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strains of Enterococcus spp. from ovine and caprine milk. The aim of this work was to evaluate the interaction of Ma with the same Enterococcus spp. isolated from other anatomical locations (vagina) and other bacterial populations present in milk, such as coagulase-negative staphylococci (CNS). The vaginal Enterococcus strains and the raw milk CNS were isolated from sheep and goats. Experimental in vitro conditions were prepared to assess the growth of Ma with and without the presence of these strains. The selected vaginal strains were identified as Enterococcus (E.) hirae and E. mundtii, and the strains of CNS were identified as Staphylococcus petrasii. Different interactions of Ma with ovine and caprine wild vaginal strains of Enterococcus and dairy strains of CNS are described for the first time: Ma can grow exponentially during 15 h with the selected strains, although with certain strains, its optimal growth can be negatively affected (p &lt; 0.05). The colonization and\/or excretion of Ma could, therefore, be influenced by certain endogenous bacterial strains. Our results increase the knowledge about possible bacterial ecology dynamics surrounding CA.<\/jats:p>","DOI":"10.3390\/microorganisms12020406","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T11:23:57Z","timestamp":1708341837000},"page":"406","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In Vitro Interaction between Mycoplasma agalactiae and Small Ruminants\u2019 Endogenous Bacterial Strains of Enterococcus spp. and Coagulase-Negative Staphylococcus"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3243-3711","authenticated-orcid":false,"given":"Marion","family":"Toquet","sequence":"first","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producci\u00f3n y Sanidad Animal, Salud P\u00fablica Veterinaria y Ciencia y Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Bataller","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producci\u00f3n y Sanidad Animal, Salud P\u00fablica Veterinaria y Ciencia y Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4268-7846","authenticated-orcid":false,"given":"Raquel","family":"Toledo-Perona","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producci\u00f3n y Sanidad Animal, Salud P\u00fablica Veterinaria y Ciencia y Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8349-1607","authenticated-orcid":false,"given":"Jes\u00fas","family":"Gomis","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producci\u00f3n y Sanidad Animal, Salud P\u00fablica Veterinaria y Ciencia y Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Contreras","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1868-3829","authenticated-orcid":false,"given":"Antonio","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0320-4813","authenticated-orcid":false,"given":"Estrella","family":"Jim\u00e9nez-Trigos","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producci\u00f3n y Sanidad Animal, Salud P\u00fablica Veterinaria y Ciencia y Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4483-3077","authenticated-orcid":false,"given":"\u00c1ngel","family":"G\u00f3mez-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Microbiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producci\u00f3n y Sanidad Animal, Salud P\u00fablica Veterinaria y Ciencia y Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"848","DOI":"10.20506\/rst.16.3.1062","article-title":"Contagious Agalactia of Small Ruminants: Current Knowledge Concerning Epidemiology, Diagnosis and Control","volume":"16","author":"Bergonier","year":"1997","journal-title":"Rev. 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