{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:06:18Z","timestamp":1793156778625,"version":"4.5.0"},"reference-count":327,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,7]],"date-time":"2024-01-07T00:00:00Z","timestamp":1704585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE014711"],"award-info":[{"award-number":["DE014711"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE019455"],"award-info":[{"award-number":["DE019455"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE023810"],"award-info":[{"award-number":["DE023810"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE020102"],"award-info":[{"award-number":["DE020102"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE026186"],"award-info":[{"award-number":["DE026186"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Dental caries is a significant oral and public health problem worldwide, especially in low-income populations. The risk of dental caries increases with frequent intake of dietary carbohydrates, including sugars, leading to increased acidity and disruption of the symbiotic diverse and complex microbial community of health. Excess acid production leads to a dysbiotic shift in the bacterial biofilm composition, demineralization of tooth structure, and cavities. Highly acidic and acid-tolerant species associated with caries include Streptococcus mutans, Lactobacillus, Actinomyces, Bifidobacterium, and Scardovia species. The differences in microbiotas depend on tooth site, extent of carious lesions, and rate of disease progression. Metagenomics and metatranscriptomics not only reveal the structure and genetic potential of the caries-associated microbiome, but, more importantly, capture the genetic makeup of the metabolically active microbiome in lesion sites. Due to its multifactorial nature, caries has been difficult to prevent. The use of topical fluoride has had a significant impact on reducing caries in clinical settings, but the approach is costly; the results are less sustainable for high-caries-risk individuals, especially children. Developing treatment regimens that specifically target S.\u00a0mutans and other acidogenic bacteria, such as using nanoparticles, show promise in altering the cariogenic microbiome, thereby combatting the disease.<\/jats:p>","DOI":"10.3390\/microorganisms12010121","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T03:49:04Z","timestamp":1704685744000},"page":"121","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":308,"title":["The Evolving Microbiome of Dental Caries"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2043-4853","authenticated-orcid":false,"given":"Grace","family":"Spatafora","sequence":"first","affiliation":[{"name":"Biology and Program in Molecular Biology and Biochemistry, Middlebury College, Middlebury, VT 05753, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5476-1943","authenticated-orcid":false,"given":"Yihong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Public and Ecosystem Health, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuesong","family":"He","sequence":"additional","affiliation":[{"name":"ADA-Forsyth Institute, Cambridge, MA 02142, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5458-0843","authenticated-orcid":false,"given":"Annie","family":"Cowan","sequence":"additional","affiliation":[{"name":"The Mayo Clinic College of Medicine and Science, Rochester, MN 55905, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1331-9163","authenticated-orcid":false,"given":"Anne C. 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Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"GBD 2017 Oral Disorders Collaborators, Bernabe, E., Marcenes, W., Hernandez, C.R., Bailey, J., Abreu, L.G., Alipour, V., Amini, S., Arabloo, J., and Arefi, Z. (2020). Global, regional, and national levels and trends in burden of oral conditions from 1990 to 2017: A systematic analysis for the global burden of disease 2017 study. J. Dent. Res., 99, 362\u2013373.","DOI":"10.1177\/0022034520908533"},{"key":"ref_5","unstructured":"Agency for Healthcare Research and Quality (2018). MEPS HC-188B: 2016 Dental Visits, Agency for Healthcare Research and Quality."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Fantom, N., and Serajuddin, U. (2016). The World Bank\u2019s Classification of Countries by Income, The World Bank. Policy Research Working Paper 7528.","DOI":"10.1596\/1813-9450-7528"},{"key":"ref_7","first-page":"189","article-title":"The new definition of oral health and relationship between oral health and quality of life","volume":"20","author":"Hescot","year":"2017","journal-title":"Chin. J. Dent. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1177\/0022034517693566","article-title":"Global, regional, and national prevalence, incidence, and disability-adjusted life years for oral conditions for 195 countries, 1990\u20132015: A systematic analysis for the global burden of diseases, injuries, and risk factors","volume":"96","author":"Kassebaum","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_9","unstructured":"Centers for Disease Control and Prevention (2019). Oral Health Surveillance Report: Trends in Dental Caries and Sealants, Tooth Retention, and Edentulism, United States, 1999\u20132004 to 2011\u20132016, Centers for Disease Control and Prevention, US Dept of Health and Human Services."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"S94","DOI":"10.1111\/jcpe.12677","article-title":"Global epidemiology of dental caries and severe periodontitis\u2014A comprehensive review","volume":"44","author":"Frencken","year":"2017","journal-title":"J. Clin. Periodontol."},{"key":"ref_11","unstructured":"Benzian, H., and Williams, D. (2015). The Challenge of Oral Disease\u2014A Call for Global Action. The Oral Health Atlas, FDI World Dental Federation. [2nd ed.]."},{"key":"ref_12","unstructured":"U.S. Department of Health & Human Services (1999). Ten great public health achievements\u2013United States, 1900\u20131999. MMWR Morb. Mortal. Wkly. Rep., 48, 241\u2013243."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kim, H.N., Kong, W.S., Lee, J.H., and Kim, J.B. (2019). Reduction of dental caries among children and adolescents from a 15-year community water fluoridation program in a township area, Korea. Int. J. Environ. Res. Public Health, 16.","DOI":"10.3390\/ijerph16071306"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1001\/jamapediatrics.2020.2201","article-title":"Association between community water fluoridation and severe dental caries experience in 4-year-old New Zealand children","volume":"174","author":"Schluter","year":"2020","journal-title":"JAMA Pediatr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1111\/cdoe.12697","article-title":"Water fluoridation, dental caries and parental ratings of child oral health","volume":"50","author":"Foley","year":"2021","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/sj.bdj.2017.836","article-title":"Why are caries levels reducing in five-year-olds in England?","volume":"223","author":"Davies","year":"2017","journal-title":"Br. Dent. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/cdoe.12526","article-title":"Changes in dental caries and sugar intake before and during the conflict in Libya: A natural experiment","volume":"48","author":"Arheiam","year":"2020","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_18","first-page":"49","article-title":"Increased dental use may affect changes in treated and untreated dental caries in young children","volume":"4","author":"Lin","year":"2019","journal-title":"JDR Clin. Trans. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1016\/j.adaj.2017.04.013","article-title":"Trends in dental caries in children and adolescents according to poverty status in the United States from 1999 through 2004 and from 2011 through 2014","volume":"148","author":"Dye","year":"2017","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_20","unstructured":"Dye, B.A., Tan, S., Smith, V., Lewis, B.G., Barker, L.K., Thornton-Evans, G., Eke, P.I., Beltran-Aguilar, E.D., Horowitz, A.M., and Li, C.H. (2007). Trends in Oral Health Status: United States, 1988\u20131994 and 1999\u20132004, National Center for Health Statistics. Vital and Health Statistics."},{"key":"ref_21","unstructured":"Dye, B.A., Thornton-Evans, G., Li, X., and Iafolla, T. (2015). Dental Caries and Tooth Loss in Adults in the United States, 2011\u20132012, US Department of Health and Human Services, Centers for Disease Control and Prevention, National Center for Health Statistics. NCHS Data Brief."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1007\/s40496-015-0064-9","article-title":"Declining caries trends: Are we satisfied?","volume":"2","author":"Lagerweij","year":"2015","journal-title":"Curr. Oral Health Rep."},{"key":"ref_23","unstructured":"World Health Organization (2022). Global Oral Health Status Report: Towards Universal Health Coverage for Oral Health by 2030, World Health Organization."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"115","DOI":"10.2105\/AJPH.2014.301869","article-title":"Age, period and cohort trends in caries of permanent teeth in four developed countries","volume":"104","author":"Bernabe","year":"2014","journal-title":"Am. J. Public Health"},{"key":"ref_25","first-page":"113","article-title":"Promoting oral health and quality of life of older people\u2014The need for public health action","volume":"16","author":"Petersen","year":"2018","journal-title":"Oral Health Prev. Dent."},{"key":"ref_26","unstructured":"Fleming, E., and Afful, J. (2018). Prevalence of Total and Untreated Dental Caries among Youth: United States, 2015\u20132016, US Department of Health and Human Services, Centers for Disease Control and Prevention, National Center for Health Statistics. NCHS Data Brief."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Gupta, N., Vujicic, M., Yarbrough, C., and Harrison, B. (2018). Disparities in untreated caries among children and adults in the U.S., 2011\u20132014. BMC Oral Health, 18.","DOI":"10.1186\/s12903-018-0493-7"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1177\/0022034519866628","article-title":"Modifiable factors explain socioeconomic inequalities in children\u2019s dental caries","volume":"98","author":"Peres","year":"2019","journal-title":"J. Dent. Res."},{"key":"ref_29","first-page":"714","article-title":"Clinical protocols for caries management by risk assessment","volume":"35","author":"Jenson","year":"2007","journal-title":"J. Calif. Dent. Assoc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1111\/j.1600-0528.2005.00233.x","article-title":"Cariogram\u2014A multifactorial risk assessment model for a multifactorial disease","volume":"33","author":"Bratthall","year":"2005","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_31","unstructured":"World Health Organization (2013). Oral Health Surveys: Basic Methods, World Health Organization. [5th ed.]."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1590\/S1806-83242012000700016","article-title":"Epidemiology of dental caries: When structure and context matter","volume":"26","author":"Frazao","year":"2012","journal-title":"Braz. Oral Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.prosdent.2013.07.014","article-title":"Dental caries: An updated medical model of risk assessment","volume":"111","author":"Kutsch","year":"2014","journal-title":"J. Prosthet. Dent."