{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T05:48:02Z","timestamp":1771998482290,"version":"3.50.1"},"reference-count":22,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T00:00:00Z","timestamp":1710115200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>In this study, we have compared the detection of IgM and IgG against C. burnetii phase II of an enzyme-linked immunosorbent assay (ELISA) (Euroimmun) and a chemiluminescent immunoassay (CLIA) (VIRCLIA, Vircell). In addition, an indirect immunofluorescence assay (IFA) was used as a reference test. One hundred forty-eight sera were used for IgG evaluation, and eighty-eight for IgM. The sensitivity of ELISA and CLIA in detecting phase II IgM was excellent. On the other hand, the CLIA IgM showed better specificity than the ELISA IgM. As for phase II IgG, the specificity of ELISA and CLIA was similar, while the ELISA technique showed a higher sensitivity. In conclusion, the best system to detect phase II IgM antibodies against C. burnetii is the CLIA from Vircell, which is characterized by high sensitivity and specificity. For the detection of phase II IgG, the Euroimmun ELISA and Vircell CLIA assays are suitable for the determination of this marker in the laboratory, although the IgG ELISA has greater sensitivity.<\/jats:p>","DOI":"10.3390\/microorganisms12030552","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T07:25:32Z","timestamp":1710141932000},"page":"552","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparison of an Enzyme Linked-Immunosorbent Assay and a Chemiluminescent Immunoassay with an Immunofluorescence Assay for Detection of Phase II IgM and IgG Antibodies to Coxiella burnetii"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1374-0756","authenticated-orcid":false,"given":"Juan Francisco","family":"Guti\u00e9rrez-Bautista","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular e Inmunolog\u00eda III, University of Granada, 18016 Granada, Spain"},{"name":"Servicio de An\u00e1lisis Cl\u00ednicos e Inmunolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Tarri\u00f1o","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adri\u00e1n","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Olivares Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Servicio de An\u00e1lisis Cl\u00ednicos e Inmunolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9203-9628","authenticated-orcid":false,"given":"Fernando","family":"Cobo","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Servicio de Microbiolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Antonio","family":"Reguera","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Servicio de Microbiolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Rodr\u00edguez-Granger","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Servicio de Microbiolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Sampedro","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Servicio de Microbiolog\u00eda, University Hospital Virgen de las Nieves, 18014 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1068129","DOI":"10.3389\/fvets.2022.1068129","article-title":"Coxiella burnetii in Ticks, Livestock, Pets and Wildlife: A Mini-Review","volume":"9","author":"Celina","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00045-16","article-title":"From Q Fever to Coxiella burnetii Infection: A Paradigm Change","volume":"30","author":"Eldin","year":"2017","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1086\/321878","article-title":"Sexually Transmitted Q Fever","volume":"33","author":"Milazzo","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","unstructured":"(2024, March 08). Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/q-fever-annual-epidemiological-report-2019."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1183\/09031936.93.02030263","article-title":"Q Fever Pneumonia: A Review of 164 Community-Acquired Cases in the Basque Country","volume":"2","author":"Sobradillo","year":"1989","journal-title":"Eur. Respir. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1128\/JCM.36.7.1823-1834.1998","article-title":"Diagnosis of Q Fever","volume":"36","author":"Fournier","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"S253","DOI":"10.1093\/clind\/21.Supplement_3.S253","article-title":"Coxiella burnetii (Q Fever) Pneumonia","volume":"21","author":"Marrie","year":"1995","journal-title":"Clin. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1093\/cid\/cit419","article-title":"Evolution from Acute Q Fever to Endocarditis Is Associated with Underlying Valvulopathy and Age and Can Be Prevented by Prolonged Antibiotic Treatment","volume":"57","author":"Million","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1128\/iai.43.1.421-428.1984","article-title":"Monoclonal Antibodies Distinguish Phase Variants of Coxiella burnetii","volume":"43","author":"Williams","year":"1984","journal-title":"Infect. Immun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.5694\/j.1326-5377.1970.tb84481.x","article-title":"The persistence of complement-fixing antibodies to q\u2013fever (Coxiella burneti) after infection","volume":"1","author":"Murphy","year":"1970","journal-title":"Med. J. Aust."},{"key":"ref_11","first-page":"1","article-title":"Diagnosis and Management of Q Fever\u2014United States, 2013: Recommendations from CDC and the Q Fever Working Group","volume":"62","author":"Anderson","year":"2013","journal-title":"Morb. Mortal. Wkly. Rep. Recomm. Rep."},{"key":"ref_12","first-page":"A2388","article-title":"Laboratory Diagnosis of Acute Q Fever","volume":"154","author":"Horrevorts","year":"2010","journal-title":"Ned. Tijdschr. Geneeskd."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1128\/CVI.05581-11","article-title":"Evaluation of Commonly Used Serological Tests for Detection of Coxiella burnetii Antibodies in Well-Defined Acute and Follow-up Sera","volume":"19","author":"Wielders","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"La Scola, B., and Raoult, D. (1996). Serological Cross-Reactions between Bartonella quintana, Bartonella henselae, and Coxiella burnetii. J. Clin. Microbiol., 34.","DOI":"10.1128\/jcm.34.9.2270-2274.1996"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/cdli.4.2.208-212.1997","article-title":"Serological Cross-Reactions between Coxiella burnetii and Legionella micdadei","volume":"4","author":"Musso","year":"1997","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1007\/BF02148690","article-title":"Evaluation of the Complement Fixation and Indirect Immunofluorescence Tests in the Early Diagnosis of Primary Q Fever","volume":"4","author":"Peter","year":"1985","journal-title":"Eur. J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.2307\/2529310","article-title":"The Measurement of Observer Agreement for Categorical Data","volume":"33","author":"Landis","year":"1977","journal-title":"Biometrics"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1093\/cid\/cir132","article-title":"Chronic Q Fever: Different Serological Results in 3 Countries\u2014Results of a Follow-up Study 6 Years after a Point Source Outbreak","volume":"52","author":"Healy","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/JCM.00951-19","article-title":"Comparison of Different Commercially Available Enzyme-Linked Immunosorbent Assays with Immunofluorescence Test for Detection of Phase II IgG and IgM Antibodies to Coxiella burnetii","volume":"58","author":"Dangel","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1128\/JCM.38.4.1645-1647.2000","article-title":"Comparison of a Commercial Enzyme-Linked Immunosorbent Assay with Immunofluorescence and Complement Fixation Tests for Detection of Coxiella burnetii (Q Fever) Immunoglobulin M","volume":"38","author":"Field","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1128\/jcm.26.10.1978-1982.1988","article-title":"Enzyme-Linked Immunosorbent Assay for Diagnosis of Chronic Q Fever","volume":"26","author":"Peter","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1128\/cdli.4.3.384-386.1997","article-title":"Combined Determination of Coxiella burnetii-Specific Immunoglobulin M (IgM) and IgA Improves Specificity in the Diagnosis of Acute Q Fever","volume":"4","author":"Devine","year":"1997","journal-title":"Clin. Diagn. Lab. 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