{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T12:14:13Z","timestamp":1786968853511,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["CX(23)1006"],"award-info":[{"award-number":["CX(23)1006"]}]},{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["JATS [2023] 458"],"award-info":[{"award-number":["JATS [2023] 458"]}]},{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["JATS [2023] 461"],"award-info":[{"award-number":["JATS [2023] 461"]}]},{"name":"Jiangsu Agriculture Science and Technology Innovation Fund","award":["JBGS [2021] 120"],"award-info":[{"award-number":["JBGS [2021] 120"]}]},{"name":"earmarked fund for Jiangsu Agricultural Industry Technology System","award":["CX(23)1006"],"award-info":[{"award-number":["CX(23)1006"]}]},{"name":"earmarked fund for Jiangsu Agricultural Industry Technology System","award":["JATS [2023] 458"],"award-info":[{"award-number":["JATS [2023] 458"]}]},{"name":"earmarked fund for Jiangsu Agricultural Industry Technology System","award":["JATS [2023] 461"],"award-info":[{"award-number":["JATS [2023] 461"]}]},{"name":"earmarked fund for Jiangsu Agricultural Industry Technology System","award":["JBGS [2021] 120"],"award-info":[{"award-number":["JBGS [2021] 120"]}]},{"name":"\u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["CX(23)1006"],"award-info":[{"award-number":["CX(23)1006"]}]},{"name":"\u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["JATS [2023] 458"],"award-info":[{"award-number":["JATS [2023] 458"]}]},{"name":"\u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["JATS [2023] 461"],"award-info":[{"award-number":["JATS [2023] 461"]}]},{"name":"\u201cJBGS\u201d Project of Seed Industry Revitalization in Jiangsu Province","award":["JBGS [2021] 120"],"award-info":[{"award-number":["JBGS [2021] 120"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Aeromonas veronii is widespread in aquatic environments and is responsible for infecting various aquatic animals. In this study, a dominant strain was isolated from the hepatopancreas of diseased Macrobrachium rosenbergii and was named JDM1-1. According to its morphological, physiological, and biochemical characteristics and molecular identification, isolate JDM1-1 was identified as A. veronii. The results of artificial challenge showed isolate JDM1-1 had high pathogenicity to M. rosenbergii with an LD50 value of 8.35 \u00d7 105 CFU\/mL during the challenge test. Histopathological analysis revealed severe damage in the hepatopancreas and gills of the diseased prawns, characterized by the enlargement of the hepatic tubule lumen and gaps between the tubules as well as clubbing and degeneration observed at the distal end of the gill filament. Eight virulence-related genes, namely aer, ompA, lip, tapA, hlyA, flgA, flgM, and flgN, were screened by PCR assay. In addition, virulence factor detection showed that the JDM1-1 isolate produced lipase, lecithinase, gelatinase, and hemolysin. Furthermore, the mRNA expression profiles of immune-related genes of M. rosenbergii following A. veronii infection, including ALF1, ALF2, Crustin, C-lectin, and Lysozyme, were assessed, and the results revealed a significant upregulation in the hepatopancreas and intestines at different hours post infection. This study demonstrates that A. veronii is a causative agent associated with massive die-offs of M. rosenbergii and contributes valuable insights into the pathogenesis and host defense mechanisms of A. veronii invasion.<\/jats:p>","DOI":"10.3390\/microorganisms12040694","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T06:33:16Z","timestamp":1711693996000},"page":"694","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Pathogenicity of Aeromonas veronii Isolated from Diseased Macrobrachium rosenbergii and Host Immune-Related Gene Expression Profiles"],"prefix":"10.3390","volume":"12","author":[{"given":"Xiaojian","family":"Gao","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zirui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qieqi","family":"Qian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anting","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijie","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinzhe","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,29]]},"reference":[{"key":"ref_1","unstructured":"Fisheries Bureau, Department of Agriculture of China (2023). 2022 China Fishery Statistical Yearbook, China Agriculture Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1111\/raq.12754","article-title":"Diseases of the giant river prawn Macrobrachium rosenbergii: A review for a growing industry","volume":"15","author":"Hooper","year":"2023","journal-title":"Rev. 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