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Guangxi Bama miniature pig (GBM) offers an apt model for obesity-related studies. In this research, we used transcriptomics and 16S rRNA gene sequencing to discern the differentially expressed genes (DEGs) within intestinal (jejunum, ileum, and colon) tissues and variations in microbial communities in intestinal contents of GBM subjected to normal diets (ND) and high-fat, high-carbohydrate diets (HFHCD). After a feeding duration of 26 weeks, the HFHCD-fed experimental group demonstrated notable increases in backfat thickness, BMI, abnormal blood glucose metabolism, and blood lipid levels alongside the escalated serum expression of pro-inflammatory factors and a marked decline in intestinal health status when compared to the ND group. Transcriptomic analysis revealed a total of 1669 DEGs, of which 27 had similar differences in three intestinal segments across different groups, including five immune related genes: COL6A6, CYP1A1, EIF2AK2, NMI, and LGALS3B. Further, we found significant changes in the microbiota composition, with a significant decrease in beneficial bacterial populations within the HFHCD group. Finally, the results of integrated analysis of microbial diversity with transcriptomics show a positive link between certain microbial abundance (Solibacillus, norank_f__Saccharimonadaceae, Candidatus_Saccharimonas, and unclassified_f__Butyricicoccaceae) and changes in gene expression (COL6A6 and NMI). Overall, HFHCD appears to co-contribute to the initiation and progression of obesity in GBM by aggravating inflammatory responses, disrupting immune homeostasis, and creating imbalances in intestinal flora.<\/jats:p>","DOI":"10.3390\/microorganisms12020369","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T04:47:45Z","timestamp":1707713265000},"page":"369","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Integrated Analysis of the Transcriptome and Microbial Diversity in the Intestine of Miniature Pig Obesity Model"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2548-6353","authenticated-orcid":false,"given":"Wenjing","family":"Qi","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7367-9091","authenticated-orcid":false,"given":"Siran","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9818-3217","authenticated-orcid":false,"given":"Lingli","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinning","family":"Liang","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center, Guangxi Medical University, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4076-6233","authenticated-orcid":false,"given":"Xiaoping","family":"Guo","sequence":"additional","affiliation":[{"name":"Laboratory Animal Center, Guangxi Medical University, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5949-3298","authenticated-orcid":false,"given":"Feng","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafen","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganqiu","family":"Lan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5533-2107","authenticated-orcid":false,"given":"Jing","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1038\/s41574-019-0176-8","article-title":"Obesity: Global Epidemiology and Pathogenesis","volume":"15","year":"2019","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"827","DOI":"10.2174\/0929867327666200721160313","article-title":"Relationship between Gut Microbiota, Gut Hyperpermeability and Obesity","volume":"28","author":"Gasmi","year":"2021","journal-title":"Curr. Med. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e53","DOI":"10.1161\/RES.0000000000000169","article-title":"Recommendation on Design, Execution, and Reporting of Animal Atherosclerosis Studies: A Scientific Statement from the American Heart Association","volume":"121","author":"Daugherty","year":"2017","journal-title":"Circ. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.isci.2019.07.025","article-title":"Development and Genome Sequencing of a Laboratory-Inbred Miniature Pig Facilitates Study of Human Diabetic Disease","volume":"19","author":"Zhang","year":"2019","journal-title":"iScience"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1007\/s13410-021-00981-1","article-title":"Comparative Analysis of the Transcriptome of T2DM Bama Mini-Pigs with T2DM Patients","volume":"42","author":"Yan","year":"2022","journal-title":"Int. J. Diabetes Dev. Ctries."