},{"key":"ref_34","first-page":"193","article-title":"\u201cICDAS\u201d\u2014An international system for caries detection and assessment being developed to facilitate caries epidemiology, research and appropriate clinical management","volume":"21","author":"Pitts","year":"2004","journal-title":"Community Dent. Health"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1111\/j.1600-0528.2007.00347.x","article-title":"The International Caries Detection and Assessment System (ICDAS): An integrated system for measuring dental caries","volume":"35","author":"Ismail","year":"2007","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e41","DOI":"10.1111\/cdoe.12025","article-title":"International Caries Detection and Assessment System (ICDAS) and its International Caries Classification and Management System (ICCMS)\u2014Methods for staging of the caries process and enabling dentists to manage caries","volume":"41","author":"Pitts","year":"2013","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/ng.2536","article-title":"Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions","volume":"45","author":"Adler","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"881S","DOI":"10.1093\/ajcn\/78.4.881S","article-title":"Sugars and dental caries","volume":"78","year":"2003","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_39","first-page":"249","article-title":"Dental caries in Norwegian children during and after the last World War; a preliminary report","volume":"42","author":"Toverud","year":"1949","journal-title":"Proc. R. Soc. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1001\/archpedi.1948.02030020810002","article-title":"Analysis of wartime reduction of dental caries in European children; with special regard to observations in Norway","volume":"75","author":"Sognnaes","year":"1948","journal-title":"Am. J. Dis. Child."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1177\/00220345010800090201","article-title":"The Vipeholm Dental Caries Study: Recollections and reflections 50 years later","volume":"80","author":"Krasse","year":"2001","journal-title":"J. Dent. Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"American Academy of Pediatrics, Committee on Native American Child Health, Canadian Paediatric Society, and First Nations, Inuit and M\u00e9tis Committee (2011). Early childhood caries in indigenous communities. Pediatrics, 127, 1190\u20131198.","DOI":"10.1542\/peds.2011-0847"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"S296","DOI":"10.4103\/0975-7406.155963","article-title":"Role of dietary habits and diet in caries occurrence and severity among urban adolescent school children","volume":"7","author":"Punitha","year":"2015","journal-title":"J. Pharm. Bioallied Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1177\/00220345440230040401","article-title":"Intra-oral hydrogen-ion concentrations associated with dental caries activity","volume":"23","author":"Stephan","year":"1944","journal-title":"J. Dent. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"7945","DOI":"10.1128\/JB.00858-07","article-title":"The EPS matrix: The \u201chouse of biofilm cells\u201d","volume":"189","author":"Flemming","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_46","first-page":"71","article-title":"Risk factors for dental caries in young children: A systematic review of the literature","volume":"21","author":"Harris","year":"2004","journal-title":"Community Dent. Health"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1159\/000358333","article-title":"Caries: Review of human genetics research","volume":"48","author":"Vieira","year":"2014","journal-title":"Caries Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1159\/000314676","article-title":"Genes and their effects on dental caries may differ between primary and permanent dentitions","volume":"44","author":"Wang","year":"2010","journal-title":"Caries Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.archoralbio.2016.05.009","article-title":"Antimicrobial peptides in saliva of children with severe early childhood caries","volume":"69","author":"Colombo","year":"2016","journal-title":"Arch. Oral Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1080\/19490976.2016.1162363","article-title":"The microbiome of the oral mucosa in irritable bowel syndrome","volume":"7","author":"Fourie","year":"2016","journal-title":"Gut Microbes"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/sj.bdj.2016.865","article-title":"The oral microbiome\u2014An update for oral healthcare professionals","volume":"221","author":"Kilian","year":"2016","journal-title":"Br. Dent. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1159\/000508328","article-title":"Gut microbiota dysbiosis drives the development of colorectal cancer","volume":"102","author":"Fan","year":"2021","journal-title":"Digestion"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"572","DOI":"10.4161\/gmic.21772","article-title":"The microbiota link to irritable bowel syndrome: An emerging story","volume":"3","author":"Jeffery","year":"2012","journal-title":"Gut Microbes"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1126\/science.1124234","article-title":"Metagenomic analysis of the human distal gut microbiome","volume":"312","author":"Gill","year":"2006","journal-title":"Science"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Kianoush, N., Adler, C.J., Nguyen, K.A., Browne, G.V., Simonian, M., and Hunter, N. (2014). Bacterial profile of dentine caries and the impact of pH on bacterial population diversity. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0092940"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Black, G.V. (1884). The Formation of Poisons by Micro-Organisms. A Biological Study of the Germ Theory of Disease, P Blakiston, Son & Co.","DOI":"10.5962\/bhl.title.21164"},{"key":"ref_57","unstructured":"Miller, W.D. (1890). The Micro-Organisms of the Human Mouth. The Local and General Diseas Which Are Caused by Them, The S. S. White Dental MFG. Co."},{"key":"ref_58","first-page":"481","article-title":"A study of the microorganisms of dental caries","volume":"36","author":"Howe","year":"1917","journal-title":"J. Med. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1128\/jb.24.4.321-334.1932","article-title":"Acid production and tolerance of lactobacilli from dental caries and other sources as measured by the glass electrode","volume":"24","author":"Rosebury","year":"1932","journal-title":"J. Bacteriol."},{"key":"ref_60","first-page":"141","article-title":"On the bacterial factor in the etiology of dental caries","volume":"5","author":"Clarke","year":"1924","journal-title":"Br. J. Exp. Pathol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1177\/00220345540330020201","article-title":"Use of the germfree animal technic in the study of experimental dental caries. I. Basic observations on rats reared free of all microorganisms","volume":"33","author":"Orland","year":"1954","journal-title":"J. Dent. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/0003-9969(60)90091-1","article-title":"The infectious and transmissible nature of experimental dental caries. Findings and implications","volume":"1","author":"Keyes","year":"1960","journal-title":"Arch. Oral Biol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"9","DOI":"10.14219\/jada.archive.1960.0138","article-title":"Demonstration of the etiologic role of streptococci in experimental caries in the hamster","volume":"61","author":"Fitzgerald","year":"1960","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/0003-9969(68)90142-8","article-title":"Characteristics of caries-inducing human streptococci resembling Streptococcus mutans","volume":"13","author":"Edwardsson","year":"1968","journal-title":"Arch. Oral Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1128\/iai.11.6.1252-1260.1975","article-title":"Association of Streptococcus mutans with human dental decay","volume":"11","author":"Loesche","year":"1975","journal-title":"Infect. Immun."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/0003-9969(73)90076-9","article-title":"Changes in Streptococcus mutans and lactobacilli in plaque in relation to the initiation of dental caries in Negro children","volume":"18","author":"Ikeda","year":"1973","journal-title":"Arch. Oral Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1128\/iai.9.4.624-630.1974","article-title":"Relationship between the concentration of bacteria in saliva and the colonization of teeth in humans","volume":"9","author":"Green","year":"1974","journal-title":"Infect. Immun."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1834-7819.1971.tb02310.x","article-title":"A study of caries aetiology in New South Wales schoolchildren. I. The streptococcal flora of plaque and caries prevalence","volume":"16","author":"Schamschula","year":"1971","journal-title":"Aust. Dent. J."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Marsh, P.D. (2006). Dental plaque as a biofilm and a microbial community\u2014Implications for health and disease. BMC Oral Health, 6.","DOI":"10.1186\/1472-6831-6-S1-S14"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1177\/00220345890680090101","article-title":"Effects of carbohydrate pulses and pH on population shifts within oral microbial communities in vitro","volume":"68","author":"Bradshaw","year":"1989","journal-title":"J. Dent. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.imlet.2014.08.017","article-title":"The oral microbiome and the immunobiology of periodontal disease and caries","volume":"162","author":"Costalonga","year":"2014","journal-title":"Immunol. Lett."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1177\/0022034510379602","article-title":"The role of bacteria in the caries process: Ecological perspectives","volume":"90","author":"Takahashi","year":"2011","journal-title":"J. Dent. Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1159\/000447309","article-title":"Ecological hypothesis of dentin and root caries","volume":"50","author":"Takahashi","year":"2016","journal-title":"Caries Res."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Kolenbrander, P.E., Palmer, R.J., Periasamy, S., and Jakubovics, N.S. (2010). Oral multispecies biofilm development and the key role of cell\u2013cell distance. Nat. Rev. Microbiol., 8.","DOI":"10.1038\/nrmicro2381"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"38882","DOI":"10.1038\/srep38882","article-title":"Salivary and pellicle proteome: A datamining analysis","volume":"6","author":"Schweigel","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.jds.2020.10.007","article-title":"Acquired salivary pellicle and oral diseases: A literature review","volume":"16","author":"Chawhuaveang","year":"2021","journal-title":"J. Dent. Sci."