{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:04:26Z","timestamp":1787774666035,"version":"build-2784847793"},"reference-count":118,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"E022 Program, National Institute of Pediatrics, Mexico City, Mexico (Recursos Fiscales para la Investigaci\u00f3n)"},{"name":"INP 021\/2022"},{"name":"HIM\/2019\/036 SSA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Several microaerophilic parasites such as Giardia lamblia, Trichomonas vaginalis, and Plasmodium falciparum are major disease-causing organisms and are responsible for spreading infections worldwide. Despite significant progress made in understanding the metabolism and molecular biology of microaerophilic parasites, chemotherapeutic treatment to control it has seen limited progress. A current proposed strategy for drug discovery against parasitic diseases is the identification of essential key enzymes of metabolic pathways associated with the parasite\u2019s survival. In these organisms, glucose-6-phosphate dehydrogenase::6-phosphogluconolactonase (G6PD:: 6PGL), the first enzyme of the pentose phosphate pathway (PPP), is essential for its metabolism. Since G6PD:: 6PGL provides substrates for nucleotides synthesis and NADPH as a source of reducing equivalents, it could be considered an anti-parasite drug target. This review analyzes the anaerobic energy metabolism of G. lamblia, T. vaginalis, and P. falciparum, with a focus on glucose metabolism through the pentose phosphate pathway and the significance of the fused G6PD:: 6PGL enzyme as a therapeutic target in the search for new drugs.<\/jats:p>","DOI":"10.3390\/microorganisms12010112","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T03:43:00Z","timestamp":1704426180000},"page":"112","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Fused Enzyme Glucose-6-Phosphate Dehydrogenase::6-Phosphogluconolactonase (G6PD::6PGL) as a Potential Drug Target in Giardia lamblia, Trichomonas vaginalis, and Plasmodium falciparum"],"prefix":"10.3390","volume":"12","author":[{"given":"Laura","family":"Morales-Luna","sequence":"first","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Gen\u00e9tica, Instituto Nacional de Pediatr\u00eda, Secretar\u00eda de Salud, Mexico City 04530, Mexico"},{"name":"Posgrado en Ciencias Biol\u00f3gicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4651-7892","authenticated-orcid":false,"given":"Montserrat","family":"V\u00e1zquez-Bautista","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Gen\u00e9tica, Instituto Nacional de Pediatr\u00eda, Secretar\u00eda de Salud, Mexico City 04530, Mexico"},{"name":"Programa de Posgrado en Biomedicina y Biotecnolog\u00eda Molecular, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6137-6499","authenticated-orcid":false,"given":"V\u00edctor","family":"Mart\u00ednez-Rosas","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Gen\u00e9tica, Instituto Nacional de Pediatr\u00eda, Secretar\u00eda de Salud, Mexico City 04530, Mexico"},{"name":"Programa de Posgrado en Biomedicina y Biotecnolog\u00eda Molecular, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam Abigail","family":"Rojas-Alarc\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Gen\u00e9tica, Instituto Nacional de Pediatr\u00eda, Secretar\u00eda de Salud, Mexico City 04530, Mexico"},{"name":"Programa de Posgrado en Biomedicina y Biotecnolog\u00eda Molecular, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7950-4911","authenticated-orcid":false,"given":"Daniel","family":"Ortega-Cuellar","sequence":"additional","affiliation":[{"name":"Laboratorio de Nutrici\u00f3n Experimental, Instituto Nacional de Pediatr\u00eda, Secretar\u00eda de Salud, Mexico City 04530, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail","family":"Gonz\u00e1lez-Valdez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Molecular y Biotecnolog\u00eda, Instituto de Investigaciones Biom\u00e9dicas, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1451-8754","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"P\u00e9rez de la Cruz","sequence":"additional","affiliation":[{"name":"Neurobiochemistry and Behavior Laboratory, National Institute of Neurology and Neurosurgery \u201cManuel Velasco Su\u00e1rez\u201d, Mexico City 14269, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4611-4770","authenticated-orcid":false,"given":"Roberto","family":"Arreguin-Espinosa","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica de Biomacromol\u00e9culas, Instituto de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Rodr\u00edguez-Bustamante","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica de