{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:27:31Z","timestamp":1790738851422,"version":"4.1.0"},"reference-count":49,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T00:00:00Z","timestamp":1714348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation program","award":["101000831"],"award-info":[{"award-number":["101000831"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Higher plants produce secondary metabolites expressing antimicrobial effects as a defense mechanism against opportunistic microorganisms living in close proximity with the plant. Fermentation leads to bioconversion of plant substrates to these bioactive compounds and their subsequent release via breakdown of plant cell walls. Fermented feed products have recently started to become implemented in the pig industry to reduce overall disease pressure and have been found to reduce events such as post-weaning diarrhea. In this study, we investigate the antimicrobial potential of fermented soybean- and rapeseed-based pig feed supplements with and without added seaweed. The antimicrobial effect was tested in a plate well diffusion assay against a range of known human and livestock pathogenic bacteria. Further, we investigate the metabolite profiles based on liquid-chromatography mass-spectrometry (LC-MS) analysis of the fermented products in comparison to their unfermented constituents. We observed a pronounced release of potential antimicrobial secondary metabolites such as benzoic acids when the plant material was fermented, and a significantly increased antimicrobial effect compared to the unfermented controls against several pathogenic bacteria, especially Salmonella enterica Typhimurium, Listeria monocytogenes, Yersinia enterocolitica, and a strain of atopic dermatitis causing Staphylococcus aureus CC1. In conclusion, fermentation significantly enhances the antimicrobial properties of rapeseed, soybean, and seaweed, offering a promising alternative to zinc oxide for controlling pathogens in piglet feed. This effect is attributed to the release of bioactive metabolites effective against pig production-relevant bacteria.<\/jats:p>","DOI":"10.3390\/microorganisms12050891","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T08:42:57Z","timestamp":1714639377000},"page":"891","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Fermented Rapeseed and Soybean Alone and in Combination with Macro Algae Inhibit Human and Pig Pathogenic Bacteria In Vitro"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4502-5688","authenticated-orcid":false,"given":"Frederik","family":"Beck","sequence":"first","affiliation":[{"name":"Fermentationexperts, Vorbassevej 12, 6622 Baekke, Denmark"},{"name":"Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1959 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0861-2728","authenticated-orcid":false,"given":"Ninfa Rangel","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Fermentationexperts, Vorbassevej 12, 6622 Baekke, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8121-1114","authenticated-orcid":false,"given":"Dennis Sandris","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1959 Frederiksberg C, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Campbell, J.M., Crenshaw, J.D., and Polo, J. (2013). The biological stress of early weaned piglets. J. Anim. Sci. Biotechnol., 4.","DOI":"10.1186\/2049-1891-4-19"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.yrtph.2006.02.004","article-title":"Probiotics for animal nutrition in the European Union. Regulation and safety assessment","volume":"45","year":"2006","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.envpol.2016.03.034","article-title":"National monitoring study in Denmark finds increased and critical levels of copper and zinc in arable soils fertilized with pig slurry","volume":"214","author":"Jensen","year":"2016","journal-title":"Environ. Pollut."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ciesinski, L., Guenther, S., Pieper, R., Kalisch, M., Bednorz, C., and Wieler, L.H. (2018). High dietary zinc feeding promotes persistence of multi-resistant E. coli in the swine gut. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191660"},{"key":"ref_5","unstructured":"Heinzl, I., Barbosa, F.F., and Nutrition, E.W. (2020, February 01). The Zinc Oxide Ban: What Led to It, What Are the Alternatives? Zinc Oxide Disadvantages Outweigh Advantages. Available online: https:\/\/ew-nutrition.com\/en-uk\/page\/3\/?s=how+to+get+cash+from+paypal+instantlyVisit+Site+Sig8.comhow+to+transfer+paypal+to+gcashhow+to+transfer+money+from+bank+account+to+paypal+instantlysending+money+through+paypalhow+to+pay+someone+on+paypalpaypal+flipstransfer+money+from+credit+card+to+paypalpaypal+wire+transferpay+someone+with+paypalhow+to+transfer+money+from+paypal+to+bankhow+to+receive+money+on+paypal+from+a+friend....a09f&print=print-search."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ming, D., Wang, W., Huang, C., Wang, Z., Shi, C., Ding, J., and Wang, F. (2021). Effects of Weaning Age at 21 and 28 Days on Growth Performance, Intestinal Morphology and Redox Status in Piglets. Animals, 11.","DOI":"10.3390\/ani11082169"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Satessa, G.D., Tamez-Hidalgo, P., Hui, Y., Cieplak, T., Krych, L., Kj\u00e6rulff, S., and Nielsen, M.O. (2020). Impact of Dietary Supplementation of Lactic Acid Bacteria Fermented Rapeseed with or without Macroalgae on Performance and Health of Piglets Following Omission of Medicinal Zinc from Weaner Diets. Animals, 10.","DOI":"10.3390\/ani10010137"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Satessa, G.D., Tamez-Hidalgo, P., Kj\u00e6rulff, S., Vargas-Bello-P\u00e9rez, E., Dhakal, R., and Nielsen, M.O. (2020). Effects of increasing doses of Lactobacillus pre-fermented rapeseed product with or without inclusion of macroalgae product on Weaner piglet performance and intestinal development. Animals, 10.","DOI":"10.3390\/ani10040559"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hui, Y., Tamez-Hidalgo, P., Cieplak, T., Satessa, G.D., Kot, W., Kj\u00e6rulff, S., and Krych, L. (2021). Supplementation of a lacto-fermented rapeseed-seaweed blend promotes gut microbial-and gut immune-modulation in weaner piglets. J. Anim. Sci. Biotechnol., 12.","DOI":"10.1186\/s40104-021-00601-2"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1007\/s10620-006-9705-0","article-title":"Effect of fermented soybean meal on intestinal morphology and digestive enzyme activities in weaned piglets","volume":"52","author":"Feng","year":"2007","journal-title":"Dig. Dis. Sci."},{"key":"ref_11","first-page":"3996","article-title":"Amino acid and phosphorus digestibility of fermented corn-soybean meal mixed feed with Bacillus subtilis and Enterococcus faecium fed to pigs1","volume":"95","author":"Shi","year":"2017","journal-title":"J. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.anaerobe.2009.04.002","article-title":"Fermented liquid feed enhances bacterial diversity in piglet intestine","volume":"16","author":"Tajima","year":"2010","journal-title":"Anaerobe"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kumar, V., Ahluwalia, V., Saran, S., Kumar, J., Patel, A.K., and Singhania, R.R. (2021). Recent developments on solid-state fermentation for production of microbial secondary metabolites: Challenges and solutions. Bioresour. Technol., 323.","DOI":"10.1016\/j.biortech.2020.124566"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.4315\/0362-028X-66.10.1811","article-title":"Antibacterial Activities of Phenolic Benzaldehydes and Benzoic Acids against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica","volume":"66","author":"Friedman","year":"2003","journal-title":"J. Food Prot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0924-8579(97)00051-4","article-title":"Inhibition of Listeria monocytogenes and Salmonella enteriditis by combinations of plant oils and derivatives of benzoic acid: The development of synergistic antimicrobial combinations1The work in this manuscript is the subject of a patent application.1","volume":"9","author":"Fyfe","year":"1998","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.lwt.2017.05.075","article-title":"Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enhanced antibacterial activity in beef cutlet against Salmonella typhimurium during refrigerated storage","volume":"84","author":"Hadian","year":"2017","journal-title":"LWT"},{"key":"ref_17","first-page":"204","article-title":"Isolation and characterization of benzoic acid with antimicrobial activity from needle of Pinus densiflora","volume":"29","author":"Kuk","year":"1997","journal-title":"Korean J. Food Sci. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Xie, Z., Hu, L., Li, Y., Geng, S., Cheng, S., Fu, X., and Han, X. (2017). Changes of gut microbiota structure and morphology in weaned piglets treated with fresh fermented soybean meal. World J. Microbiol. Biotechnol., 33.","DOI":"10.1007\/s11274-017-2374-7"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1097\/MCO.0b013e32835665fa","article-title":"Butyrate: Implications for intestinal function","volume":"15","author":"Leonel","year":"2012","journal-title":"Curr. Opin. Clin. Nutr. Metab. Care"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/j.1365-2036.2007.03562.x","article-title":"Review article: The role of butyrate on colonic function","volume":"27","author":"Hamer","year":"2008","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1017\/S0029665107005484","article-title":"Nutritional management of gut health in pigs around weaning","volume":"66","author":"Bosi","year":"2007","journal-title":"Proc. Nutr. Soc."},{"key":"ref_22","first-page":"204","article-title":"Competitive exclusion treatment reduces the mortality and fecal shedding associated with enterotoxigenic Escherichia coli infection in nursery-raised neonatal pigs","volume":"64","author":"Genovese","year":"2000","journal-title":"Can. J. Vet. Res. Rev. Can. Rech. Vet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.4315\/0362-028X-66.8.1353","article-title":"Competitive Exclusion of Salmonella from the Gut of Neonatal and Weaned Pigs","volume":"66","author":"Genovese","year":"2003","journal-title":"J. Food Prot."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1080\/00071660802538632","article-title":"Managing gut health through nutrition","volume":"50","author":"Choct","year":"2009","journal-title":"Br. Poult. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1136\/gut.42.6.799","article-title":"Dietary fibre and intestinal microflora: Effects on intestinal morphometry and crypt branching","volume":"42","author":"McCullough","year":"1998","journal-title":"Gut"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0899-9007(97)00385-7","article-title":"Gut permeability, intestinal morphology, and nutritional depletion","volume":"14","author":"Thunnissen","year":"1998","journal-title":"Nutrition"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3115","DOI":"10.1007\/s10811-019-01781-1","article-title":"Biosprospecting potential of kelp (Laminariales, Phaeophyceae) from Baja California Peninsula: Phenolic content, antioxidant properties, anti-inflammatory, and cell viability","volume":"31","author":"Ascencio","year":"2019","journal-title":"J. Appl. Phycol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1002\/elsc.201400191","article-title":"Algae as production systems of bioactive compounds","volume":"15","author":"Michalak","year":"2015","journal-title":"Eng. Life Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1007\/s10811-018-1712-y","article-title":"Enzyme-assisted extraction of proteins from the seaweeds Macrocystis pyrifera and Chondracanthus chamissoi: Characterization of the extracts and their bioactive potential","volume":"31","author":"Bernal","year":"2019","journal-title":"J. Appl. Phycol."},{"key":"ref_30","first-page":"232","article-title":"Role of secondary metabolites in defense mechanisms of plants","volume":"3","author":"Mazid","year":"2011","journal-title":"Biol. Med."},{"key":"ref_31","first-page":"923","article-title":"Bioactive Compounds Produced by Dunaliella species, Antimicrobial Effects and Optimization of the Efficiency","volume":"19","author":"Kilic","year":"2018","journal-title":"Turk. J. Fish. Aquat. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Lelario, F., Scrano, L., De Franchi, S., Bonomo, M.G., Salzano, G., Milan, S., and Bufo, S.A. (2018). Identification and antimicrobial activity of most representative secondary metabolites from different plant species. Chem. Biol. Technol. Agric., 5.","DOI":"10.1186\/s40538-018-0125-0"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Bishehsari, F., Engen, P.A., Preite, N.Z., Tuncil, Y.E., Naqib, A., Shaikh, M., and Keshavarzian, A. (2018). Dietary Fiber Treatment Corrects the Composition of Gut Microbiota, Promotes SCFA Production, and Suppresses Colon Carcinogenesis. Genes, 9.","DOI":"10.3390\/genes9020102"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1089\/jmf.2004.7.430","article-title":"Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals","volume":"7","author":"Hong","year":"2004","journal-title":"J. Med. Food"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Jamil, A., Rofida, S., Priyani, D., Nabila, W., and