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/prd.12098","article-title":"Influence of saliva on the oral microbiota","volume":"70","author":"Marsh","year":"2016","journal-title":"Periodontology 2000"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"107S","DOI":"10.1016\/0003-9969(90)90139-2","article-title":"Role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces","volume":"35","author":"Gibbons","year":"1990","journal-title":"Arch. Oral Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1099\/mic.0.000010","article-title":"Streptococcus gordonii comCDE (competence) operon modulates biofilm formation with Candida albicans","volume":"161","author":"Jack","year":"2015","journal-title":"Microbiology"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"399","DOI":"10.7883\/yoken.JJID.2016.492","article-title":"Contribution of Streptococcus gordonii Hsa Adhesin to Biofilm Formation","volume":"70","author":"Oguchi","year":"2017","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.colsurfb.2014.05.020","article-title":"What interactions drive the salivary mucosal pellicle formation?","volume":"120","author":"Gibbins","year":"2014","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Nadell, C.D., Xavier, J.B., Levin, S.A., and Foster, K.R. (2008). The evolution of quorum sensing in bacterial biofilms. PLoS Biol., 6.","DOI":"10.1371\/journal.pbio.0060014"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/omi.12207","article-title":"ArcR modulates biofilm formation in the dental plaque colonizer Streptococcus gordonii","volume":"33","author":"Robinson","year":"2018","journal-title":"Mol. Oral Microbiol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.tim.2017.09.008","article-title":"Oral biofilms: Pathogens, matrix, and polymicrobial interactions in microenvironments","volume":"26","author":"Bowen","year":"2018","journal-title":"Trends Microbiol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1002\/pro.2671","article-title":"Structure of amylase-binding protein A of Streptococcus gordonii: A potential receptor for human salivary alpha-amylase enzyme","volume":"24","author":"Sethi","year":"2015","journal-title":"Protein Sci."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"435","DOI":"10.4161\/viru.2.5.16140","article-title":"Bacterial interactions in dental biofilm","volume":"2","author":"Huang","year":"2011","journal-title":"Virulence"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Haase, E.M., Kou, Y., Sabharwal, A., Liao, Y.C., Lan, T., Lindqvist, C., and Scannapieco, F.A. (2017). Comparative genomics and evolution of the amylase-binding proteins of oral streptococci. BMC Microbiol., 17.","DOI":"10.1186\/s12866-017-1005-7"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2000.tb09393.x","article-title":"Alkali production by oral bacteria and protection against dental caries","volume":"193","author":"Burne","year":"2000","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/omi.12044","article-title":"The effect of arginine on oral biofilm communities","volume":"29","author":"Nascimento","year":"2014","journal-title":"Mol. Oral Microbiol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1128\/AEM.03887-15","article-title":"Characterization of a highly arginolytic Streptococcus species that potently antagonizes Streptococcus mutans","volume":"82","author":"Huang","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.anaerobe.2013.04.015","article-title":"Identification of Veillonella tobetsuensis in tongue biofilm by using a species-specific primer pair","volume":"22","author":"Mashima","year":"2013","journal-title":"Anaerobe"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1128\/JB.02512-14","article-title":"The interaction between Streptococcus spp. and Veillonella tobetsuensis in the early stages of oral biofilm formation","volume":"197","author":"Mashima","year":"2015","journal-title":"J. Bacteriol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1902\/jop.1976.47.1.1","article-title":"Structure of the microbial flora associated with periodontal health and disease in man. A light and electron microscopic study","volume":"47","author":"Listgarten","year":"1976","journal-title":"J. Periodontol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1128\/MMBR.66.3.486-505.2002","article-title":"Communication among oral bacteria","volume":"66","author":"Kolenbrander","year":"2002","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"290","DOI":"10.3390\/nu2030290","article-title":"Streptococcus mutans, caries and simulation models","volume":"2","author":"Forssten","year":"2010","journal-title":"Nutrients"},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Moye, Z.D., Zeng, L., and Burne, R.A. (2014). Fueling the caries process: Carbohydrate metabolism and gene regulation by Streptococcus mutans. J. Oral Microbiol., 6.","DOI":"10.3402\/jom.v6.24878"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.1016\/j.jmb.2015.09.022","article-title":"Intermicrobial interactions as a driver for community composition and stratification of oral biofilms","volume":"427","author":"Jakubovics","year":"2015","journal-title":"J. Mol. Biol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/omi.12042","article-title":"Genetic basis of coaggregation receptor polysaccharide biosynthesis in Streptococcus sanguinis and related species","volume":"29","author":"Yang","year":"2014","journal-title":"Mol. Oral Microbiol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.tim.2014.10.010","article-title":"Solving the etiology of dental caries","volume":"23","author":"Mira","year":"2015","journal-title":"Trends Microbiol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1159\/000259844","article-title":"The predominant cultivable flora of carious plaque and carious dentine","volume":"7","author":"Loesche","year":"1973","journal-title":"Caries Res."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1159\/000073390","article-title":"The acid-tolerant microbiota associated with plaque from initial caries and healthy tooth surfaces","volume":"37","author":"Svensater","year":"2003","journal-title":"Caries Res."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1159\/000047482","article-title":"The predominant microflora of nursing caries lesions","volume":"35","author":"Marchant","year":"2001","journal-title":"Caries Res."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/j.1600-0765.1977.tb00113.x","article-title":"Attempts to increase viable count recovery of human supragingival dental plaque","volume":"12","author":"Manganiello","year":"1977","journal-title":"J. Periodontal Res."},{"key":"ref_104","doi-asserted-by":"crossref","unstructured":"Human Microbiome Project Consortium (2012). Structure, function and diversity of the healthy human microbiome. Nature, 486, 207\u2013214.","DOI":"10.1038\/nature11234"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J. Bacteriol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1128\/JCM.40.3.1001-1009.2002","article-title":"Molecular analysis of bacterial species associated with childhood caries","volume":"40","author":"Becker","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"5753","DOI":"10.1128\/JCM.43.11.5753-5759.2005","article-title":"Microbial risk indicators of early childhood caries","volume":"43","author":"Corby","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"5721","DOI":"10.1128\/JCM.43.11.5721-5732.2005","article-title":"Defining the normal bacterial flora of the oral cavity","volume":"43","author":"Aas","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1128\/JCM.01622-06","article-title":"Genetic profiling of the oral microbiota associated with severe early-childhood caries","volume":"45","author":"Li","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Gross, E.L., Beall, C.J., Kutsch, S.R., Firestone, N.D., Leys, E.J., and Griffen, A.L. (2012). Beyond Streptococcus mutans: Dental caries onset linked to multiple species by 16S rRNA community analysis. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0047722"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.1128\/JCM.42.7.3023-3029.2004","article-title":"Molecular analysis of the microflora associated with dental caries","volume":"42","author":"Munson","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1159\/000320158","article-title":"Clonal analysis of the microbiota of severe early childhood caries","volume":"44","author":"Kanasi","year":"2010","journal-title":"Caries Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1128\/JCM.02427-10","article-title":"Cultivable anaerobic microbiota of severe early childhood caries","volume":"49","author":"Tanner","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1177\/0022034517736496","article-title":"The caries microbiome: Implications for reversing dysbiosis","volume":"29","author":"Tanner","year":"2018","journal-title":"Adv. Dent. Res."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Schulze-Schweifing, K., Banerjee, A., and Wade, W.G. (2014). Comparison of bacterial culture and 16S rRNA community profiling by clonal analysis and pyrosequencing for the characterization of the dentine caries-associated microbiome. Front. Cell. Infect. Microbiol., 4.","DOI":"10.3389\/fcimb.2014.00164"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1016\/j.bbadis.2014.06.015","article-title":"Next generation sequencing technology: Advances and applications","volume":"1842","author":"Buermans","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"219","DOI":"10.3389\/fgene.2015.00219","article-title":"From next-generation sequencing to systematic modeling of the gut microbiome","volume":"6","author":"Ji","year":"2015","journal-title":"Front. Genet."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.mimet.2016.02.016","article-title":"Next-generation sequencing (NGS) in the microbiological world: How to make the most of your money","volume":"138","author":"Vincent","year":"2017","journal-title":"J. Microbiol. Methods"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"C90","DOI":"10.1177\/00220345770560032401","article-title":"A longitudinal epidemiological study on dental plaque and the development of dental caries\u2013interim results after two years","volume":"56","author":"Hardie","year":"1977","journal-title":"J. Dent. Res."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1177\/00220345910700120501","article-title":"In vitro acidogenic potential and mutans streptococci of human smooth-surface plaque associated with initial caries lesions and sound enamel","volume":"70","author":"Sansone","year":"1991","journal-title":"J. Dent. Res."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1177\/00220345890680120301","article-title":"The microflora associated with the development of initial enamel decalcification below orthodontic bands in vivo in children living in a fluoridated-water area","volume":"68","author":"Boyar","year":"1989","journal-title":"J. Dent. Res."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1177\/0022034512455031","article-title":"White-spot lesions and gingivitis microbiotas in orthodontic patients","volume":"91","author":"Tanner","year":"2012","journal-title":"J. Dent. Res."