Biomacromol\u00e9culas, Instituto de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"},{"name":"Departamento de Bioingenier\u00eda, Escuela de Ingenier\u00eda y Ciencias, Tecnol\u00f3gico de Monterrey, Monterrey 64849, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eden","family":"Rodr\u00edguez-Flores","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica de Biomacromol\u00e9culas, Instituto de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3939-6930","authenticated-orcid":false,"given":"Beatriz","family":"Hern\u00e1ndez-Ochoa","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunoqu\u00edmica, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Secretar\u00eda de Salud, Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2095-9182","authenticated-orcid":false,"given":"Sa\u00fal","family":"G\u00f3mez-Manzo","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica Gen\u00e9tica, Instituto Nacional de 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Spectr."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/j.ijpara.2021.08.007","article-title":"Taxonomy and molecular epidemiology of Cryptosporidium and Giardia\u2014A 50 year perspective (1971\u20132021)","volume":"51","author":"Ryan","year":"2021","journal-title":"Int. J. Parasitol."},{"key":"ref_3","first-page":"406","article-title":"Trichomonas vaginalis: Clinical relevance, pathogenicity and diagnosis","volume":"42","author":"Edwards","year":"2016","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"548","DOI":"10.2471\/BLT.18.228486","article-title":"Chlamydia, gonorrhoea, trichomoniasis and syphilis: Global prevalence and incidence estimates, 2016","volume":"97","author":"Rowley","year":"2019","journal-title":"Bull. World Health Organ."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1363\/3600604","article-title":"Sexually transmitted diseases among American youth: Incidence and prevalence estimates, 2000","volume":"36","author":"Weinstock","year":"2004","journal-title":"Perspect. Sex Reprod. Health"},{"key":"ref_6","first-page":"29","article-title":"Plasmodium falciparum malaria","volume":"151","author":"Shetty","year":"1999","journal-title":"J. State Med. Soc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1001\/jama.2021.21468","article-title":"Malaria","volume":"327","author":"Walter","year":"2022","journal-title":"JAMA"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Adam, R.D. (2021). Giardia duodenalis: Biology and Pathogenesis. Clin. Microbiol. Rev., 34.","DOI":"10.1128\/CMR.00024-19"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1051\/parasite\/2020047","article-title":"Update on Giardia: Highlights from the seventh International Giardia and Cryptosporidium Conference","volume":"27","author":"Buret","year":"2020","journal-title":"Parasite"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1016\/j.pt.2017.08.001","article-title":"The Intersection of Immune Responses, Microbiota, and Pathogenesis in Giardiasis","volume":"33","author":"Fink","year":"2017","journal-title":"Trends Parasitol."},{"key":"ref_11","first-page":"37","article-title":"Nitroimidazole-refractory giardiasis: A growing problem requiring rational solutions","volume":"24","author":"Carter","year":"2018","journal-title":"Clin. Microbiol. Infect. Off. Publ. Eur. Soc. Clin. Microbiol. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.ijpddr.2021.05.003","article-title":"In vitro selection of Giardia duodenalis for Albendazole resistance identifies a \u03b2-tubulin mutation at amino acid E198K","volume":"16","author":"Su","year":"2021","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1556\/1886.2021.00009","article-title":"Antimicrobial resistance of the enteric protozoon Giardia duodenalis\u2014A narrative review","volume":"11","author":"Frickmann","year":"2021","journal-title":"Eur. J. Microbiol. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1097\/QCO.0000000000000877","article-title":"Giardia and growth impairment in children in high-prevalence settings: Consequence or co-incidence?","volume":"35","author":"Dougherty","year":"2022","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Menezes, S.A., and Tasca, T. (2023). Essential Oils and Terpenic Compounds as Potential Hits for Drugs against Amitochondriate Protists. Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8010037"},{"key":"ref_16","unstructured":"World Health Organization (2012). Global Report for Research on Infectious Diseases of Poverty."