{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T23:54:53Z","timestamp":1770422093134,"version":"3.49.0"},"reference-count":41,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T00:00:00Z","timestamp":1706227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Cytomegalovirus (CMV), in addition to other agents, is part of the TORCH complex (Toxoplasma gondii, Rubella virus, Cytomegalovirus, Herpes simplex viruses, and other agents). CMV infection is the most frequent cause of congenital malformations. This study aimed to establish the variation of prevalence of anti-CMV antibodies in pregnant women from the South-West region of Romania, according to demographic factors, such as age and area of residence, in two separate time periods (2013\u20132016 and 2019\u20132022). We collected from the hospital records the age, place of residence, and anti-CMV antibody test results using immune electrochemiluminescence and chemiluminescence. This study found that the seroprevalence of anti-CMV IgM antibodies increased slightly from 2013\u20132016 to 2019\u20132022, from 1.92% to 2.26%, and for IgG antibodies from 93.68% to 94.96%. In both groups was observed a descending trend of anti-CMV IgM seroprevalence with an increase in age, showing a decrease in seroprevalence from 3.57% to 1.09% in pregnant women from rural areas in the 31\u201335 years age group, while in urban areas, we observed a decrease in seroprevalence from 11.11% to 3.06% in the &lt;20 years age group. The IgG seroprevalence showed an increase both in rural areas (from 93.97% to 95.52%) and urban areas (from 93.52% to 94.27%). In both groups, seroprevalence was higher in rural areas compared to urban regions. These results show a high rate of immunization against CMV in pregnant women in South-West Romania, which led to a low risk of acquiring the primary infection during pregnancy. However, the increase in the rate of primary CMV infections in pregnancy suggests the need for prioritizing screening programs and improving the existing protocols to enhance maternal and child healthcare.<\/jats:p>","DOI":"10.3390\/microorganisms12020268","type":"journal-article","created":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T10:54:27Z","timestamp":1706266467000},"page":"268","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Seroprevalence of Anti-Cytomegalovirus Antibodies in Pregnant Women from South-West Romania"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6731-6029","authenticated-orcid":false,"given":"Cristiana Luiza","family":"Radoi","sequence":"first","affiliation":[{"name":"Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3510-3838","authenticated-orcid":false,"given":"Ovidiu","family":"Zlatian","sequence":"additional","affiliation":[{"name":"Medical Laboratory, County Clinical Emergency Hospital of Craiova, 200349 Craiova, Romania"},{"name":"Microbiology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Balasoiu","sequence":"additional","affiliation":[{"name":"Medical Laboratory, County Clinical Emergency Hospital of Craiova, 200349 Craiova, Romania"},{"name":"Microbiology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiberiu-Liviu","family":"Dragomir","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, \u201cVictor Babes\u201d University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madalina Ioana","family":"Sorop","sequence":"additional","affiliation":[{"name":"Doctoral School, \u201cVictor Babes\u201d University of Medicine and Pharmacy, 300041 Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3099-8249","authenticated-orcid":false,"given":"Iulia Cristina","family":"Bagiu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, \u201cVictor Babes\u201d University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania"},{"name":"Multidisciplinary Research Center on Antimicrobial Resistance (MULTI-REZ), Microbiology Department, \u201cVictor Babes\u201d University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estera","family":"Boeriu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, \u201cVictor Babes\u201d University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6923-9146","authenticated-orcid":false,"given":"Monica","family":"Susan","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, Centre for Preventive Medicine,\u201dVictor Babes\u201d University of Medicine and Pharmacy, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogdan","family":"Sorop","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, \u201cVictor Babes\u201d University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Licinia Andrada","family":"Oprisoni","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Discipline of Pediatric Oncology and Hematology, \u201cVictor Babes\u201d University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominic Gabriel","family":"Iliescu","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Department, County Clinical Emergency Hospital of Craiova, 200349 Craiova, Romania"},{"name":"Obstetrics and Gynecology Department, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,26]]},"reference":[{"key":"ref_1","unstructured":"Duke, E.R., Boeckh, M., and Geballe, A.P. (2022). Encyclopedia of Gastroenterology, Elsevier. [2nd ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/rmv.535","article-title":"Review and Meta-Analysis of the Epidemiology of Congenital Cytomegalovirus (CMV) Infection","volume":"17","author":"Kenneson","year":"2007","journal-title":"Rev. Med. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/CMR.00062-12","article-title":"The \u201cSilent\u201d Global Burden of Congenital Cytomegalovirus","volume":"26","author":"Manicklal","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e11","DOI":"10.1093\/cid\/ciq085","article-title":"Attribution of Congenital Cytomegalovirus Infection to Primary versus Non-Primary Maternal Infection","volume":"52","author":"Wang","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/ajo.12408","article-title":"Congenital Cytomegalovirus Infection in Pregnancy: A Review of Prevalence, Clinical Features, Diagnosis and Prevention","volume":"56","author":"Naing","year":"2016","journal-title":"Aust. N. Z. J. Obstet. Gynaecol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Marcu, E.A., Dinescu, S.N., P\u0103dureanu, V., Dumitrescu, F., and Diaconu, R. (2022). Perinatal Exposure to HIV Infection: The Experience of Craiova Regional Centre, Romania. Healthcare, 10.","DOI":"10.3390\/healthcare10020308"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Cambrea, S.C., Marcu, E.A., Cucli, E., Badiu, D., Penciu, R., Petcu, C.L., Dumea, E., Halichidis, S., Pazara, L., and Mihai, C.M. (2022). Clinical and Biological Risk Factors Associated with Increased Mother-to-Child Transmission of HIV in Two South-East HIV-AIDS Regional Centers in Romania. Medicina, 58.","DOI":"10.3390\/medicina58020275"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/tme.12437","article-title":"Transfusion-Transmitted CMV Infection\u2014Current Knowledge and Future Perspectives","volume":"27","author":"Ziemann","year":"2017","journal-title":"Transfus. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Iijima, S. (2022). Pitfalls in the Serological Evaluation of Maternal Cytomegalovirus Infection as a Potential Cause of Fetal and Neonatal Involvements: A Narrative Literature Review. J. Clin. Med., 11.","DOI":"10.3390\/jcm11175006"},{"key":"ref_10","first-page":"75","article-title":"Clinical-Statistical Study of the Patients Who Suffer from Infections Which Are Brought about Factors of Torch Complex","volume":"8","author":"Neamtu","year":"2006","journal-title":"Craiova Medicala"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Mocanu, A., Gorun, F., Ciohat, I., Navolan, D., Malita, D., Vilibic-Cavlek, T., Dahma, G., Neamtu, R., Popescu, D., and Cioca, A. (2021). Simultaneous Seroprevalence to Toxoplasma gondii, Cytomegalovirus and Rubella Virus in Childbearing Women from Western Romania. Medicina, 57.","DOI":"10.3390\/medicina57090927"},{"key":"ref_12","first-page":"2439","article-title":"Cytomegalovirus Seroprevalence in Pregnant Women in the Western Region of Romania: A Large-Scale Study","volume":"20","author":"Gorun","year":"2020","journal-title":"Exp. Ther. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1017\/S0950268817000103","article-title":"Seroprevalence of Cytomegalovirus Infection in France in 2010","volume":"145","author":"Antona","year":"2017","journal-title":"Epidemiol. Infect."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"425","DOI":"10.5604\/17331331.1227668","article-title":"High Seroprevalence of CMV Among Women of Childbearing Age Implicates High Burden of Congenital Cytomegalovirus Infection in Poland","volume":"65","author":"Siennicka","year":"2017","journal-title":"Pol. J. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1002\/jmv.23757","article-title":"Susceptibility to Cytomegalovirus, Parvovirus B19 and Age-Dependent Differences in Levels of Rubella Antibodies among Pregnant Women","volume":"86","author":"Barlinn","year":"2014","journal-title":"J. Med. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s00508-016-1069-7","article-title":"Seroepidemiology of Cytomegalovirus Infections in Croatia","volume":"129","author":"Kolaric","year":"2017","journal-title":"Wien Klin. Wochenschr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1002\/bdra.23601","article-title":"Cytomegalovirus Infection in Pregnancy","volume":"109","author":"Davis","year":"2017","journal-title":"Birth Defects Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7437","DOI":"10.1016\/j.vaccine.2018.02.089","article-title":"Vaccination against the Human Cytomegalovirus","volume":"37","author":"Plotkin","year":"2019","journal-title":"Vaccine"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.tim.2005.02.009","article-title":"Insights into Viral Transmission at the Uterine-Placental Interface","volume":"13","author":"Pereira","year":"2005","journal-title":"Trends Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Revello, M.G., Tibaldi, C., Masuelli, G., Frisina, V., Sacchi, A., Furione, M., Arossa, A., Spinillo, A., Klersy, C., and Ceccarelli, M. (2015). Prevention of Primary Cytomegalovirus Infection in Pregnancy. EBioMedicine, 2.","DOI":"10.1016\/j.ebiom.2015.08.003"},{"key":"ref_21","unstructured":"National Institute of Public Health of Romania (2023). Informative Report of the State of Health 2019\u20132023, National Institute of Public Health of Romania."