},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Torlakovic, L., Klepac-Ceraj, V., Ogaard, B., Cotton, S.L., Paster, B.J., and Olsen, I. (2012). Microbial community succession on developing lesions on human enamel. J. Oral Microbiol., 4.","DOI":"10.3402\/jom.v4i0.16125"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1128\/JCM.01410-07","article-title":"Bacteria of dental caries in primary and permanent teeth in children and young adults","volume":"46","author":"Aas","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"12375","DOI":"10.1073\/pnas.1919099117","article-title":"Spatial mapping of polymicrobial communities reveals a precise biogeography associated with human dental caries","volume":"117","author":"Kim","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1128\/iai.11.4.727-731.1975","article-title":"Predominant cultivable flora isolated from human root surface caries plaque","volume":"11","author":"Syed","year":"1975","journal-title":"Infect. Immun."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1177\/00220345960750040201","article-title":"The final pH of bacteria comprising the predominant flora on sound and carious human root and enamel surfaces","volume":"75","author":"Lopman","year":"1996","journal-title":"J. Dent. Res."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1177\/00220345010800091101","article-title":"The predominant aciduric microflora of root-caries lesions","volume":"80","author":"Brailsford","year":"2001","journal-title":"J. Dent. Res."},{"key":"ref_129","first-page":"283","article-title":"Association of salivary Streptococcus mutans levels determined by rapid detection system using monoclonal antibodies with prevalence of root surface caries","volume":"21","author":"Ikebe","year":"2008","journal-title":"Am. J. Dent."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/j.1399-302X.2008.00470.x","article-title":"Association between Bifidobacteriaceae and the clinical severity of root caries lesions","volume":"24","author":"Mantzourani","year":"2009","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_131","first-page":"295","article-title":"Profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria","volume":"24","author":"Hashimoto","year":"2011","journal-title":"Am. J. Dent."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1034\/j.1601-0825.2002.01796.x","article-title":"Bacterial and yeast flora of root surface caries in elderly, ethnic Chinese","volume":"8","author":"Shen","year":"2002","journal-title":"Oral Dis."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10096-008-0662-8","article-title":"Microarray analysis of the microflora of root caries in elderly","volume":"28","author":"Preza","year":"2009","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis. Off. Publ. Eur. Soc. Clin. Microbiol."},{"key":"ref_134","doi-asserted-by":"crossref","unstructured":"Chen, L., Qin, B., Du, M., Zhong, H., Xu, Q., Li, Y., Zhang, P., and Fan, M. (2015). Extensive description and comparison of human supra-gingival microbiome in root caries and health. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0117064"},{"key":"ref_135","doi-asserted-by":"crossref","unstructured":"Dame-Teixeira, N., Parolo, C.C., Maltz, M., Tugnait, A., Devine, D., and Do, T. (2016). Actinomyces spp. gene expression in root caries lesions. J. Oral Microbiol., 8.","DOI":"10.3402\/jom.v8.32383"},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1159\/000322299","article-title":"Microbiota of dentinal caries as assessed by reverse-capture checkerboard analysis","volume":"45","author":"Lima","year":"2011","journal-title":"Caries Res."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1177\/0022034520913248","article-title":"Structural and functional characteristics of the microbiome in deep-dentin caries","volume":"99","author":"Liu","year":"2020","journal-title":"J. Dent. Res."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1128\/JCM.43.2.843-849.2005","article-title":"Molecular analysis of microbial diversity in advanced caries","volume":"43","author":"Chhour","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.jdent.2011.12.013","article-title":"Intraoral pH measurement of carious lesions with qPCR of cariogenic bacteria to differentiate caries activity","volume":"40","author":"Kuribayashi","year":"2012","journal-title":"J. Dent."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s00784-013-1009-0","article-title":"pH gradient and distribution of streptococci, lactobacilli, prevotellae, and fusobacteria in carious dentine","volume":"18","author":"Kianoush","year":"2014","journal-title":"Clin. Oral Investig."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1159\/000479304","article-title":"Root surface biofilms and caries","volume":"26","author":"Do","year":"2017","journal-title":"Monogr. Oral Sci."},{"key":"ref_142","doi-asserted-by":"crossref","unstructured":"Kressirer, C.A., Chen, T., Lake Harriman, K., Frias-Lopez, J., Dewhirst, F.E., Tavares, M.A., and Tanner, A.C. (2018). Functional profiles of coronal and dentin caries in children. J. Oral Microbiol., 10.","DOI":"10.1080\/20002297.2018.1495976"},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1159\/000260858","article-title":"The microflora associated with developing lesions of nursing caries","volume":"19","author":"Milnes","year":"1985","journal-title":"Caries Res."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/0003-9969(75)90042-4","article-title":"Similarity of bacteriocins of Streptococcus mutans from mother and infant","volume":"20","author":"Berkowitz","year":"1975","journal-title":"Arch. Oral Biol."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1177\/00220345950740020901","article-title":"The fidelity of initial acquisition of mutans streptococci by infants from their mothers","volume":"74","author":"Li","year":"1995","journal-title":"J. Dent. Res."},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1034\/j.1600-0722.2003.00068.x","article-title":"Longitudinal study of intrafamilial mutans streptococci ribotypes","volume":"111","author":"Kohler","year":"2003","journal-title":"Eur. J. Oral Sci."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1177\/0022034511421201","article-title":"Microbiota of severe early childhood caries before and after therapy","volume":"90","author":"Tanner","year":"2011","journal-title":"J. Dent. Res."},{"key":"ref_148","first-page":"e16","article-title":"Aciduric microbiota and mutans streptococci in severe and recurrent severe early childhood caries","volume":"34","author":"Hughes","year":"2012","journal-title":"Pediatr. Dent."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/cdoe.12200","article-title":"Factors associated with dental caries in a group of American Indian children at age 36 months","volume":"44","author":"Warren","year":"2016","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/jphd.12061","article-title":"Oral health status in Navajo Nation Head Start children","volume":"74","author":"Batliner","year":"2014","journal-title":"J. Public Health Dent."},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1177\/0022034517718819","article-title":"Microbiome associated with severe caries in Canadian First Nations children","volume":"96","author":"Agnello","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1002\/cre2.94","article-title":"Genotypic characterization and comparison of Streptococcus mutans in American Indian and Southeast Iowa children","volume":"3","author":"Villhauer","year":"2017","journal-title":"Clin. Exp. Dent. Res."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1177\/0022034515609554","article-title":"The microbiome in populations with a low and high prevalence of caries","volume":"95","author":"Johansson","year":"2016","journal-title":"J. Dent. Res."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1177\/0022034517731758","article-title":"Microbial complexes and caries in 17-year-olds with and without Streptococcus mutans","volume":"97","author":"Eriksson","year":"2018","journal-title":"J. Dent. Res."},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1159\/000339482","article-title":"Shifts in the microbial population in relation to in situ caries progression","volume":"46","author":"Thomas","year":"2012","journal-title":"Caries Res."},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"1968","DOI":"10.1128\/IAI.00087-14","article-title":"Symbiotic relationship between Streptococcus mutans and Candida albicans synergizes virulence of plaque biofilms in vivo","volume":"82","author":"Falsetta","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1159\/000481833","article-title":"Candida albicans and early childhood caries: A systematic review and meta-analysis","volume":"52","author":"Xiao","year":"2018","journal-title":"Caries Res."},{"key":"ref_158","doi-asserted-by":"crossref","unstructured":"Janus, M.M., Crielaard, W., Volgenant, C.M., van der Veen, M.H., Brandt, B.W., and Krom, B.P. (2017). Candida albicans alters the bacterial microbiome of early in vitro oral biofilms. J. Oral Microbiol., 9.","DOI":"10.1080\/20002297.2016.1270613"},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1128\/IAI.05896-11","article-title":"Synergistic interaction between Candida albicans and commensal oral streptococci in a novel in vitro mucosal model","volume":"80","author":"Diaz","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_160","doi-asserted-by":"crossref","unstructured":"Metwalli, K.H., Khan, S.A., Krom, B.P., and Jabra-Rizk, M.A. (2013). Streptococcus mutans, Candida albicans, and the human mouth: A sticky situation. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003616"},{"key":"ref_161","doi-asserted-by":"crossref","unstructured":"He, J., Kim, D., Zhou, X., Ahn, S.J., Burne, R.A., Richards, V.P., and Koo, H. (2017). RNA-Seq reveals enhanced sugar metabolism in Streptococcus mutans co-cultured with Candida albicans within mixed-species biofilms. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.01036"},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1007\/s40368-015-0180-1","article-title":"First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth","volume":"16","author":"Kneist","year":"2015","journal-title":"Eur. Arch. Paediatr. Dent."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.1007\/s00784-015-1696-9","article-title":"Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota-a clinical study","volume":"20","author":"Auschill","year":"2016","journal-title":"Clin. Oral Investig."},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1177\/0022034518790941","article-title":"Association between oral candida and bacteriome in children with severe ECC","volume":"97","author":"Xiao","year":"2018","journal-title":"J. Dent. Res."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1177\/00220345940730031301","article-title":"Role of micro-organisms in caries etiology","volume":"73","year":"1994","journal-title":"J. Dent. Res."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"80S","DOI":"10.1046\/j.1365-2672.83.s1.9.x","article-title":"Sugar metabolism by mutans streptococci","volume":"83","author":"Colby","year":"1997","journal-title":"J. Appl. Microbiol."},{"key":"ref_167","first-page":"269","article-title":"Comparative genomics of oral isolates of Streptococcus mutans by in silico genome subtraction does not reveal accessory DNA associated with severe early childhood caries","volume":"21C","author":"Argimon","year":"2014","journal-title":"Infect. Genet. Evol. J. Mol. Epidemiol. Evol. Genet. Infect. Dis."