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Therefore, it is urgent to map new antigens or enhance the existing ones for future diagnostic techniques. Immunoinformatic tools are promising in the identification of new potential epitopes or antigen candidates. In this study, we evaluated peptides selected by epitope prediction for CVL serodiagnosis in ELISA assays. Ten B-cell epitopes were immunogenic in silico, but two peptides (peptides No. 45 and No. 48) showed the best performance in vitro. The selected peptides, both individually and in combination, were highly diagnostically accurate, with sensitivities ranging from 86.4% to 100% and with a specificity of approximately 90%. We observed that the combination of peptides showed better performance when compared to peptide alone, by detecting all asymptomatic dogs, showing lower cross-reactivity in sera from dogs with other canine infections, and did not detect vaccinated animals. Moreover, our data indicate the potential use of immunoinformatic tools associated with ELISA assays for the selection and evaluation of potential new targets, such as peptides, applied to the diagnosis of CVL.<\/jats:p>","DOI":"10.3390\/microorganisms12050906","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T08:42:57Z","timestamp":1714639377000},"page":"906","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Synthetic Peptides Selected by Immunoinformatics as Potential Tools for the Specific Diagnosis of Canine Visceral Leishmaniasis"],"prefix":"10.3390","volume":"12","author":[{"given":"Gabriel","family":"Moreira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3847-3949","authenticated-orcid":false,"given":"Rodrigo","family":"Maia","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nath\u00e1lia","family":"Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais","family":"Ostolin","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1434-7676","authenticated-orcid":false,"given":"Wendel","family":"Coura-Vital","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Escola de Farm\u00e1cia, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8705-7357","authenticated-orcid":false,"given":"Rodrigo","family":"Aguiar-Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Biotecnologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeronimo","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Grupo de Inform\u00e1tica de Biossistemas e Gen\u00f4mica, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Instituto Ren\u00e9 Rachou, Fiocruz Minas, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Resende","sequence":"additional","affiliation":[{"name":"Grupo de Inform\u00e1tica de Biossistemas e Gen\u00f4mica, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias da Sa\u00fade, Instituto Ren\u00e9 Rachou, Fiocruz Minas, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7591-5331","authenticated-orcid":false,"given":"Rory","family":"de Brito","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8123-4164","authenticated-orcid":false,"given":"Alexandre","family":"Reis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"},{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Escola de Farm\u00e1cia, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais, INCT-DT, Salvador 40296-710, BA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5281-3263","authenticated-orcid":false,"given":"Bruno","family":"Roatt","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunopatologia, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas, N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas\/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"},{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Instituto de Ci\u00eancias Exatas e Biol\u00f3gicas, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"961","DOI":"10.3855\/jidc.4310","article-title":"Diagnosis of leishmaniasis","volume":"8","author":"Elmahallawy","year":"2014","journal-title":"J. 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