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.diagmicrobio.2012.05.003","article-title":"Performance of rapid immunochromatographic assay in the diagnosis of Trichomoniasis vaginalis","volume":"74","author":"Hegazy","year":"2012","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_18","unstructured":"World Health Organization (2018). World Health Statistics 2016: Monitoring Health for the SDGs, Sustainable Development Goals."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Crucitti, T., Jespers, V., Mulenga, C., Khondowe, S., Vandepitte, J., and Buv\u00e9, A. (2011). Non-sexual transmission of Trichomonas vaginalis in adolescent girls attending school in Ndola, Zambia. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0016310"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Francis, S.C., Mthiyane, T.N., Baisley, K., Mchunu, S.L., Ferguson, J.B., Smit, T., Crucitti, T., Gareta, D., Dlamini, S., and Mutevedzi, T. (2018). Prevalence of sexually transmitted infections among young people in South Africa: A nested survey in a health and demographic surveillance site. PLoS Med., 15.","DOI":"10.1371\/journal.pmed.1002512"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1177\/0956462421990218","article-title":"Mother-to-child transmission of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis in HIV-infected pregnant women in South Africa","volume":"32","author":"Peters","year":"2021","journal-title":"Int. J. STD AIDS"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"S152","DOI":"10.1093\/cid\/ciac030","article-title":"Diagnosis and Management of Trichomonas vaginalis: Summary of Evidence Reviewed for the 2021 Centers for Disease Control and Prevention Sexually Transmitted Infections Treatment Guidelines","volume":"74","author":"Kissinger","year":"2022","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1111\/jdv.17461","article-title":"Trichomonas vaginalis and growing concern over drug resistance: A systematic review","volume":"35","author":"Lisboa","year":"2021","journal-title":"J. Eur. Acad. Dermatol. Venereol. JEADV"},{"key":"ref_24","first-page":"198","article-title":"Tricomoniasis: Una vision amplia","volume":"27","year":"2014","journal-title":"Latreia"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.rr7004a1","article-title":"Sexually Transmitted Infections Treatment Guidelines, 2021","volume":"70","author":"Workowski","year":"2021","journal-title":"MMWR Recomm. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1017\/S0031182020001237","article-title":"A systematic review of the literature on mechanisms of 5-nitroimidazole resistance in Trichomonas vaginalis","volume":"147","author":"Graves","year":"2020","journal-title":"Parasitology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1097\/QCO.0000000000000867","article-title":"Update on pathogenesis, management, and control of Plasmodium vivax","volume":"35","author":"Khan","year":"2022","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_28","unstructured":"World Health Organization (2023). Global Report on Neglected Tropical Diseases 2023."},{"key":"ref_29","unstructured":"World Health Organization (2022). World Malaria Report 2022."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1186\/s12936-020-03336-z","article-title":"Pathophysiology and neurologic sequelae of cerebral malaria","volume":"19","author":"Schiess","year":"2020","journal-title":"Malar. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1186\/s12936-022-04130-9","article-title":"Why Plasmodium vivax and Plasmodium falciparum are so different? A tale of two clades and their species diversities","volume":"21","author":"Escalante","year":"2022","journal-title":"Malar. J."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Su, X.-Z., Zhang, C., and Joy, D.A. (2020). Host-Malaria Parasite Interactions and Impacts on Mutual Evolution. Front. Cell. Infect. Microbiol., 10.","DOI":"10.3389\/fcimb.2020.587933"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1962","DOI":"10.1056\/NEJMc1713777","article-title":"Evidence of Artemisinin-Resistant Plasmodium falciparum Malaria in Eastern India","volume":"379","author":"Das","year":"2018","journal-title":"New Engl. J. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9946","DOI":"10.1038\/s41598-021-89295-0","article-title":"Artemisinin combination therapy fails even in the absence of Plasmodium falciparum kelch13 gene polymorphism in Central India","volume":"11","author":"Das","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1038\/s41564-023-01461-4","article-title":"Plasmodium falciparum resistant to artemisinin and diagnostics have emerged in Ethiopia","volume":"8","author":"Fola","year":"2023","journal-title":"Nat. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/nature09107","article-title":"Thousands of chemical starting points for antimalarial lead identification","volume":"465","author":"Gamo","year":"2010","journal-title":"Nature"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nature09099","article-title":"Chemical genetics of Plasmodium falciparum","volume":"465","author":"Guiguemde","year":"2010","journal-title":"Nature"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1126\/science.1211936","article-title":"Imaging of Plasmodium liver stages to drive next-generation antimalarial drug discovery","volume":"334","author":"Meister","year":"2011","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1021\/acsinfecdis.3c00138","article-title":"A Pyridyl-Furan Series Developed from the Open Global Health Library Block Red Blood Cell Invasion and Protein Trafficking in Plasmodium falciparum through Potential Inhibition of the Parasite\u2019s PI4KIIIB Enzyme","volume":"9","author":"Ling","year":"2023","journal-title":"ACS Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"134","DOI":"10.2174\/1872213X13666190618124901","article-title":"Giardiasis: An Overview","volume":"13","author":"Leung","year":"2019","journal-title":"Recent Patents Inflamm. Allergy Drug Discov."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1517\/14656566.8.12.1885","article-title":"Giardiasis: A pharmacotherapy review","volume":"8","author":"Escobedo","year":"2007","journal-title":"Expert Opin. Pharmacother."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1517\/14740338.2.6.529","article-title":"Efficacy of antigiardial drugs","volume":"2","author":"Wright","year":"2003","journal-title":"Expert Opin. Drug Saf."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1093\/cid\/ciz146","article-title":"Trichomonas vaginalis Virus Among Women with Trichomoniasis and Associations with Demographics, Clinical Outcomes, and Metronidazole Resistance","volume":"69","author":"Graves","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/j.1365-2958.2009.06675.x","article-title":"Trichomonas vaginalis: Metronidazole and other nitroimidazole drugs are reduced by the flavin enzyme thioredoxin reductase and disrupt the cellular redox system. Implications for nitroimidazole toxicity and resistance","volume":"72","author":"Leitsch","year":"2009","journal-title":"Mol. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1093\/jac\/dkr192","article-title":"Pyruvate:ferredoxin oxidoreductase and thioredoxin reductase are involved in 5-nitroimidazole activation while flavin metabolism is linked to 5-nitroimidazole resistance in Giar-dia lamblia","volume":"66","author":"Leitsch","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.ijpddr.2012.04.002","article-title":"Nitroimidazole drugs vary in their mode of action in the human parasite Giardia lamblia","volume":"2","author":"Leitsch","year":"2012","journal-title":"Int. J. Parasitol. Drugs Drug Resist."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4643","DOI":"10.1128\/AAC.00384-11","article-title":"Impaired parasite attachment as fitness cost of metronidazole resistance in Giardia lamblia","volume":"55","author":"Millman","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1128\/AAC.01548-06","article-title":"A novel Giardia lamblia nitroreductase, GLNR1, interacts with nitazoxanide and other thiazolides","volume":"51","author":"Wastling","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1093\/jac\/dkn142","article-title":"Identification of differentially expressed genes in a Giardia lamblia WB C6 clone resistant to nitazoxanide and metronidazole","volume":"62","author":"Ley","year":"2008","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1093\/jac\/dkt106","article-title":"Metabolism of nitro drugs metronidazole and nitazoxanide in Giardia lamblia: Characterization of a novel nitroreductase (GlNR2)","volume":"68","author":"Schildknecht","year":"2013","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ijpddr.2023.03.004","article-title":"Transcriptomic analysis of albend-azole resistance in human diarrheal parasite Giardia duodenalis","volume":"22","author":"Su","year":"2023","journal-title":"Int. J. Parasitology. Drugs Drug Resist."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"106870","DOI":"10.1016\/j.actatropica.2023.106870","article-title":"Insight into molecular diagnosis for antimalarial drug resistance of Plasmodium falciparum parasites: A review","volume":"241","author":"Zhong","year":"2023","journal-title":"Acta Trop."