},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1128\/JCM.01993-10","article-title":"Distribution of putative virulence genes in Streptococcus mutans strains does not correlate with caries experience","volume":"49","author":"Argimon","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_169","doi-asserted-by":"crossref","unstructured":"Palmer, S.R., Miller, J.H., Abranches, J., Zeng, L., Lefebure, T., Richards, V.P., Lemos, J.A., Stanhope, M.J., and Burne, R.A. (2013). Phenotypic heterogeneity of genomically-diverse isolates of Streptococcus mutans. PLoS ONE, 8.","DOI":"10.1371\/annotation\/ffff8cd5-b8fa-4d3c-a993-e5169198f1e6"},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.ebiom.2017.09.027","article-title":"Streptococcus mutans adhesin biotypes that match and predict individual caries development","volume":"24","author":"Esberg","year":"2017","journal-title":"EBioMedicine"},{"key":"ref_171","first-page":"11","article-title":"Cnm is a major virulence factor of invasive Streptococcus mutans and part of a conserved three-gene locus","volume":"29","author":"Miller","year":"2014","journal-title":"Mol. Oral Microbiol."},{"key":"ref_172","doi-asserted-by":"crossref","unstructured":"Rainey, K., Michalek, S.M., Wen, Z.T., and Wu, H. (2019). Glycosyltransferase mediated biofilm matrix dynamics and virulence of Streptococcus mutans. Appl. Environ. Microbiol., 85.","DOI":"10.1128\/AEM.02247-18"},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1177\/154411130201300202","article-title":"A mixed-bacteria ecological approach to understanding the role of the oral bacteria in dental caries causation: An alternative to Streptococcus mutans and the specific-plaque hypothesis","volume":"13","author":"Kleinberg","year":"2002","journal-title":"Crit. Rev. Oral Biol. Med."},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/j.1600-0528.1997.tb00902.x","article-title":"Does assessment of microbial composition of plaque\/saliva allow for diagnosis of disease activity of individuals?","volume":"25","author":"Bowden","year":"1997","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1051\/lait:2003019","article-title":"Isolation, characterisation and identification of lactobacilli focusing mainly on cheeses and other dairy products","volume":"83","author":"Coeuret","year":"2003","journal-title":"Lait"},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.anaerobe.2011.04.001","article-title":"Adherence capacities of oral lactobacilli for potential probiotic purposes","volume":"17","author":"Samot","year":"2011","journal-title":"Anaerobe"},{"key":"ref_177","unstructured":"Ljungh, A., and Wadstrom, T. (2009). Lactobacillus Molecular Biology from Genomics to Probiotics, Caister Academic Press."},{"key":"ref_178","doi-asserted-by":"crossref","first-page":"38","DOI":"10.2174\/1874285800802010038","article-title":"Ecology of lactobacilli in the oral cavity: A review of literature","volume":"2","author":"Badet","year":"2008","journal-title":"Open Microbiol. J."},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1159\/000337353","article-title":"Mutans streptococci and lactobacilli colonization in predentate children from the neonatal period to seven months of age","volume":"46","author":"Plonka","year":"2012","journal-title":"Caries Res."},{"key":"ref_180","first-page":"401","article-title":"Oral microflora in infants delivered vaginally and by caesarean section","volume":"21","author":"Magnusson","year":"2011","journal-title":"Int. J. Paediatr. Dent.\/Br. Paedodontic Soc. Int. Assoc. Dent. Child."},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1097\/MPG.0b013e31826f2bc6","article-title":"Oral microbial profile discriminates breast-fed from formula-fed infants","volume":"56","author":"Holgerson","year":"2013","journal-title":"J. Pediatr. Gastroenterol. Nutr."},{"key":"ref_182","first-page":"e24","article-title":"Vertical transmission of mutans streptococci and lactobacilli in Thai families","volume":"34","author":"Teanpaisan","year":"2012","journal-title":"Pediatr. Dent."},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1111\/j.1875-595X.2007.tb00148.x","article-title":"Longitudinal study of the presence of mutans streptococci and lactobacilli in relation to dental caries development in 3\u201324 month old Thai children","volume":"57","author":"Teanpaisan","year":"2007","journal-title":"Int. Dent. J."},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1128\/iai.46.3.765-772.1984","article-title":"Longitudinal investigation of bacteriology of human fissure decay: Epidemiological studies in molars shortly after eruption","volume":"46","author":"Loesche","year":"1984","journal-title":"Infect. Immun."},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1159\/000262081","article-title":"Lactobacilli, mutants streptococci and dental caries: A longitudinal study in 2-year-old children up to the age of 5 years","volume":"29","author":"Roeters","year":"1995","journal-title":"Caries Res."},{"key":"ref_186","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1177\/0022034509360010","article-title":"Microbial risk markers for childhood caries in pediatricians\u2019 offices","volume":"89","author":"Kanasi","year":"2010","journal-title":"J. Dent. Res."},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1159\/000096099","article-title":"Diversity of lactobacilli in the oral cavities of young women with dental caries","volume":"41","author":"Caufield","year":"2007","journal-title":"Caries Res."},{"key":"ref_188","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.mimet.2010.05.010","article-title":"Determining the genetic diversity of lactobacilli from the oral cavity","volume":"82","author":"Yang","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"ref_189","unstructured":"Argimon, S., Casey, S., Saraithong, P., Schon, C., Li, Y., and Caufield, P. (2014, January 27\u201330). Comparison of bacterial culture and culture-independent 16S rRNA gene sequencing for the study of Lactobacillus diversity in children with severe early childhood caries. Proceedings of the 5th ASM Conference on Beneficial Microbes, Washington, DC, USA."},{"key":"ref_190","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/BF03262741","article-title":"Diversity of Lactobacillus species in deep carious lesions of primary molars","volume":"11","author":"Kneist","year":"2010","journal-title":"Eur. Arch. Paediatr. Dent."},{"key":"ref_191","doi-asserted-by":"crossref","unstructured":"Rocas, I.N., Alves, F.R., Rachid, C.T., Lima, K.C., Assuncao, I.V., Gomes, P.N., and Siqueira, J.F. (2016). Microbiome of deep dentinal caries lesions in teeth with symptomatic irreversible pulpitis. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0154653"},{"key":"ref_192","doi-asserted-by":"crossref","first-page":"110S","DOI":"10.1177\/0022034515576052","article-title":"Oral lactobacilli and dental caries: A model for niche adaptation in humans","volume":"94","author":"Caufield","year":"2015","journal-title":"J. Dent. Res."},{"key":"ref_193","first-page":"93","article-title":"Pre- and post-treatment levels of salivary mutans streptococci and lactobacilli in pre-school children","volume":"9","author":"Twetman","year":"1999","journal-title":"Int. J. Paediatr. Dent.\/Br. Paedodontic Soc. Int. Assoc. Dent. Child."},{"key":"ref_194","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1159\/000351673","article-title":"Changes in Candida spp., mutans streptococci and lactobacilli following treatment of early childhood caries: A 1-year follow-up","volume":"48","author":"Klinke","year":"2014","journal-title":"Caries Res."},{"key":"ref_195","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.7754\/Clin.Lab.2013.121225","article-title":"Identification of lactobacilli from deep carious lesions by means of species-specific PCR and MALDI-TOF mass spectrometry","volume":"59","author":"Callaway","year":"2013","journal-title":"Clin. Lab."},{"key":"ref_196","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1177\/154405910808700421","article-title":"Periodontal pathogens and gestational diabetes mellitus","volume":"87","author":"Dasanayake","year":"2008","journal-title":"J. Dent. Res."},{"key":"ref_197","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0003-9969(84)90015-3","article-title":"Growth and acid tolerance of human dental plaque bacteria","volume":"29","author":"Harper","year":"1984","journal-title":"Arch. Oral Biol."},{"key":"ref_198","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1177\/00220345830620011601","article-title":"Oral colonization and pathogenicity of Actinomyces israelii in gnotobiotic rats","volume":"62","author":"Behbehani","year":"1983","journal-title":"J. Dent. Res."},{"key":"ref_199","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1177\/00220345800590072301","article-title":"Cariogenic potential of Bifidobacterium in gnotobiotic rats","volume":"59","year":"1980","journal-title":"J. Dent. Res."},{"key":"ref_200","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.job.2017.05.002","article-title":"Scardovia wiggsiae and its potential role as a caries pathogen","volume":"59","author":"Kressirer","year":"2017","journal-title":"J. Oral Biosci."},{"key":"ref_201","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1159\/000222659","article-title":"The isolation of bifidobacteria from occlusal carious lesions in children and adults","volume":"43","author":"Mantzourani","year":"2009","journal-title":"Caries Res."},{"key":"ref_202","first-page":"32","article-title":"Salivary levels of bifidobacteria in caries-free and caries-active children","volume":"23","author":"Kaur","year":"2013","journal-title":"Int. J. Paediatr. Dent.\/Br. Paedodontic Soc. Int. Assoc. Dent. Child."},{"key":"ref_203","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1007\/s00784-016-1958-1","article-title":"Acidogenicity of dual-species biofilms of bifidobacteria and Streptococcus mutans","volume":"21","author":"Brighenti","year":"2017","journal-title":"Clin. Oral Investig."},{"key":"ref_204","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/j.1469-0691.2012.03857.x","article-title":"Identifying a healthy oral microbiome through metagenomics","volume":"18","author":"Alcaraz","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_205","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1875-595X.2011.00025.x","article-title":"Dental caries pathogenicity: A genomic and metagenomic perspective","volume":"61","author":"Peterson","year":"2011","journal-title":"Int. Dent. J."},{"key":"ref_206","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1093\/gbe\/evp057","article-title":"Comparative metagenomics and population dynamics of the gut microbiota in mother and infant","volume":"2","author":"Vaishampayan","year":"2010","journal-title":"Genome Biol. Evol."},{"key":"ref_207","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/2042-5783-2-3","article-title":"Metagenomics\u2014A guide from sampling to data analysis","volume":"2","author":"Thomas","year":"2012","journal-title":"Microb. Inform. Exp."},{"key":"ref_208","doi-asserted-by":"crossref","first-page":"803","DOI":"10.3748\/wjg.v21.i3.803","article-title":"Application of metagenomics in the human gut microbiome","volume":"21","author":"Wang","year":"2015","journal-title":"World J. Gastroenterol."