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.gene.2018.06.103","article-title":"iTRAQ and PRM-Based Quantitative Proteomics in T2DM-Susceptible and -Tolerant Models of Bama Mini-Pig","volume":"675","author":"Yan","year":"2018","journal-title":"Gene"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.gene.2018.08.051","article-title":"Comprehensive Inbred Variation Discovery in Bama Pigs Using de Novo Assemblies","volume":"679","author":"Zhang","year":"2018","journal-title":"Gene"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Casamassimi, A., Federico, A., Rienzo, M., Esposito, S., and Ciccodicola, A. (2017). Transcriptome Profiling in Human Diseases: New Advances and Perspectives. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18081652"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zolnikova, O., Dzhakhaya, N., Bueverova, E., Sedova, A., Kurbatova, A., Kryuchkova, K., Butkova, T., Izotov, A., Kulikova, L., and Yurku, K. (2023). The Contribution of the Intestinal Microbiota to the Celiac Disease Pathogenesis along with the Effectiveness of Probiotic Therapy. Microorganisms, 11.","DOI":"10.3390\/microorganisms11122848"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1016\/S1473-3099(13)70179-8","article-title":"Related Actions of Probiotics and Antibiotics on Gut Microbiota and Weight Modification","volume":"13","author":"Angelakis","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.molmet.2016.11.003","article-title":"Genome-Wide DNA Promoter Methylation and Transcriptome Analysis in Human Adipose Tissue Unravels Novel Candidate Genes for Obesity","volume":"6","author":"Keller","year":"2017","journal-title":"Mol. Metab."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1038\/s41366-018-0187-x","article-title":"Konjaku Flour Reduces Obesity in Mice by Modulating the Composition of the Gut Microbiota","volume":"43","author":"Kang","year":"2019","journal-title":"Int. J. Obes."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1007\/s11894-009-0045-z","article-title":"The Microbiome and Obesity: Is Obesity Linked to Our Gut Flora?","volume":"11","author":"Tsai","year":"2009","journal-title":"Curr. Gastroenterol. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1007\/s00335-016-9657-4","article-title":"A GWA Study Reveals Genetic Loci for Body Conformation Traits in Chinese Laiwu Pigs and Its Implications for Human BMI","volume":"27","author":"Zhou","year":"2016","journal-title":"Mamm. Genome"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1007\/BF00280883","article-title":"Homeostasis Model Assessment: Insulin Resistance and Beta-Cell Function from Fasting Plasma Glucose and Insulin Concentrations in Man","volume":"28","author":"Matthews","year":"1985","journal-title":"Diabetologia"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Li, B., and Dewey, C.N. (2011). RSEM: Accurate Transcript Quantification from RNA-Seq Data with or without a Reference Genome. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-323"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Love, M.I., Huber, W., and Anders, S. (2014). Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0550-8"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"W316","DOI":"10.1093\/nar\/gkr483","article-title":"KOBAS 2.0: A Web Server for Annotation and Identification of Enriched Pathways and Diseases","volume":"39","author":"Xie","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An Ultra-Fast All-in-One FASTQ Preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast Length Adjustment of Short Reads to Improve Genome Assemblies","volume":"27","author":"Salzberg","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-Resolution Sample Inference from Illumina Amplicon Data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing Mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Segata, N., Izard, J., Waldron, L., Gevers, D., Miropolsky, L., Garrett, W.S., and Huttenhower, C. (2011). Metagenomic Biomarker Discovery and Explanation. Genome Biol., 12.","DOI":"10.1186\/gb-2011-12-s1-p47"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.gene.2018.06.014","article-title":"Comparative Analysis on Liver Transcriptome Profiles of Different Methods to Establish Type 2 Diabetes Mellitus Models in Guangxi Bama Mini-Pig","volume":"673","author":"Wu","year":"2018","journal-title":"Gene"},{"key":"ref_26","first-page":"30","article-title":"Obesity Development in a Miniature Yucatan Pig Model: A Multi-Compartmental Metabolomics Study on Cloned and Normal Pigs Fed Restricted or Ad Libitum High-Energy Diets","volume":"18","author":"Curtasu","year":"2019","journal-title":"J. Proteome Res."