{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:14:19Z","timestamp":1789708459609,"version":"4.0.1"},"reference-count":68,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EnvEast DTP","award":["NE\/L002582\/1"],"award-info":[{"award-number":["NE\/L002582\/1"]}]},{"name":"EnvEast DTP","award":["DE-SC0024289"],"award-info":[{"award-number":["DE-SC0024289"]}]},{"name":"Norfolk County Council","award":["NE\/L002582\/1"],"award-info":[{"award-number":["NE\/L002582\/1"]}]},{"name":"Norfolk County Council","award":["DE-SC0024289"],"award-info":[{"award-number":["DE-SC0024289"]}]},{"name":"U.S. Department of Energy under the DOE Office of Science (SC) RENEW contract","award":["NE\/L002582\/1"],"award-info":[{"award-number":["NE\/L002582\/1"]}]},{"name":"U.S. Department of Energy under the DOE Office of Science (SC) RENEW contract","award":["DE-SC0024289"],"award-info":[{"award-number":["DE-SC0024289"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Considering the increasing interest in understanding the biotic component of methane removal from our atmosphere, it becomes essential to study the physiological characteristics and genomic potential of methanotroph isolates, especially their traits allowing them to adapt to elevated growth temperatures. The genetic signatures of Methylocaldum species have been detected in many terrestrial and aquatic ecosystems. A small set of representatives of this genus has been isolated and maintained in culture. The genus is commonly described as moderately thermophilic, with the growth optimum reaching 50 \u00b0C for some strains. Here, we present a comparative analysis of genomes of three Methylocaldum strains\u2014two terrestrial M. szegediense strains (O-12 and Norfolk) and one marine strain, Methylocaldum marinum (S8). The examination of the core genome inventory of this genus uncovers significant redundancy in primary metabolic pathways, including the machinery for methane oxidation (numerous copies of pmo genes) and methanol oxidation (duplications of mxaF, xoxF1-5 genes), three pathways for one-carbon (C1) assimilation, and two methods of carbon storage (glycogen and polyhydroxyalkanoates). We also investigate the genetics of melanin production pathways as a key feature of the genus.<\/jats:p>","DOI":"10.3390\/microorganisms12030469","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T08:59:01Z","timestamp":1708937941000},"page":"469","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Genomic Insights into Moderately Thermophilic Methanotrophs of the Genus Methylocaldum"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3906-7301","authenticated-orcid":false,"given":"Nathalie A.","family":"Delherbe","sequence":"first","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA 92129, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Pearce","sequence":"additional","affiliation":[{"name":"School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2788-8842","authenticated-orcid":false,"given":"Sergey Y.","family":"But","sequence":"additional","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Scientific Center for Biological Research of the Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Colin","family":"Murrell","sequence":"additional","affiliation":[{"name":"School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina N.","family":"Khmelenina","sequence":"additional","affiliation":[{"name":"G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Scientific Center for Biological Research of the Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9058-7794","authenticated-orcid":false,"given":"Marina G.","family":"Kalyuzhnaya","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University, San Diego, CA 92129, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/nature06411","article-title":"Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia","volume":"450","author":"Dunfield","year":"2007","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1073\/pnas.0704162105","article-title":"Methane oxidation at 55 C and pH 2 by a thermoacidophilic bacterium belonging to the Verrucomicrobia phylum","volume":"105","author":"Islam","year":"2008","journal-title":"Proc. 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