},{"key":"ref_209","doi-asserted-by":"crossref","unstructured":"Jovel, J., Patterson, J., Wang, W., Hotte, N., O\u2019Keefe, S., Mitchel, T., Perry, T., Kao, D., Mason, A.L., and Madsen, K.L. (2016). Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00459"},{"key":"ref_210","doi-asserted-by":"crossref","first-page":"424","DOI":"10.4161\/viru.28532","article-title":"Application of metagenomics in understanding oral health and disease","volume":"5","author":"Xu","year":"2014","journal-title":"Virulence"},{"key":"ref_211","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1038\/srep01843","article-title":"Metagenomic sequencing reveals microbiota and its functional potential associated with periodontal disease","volume":"3","author":"Wang","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_212","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1111\/j.2041-1014.2010.00587.x","article-title":"Community and gene composition of a human dental plaque microbiota obtained by metagenomic sequencing","volume":"25","author":"Xie","year":"2010","journal-title":"Mol. Oral Microbiol."},{"key":"ref_213","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/ismej.2011.85","article-title":"The oral metagenome in health and disease","volume":"6","author":"Alcaraz","year":"2012","journal-title":"ISME J."},{"key":"ref_214","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1034\/j.1399-302X.1999.140108.x","article-title":"Effects of pH on the glucose and lactate metabolisms by the washed cells of Actinomyces naeslundii under anaerobic and aerobic conditions","volume":"14","author":"Takahashi","year":"1999","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_215","first-page":"95","article-title":"Responses of cariogenic streptococci to environmental stresses","volume":"7","author":"Lemos","year":"2005","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_216","doi-asserted-by":"crossref","first-page":"e01012","DOI":"10.1128\/mBio.01012-14","article-title":"Metatranscriptomics of the human oral microbiome during health and disease","volume":"5","author":"Jorth","year":"2014","journal-title":"mBio"},{"key":"ref_217","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1177\/0022034515606045","article-title":"Oral microbiome metabolism: From \u201cWho Are They?\u201d to \u201cWhat Are They Doing?\u201d","volume":"94","author":"Takahashi","year":"2015","journal-title":"J. Dent. Res."},{"key":"ref_218","doi-asserted-by":"crossref","unstructured":"Simon-Soro, A., Guillen-Navarro, M., and Mira, A. (2014). Metatranscriptomics reveals overall active bacterial composition in caries lesions. J. Oral Microbiol., 6.","DOI":"10.3402\/jom.v6.25443"},{"key":"ref_219","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/nrmicro2873","article-title":"The keystone-pathogen hypothesis","volume":"10","author":"Hajishengallis","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_220","doi-asserted-by":"crossref","unstructured":"Peterson, S.N., Meissner, T., Su, A.I., Snesrud, E., Ong, A.C., Schork, N.J., and Bretz, W.A. (2014). Functional expression of dental plaque microbiota. Front. Cell. Infect. Microbiol., 4.","DOI":"10.3389\/fcimb.2014.00108"},{"key":"ref_221","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1093\/bioinformatics\/btv526","article-title":"metaModules identifies key functional subnetworks in microbiome-related disease","volume":"32","author":"May","year":"2016","journal-title":"Bioinformatics"},{"key":"ref_222","doi-asserted-by":"crossref","unstructured":"Belstrom, D., Constancias, F., Liu, Y., Yang, L., Drautz-Moses, D.I., Schuster, S.C., Kohli, G.S., Jakobsen, T.H., Holmstrup, P., and Givskov, M. (2017). Metagenomic and metatranscriptomic analysis of saliva reveals disease-associated microbiota in patients with periodontitis and dental caries. NPJ Biofilms Microbiomes, 3.","DOI":"10.1038\/s41522-017-0031-4"},{"key":"ref_223","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.micinf.2015.03.014","article-title":"Beyond microbial community composition: Functional activities of the oral microbiome in health and disease","volume":"17","year":"2015","journal-title":"Microbes Infect."},{"key":"ref_224","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1002\/pmic.201100503","article-title":"Deep metaproteomic analysis of human salivary supernatant","volume":"12","author":"Jagtap","year":"2012","journal-title":"Proteomics"},{"key":"ref_225","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1177\/0022034510377792","article-title":"Metabolomics of supragingival plaque and oral bacteria","volume":"89","author":"Takahashi","year":"2010","journal-title":"J. Dent. Res."},{"key":"ref_226","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1038\/ismej.2015.72","article-title":"Meta-omics uncover temporal regulation of pathways across oral microbiome genera during in vitro sugar metabolism","volume":"9","author":"Edlund","year":"2015","journal-title":"ISME J."},{"key":"ref_227","doi-asserted-by":"crossref","unstructured":"Edlund, A., Garg, N., Mohimani, H., Gurevich, A., He, X., Shi, W., Dorrestein, P.C., and McLean, J.S. (2017). Metabolic fingerprints from the human oral microbiome reveal a vast knowledge gap of secreted small peptidic molecules. mSystems, 2.","DOI":"10.1128\/mSystems.00058-17"},{"key":"ref_228","doi-asserted-by":"crossref","first-page":"C142","DOI":"10.1177\/002203457705600308011","article-title":"The effect of a plaque control program on gingivitis and dental caries in schoolchildren","volume":"56","author":"Axelsson","year":"1977","journal-title":"J. Dent. Res."},{"key":"ref_229","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1177\/0022034517735295","article-title":"In sickness and in health-what does the oral microbiome mean to us? An ecological perspective","volume":"29","author":"Marsh","year":"2018","journal-title":"Adv. Dent. Res."},{"key":"ref_230","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1177\/00220345770560031101","article-title":"Reduction of dental decay in rampant caries individuals following short-term kanamycin treatment","volume":"56","author":"Loesche","year":"1977","journal-title":"J. Dent. Res."},{"key":"ref_231","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1177\/00220345740530010201","article-title":"Temporary suppression of Streptococcus mutans in humans through topical application of vancomycin","volume":"53","author":"Jordan","year":"1974","journal-title":"J. Dent. Res."},{"key":"ref_232","first-page":"226","article-title":"Effect of antimicrobial interventions on the oral microbiota associated with early childhood caries","volume":"37","author":"Li","year":"2015","journal-title":"Pediatr. Dent."},{"key":"ref_233","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1159\/000365296","article-title":"Characterization of the arginolytic microflora provides insights into pH homeostasis in human oral biofilms","volume":"49","author":"Huang","year":"2015","journal-title":"Caries Res."},{"key":"ref_234","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1007\/s00248-014-0535-x","article-title":"Stability and resilience of oral microcosms toward acidification and Candida outgrowth by arginine supplementation","volume":"69","author":"Koopman","year":"2015","journal-title":"Microb. Ecol."},{"key":"ref_235","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1159\/000329181","article-title":"Antimicrobial traits of tea- and cranberry-derived polyphenols against Streptococcus mutans","volume":"45","author":"Yoo","year":"2011","journal-title":"Caries Res."},{"key":"ref_236","doi-asserted-by":"crossref","unstructured":"de Sousa, D.L., Araujo Lima, R., Zanin, I.C., Klein, M.I., Janal, M.N., and Duarte, S. (2015). Effect of twice-daily blue light treatment on matrix-rich biofilm development. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0131941"},{"key":"ref_237","first-page":"246","article-title":"Evidence of Effectiveness of Current Therapies to Prevent and Treat Early Childhood Caries","volume":"37","author":"Twetman","year":"2015","journal-title":"Pediatr. Dent."},{"key":"ref_238","first-page":"69","article-title":"Fluoride and oral health","volume":"33","author":"Baez","year":"2016","journal-title":"Community Dent. Health"},{"key":"ref_239","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1177\/0022034518774331","article-title":"Water fluoridation and dental caries in U.S. children and adolescents","volume":"97","author":"Slade","year":"2018","journal-title":"J. Dent. Res."},{"key":"ref_240","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.annepidem.2017.05.014","article-title":"Science, politics, and communication: The case of community water fluoridation in the US","volume":"28","author":"Allukian","year":"2018","journal-title":"Ann. Epidemiol."},{"key":"ref_241","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1159\/000260422","article-title":"Effects of fluoride on the initial colonization of teeth in vivo","volume":"13","author":"Kilian","year":"1979","journal-title":"Caries Res."},{"key":"ref_242","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1159\/000264683","article-title":"Effect of various rinsing protocols after use of amine fluoride\/stannous fluoride toothpaste on the bacterial composition of dental plaque","volume":"43","author":"Gerardu","year":"2009","journal-title":"Caries Res."},{"key":"ref_243","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1159\/000260304","article-title":"Effects of fluoride on enzymatic regulation of bacterial carbohydrate metabolism","volume":"11","author":"Hamilton","year":"1977","journal-title":"Caries Res."},{"key":"ref_244","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1111\/j.1574-6976.2003.tb00627.x","article-title":"Fluoride and organic weak acids as modulators of microbial physiology","volume":"26","author":"Marquis","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_245","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1177\/00220345900690S128","article-title":"Biochemical effects of fluoride on oral bacteria","volume":"69","author":"Hamilton","year":"1990","journal-title":"J. Dent. Res."},{"key":"ref_246","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1177\/00220345900690S127","article-title":"Effects of fluoride on the microbial ecology of dental plaque","volume":"69","author":"Bowden","year":"1990","journal-title":"J. Dent. Res."},{"key":"ref_247","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1159\/000057864","article-title":"Effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms","volume":"36","author":"Bradshaw","year":"2002","journal-title":"Caries Res."},{"key":"ref_248","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1139\/w04-128","article-title":"Inhibitory effect of sodium fluoride and chlorhexidine on the growth of oral lactobacilli","volume":"51","author":"Wiese","year":"2005","journal-title":"Can. J. Microbiol."