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/j.tips.2006.09.005","article-title":"Transporters involved in resistance to antimalarial drugs","volume":"27","author":"Valderramos","year":"2006","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1586\/14787210.2015.1031744","article-title":"Fighting fire with fire: Mass antimalarial drug administrations in an era of antimalarial resistance","volume":"13","author":"Dondorp","year":"2015","journal-title":"Expert Rev. Anti. Infec. Ther."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1073\/pnas.91.3.1143","article-title":"Selection for mefloquine resistance in Plasmodium falciparum is linked to am-plification of the pfmdr1 gene and crossresistance to halofantrine and quinine","volume":"91","author":"Cowman","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.actatropica.2005.04.008","article-title":"Contribution of the pfmdr1 gene to antimalarial drug-resistance","volume":"94","author":"Duraisingh","year":"2005","journal-title":"Acta Trop."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1073\/pnas.1211205110","article-title":"Genetic loci associated with delayed clearance of Plasmodium falciparum following artemisinin treatment in Southeast Asia","volume":"110","author":"Clark","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/nature12876","article-title":"A mo-lecular marker of artemisinin-resistant Plasmodium falciparum malaria","volume":"505","author":"Ariey","year":"2014","journal-title":"Nature"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1093\/infdis\/jiu491","article-title":"Independent Emergence of artemisinin resistance mutations among Plasmodium falciparum in Southeast Asia","volume":"211","author":"Jacob","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2917","DOI":"10.1016\/j.celrep.2019.10.095","article-title":"Decreased K13 Abundance Reduces Hemoglobin Catabolism and Proteotoxic Stress, Underpinning Artemisinin Resistance","volume":"29","author":"Yang","year":"2019","journal-title":"Cell Rep."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jgar.2023.08.012","article-title":"Artemisinin resistance in P. falciparum: Probing the interacting partners of Kelch13 protein in parasite","volume":"35","author":"Atul","year":"2023","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/nrd3480","article-title":"How were new medicines discovered?","volume":"10","author":"Swinney","year":"2011","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1038\/clpt.2012.236","article-title":"Phenotypic vs. target-based drug discovery for first-in-class medicines","volume":"93","author":"Swinney","year":"2013","journal-title":"Clin. Pharmacol. Ther."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1039\/c2mb25468a","article-title":"Target identification of small molecules based on chemical biology approaches","volume":"9","author":"Futamura","year":"2013","journal-title":"Mol. Biosyst."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"7719","DOI":"10.1021\/jm400362b","article-title":"Drug discovery for neglected diseases: Molecular target-based and phenotypic approaches","volume":"56","author":"Gilbert","year":"2013","journal-title":"J. Med. Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"7810","DOI":"10.1038\/s41598-017-07612-y","article-title":"Novel giardicidal compounds bearing proton pump inhibitor scaffold proceeding through triosephosphate isomerase inactivation","volume":"7","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"102086","DOI":"10.1016\/j.parint.2020.102086","article-title":"Developing a new drug against trichomoniasis, new inhibitory compounds of the protein triosephosphate isomerase","volume":"76","author":"Brieba","year":"2020","journal-title":"Parasitol. Int."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"8922","DOI":"10.1038\/s41598-019-45529-w","article-title":"On the molecular and cellular effects of omeprazole to further support its effectiveness as an antigiardial drug","volume":"9","author":"Ridaura","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Lazar, I.M., Kontoyianni, M., and Lazar, A.C. (2017). Proteomics for Drug Discovery: Methods and Protocols, Springer.","DOI":"10.1007\/978-1-4939-7201-2"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0166-6851(80)90037-7","article-title":"Energy metabolism of the anaerobic protozoon Giardia lamblia","volume":"1","author":"Lindmark","year":"1980","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1128\/CMR.14.1.150-164.2001","article-title":"Drug Targets and mechanisms of resistance in the anaerobic protozoa","volume":"14","author":"Upcroft","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1146\/annurev.mi.40.100186.000543","article-title":"Microaerophily and oxygen toxicity","volume":"40","author":"Krieg","year":"1986","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1099\/mic.0.26834-0","article-title":"The plasma membrane of microaerophilic protists: Oxidative and nitrosative stress","volume":"150","author":"Lloyd","year":"2004","journal-title":"Microbiology"},{"key":"ref_74","unstructured":"Lloyd, D., and Coombs, G.H. (1989). Biochemistry and Molecular Biology of \u2018Anaerobic\u2019 Protozoa, Harwood Academic Publishers."