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Newell-Fugate, A.E., Taibl, J.N., Alloosh, M., Sturek, M., Bahr, J.M., Nowak, R.A., and Krisher, R.L. (2015). Effects of Obesity and Metabolic Syndrome on Steroidogenesis and Folliculogenesis in the Female Ossabaw Mini-Pig. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0128749"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1002\/cbin.11015","article-title":"Rapamycin Improves Insulin Resistance and Hepatic Steatosis in Type 2 Diabetes Rats through Activation of Autophagy","volume":"42","author":"Zhou","year":"2018","journal-title":"Cell Biol. Int."},{"key":"ref_29","first-page":"44","article-title":"Serum Inflammatory Cytokines IL-1beta, IL-6, TNF-Alpha and VEGF Have Influence on the Development of Diabetic Retinopathy","volume":"53","author":"Sivkova","year":"2011","journal-title":"Folia Med. (Plovdiv)"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e5490743","DOI":"10.1155\/2020\/5490743","article-title":"Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs","volume":"2020","author":"Liu","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6081","DOI":"10.1021\/acsnano.3c01005","article-title":"Foodborne Carbon Dot Exposure Induces Insulin Resistance through Gut Microbiota Dysbiosis and Damaged Intestinal Mucus Layer","volume":"17","author":"Zhang","year":"2023","journal-title":"ACS Nano"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1056\/NEJMcibr2035081","article-title":"Obesity, Immunity, and Cancer","volume":"384","author":"Rathmell","year":"2021","journal-title":"New Engl. J. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1091000","DOI":"10.1016\/j.intimp.2022.109100","article-title":"Collagen VI Antibody Reduces Atherosclerosis by Activating Monocyte\/Macrophage Polarization in ApoE\u2212\/\u2212 Mice","volume":"111","author":"Liu","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"6103","DOI":"10.1038\/s41598-018-24213-5","article-title":"Serotonin Is an Endogenous Regulator of Intestinal CYP1A1 via AhR","volume":"8","author":"Manzella","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.molimm.2021.01.030","article-title":"EIF2AK2 Selectively Regulates the Gene Transcription in Immune Response and Histones Associated with Systemic Lupus Erythematosus","volume":"132","author":"Ge","year":"2021","journal-title":"Mol. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s10753-023-01893-4","article-title":"NMI Functions as Immuno-Regulatory Molecule in Sepsis by Regulating Multiple Signaling Pathways","volume":"47","author":"Zeng","year":"2023","journal-title":"Inflammation"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1158\/0008-5472.CAN-11-2165","article-title":"A Galectin-3-Dependent Pathway Upregulates Interleukin-6 in the Microenvironment of Human Neuroblastoma","volume":"72","author":"Silverman","year":"2012","journal-title":"Cancer Res."},{"key":"ref_38","unstructured":"(2023, November 23). Gut Microbiota in Human Adults with Type 2 Diabetes Differs from Non-Diabetic Adults\u2014PubMed, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/20140211\/."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1136\/gut.2003.025403","article-title":"Reduction in Diversity of the Colonic Mucosa Associated Bacterial Microflora in Patients with Active Inflammatory Bowel Disease","volume":"53","author":"Ott","year":"2004","journal-title":"Gut"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.2337\/dc09-0979","article-title":"Increase in Plasma Endotoxin Concentrations and the Expression of Toll-Like Receptors and Suppressor of Cytokine Signaling-3 in Mononuclear Cells After a High-Fat, High-Carbohydrate Meal: Implications for Insulin Resistance","volume":"32","author":"Ghanim","year":"2009","journal-title":"Diabetes Care"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1136\/gutjnl-2012-303839","article-title":"An Increase in the Akkermansia Spp. Population Induced by Metformin Treatment Improves Glucose