{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T12:37:37Z","timestamp":1784810257835,"version":"3.55.0"},"reference-count":24,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T00:00:00Z","timestamp":1706572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82102414"],"award-info":[{"award-number":["82102414"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["pumch201911291"],"award-info":[{"award-number":["pumch201911291"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2021-I2M-1-038"],"award-info":[{"award-number":["2021-I2M-1-038"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Young Scientific Research Fund of PUMCH","award":["82102414"],"award-info":[{"award-number":["82102414"]}]},{"name":"Young Scientific Research Fund of PUMCH","award":["pumch201911291"],"award-info":[{"award-number":["pumch201911291"]}]},{"name":"Young Scientific Research Fund of PUMCH","award":["2021-I2M-1-038"],"award-info":[{"award-number":["2021-I2M-1-038"]}]},{"name":"Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences (CIFMS)","award":["82102414"],"award-info":[{"award-number":["82102414"]}]},{"name":"Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences (CIFMS)","award":["pumch201911291"],"award-info":[{"award-number":["pumch201911291"]}]},{"name":"Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Sciences (CIFMS)","award":["2021-I2M-1-038"],"award-info":[{"award-number":["2021-I2M-1-038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Moraxella catarrhalis, a commensal in the human nasopharynx, plays a significant role in the acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Its pathogenicity involves adherence to respiratory epithelial cells, leading to infection through a macropinocytosis-like mechanism. Previous investigations highlighted the diverse abilities of M. catarrhalis isolates with different phenotypes to adhere to and invade respiratory epithelial cells. This study used a murine COPD model and in vitro experiments to explore the factors influencing the pathogenicity of distinct phenotypes of M. catarrhalis. Transcriptome sequencing suggested a potential association between actin cytoskeleton regulation and the infection of lung epithelial cells by M. catarrhalis with different phenotypes. Electron microscopy and Western blot analyses revealed a decrease in filamentous actin (F-actin) expression upon infection with various M. catarrhalis phenotypes. Inhibition of actin polymerization indicated the involvement of F-actin dynamics in M. catarrhalis internalization, distinguishing it from the adhesion process. Notably, hindering F-actin polymerization impaired the internalization of M. catarrhalis. These findings contribute vital theoretical insights for developing preventive strategies and individualized clinical treatments for AECOPD patients infected with M. catarrhalis. The study underscores the importance of understanding the nuanced interactions between M. catarrhalis phenotypes and host lung epithelial cells, offering valuable implications for the management of AECOPD infections.<\/jats:p>","DOI":"10.3390\/microorganisms12020291","type":"journal-article","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T03:45:12Z","timestamp":1706586312000},"page":"291","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Inhibiting F-Actin Polymerization Impairs the Internalization of Moraxella catarrhalis"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3807-2088","authenticated-orcid":false,"given":"Jinhan","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China"},{"name":"Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China"},{"name":"Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China"},{"name":"Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China"},{"name":"Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9730-0744","authenticated-orcid":false,"given":"Rui","family":"Ding","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchun","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China"},{"name":"Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China"},{"name":"Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.2217\/fmb.12.80","article-title":"Moraxella catarrhalis: From interactions with the host immune system to vaccine development","volume":"7","author":"Su","year":"2012","journal-title":"Future Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1164\/rccm.200412-1747OC","article-title":"Moraxella catarrhalis in chronic obstructive pulmonary disease: Burden of disease and immune response","volume":"172","author":"Murphy","year":"2005","journal-title":"Am. 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