{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:20:38Z","timestamp":1791836438356,"version":"4.3.4"},"reference-count":36,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:00:00Z","timestamp":1713830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["421385\/2023-5"],"award-info":[{"award-number":["421385\/2023-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","award":["08\/2018"],"award-info":[{"award-number":["08\/2018"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FAPEPI Ed.","award":["421385\/2023-5"],"award-info":[{"award-number":["421385\/2023-5"]}]},{"name":"FAPEPI Ed.","award":["08\/2018"],"award-info":[{"award-number":["08\/2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Postbiotic is the term used to define the soluble factors, metabolic products, or byproducts released by live probiotic bacteria or after its lysis. The objective of this study was to carry out the chemical characterization of the postbiotic of Lacticaseibacillus rhamnosus LR-32 and to evaluate its in vitro effect on the development of the Streptococcus mutans biofilm. After the cultivation of the probiotic strain, the postbiotic was extracted by centrifuging the culture and filtering the supernatant. This postbiotic was characterized by using gas chromatography coupled with mass spectrometry (GC\u2013MS), and then it was used to determine the growth inhibition of S. mutans in its planktonic form; additionally, its effects on the following parameters in 48 h biofilm were evaluated: viable bacteria, dry weight, and gene expression of glucosyltransferases and VicR gene. The control group consisted of the biofilm without any treatment. A paired t-test was performed for statistical analysis, with the p-value set at 5%. Seventeen compounds of various chemical classes were identified in the postbiotic, including sugars, amino acids, vitamins, and acids. The treatment with the postbiotic led to an inhibition of the growth of S. mutans in its planktonic form, as well as a decrease in the number of viable bacteria, reduction in dry weight, and a negative regulation of the gene expression of gtfB, gtfC, gtfD, and vicR in its biofilm state, compared with the nontreated group (p &lt; 0.05). The postbiotic of L. rhamnosus impaired the development of S. mutans biofilm.<\/jats:p>","DOI":"10.3390\/microorganisms12050843","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T08:08:27Z","timestamp":1713859707000},"page":"843","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Chemical Characterization and Effect of a Lactobacilli-Postbiotic on Streptococcus mutans Biofilm In Vitro"],"prefix":"10.3390","volume":"12","author":[{"given":"Guilherme Bandeira","family":"Santana","sequence":"first","affiliation":[{"name":"Postgraduate Program in Dentistry, Federal University of Piau\u00ed, Teresina 64049-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9729-6909","authenticated-orcid":false,"given":"Patrick Veras","family":"Quelemes","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Dentistry, Federal University of Piau\u00ed, Teresina 64049-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enedina Rodrigues","family":"da Silva Neta","sequence":"additional","affiliation":[{"name":"Organic Geochemistry Laboratory, Postgraduate Program in Chemistry, Federal University of Piau\u00ed, Teresina 64049-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8754-1499","authenticated-orcid":false,"given":"Sidney Gon\u00e7alo","family":"de Lima","sequence":"additional","affiliation":[{"name":"Organic Geochemistry Laboratory, Postgraduate Program in Chemistry, Federal University of Piau\u00ed, Teresina 64049-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6055-9493","authenticated-orcid":false,"given":"Gl\u00e1uber Campos","family":"Vale","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Dentistry, Federal University of Piau\u00ed, Teresina 64049-550, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/nrgastro.2014.66","article-title":"Expert consensus document. 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