Homeostasis in Diet-Induced Obese Mice","volume":"63","author":"Shin","year":"2014","journal-title":"Gut"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"9066","DOI":"10.1073\/pnas.1219451110","article-title":"Cross-Talk between Akkermansia Muciniphila and Intestinal Epithelium Controls Diet-Induced Obesity","volume":"110","author":"Everard","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2775","DOI":"10.2337\/db11-0227","article-title":"Responses of Gut Microbiota and Glucose and Lipid Metabolism to Prebiotics in Genetic Obese and Diet-Induced Leptin-Resistant Mice","volume":"60","author":"Everard","year":"2011","journal-title":"Diabetes"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1038\/oby.2012.6","article-title":"Fermentable Carbohydrate Alters Hypothalamic Neuronal Activity and Protects Against the Obesogenic Environment","volume":"20","author":"Anastasovska","year":"2012","journal-title":"Obesity"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"715506","DOI":"10.3389\/fphys.2021.715506","article-title":"Metabolic Influences of Gut Microbiota Dysbiosis on Inflammatory Bowel Disease","volume":"12","author":"Sultan","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Wang, J., Chen, W.-D., and Wang, Y.-D. (2020). The Relationship Between Gut Microbiota and Inflammatory Diseases: The Role of Macrophages. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01065"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1111\/j.0906-7590.2006.04601.x","article-title":"Combining Richness and Abundance into a Single Diversity Index Using Matrix Analogues of Shannon\u2019s and Simpson\u2019s Indices","volume":"29","author":"Gorelick","year":"2006","journal-title":"Ecography"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nature07540","article-title":"A Core Gut Microbiome in Obese and Lean Twins","volume":"457","author":"Turnbaugh","year":"2009","journal-title":"Nature"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1053\/j.gastro.2021.06.056","article-title":"Alterations in the Gut Virome in Obesity and Type 2 Diabetes Mellitus","volume":"161","author":"Yang","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Kasai, C., Sugimoto, K., Moritani, I., Tanaka, J., Oya, Y., Inoue, H., Tameda, M., Shiraki, K., Ito, M., and Takei, Y. (2015). Comparison of the Gut Microbiota Composition between Obese and Non-Obese Individuals in a Japanese Population, as Analyzed by Terminal Restriction Fragment Length Polymorphism and next-Generation Sequencing. BMC Gastroenterol., 15.","DOI":"10.1186\/s12876-015-0330-2"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1089\/omi.2019.0063","article-title":"New Insights on Obesity and Diabetes from Gut Microbiome Alterations in Egyptian Adults","volume":"23","author":"Salah","year":"2019","journal-title":"OMICS A J. Integr. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1038\/s41564-022-01224-7","article-title":"Inflammation-Associated Nitrate Facilitates Ectopic Colonization of Oral Bacterium Veillonella Parvula in the Intestine","volume":"7","author":"Brown","year":"2022","journal-title":"Nat. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2574","DOI":"10.1111\/j.1462-2920.2009.01982.x","article-title":"Towards the Human Intestinal Microbiota Phylogenetic Core","volume":"11","author":"Tap","year":"2009","journal-title":"Environ. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.chom.2014.02.005","article-title":"The Treatment-Naive Microbiome in New-Onset Crohn\u2019s Disease","volume":"15","author":"Gevers","year":"2014","journal-title":"Cell Host Microbe"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Fan, Y., Zhao, Q., Wei, Y., Wang, H., Ga, Y., Zhang, Y., and Hao, Z. (2023). Pingwei San Ameliorates Spleen Deficiency-Induced Diarrhea through Intestinal Barrier Protection and Gut Microbiota Modulation. Antioxidants, 12.","DOI":"10.3390\/antiox12051122"},{"key":"ref_56","first-page":"1156","article-title":"[High-throughput sequencing for analysis of structural change of intestinal microbiota in patients with colorectal adenoma]","volume":"37","author":"Lu","year":"2017","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"243","DOI":"10.3920\/BM2016.0090","article-title":"Lactobacillus Rhamnosus NCDC17 Ameliorates Type-2 Diabetes by Improving Gut Function, Oxidative Stress and Inflammation in High-Fat-Diet Fed and Streptozotocintreated Rats","volume":"8","author":"Singh","year":"2017","journal-title":"Benef. Microbes"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1017\/S0007114510002874","article-title":"Effects of Lactobacillus Acidophilus NCFM on Insulin Sensitivity and the Systemic Inflammatory Response in Human Subjects","volume":"104","author":"Andreasen","year":"2010","journal-title":"Br. J. Nutr."