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"919","DOI":"10.4155\/bio.09.80","article-title":"Electrochemiluminescence in Bioanalysis","volume":"1","author":"Rhyne","year":"2009","journal-title":"Bioanalysis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1002\/jcla.1860060409","article-title":"Enzyme-Linked Immunoadsorbent Assay for the Detection of Cytomegalovirus-IgM: Comparison between Eight Commercial Kits, Immunofluorescence, and Immunoblotting","volume":"6","author":"Lazzarotto","year":"1992","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_24","first-page":"162","article-title":"Teenage Mothers, an Increasing Social Phenomenon in Romania. Causes, Consequences and Solutions","volume":"51","author":"Diaconescu","year":"2015","journal-title":"Rev. Cercet. Interv. Soc."},{"key":"ref_25","first-page":"55","article-title":"An 8 Years Analysis of Pregnancies and Births among Teenagers in a University Hospital in North-Eastern Romania","volume":"54","author":"Iorga","year":"2016","journal-title":"Rev. Cercet. Interv. Soc."},{"key":"ref_26","unstructured":"World Health Organisation (2023, February 17). Adolescent Pregnancy. Available online: http:\/\/www.who.int\/media-centre\/factsheets\/fs364\/en\/."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Fowler, K., Mucha, J., Neumann, M., Lewandowski, W., Kaczanowska, M., Grys, M., Schmidt, E., Natenshon, A., Talarico, C., and Buck, P.O. (2022). A Systematic Literature Review of the Global Seroprevalence of Cytomegalovirus: Possible Implications for Treatment, Screening, and Vaccine Development. BMC Public Health, 22.","DOI":"10.1186\/s12889-022-13971-7"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/CVI.05228-11","article-title":"National Prevalence Estimates for Cytomegalovirus IgM and IgG Avidity and Association between High IgM Antibody Titer and Low IgG Avidity","volume":"18","author":"Dollard","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.jiph.2013.08.006","article-title":"Cytomegalovirus Seroprevalence in Women with Bad Obstetric History in Kirkuk, Iraq","volume":"7","author":"Aljumaili","year":"2014","journal-title":"J. Infect. Public Health"},{"key":"ref_30","unstructured":"European Economic and Social Committee (2015). The Economic and Social Situation in Romania."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e23716","DOI":"10.1002\/jcla.23716","article-title":"Seroprevalence of CMV IgG and IgM in Korean Women of Childbearing Age","volume":"35","author":"Choi","year":"2021","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"427","DOI":"10.4314\/ejhs.v26i5.4","article-title":"Seroepidemiology Study of Cytomegalovirus and Rubella among Pregnant Women at St. Paul\u2019s Hospital Millennium Medical College, Addis Ababa, Ethiopia","volume":"26","author":"Yeshwondm","year":"2016","journal-title":"Ethiop. J. Health Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"45","DOI":"10.5897\/JMLD2013.0075","article-title":"Seroprevalence of Cytomegalovirus Infection among Pregnant Women at Omdurman Maternity Hospital, Sudan","volume":"4","author":"Khairi","year":"2013","journal-title":"J. Med. Lab. Diagn."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1128\/CVI.00203-06","article-title":"National Serosurvey of Cytomegalovirus in Australia","volume":"13","author":"Seale","year":"2006","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1128\/JCM.43.9.4713-4718.2005","article-title":"Diagnosis of and Screening for Cytomegalovirus Infection in Pregnant Women","volume":"43","author":"Munro","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s00430-012-0232-7","article-title":"Cytomegalovirus (CMV) Seroprevalence in Pregnant Women, Bone Marrow Donors and Adolescents in Germany, 1996\u20132010","volume":"201","author":"Enders","year":"2012","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1007\/s00103-003-0602-9","article-title":"Zytomegalievirus- (CMV-) Durchseuchung Und Hufigkeit von CMV-Primrinfektionen Bei Schwangeren Frauen in Deutschland","volume":"5","author":"Enders","year":"2003","journal-title":"Bundesgesundheitsblatt. Gesundheitsforschung. Gesundheitsschutz."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1055\/s-2008-1026231","article-title":"[Incidence of Congenital Infections]","volume":"51","author":"Friese","year":"1991","journal-title":"Geburtshilfe Frauenheilkd."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00430-009-0136-3","article-title":"Epidemiology of Human Cytomegalovirus (HCMV) in an Urban Region of Germany: What Has Changed?","volume":"199","author":"Doerr","year":"2010","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Wizman, S., Lamarre, V., Coic, L., Kakkar, F., Le Meur, J.B., Rousseau, C., Boucher, M., and Tapiero, B. (2016). Awareness of Cytomegalovirus and Risk Factors for Susceptibility among Pregnant Women, in Montreal, Canada. BMC Pregnancy Childbirth, 16.","DOI":"10.1186\/s12884-016-0844-9"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1007\/s10096-014-2170-3","article-title":"Impact of Socioeconomic Risk Factors on the Seroprevalence of Cytomegalovirus Infections in a Cohort of Pregnant Polish Women between 2010 and 2011","volume":"33","author":"Wujcicka","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/2\/268\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:50:02Z","timestamp":1760104202000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/2\/268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,26]]},"references-count":41,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["microorganisms12020268"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12020268","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,26]]}}}