},{"key":"ref_249","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1399-302X.1990.tb00417.x","article-title":"Mutans streptococci in saliva and dental caries in children living in a high and a low fluoride area","volume":"5","author":"Twetman","year":"1990","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_250","first-page":"CD002284","article-title":"Fluoride mouthrinses for preventing dental caries in children and adolescents","volume":"7","author":"Marinho","year":"2016","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_251","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.archoralbio.2018.02.010","article-title":"Effect of sodium fluoride on oral biofilm microbiota and enamel demineralization","volume":"89","author":"Thurnheer","year":"2018","journal-title":"Arch. Oral Biol."},{"key":"ref_252","doi-asserted-by":"crossref","unstructured":"Koopman, J.E., van der Kaaij, N.C., Buijs, M.J., Elyassi, Y., van der Veen, M.H., Crielaard, W., Ten Cate, J.M., and Zaura, E. (2015). The effect of fixed orthodontic appliances and fluoride mouthwash on the oral microbiome of adolescents\u2014A randomized controlled clinical trial. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137318"},{"key":"ref_253","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.1111\/jam.12655","article-title":"Biofilm community diversity after exposure to 0.4% stannous fluoride gels","volume":"117","author":"Reilly","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_254","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/odi.12407","article-title":"Short-term effects of povidone iodine and sodium fluoride therapy on plaque levels and microbiome diversity","volume":"22","author":"Reilly","year":"2016","journal-title":"Oral Dis."},{"key":"ref_255","first-page":"CD002279","article-title":"Fluoride varnishes for preventing dental caries in children and adolescents","volume":"7","author":"Marinho","year":"2013","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_256","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1600-0528.2008.00427.x","article-title":"A 2-year community-randomized controlled trial of fluoride varnish to prevent early childhood caries in Aboriginal children","volume":"36","author":"Lawrence","year":"2008","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_257","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1007\/s10995-007-0294-0","article-title":"An observational study of the association of fluoride varnish applied during well child visits and the prevention of early childhood caries in American Indian children","volume":"12","author":"Holve","year":"2008","journal-title":"Matern. Child Health J."},{"key":"ref_258","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1177\/154405910608500211","article-title":"Fluoride varnish efficacy in preventing early childhood caries","volume":"85","author":"Weintraub","year":"2006","journal-title":"J. Dent. Res."},{"key":"ref_259","unstructured":"American Academy of Pediatric Dentistry (2016). Guideline on caries-risk assessment and management for infants, children, and adolescents. Pediatr. Dent., 38, 142\u2013149."},{"key":"ref_260","first-page":"399","article-title":"Effect of fluoride varnish and chlorhexidine-thymol varnish on mutans streptococci levels in human dental plaque: A double-blinded randomized controlled trial","volume":"24","author":"Paul","year":"2014","journal-title":"Int. J. Paediatr. Dent.\/Br. Paedodontic Soc. Int. Assoc. Dent. Child."},{"key":"ref_261","first-page":"291","article-title":"Caries outcome following an intensive fluoride varnish treatment regimen for children at high risk for early childhood caries","volume":"28","author":"Paek","year":"2018","journal-title":"Int. J. Paediatr. Dent.\/Br. Paedodontic Soc. Int. Assoc. Dent. Child."},{"key":"ref_262","first-page":"169","article-title":"Mother and youth access (MAYA) maternal chlorhexidine, counselling and paediatric fluoride varnish randomized clinical trial to prevent early childhood caries","volume":"22","author":"Gansky","year":"2012","journal-title":"Int. J. Paediatr. Dent.\/Br. Paedodontic Soc. Int. Assoc. Dent. Child."},{"key":"ref_263","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1111\/j.1399-302X.2008.00458.x","article-title":"Sodium fluoride and chlorhexidine effect in the inhibition of mutans streptococci in children with dental caries: A randomized, double-blind clinical trial","volume":"23","author":"Lobo","year":"2008","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_264","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1159\/000264686","article-title":"Randomized equivalence trial of intensive and semiannual applications of fluoride varnish in the primary dentition","volume":"43","author":"Weinstein","year":"2009","journal-title":"Caries Res."},{"key":"ref_265","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1128\/AAC.9.2.357","article-title":"Electrically generated silver ions: Quantitative effects on bacterial and mammalian cells","volume":"9","author":"Berger","year":"1976","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_266","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s12903-016-0171-6","article-title":"Caries remineralisation and arresting effect in children by professionally applied fluoride treatment\u2014A systematic review","volume":"16","author":"Gao","year":"2016","journal-title":"BMC Oral Health"},{"key":"ref_267","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.cden.2018.08.011","article-title":"Evidence-based dentistry update on silver diamine fluoride","volume":"63","author":"Crystal","year":"2019","journal-title":"Dent. Clin. N. Am."},{"key":"ref_268","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1177\/154405910508400807","article-title":"Efficacy of silver diamine fluoride for caries reduction in primary teeth and first permanent molars of schoolchildren: 36-month clinical trial","volume":"84","author":"Llodra","year":"2005","journal-title":"J. Dent. Res."},{"key":"ref_269","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1177\/0022034512452278","article-title":"Randomized trial on fluorides and sealants for fissure caries prevention","volume":"91","author":"Liu","year":"2012","journal-title":"J. Dent. Res."},{"key":"ref_270","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1177\/0022034510375825","article-title":"A randomized trial on root caries prevention in elders","volume":"89","author":"Tan","year":"2010","journal-title":"J. Dent. Res."},{"key":"ref_271","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1177\/0022034508329406","article-title":"Silver diamine fluoride: A caries \u201csilver-fluoride bullet\u201d","volume":"88","author":"Rosenblatt","year":"2009","journal-title":"J. Dent. Res."},{"key":"ref_272","first-page":"87","article-title":"Mechanism of antiplaque action of diamine silver fluoride","volume":"16","author":"Suzuki","year":"1976","journal-title":"J. Osaka Univ. Dent. Sch."},{"key":"ref_273","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/j.joen.2010.02.029","article-title":"Antimicrobial efficacy of 3.8% silver diamine fluoride and its effect on root dentin","volume":"36","author":"Hiraishi","year":"2010","journal-title":"J. Endod."},{"key":"ref_274","first-page":"155","article-title":"Inability to form a biofilm of Streptococcus mutans on silver fluoride- and potassium iodide-treated demineralized dentin","volume":"40","author":"Knight","year":"2009","journal-title":"Quintessence Int."},{"key":"ref_275","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1834-7819.2007.tb00460.x","article-title":"Differences between normal and demineralized dentine pretreated with silver fluoride and potassium iodide after an in vitro challenge by Streptococcus mutans","volume":"52","author":"Knight","year":"2007","journal-title":"Aust. Dent. J."},{"key":"ref_276","first-page":"2","article-title":"Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms","volume":"22","author":"Chu","year":"2012","journal-title":"Int. J. Paediatr. Dent.\/Br. Paedodontic Soc. Int. Assoc. Dent. Child."},{"key":"ref_277","doi-asserted-by":"crossref","unstructured":"Mei, M.L., Li, Q.L., Chu, C.H., Lo, E.C., and Samaranayake, L.P. (2013). Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries. Ann. Clin. Microbiol. Antimicrob., 12.","DOI":"10.1186\/1476-0711-12-4"},{"key":"ref_278","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/adj.12276","article-title":"Effect of silver diamine fluoride and potassium iodide on residual bacteria in dentinal tubules","volume":"60","author":"Hamama","year":"2015","journal-title":"Aust. Dent. J."},{"key":"ref_279","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jdent.2018.08.007","article-title":"Caries-arresting effects of silver diamine fluoride and sodium fluoride on dentine caries lesions","volume":"78","author":"Yu","year":"2018","journal-title":"J. Dent."},{"key":"ref_280","first-page":"CD002280","article-title":"Fluoride gels for preventing dental caries in children and adolescents","volume":"6","author":"Marinho","year":"2015","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_281","first-page":"CD007868","article-title":"Fluoride toothpastes of different concentrations for preventing dental caries in children and adolescents","volume":"1","author":"Walsh","year":"2010","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_282","doi-asserted-by":"crossref","first-page":"117","DOI":"10.5644\/ama2006-124.80","article-title":"Dental caries: Strategies to control this preventable disease","volume":"42","year":"2013","journal-title":"Acta Med. Acad."},{"key":"ref_283","first-page":"1","article-title":"Diammine silver fluoride and its clinical application","volume":"12","author":"Yamaga","year":"1972","journal-title":"J. Osaka Univ. Dent. Sch."},{"key":"ref_284","doi-asserted-by":"crossref","first-page":"233","DOI":"10.4103\/0972-0707.85796","article-title":"Remineralizing efficacy of silver diamine fluoride and glass ionomer type VII for their proposed use as indirect pulp capping materials\u2014Part II (A clinical study)","volume":"14","author":"Sinha","year":"2011","journal-title":"J. Conserv. Dent."},{"key":"ref_285","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1177\/0022034518774783","article-title":"Arresting dentine caries with silver diamine fluoride: What\u2019s behind it?","volume":"97","author":"Mei","year":"2018","journal-title":"J. Dent. Res."},{"key":"ref_286","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1159\/000496210","article-title":"ORIG Assessment of the silver penetration and distribution in carious lesions of deciduous teeth treated with silver diamine fluoride","volume":"53","author":"Li","year":"2019","journal-title":"Caries Res."},{"key":"ref_287","first-page":"135","article-title":"Use of silver diamine fluoride for dental caries management in children and adolescents, including those with special health care needs","volume":"39","author":"Crystal","year":"2017","journal-title":"Pediatr. Dent."},{"key":"ref_288","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/jphd.12241","article-title":"Effect and acceptance of silver diamine fluoride treatment on dental caries in primary teeth","volume":"78","author":"Clemens","year":"2018","journal-title":"J. Public Health Dent."