{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:33:18Z","timestamp":1787067198498,"version":"build-2736575974"},"reference-count":33,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,11]],"date-time":"2024-05-11T00:00:00Z","timestamp":1715385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: Rapid diagnosis and identification of pathogens are pivotal for appropriate therapy of blood stream infections. The T2Bacteria\u00aePanel, a culture-independent assay for the detection of Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa in blood, was evaluated under real-world conditions as a point-of-care method including patients admitted to the internal medicine ward due to suspected blood stream infection. Methods: Patients were assigned to two groups (standard of care\u2014SOC vs. T2). In the SOC group 2 \u00d7 2 blood culture samples were collected, in the T2 group the T2Bacteria\u00aePanel was performed additionally for pathogen identification. Results: A total of 94 patients were included. Pathogens were detected in 19 of 50 patients (38%) in the T2 group compared to 16 of 44 patients (36.4%) in the SOC group. The median time until pathogen detection was significantly shorter in the T2 group (4.5 h vs. 60 h, p &lt; 0.001), as well as the time until targeted therapy (antibiotic with the narrowest spectrum and maximal effectiveness) (6.4 h vs. 42.2 h, p = 0.043). Conclusions: The implementation of the T2Bacteria\u00aePanel for patients with sepsis leads to an earlier targeted antimicrobial therapy resulting in earlier sufficient treatment and decreased excessive usage of broad-spectrum antimicrobials.<\/jats:p>","DOI":"10.3390\/microorganisms12050967","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T06:59:32Z","timestamp":1715756372000},"page":"967","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Point-of-Care Method T2Bacteria\u00aePanel Enables a More Sensitive and Rapid Diagnosis of Bacterial Blood Stream Infections and a Shorter Time until Targeted Therapy than Blood Culture"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0484-2240","authenticated-orcid":false,"given":"Tamara","family":"Clodi-Seitz","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine, Klinik Favoriten, 1100 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Baumgartner","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine, Klinik Favoriten, 1100 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Osteology, Klinik Favoriten, 1100 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Mader","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine, Klinik Favoriten, 1100 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4409-6855","authenticated-orcid":false,"given":"Julian","family":"Hind","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine, Klinik Favoriten, 1100 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Wenisch","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine, Klinik Favoriten, 1100 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Zoufaly","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine, Klinik Favoriten, 1100 Vienna, Austria"},{"name":"Faculty of Medicine, Sigmund Freud University Vienna, 1020 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Presterl","sequence":"additional","affiliation":[{"name":"Department of Hospital Epidemiology and Infection Control, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1128\/CMR.00053-14","article-title":"Effectiveness of Practices To Increase Timeliness of Providing Targeted Therapy for Inpatients with Bloodstream Infections: A Laboratory Medicine Best Practices Systematic Review and Meta-analysis","volume":"29","author":"Buehler","year":"2016","journal-title":"Clin. 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