{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:38:26Z","timestamp":1760143106846,"version":"build-2065373602"},"reference-count":62,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovation, Republic of Serbia","award":["451-03-47\/2023-01\/200117","451-03-47\/2023-01\/200015","451-03-47\/2023-01\/200178"],"award-info":[{"award-number":["451-03-47\/2023-01\/200117","451-03-47\/2023-01\/200015","451-03-47\/2023-01\/200178"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The \u201cbeautiful viviparous fly\u201d, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank\u2122 under accession numbers MT027108\u2013MT027114) to those available in GenBank\u2122. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.<\/jats:p>","DOI":"10.3390\/microorganisms12020233","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T04:17:40Z","timestamp":1705983460000},"page":"233","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-5325","authenticated-orcid":false,"given":"Stanislav","family":"Simin","sequence":"first","affiliation":[{"name":"Faculty of Agriculture, Department of Veterinary Medicine, University of Novi Sad, Trg Dositeja Obradovi\u0107a 8, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8275-5885","authenticated-orcid":false,"given":"Sne\u017eana","family":"Tomanovi\u0107","sequence":"additional","affiliation":[{"name":"Group for Medical Entomology, Centre of Excellence for Food- and Vector-Borne Zoonoses, Institute for Medical Research\u2014National Institute of Republic of Serbia, University of Belgrade, 11129 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4778-5215","authenticated-orcid":false,"given":"Ratko","family":"Sukara","sequence":"additional","affiliation":[{"name":"Group for Medical Entomology, Centre of Excellence for Food- and Vector-Borne Zoonoses, Institute for Medical Research\u2014National Institute of Republic of Serbia, University of Belgrade, 11129 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marijana","family":"Stefanov","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Department of Veterinary Medicine, University of Novi Sad, Trg Dositeja Obradovi\u0107a 8, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Savovi\u0107","sequence":"additional","affiliation":[{"name":"Private Veterinary Practice \u201cMSV Medicus\u201d, Milice Stojadinovi\u0107 Srpkinje 1, 21209 Bukovac, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1262-7495","authenticated-orcid":false,"given":"Bojan","family":"Gaji\u0107","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4813-8370","authenticated-orcid":false,"given":"Vesna","family":"Lalo\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Department of Veterinary Medicine, University of Novi Sad, Trg Dositeja Obradovi\u0107a 8, 21000 Novi Sad, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,23]]},"reference":[{"key":"ref_1","first-page":"108","article-title":"Wohlfahrtia magnifica, Schin. and allied Russian species. The biology of this fly and its importance to man and domestic animals","volume":"11","author":"Portchinsky","year":"1916","journal-title":"Trans. Bur. Ent."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"James, M.T. (1947). 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