{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:56:43Z","timestamp":1787029003612,"version":"build-2736575974"},"reference-count":23,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"RKI budget"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The effect of oxidative stress on the survival of various Brucella species has not been fully investigated yet. We here conducted a study in which we investigated the effect of different types of oxidative stress (Fe2+, H2O2, bleach) versus non-oxidative inhibitory effects (selenite, erythritol, and isopropanol) on the survival of B. abortus S19, B. abortus S19 \u2206mglA 3.14, and B. neotomae 5K33. The work focuses on the appearance of ATP\u2013CFU quotient imbalances indicating the existence of a viable but nonculturable (VBNC) form of B. abortus S19, as has previously been shown.<\/jats:p>","DOI":"10.3390\/microorganisms12030491","type":"journal-article","created":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T02:59:39Z","timestamp":1709175579000},"page":"491","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evidence of a Viable but Nonculturable (VBNC) Phase in B. abortus S19 under Oxidative Stress (H2O2, -Fe2+, Bleach) and under Non-Oxidative Inhibitory Conditions (Isopropanol, Erythritol, Selenite)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7039-0735","authenticated-orcid":false,"given":"Jens","family":"Jacob","sequence":"first","affiliation":[{"name":"Unit 14 Hospital Hygiene and Infection Prevention and Control, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1007\/s12223-020-00798-1","article-title":"Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages","volume":"65","author":"Jacob","year":"2020","journal-title":"Folia Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1007\/0-387-30745-1_17","article-title":"The genus Brucella","volume":"5","author":"Moreno","year":"2006","journal-title":"Prokaryotes"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1016\/S0264-410X(98)00063-2","article-title":"The defect in the metabolism of erythritol of the Brucella abortus B19 vaccine strain is unrelated with its attenuated virulence in mice","volume":"16","author":"Sangari","year":"1998","journal-title":"Vaccine"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1080\/1040841X.2017.1332002","article-title":"Brucella central carbon metabolism: An update","volume":"44","author":"Barbier","year":"2018","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1128\/aem.52.3.531-538.1986","article-title":"Viable but nonculturable stage of Campylobacter jejuni and its role in survival in the natural aquatic environment","volume":"52","author":"Rollins","year":"1986","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0232-4393(11)80073-9","article-title":"Characterization of Viable but Nonculturable Stage of C. coli, characterized with respect to Electron Microscopic findings, Whole Cell Protein and Lipooligosaccharide patterns","volume":"148","author":"Jacob","year":"1993","journal-title":"Zentralbl. Mikrobiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e12391","DOI":"10.1111\/hel.12391","article-title":"Reduced infectivity of waterborne viable but nonculturable Helicobacter pylori strain SS1 in mice","volume":"22","author":"Boehnke","year":"2017","journal-title":"Helicobacter"},{"key":"ref_8","first-page":"103","article-title":"Is the coccoid form of Helicobacter pylori viable?","volume":"87","author":"Hua","year":"1996","journal-title":"Microbios"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/j.1574-6941.2000.tb00686.x","article-title":"Francisella tularensis does not manifest virulence in viable but non-culturable state","volume":"31","author":"Forsman","year":"2000","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_10","unstructured":"Linke, S. (2012). Untersuchungen zur Erh\u00f6hten Toleranz von Biofilmassoziierten Mikroorganismen und Die Ausbildung von VBNC-Zust\u00e4nden bei Pseudomonas aeruginosa Gegen\u00fcber Chlorabspaltenden Verbindungen. [Ph.D. Thesis, Universit\u00e4t Bonn]."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3754","DOI":"10.1128\/AEM.65.8.3754-3756.1999","article-title":"The Viable-but-Nonculturable Condition is induced by Copper in Agrobacterium tumefaciens and Rhizobium leguminosarum","volume":"65","author":"Alexander","year":"1999","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1007\/s003740000195","article-title":"Energy content decrease and viable-not-culturable status induced by oxygen limitation coupled to the presence of nitrogen oxides in Rhizobium \u201chedysari\u201d","volume":"31","author":"Toffanin","year":"2000","journal-title":"Biol. Fertil. Soils"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Crasta, O.R., Folkerts, O., Fei, Z., Mane, S.P., Evans, C., Martino-Catt, S., Bricker, B., Yu, G., Du, L., and Sobral, B.W. (2008). Genome sequence of Brucella abortus vaccine candidate S19 compared to virulent strains yields candidate virulence genes. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0002193"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.ijmm.2012.02.001","article-title":"The mglA gene and its flanking regions in Brucella: The role of mglA in tolerance to hostile environments, Fe-metabolism and in vivo persistence","volume":"302","author":"Jacob","year":"2012","journal-title":"Int. J. Med Microbiol."},{"key":"ref_15","first-page":"47","article-title":"A new species of Brucella isolated from the desert wood rat, Neotoma lepida Thomas","volume":"99","author":"Stoenner","year":"1957","journal-title":"Am. J.Vet. Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Audic, S., Lescot, M., Claverie, J.-M., and Scholz, H.C. (2009). Brucella microti: The genome sequence of an emerging pathogen. BMC Genom., 10.","DOI":"10.1186\/1471-2164-10-352"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5837","DOI":"10.1128\/AEM.00620-10","article-title":"Characterization of novel Brucella strains originating from wild native rodent species in North Queensland, Australia","volume":"76","author":"Tiller","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_18","first-page":"703813","article-title":"Viable but Nonculturable Bacteria: Food Safety and Public Health Perspective","volume":"2013","author":"Fakruddin","year":"2013","journal-title":"Int. Sch. Res. Not."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/j.1574-6976.2009.00200.x","article-title":"Recent findings on the viable but nonculturable state in pathogenic bacteria","volume":"34","author":"Oliver","year":"2009","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3389\/fpubh.2014.00103","article-title":"Current perspectives on viable but non-culturable (VBNC) pathogenic bacteria","volume":"2","author":"Ramamurthy","year":"2014","journal-title":"Front. Public Health"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"124335","DOI":"10.1016\/j.jhazmat.2020.124335","article-title":"Detection and distribution of VBNC\/viable pathogenic bacteria in full-scale drinking water treatment plants","volume":"406","author":"Guo","year":"2021","journal-title":"J. Hazard. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jhazmat.2021.127228","article-title":"Mechanisms underlying the effect of chlorination and UV disinfection on VBNC state Escherichia coli isolated from hospital wastewater","volume":"423","author":"Zhu","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11157-020-09522-4","article-title":"Challenges of unculturable bacteria: Environmental perspectives","volume":"19","author":"Bodor","year":"2020","journal-title":"Rev. Environ. Sci. Bio\/Technol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/3\/491\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:06:45Z","timestamp":1760105205000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/3\/491"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,28]]},"references-count":23,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["microorganisms12030491"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12030491","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,28]]}}}