"12","author":[{"given":"Gnanasekar","family":"Ragini","sequence":"first","affiliation":[{"name":"Climate Change, GIS and VBD Stratification\/Mapping, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health & Family Welfare, GOI, Medical Complex, Indira Nagar, Puducherry 605 006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari","family":"Raju","sequence":"additional","affiliation":[{"name":"Climate Change, GIS and VBD Stratification\/Mapping, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health & Family Welfare, GOI, Medical Complex, Indira Nagar, Puducherry 605 006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0012-695X","authenticated-orcid":false,"given":"Ranganathan","family":"Krishnamoorthi","sequence":"additional","affiliation":[{"name":"Climate Change, GIS and VBD Stratification\/Mapping, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health & Family Welfare, GOI, Medical Complex, Indira Nagar, Puducherry 605 006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayyanar","family":"Elango","sequence":"additional","affiliation":[{"name":"Division of Vector Biology and Control, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health & Family Welfare, GOI, Medical Complex, Indira Nagar, Puducherry 605 006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subramanian","family":"Muthukumaravel","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology, ICMR-Vector Control Research Centre, Department of Health Research, Ministry of Health & Family Welfare, GOI, Medical Complex, Indira Nagar, Puducherry 605 006, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2485-7581","authenticated-orcid":false,"given":"Ashwani","family":"Kumar","sequence":"additional","affiliation":[{"name":"Centre for Global Health Research, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 605102, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1007\/s00436-021-07114-x","article-title":"Update on Prevalence and Distribution Pattern of Tick-Borne Diseases among Humans in India: A Review","volume":"120","author":"Negi","year":"2021","journal-title":"Parasitol. 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