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1099\/00221287-146-12-3109","article-title":"The microaerophilic flagellate Giardia intestinalis: Oxygen and its reaction products collapse membrane potential and cause cytotoxicity","volume":"146","author":"Lloyd","year":"2000","journal-title":"Microbiology"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0169-4758(96)10075-2","article-title":"The pentose phosphate pathway and parasitic protozoa","volume":"13","author":"Barrett","year":"1997","journal-title":"Parasitol. Today"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.pt.2014.12.003","article-title":"Parasites and biological invasions: Parallels, interactions, and control","volume":"31","author":"Dunn","year":"2015","journal-title":"Trends Parasitol."},{"key":"ref_78","first-page":"1","article-title":"Reactive oxygen species: Training, function and oxidative stress","volume":"36","author":"Carvajal","year":"2019","journal-title":"Rev. Med. Leg. Costa Rica"},{"key":"ref_79","first-page":"1369","article-title":"Introducci\u00f3n al sistema immune. Componentes celulares del sistema immune innato","volume":"12","author":"Sanz","year":"2017","journal-title":"Medicine"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/0166-6851(96)02661-8","article-title":"Characterisation and purification of pyruvate: Ferredoxin oxidoreductase from Giardia duodenalis","volume":"79","author":"Townson","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/0020-7519(90)90133-8","article-title":"The arginine dihydrolase pathway is present in Giardia intestinalis","volume":"20","author":"Schofield","year":"1990","journal-title":"Int. J. Parasitol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0166-6851(92)90197-R","article-title":"The pathway of arginine catabolism in Giardia intestinalis","volume":"51","author":"Schofield","year":"1992","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0020-7519(97)00172-0","article-title":"Anaerobic bacterial metabolism in the ancient eukaryote Giardia duodenalis","volume":"28","author":"Brown","year":"1998","journal-title":"Int. J. Parasitol."},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Morales-Luna, L., Hern\u00e1ndez-Ochoa, B., Ram\u00edrez-Nava, E.J., Mart\u00ednez-Rosas, V., Ortiz-Ram\u00edrez, P., Fern\u00e1ndez-Rosario, F., Gonz\u00e1lez-Valdez, A., C\u00e1rdenas-Rodr\u00edguez, N., Serrano-Posada, H., and Centeno-Leija, S. (2020). Characterizing the Fused TvG6PD::6PGL Protein from the Protozoan Trichomonas vaginalis, and Effects of the NADP+ Molecule on Enzyme Stability. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21144831"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1007\/s10545-008-1015-6","article-title":"The biochemistry, metabolism and inherited defects of the pentose phosphate pathway: A review","volume":"31","author":"Wamelink","year":"2008","journal-title":"J. Inherit. Metab. Dis."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1111\/brv.12140","article-title":"The return of metabolism: Biochemistry and physiology of the pentose phosphate pathway","volume":"90","author":"Stincone","year":"2015","journal-title":"Biol. Rev. Camb. Philos. Soc."},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Morales-Luna, L., Gonz\u00e1lez-Valdez, A., Hern\u00e1ndez-Ochoa, B., Arreguin-Espinosa, R., Ortega-Cuellar, D., Castillo-Rodr\u00edguez, R.A., Mart\u00ednez-Rosas, V., C\u00e1rdenas-Rodr\u00edguez, N., Enr\u00edquez-Flores, S., and Canseco-\u00c1vila, L.M. (2021). Glucose-6-Phosphate Dehydrogenase::6-Phosphogluconolactonase from the Parasite Giardia lamblia. A Molecular and Biochemical Perspective of a Fused Enzyme. Microorganisms, 9.","DOI":"10.3390\/microorganisms9081678"},{"key":"ref_88","unstructured":"Gross, K., Wang, C., and Saltveit, M. (2016). The Commercial Storage of Fruits, Vegetables, and Florist and Nursery Stocks. Agriculture Handbook 66, Agricultural Research Service, United States Department of Agriculture."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"365","DOI":"10.3389\/fendo.2020.00365","article-title":"The Role of the Pentose Phosphate Pathway in Diabetes and Cancer","volume":"11","author":"Ge","year":"2020","journal-title":"Front. Endocrinol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"280","DOI":"10.2174\/18715206113136660337","article-title":"Glucose-6-phosphate dehydrogenase: A biomarker and potential therapeutic target for cancer","volume":"14","author":"Zhang","year":"2014","journal-title":"Anti-Cancer Agents Med. Chem."