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5439","DOI":"10.1111\/1750-3841.15971","article-title":"Lactobacillus Alleviated Obesity Induced by High-Fat Diet in Mice","volume":"86","author":"Song","year":"2021","journal-title":"J. Food Sci."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Miranda-Yuquilema, J., Taboada, J., Once, V., Coyago, M., and Bri\u00f1ez, W. (2024). Effect of Agroindustrial Waste Substrate Fermented with Lactic Acid Bacteria and Yeast on Changes in the Gut Microbiota of Guinea Pigs. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010133"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"946906","DOI":"10.3389\/fvets.2022.946906","article-title":"Characterization of Gut Microbiota Dysbiosis of Diarrheic Adult Yaks through 16S rRNA Gene Sequences","volume":"9","author":"Wu","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"129981","DOI":"10.1016\/j.foodchem.2021.129981","article-title":"Egg White Peptides Ameliorate Dextran Sulfate Sodium-Induced Acute Colitis Symptoms by Inhibiting the Production of pro-Inflammatory Cytokines and Modulation of Gut Microbiota Composition","volume":"360","author":"Ge","year":"2021","journal-title":"Food Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.5650\/jos.ess22009","article-title":"The Effects of Increasing Dietary Fat on Serum Lipid Profile and Modification of Gut Microbiome in C57BL\/6N Mice","volume":"71","author":"Koontanatechanon","year":"2022","journal-title":"J. Oleo Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"11941","DOI":"10.1021\/acs.jafc.3c02696","article-title":"Anthocyanin-Rich Butterfly Pea Flower Extract Ameliorating Low-Grade Inflammation in a High-Fat-Diet and Lipopolysaccharide-Induced Mouse Model","volume":"71","author":"Yu","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.braindev.2020.09.013","article-title":"The Effect of Different Dietary Structure on Gastrointestinal Dysfunction in Children with Cerebral Palsy and Epilepsy Based on Gut Microbiota","volume":"43","author":"Huang","year":"2021","journal-title":"Brain Dev."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1097\/00004836-200603000-00015","article-title":"Colonic Health: Fermentation and Short Chain Fatty Acids","volume":"40","author":"Wong","year":"2006","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"k2179","DOI":"10.1136\/bmj.k2179","article-title":"Role of the Gut Microbiota in Nutrition and Health","volume":"361","author":"Valdes","year":"2018","journal-title":"BMJ"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1038\/nature05414","article-title":"An Obesity-Associated Gut Microbiome with Increased Capacity for Energy Harvest","volume":"444","author":"Turnbaugh","year":"2006","journal-title":"Nature"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/oby.2009.167","article-title":"Microbiota and SCFA in Lean and Overweight Healthy Subjects","volume":"18","author":"Schwiertz","year":"2010","journal-title":"Obesity (Silver Spring)"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1017\/S0007114512002723","article-title":"Higher Level of Faecal SCFA in Women Correlates with Metabolic Syndrome Risk Factors","volume":"109","author":"Teixeira","year":"2013","journal-title":"Br. J. Nutr."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1016\/j.cell.2016.05.018","article-title":"The Colonic Crypt Protects Stem Cells from Microbiota-Derived Metabolites","volume":"165","author":"Kaiko","year":"2016","journal-title":"Cell"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"155984","DOI":"10.1016\/j.scitotenv.2022.155984","article-title":"Chronic Exposure to Polyvinyl Chloride Microplastics Induces Liver Injury and Gut Microbiota Dysbiosis Based on the Integration of Liver Transcriptome Profiles and Full-Length 16S rRNA Sequencing Data","volume":"839","author":"Chen","year":"2022","journal-title":"Sci. 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