Wulandari, E. (2020, February 01). Phytochemical Screening and Antimicrobial Activity of Limonia acidissima Ethanol Extract against Microbes from Clinical Isolates. Available online: https:\/\/www.scitepress.org\/PublishedPapers\/2019\/91260\/91260.pdf.","DOI":"10.5220\/0009126001520156"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/0305-4179(94)90035-3","article-title":"Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture","volume":"20","author":"IHolder","year":"1994","journal-title":"Burns"},{"key":"ref_37","first-page":"1","article-title":"Package \u2018ggplot2\u2019","volume":"2","author":"Wickham","year":"2016","journal-title":"Creat. Elegant Data Vis. Using Gramm. Graphics. Version"},{"key":"ref_38","unstructured":"Wickham, H., and Wickham, M.H. (2020, February 01). Package Tidyverse. Easily Install Load \u2018Tidyverse\u2019. Available online: http:\/\/gwmodel.whu.edu.cn\/mirrors\/CRAN\/web\/packages\/tidyverse\/tidyverse.pdf."},{"key":"ref_39","unstructured":"Wilke, C.O., Wickham, H., and Wilke, M.C.O. (2020, February 01). Package \u2018cowplot\u2019. Streamlined Plot Theme Plot Annot. \u2018ggplot2\u2019. Available online: https:\/\/mirror.lyrahosting.com\/CRAN\/web\/packages\/cowplot\/cowplot.pdf."},{"key":"ref_40","unstructured":"Kassambara, A., and Kassambara, M.A. (Package \u2018ggpubr\u2019, 2020). Package \u2018ggpubr\u2019, R Package Version 0.1."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1007\/s00726-021-03051-2","article-title":"Proline metabolism and redox; maintaining a balance in health and disease","volume":"53","author":"Vettore","year":"2021","journal-title":"Amino Acids"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.micpath.2016.08.004","article-title":"Antimicrobial and enhancement of the antibiotic activity by phenolic compounds: Gallic acid, caffeic acid and pyrogallol","volume":"99","author":"Lima","year":"2016","journal-title":"Microb. Pathog."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1007\/s00253-012-4269-8","article-title":"Recent research on 3-phenyllactic acid, a broad-spectrum antimicrobial compound","volume":"95","author":"Mu","year":"2012","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_44","first-page":"54","article-title":"Synthesis, structural characterization and antimicrobial potency of anthranilic acid based Mn (II) Schiff base complex","volume":"4","author":"Ahmed","year":"2019","journal-title":"Chem. Res. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.copbio.2011.08.007","article-title":"Polyphenols as antimicrobial agents","volume":"32","author":"Daglia","year":"2012","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Morales-Mena, A., Mart\u00ednez-Gonz\u00e1lez, S., Teague, K.D., Graham, L.E., Se\u00f1as-Cuesta, R., Vuong, C.N., and Tellez-Isaias, G. (2020). Assessment of Fermented Soybean Meal on Salmonella typhimurium Infection in Neonatal Turkey Poults. Animals, 10.","DOI":"10.3390\/ani10101849"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"108235","DOI":"10.1016\/j.foodcont.2021.108235","article-title":"Influence of fermentation on the characteristics of Baltic Sea macroalgae, including microbial profile and trace element content","volume":"129","author":"Tolpeznikaite","year":"2021","journal-title":"Food Control"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1517\/17425255.2011.573478","article-title":"Evaluating the feasibility of lantibiotics as an alternative therapy against bacterial infections in humans","volume":"7","author":"Kuipers","year":"2011","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Perez, R.H., Zendo, T., and Sonomoto, K. (2018). Circular and Leaderless Bacteriocins: Biosynthesis, Mode of Action, Applications, and Prospects. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.02085"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/5\/891\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:35:48Z","timestamp":1760106948000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/5\/891"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,29]]},"references-count":49,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["microorganisms12050891"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12050891","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,29]]}}}