},{"key":"ref_289","doi-asserted-by":"crossref","first-page":"185102","DOI":"10.1088\/0957-4484\/21\/18\/185102","article-title":"Development of nanostructured silver vanadates decorated with silver nanoparticles as a novel antibacterial agent","volume":"21","author":"Holtz","year":"2010","journal-title":"Nanotechnology"},{"key":"ref_290","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.nano.2008.04.005","article-title":"The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold","volume":"4","author":"Ruiz","year":"2008","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"ref_291","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1016\/j.jdent.2014.05.017","article-title":"A New \u201cSilver-Bullet\u201d to treat caries in children\u2014Nano Silver Fluoride: A randomised clinical trial","volume":"42","author":"Filho","year":"2014","journal-title":"J. Dent."},{"key":"ref_292","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.archoralbio.2016.03.002","article-title":"Evaluation of antibiofilm and mechanical properties of new nanocomposites based on acrylic resins and silver vanadate nanoparticles","volume":"67","author":"Valente","year":"2016","journal-title":"Arch. Oral Biol."},{"key":"ref_293","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.archoralbio.2018.06.005","article-title":"Analysis of the oral microbiome on the surface of modified dental polymers","volume":"93","author":"Alves","year":"2018","journal-title":"Arch. Oral Biol."},{"key":"ref_294","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/nature11234","article-title":"Structure, function and diversity of the healthy human microbiome","volume":"486","author":"Huttenhower","year":"2012","journal-title":"Nature"},{"key":"ref_295","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1146\/annurev-micro-091313-103456","article-title":"Effects of antibiotics on human microbiota and subsequent disease","volume":"68","author":"Keeney","year":"2014","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_296","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nri1857","article-title":"The scientific and public-health imperative for a vaccine against dental caries","volume":"6","author":"Taubman","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"ref_297","doi-asserted-by":"crossref","first-page":"7273","DOI":"10.1016\/j.vaccine.2017.11.007","article-title":"LT adjuvant modulates epitope specificity and improves the efficacy of murine antibodies elicited by sublingual vaccination with the N-terminal domain of Streptococcus mutans P1","volume":"35","author":"Batista","year":"2017","journal-title":"Vaccine"},{"key":"ref_298","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.imbio.2017.01.007","article-title":"Enhanced immune response to a dual-promoter anti-caries DNA vaccine orally delivered by attenuated Salmonella typhimurium","volume":"222","author":"Jiang","year":"2017","journal-title":"Immunobiology"},{"key":"ref_299","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s10719-017-9798-z","article-title":"Investigating the candidacy of the serotype specific rhamnan polysaccharide based glycoconjugates to prevent disease caused by the dental pathogen Streptococcus mutans","volume":"35","author":"Yang","year":"2018","journal-title":"Glycoconj. J."},{"key":"ref_300","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1159\/000084805","article-title":"Clinical trial of a plant-derived antibody on recolonization of mutans streptococci","volume":"39","author":"Weintraub","year":"2005","journal-title":"Caries Res."},{"key":"ref_301","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jbiotec.2017.01.002","article-title":"The use of bacteriophages to biocontrol oral biofilms","volume":"250","author":"Szafranski","year":"2017","journal-title":"J. Biotechnol."},{"key":"ref_302","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/microbiolspec.BAD-0006-2016","article-title":"Ecological therapeutic opportunities for oral diseases","volume":"5","author":"Hoare","year":"2017","journal-title":"Microbiol. Spectr."},{"key":"ref_303","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1159\/000484985","article-title":"Ecological approaches to dental caries prevention: Paradigm shift or shibboleth?","volume":"52","author":"Philip","year":"2018","journal-title":"Caries Res."},{"key":"ref_304","doi-asserted-by":"crossref","unstructured":"Lopez-Lopez, A., Camelo-Castillo, A., Ferrer, M.D., Simon-Soro, A., and Mira, A. (2017). Health-associated niche inhabitants as oral probiotics: The case of Streptococcus dentisani. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00379"},{"key":"ref_305","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1177\/0022034517735293","article-title":"Getting to know \u201cthe known unknowns\u201d: Heterogeneity in the oral microbiome","volume":"29","author":"Burne","year":"2018","journal-title":"Adv. Dent. Res."},{"key":"ref_306","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jdent.2016.03.002","article-title":"Probiotics for managing caries and periodontitis: Systematic review and meta-analysis","volume":"48","author":"Gruner","year":"2016","journal-title":"J. Dent."},{"key":"ref_307","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1177\/0022034517698096","article-title":"Targeting of Streptococcus mutans biofilms by a novel small molecule prevents dental caries and preserves the oral microbiome","volume":"96","author":"Garcia","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_308","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1128\/AAC.00919-15","article-title":"Molecule targeting glucosyltransferase inhibits Streptococcus mutans biofilm formation and virulence","volume":"60","author":"Ren","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_309","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.anaerobe.2017.05.016","article-title":"A novel antimicrobial peptide against dental-caries-associated bacteria","volume":"47","author":"Chen","year":"2017","journal-title":"Anaerobe"},{"key":"ref_310","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.peptides.2013.11.020","article-title":"Antimicrobial and anti-biofilm effect of Bac8c on major bacteria associated with dental caries and Streptococcus mutans biofilms","volume":"52","author":"Ding","year":"2014","journal-title":"Peptides"},{"key":"ref_311","doi-asserted-by":"crossref","unstructured":"Saputo, S., Faustoferri, R.C., and Quivey, R.G. (2018). Vitamin D compounds are bactericidal against Streptococcus mutans and target the bacitracin-associated efflux system. Antimicrob. Agents Chemother., 62.","DOI":"10.1128\/AAC.01675-17"},{"key":"ref_312","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1128\/AAC.50.4.1480-1488.2006","article-title":"Adding selectivity to antimicrobial peptides: Rational design of a multidomain peptide against Pseudomonas spp.","volume":"50","author":"Eckert","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_313","doi-asserted-by":"crossref","first-page":"3651","DOI":"10.1128\/AAC.00622-06","article-title":"Targeted killing of Streptococcus mutans by a pheromone-guided \u201csmart\u201d antimicrobial peptide","volume":"50","author":"Eckert","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_314","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1159\/000330510","article-title":"Clinical efficacy of a specifically targeted antimicrobial peptide mouth rinse: Targeted elimination of Streptococcus mutans and prevention of demineralization","volume":"45","author":"Sullivan","year":"2011","journal-title":"Caries Res."},{"key":"ref_315","doi-asserted-by":"crossref","first-page":"7569","DOI":"10.1073\/pnas.1506207112","article-title":"Precision-guided antimicrobial peptide as a targeted modulator of human microbial ecology","volume":"112","author":"Guo","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_316","doi-asserted-by":"crossref","first-page":"3446","DOI":"10.1128\/AAC.00342-11","article-title":"Selective membrane disruption: Mode of action of C16G2, a specifically targeted antimicrobial peptide","volume":"55","author":"Kaplan","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_317","doi-asserted-by":"crossref","first-page":"8566","DOI":"10.1021\/acsami.8b01089","article-title":"pH-sensitive compounds for selective inhibition of acid-producing bacteria","volume":"10","author":"Yang","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_318","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.1021\/nn507170s","article-title":"pH-activated nanoparticles for controlled topical delivery of farnesol to disrupt oral biofilm virulence","volume":"9","author":"Horev","year":"2015","journal-title":"ACS Nano"},{"key":"ref_319","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.biomaterials.2016.05.051","article-title":"Nanocatalysts promote Streptococcus mutans biofilm matrix degradation and enhance bacterial killing to suppress dental caries in vivo","volume":"101","author":"Gao","year":"2016","journal-title":"Biomaterials"},{"key":"ref_320","doi-asserted-by":"crossref","first-page":"9442","DOI":"10.1021\/acs.nanolett.1c02702","article-title":"Ferumoxytol nanoparticles target biofilms causing tooth decay in the human mouth","volume":"21","author":"Liu","year":"2021","journal-title":"Nano Lett."},{"key":"ref_321","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0140-6736(07)60031-2","article-title":"Dental caries","volume":"369","author":"Selwitz","year":"2007","journal-title":"Lancet"},{"key":"ref_322","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1177\/0022034515620779","article-title":"Predicting dental caries outcomes in children: A \u201crisky\u201d concept","volume":"95","author":"Divaris","year":"2016","journal-title":"J. Dent. Res."},{"key":"ref_323","first-page":"437","article-title":"Understanding caries from the oral microbiome perspective","volume":"44","author":"Tanner","year":"2016","journal-title":"J. Calif. Dent. Assoc."},{"key":"ref_324","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nrmicro.2016.94","article-title":"Biofilms: An emergent form of bacterial life","volume":"14","author":"Flemming","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_325","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1038\/nrmicro.2017.99","article-title":"Targeting microbial biofilms: Current and prospective therapeutic strategies","volume":"15","author":"Koo","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_326","doi-asserted-by":"crossref","first-page":"3497","DOI":"10.1002\/pmic.201400600","article-title":"The human oral metaproteome reveals potential biomarkers for caries disease","volume":"15","author":"Williamson","year":"2015","journal-title":"Proteomics"},{"key":"ref_327","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.chom.2015.08.005","article-title":"Prediction of early childhood caries via spatial-temporal variations of oral microbiota","volume":"18","author":"Teng","year":"2015","journal-title":"Cell Host Microbe"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/1\/121\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:41:43Z","timestamp":1760103703000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/1\/121"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,7]]},"references-count":327,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["microorganisms12010121"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12010121","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,7]]}}}