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"3349","DOI":"10.1128\/AEM.03653-14","article-title":"Mutational Analyses of Glucose Dehydrogenase and Glucose-6-Phosphate Dehydrogenase Genes in Pseudomonas fluorescens Reveal Their Effects on Growth and Alginate Production","volume":"81","author":"Maleki","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1186\/1475-2875-10-193","article-title":"Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drug target during erythrocytic development","volume":"10","author":"Storm","year":"2011","journal-title":"Malar. J."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1111\/j.1742-4658.2006.05174.x","article-title":"Transient silencing of Plasmodium falciparum bifunctional glu-cose-6-phosphate dehydrogenase-6-phosphogluconolactonase","volume":"273","author":"Crooke","year":"2006","journal-title":"FEBS J."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1016\/j.isci.2019.08.047","article-title":"Reserve Flux Capacity in the Pentose Phosphate Pathway by NADPH Binding Is Conserved across Kingdoms","volume":"19","author":"Christodoulou","year":"2019","journal-title":"iScience"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1046\/j.1432-1327.2001.02078.x","article-title":"Glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase. A novel bifunctional enzyme in malaria parasites","volume":"268","author":"Clarke","year":"2001","journal-title":"Eur. J. Biochem."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Morales-Luna, L., Serrano-Posada, H., Gonz\u00e1lez-Valdez, A., Ortega-Cuellar, D., Vanoye-Carlo, A., Hern\u00e1ndez-Ochoa, B., Sierra-Palacios, E., Rufino-Gonz\u00e1lez, Y., Castillo-Rodr\u00edguez, R.A., and de la Cruz, V.P. (2018). Biochemical Characterization and Structural Modeling of Fused Glucose-6-Phosphate Dehydrogenase-Phosphogluconolactonase from Giardia lamblia. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19092518"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/0166-6851(94)00028-X","article-title":"Cloning of the glucose 6-phosphate dehydrogenase gene from Plasmodium falciparum","volume":"64","author":"Wanachiwanawin","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.ejmech.2018.01.044","article-title":"First homology model of Plasmodium falciparum glucose-6-phosphate dehydrogenase: Discovery of selective substrate analog-based inhibitors as novel antimalarial agents","volume":"146","author":"Alencar","year":"2018","journal-title":"Eur. J. Med. Chem."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/0166-6851(81)90100-6","article-title":"Detection of glucose-6-phosphate dehydrogenase in malarial parasites","volume":"2","author":"Hempelmann","year":"1981","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0166-6851(88)90144-2","article-title":"Glucose-6-phosphate dehydrogenase activity of the malaria parasite Plasmodium falciparum","volume":"31","author":"Ling","year":"1988","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0166-6851(90)90099-8","article-title":"Expression and characterization of glucose-6-phosphate dehydrogenase of Plasmodium falciparum","volume":"41","author":"Luzzatto","year":"1990","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0167-4781(94)90269-0","article-title":"A novel glucose 6-phosphate-dehydrogenase in Plasmodium falciparum\u2014cDNA and primary protein structure","volume":"1219","author":"Shahabuddin","year":"1994","journal-title":"Biochim. Biophys. Acta."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0166-6851(02)00298-0","article-title":"A unique insertion in Plasmodium berghei glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase: Evolutionary and functional studies","volume":"127","author":"Clarke","year":"2003","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1042\/BJ20110170","article-title":"Glucose-6-phosphate dehydrogen-ase-6-phosphogluconolactonase: A unique bifunctional enzyme from Plasmodium falciparum","volume":"436","author":"Jortzik","year":"2011","journal-title":"Biochem. J."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"21179","DOI":"10.3390\/ijms151121179","article-title":"The Stability of G6PD Is Affected by Mutations with Different Clinical Phenotypes","volume":"15","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_106","doi-asserted-by":"crossref","unstructured":"Stover, N.A., Dixon, T.A., and Cavalcanti, A.R.O. (2011). Multiple independent fusions of glucose-6-phosphate dehydrogenase with enzymes in the pentose phosphate pathway. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0022269"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1177\/1087057112442382","article-title":"High-throughput screening for small-molecule inhibitors of Plasmodium falciparum glucose-6-phosphate dehydrogenase 6-phosphogluconolactonase","volume":"17","author":"Preuss","year":"2012","journal-title":"J. Biomol. Screen."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"7262","DOI":"10.1021\/jm300833h","article-title":"Discovery of a Plasmodium falciparum glucose-6-phosphate dehydrogenase 6-phosphogluconolactonase inhibitor (R,Z)-N-((1-ethylpyrrolidin-2-yl)methyl)-2-(2-fluorobenzylidene)-3-oxo-3,4-dihydro-2H-benzo[b][1,4]thiazine-6-carboxamide (ML276) that reduces parasite growth in vitro","volume":"55","author":"Preuss","year":"2012","journal-title":"J. Med. Chem."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s12936-019-2651-z","article-title":"Glucose 6-phosphate dehydrogenase 6-phosphogluconolactonase: Characterization of the Plasmodium vivax enzyme and inhibitor studies","volume":"18","author":"Haeussler","year":"2019","journal-title":"Malar. J."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"3808","DOI":"10.1111\/febs.13380","article-title":"Plasmodium falciparumglucose-6-phosphate dehydrogenase 6-phosphogluconolactonase is a potential drug target","volume":"282","author":"Allen","year":"2015","journal-title":"FEBS J."},{"key":"ref_111","unstructured":"Maloney, P., Hedrick, M., Peddibhotla, S., Hershberger, P., Milewski, M., Gosalia, P., Li, L., Preuss, J., Sugarman, E., and Hood, B. (2010). Probe Reports from the NIH Molecular Libraries Program, National Center for Biotechnology Information (US)."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"e0210921","DOI":"10.1128\/aac.02109-21","article-title":"An Optimized Dihydrodibenzothiazepine Lead Compound (SBI-0797750) as a Potent and Selective Inhibitor of Plasmodium falciparum and P. vivax Glucose 6-Phosphate Dehydrogenase 6-Phosphogluconolactonase","volume":"66","author":"Berneburg","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_113","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Ochoa, B., Mart\u00ednez-Rosas, V., Morales-Luna, L., Calder\u00f3n-Jaimes, E., Rocha-Ram\u00edrez, L.M., Ortega-Cuellar, D., Rufino-Gonz\u00e1lez, Y., Gonz\u00e1lez-Valdez, A., Arreguin-Espinosa, R., and Enr\u00edquez-Flores, S. (2022). Pyridyl Methylsulfinyl Benzimidazole Derivatives as Promising Agents against Giardia lamblia and Trichomonas vaginalis. Molecules, 27.","DOI":"10.3390\/molecules27248902"},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Morales-Luna, L., Hern\u00e1ndez-Ochoa, B., Mart\u00ednez-Rosas, V., Navarrete-V\u00e1zquez, G., Ortega-Cuellar, D., Rufino-Gonz\u00e1lez, Y., Gonz\u00e1lez-Valdez, A., Arreguin-Espinosa, R., Franco-V\u00e1squez, A.M., and de la Cruz, V.P. (2022). Giardia lamblia G6PD::6PGL Fused Protein Inhibitors Decrease Trophozoite Viability: A New Alternative against Giardiasis. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232214358"},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Rosas, V., Hern\u00e1ndez-Ochoa, B., Navarrete-V\u00e1zquez, G., Mart\u00ednez-Conde, C., G\u00f3mez-Ch\u00e1vez, F., Morales-Luna, L., Gonz\u00e1lez-Valdez, A., Arreguin-Espinosa, R., Enr\u00edquez-Flores, S., and de la Cruz, V.P. (2022). Kinetic and Molecular Docking Studies to Determine the Effect of Inhibitors on the Activity and Structure of Fused G6PD::6PGL Protein from Trichomonas vaginalis. Molecules, 27.","DOI":"10.3390\/molecules27041174"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"21","DOI":"10.2174\/1573409911666150414145937","article-title":"Antiprotozoal Nitazoxanide Derivatives: Synthesis, Bioassays and QSAR Study Combined with Docking for Mechanistic Insight","volume":"11","author":"Scior","year":"2015","journal-title":"Curr. Comput. Aided-Drug Des."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"3490","DOI":"10.1016\/j.bmcl.2017.05.071","article-title":"Synthesis, in vitro and in vivo giardicidal activity of nitrothiazole-NSAID chimeras displaying broad antiprotozoal spectrum","volume":"27","author":"Scior","year":"2017","journal-title":"Bioorganic Med. Chem. Lett."},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"Hern\u00e1ndez-Ochoa, B., G\u00f3mez-Manzo, S., S\u00e1nchez-Carrillo, A., Marcial-Quino, J., Rocha-Ram\u00edrez, L.M., Santos-Segura, A., Ram\u00edrez-Nava, E.J., Arreguin-Espinosa, R., Cuevas-Cruz, M., and M\u00e9ndez-Tenorio, A. (2020). Enhanced Antigiardial Effect of Omeprazole Analog Benzimidazole Compounds. 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