{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:02:25Z","timestamp":1760144545430,"version":"build-2065373602"},"reference-count":21,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut Hospitalo-Universitaire (IHU) M\u00e9diterran\u00e9e Infection"},{"name":"French National Research Agency under the program \u201cInvestissements d\u2019avenir\u201d"},{"name":"reference ANR-10-IAHU-03"},{"name":"R\u00e9gion Provence Alpes C\u00f4te d\u2019Azur"},{"name":"European funding FEDER PRIMI"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Because data on the fungal gut community structure of African children are scarce, we aimed to describe it by reanalysing rRNA ITS1 and ITS2 metabarcoding data from a study designed to assess the influence of microbiota in malaria susceptibility in Malian children from the Dogon country. More specifically, we aimed to establish the core gut mycobiome and compare the gut fungal community structure of breastfed children, aged 0\u20132 years, with other age groups. Briefly, DNA was extracted from 296 children\u2019s stool samples. Both rRNA ITS1 and ITS2 genomic barcodes were amplified and subjected to Illumina MiSeq sequencing. The ITS2 barcode generated 1,975,320 reads and 532 operational taxonomic units (OTUs), while the ITS1 barcode generated 647,816 reads and 532 OTUs. The alpha diversity was significantly higher by using the ITS1 compared to the ITS2 barcode (p &lt; 0.05); but, regardless of the ITS barcode, we found no significant difference between breastfed children, aged 0\u20132 years, compared to the other age groups. The core gut mycobiome of the Malian children included Saccharomyces cerevisiae, Candida albicans, Pichia kudriavzevii, Malassezia restricta, Candida tropicalis and Aspergillus section Aspergillus, which were present in at least 50% of the 296 children. Further studies in other African countries are warranted to reach a global view of African children\u2019s core gut mycobiome.<\/jats:p>","DOI":"10.3390\/microorganisms12050926","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T11:39:42Z","timestamp":1714649982000},"page":"926","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Malian Children\u2019s Core Gut Mycobiome"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0919-1061","authenticated-orcid":false,"given":"Abdourahim","family":"Abdillah","sequence":"first","affiliation":[{"name":"IHU-M\u00e9diterran\u00e9e Infection, 13385 Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aly","family":"Kodio","sequence":"additional","affiliation":[{"name":"IHU-M\u00e9diterran\u00e9e Infection, 13385 Marseille, France"},{"name":"Malaria Research and Training Centre-International Center for Excellence in Research (MRTC-ICER), Department of Epidemiology of Parasitic Diseases, Faculty of Medicine and Dentistry, Universit\u00e9 des Sciences des Techniques et des Technologies de Bamako, Point G, Bamako BP 1805, Mali"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3293-5276","authenticated-orcid":false,"given":"St\u00e9phane","family":"Ranque","sequence":"additional","affiliation":[{"name":"IHU-M\u00e9diterran\u00e9e Infection, 13385 Marseille, France"},{"name":"AP-HM, RITMES, Aix-Marseille Universit\u00e9, 13005 Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2457","DOI":"10.1007\/s10096-017-3085-6","article-title":"Dysbiosis signature of mycobiota in colon polyp and colorectal cancer","volume":"36","author":"Gao","year":"2017","journal-title":"Eur. 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