{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T16:42:37Z","timestamp":1781800957750,"version":"3.54.5"},"reference-count":43,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T00:00:00Z","timestamp":1716336000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["302222\/2016-2"],"award-info":[{"award-number":["302222\/2016-2"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["423836\/2018-8"],"award-info":[{"award-number":["423836\/2018-8"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["46360.673.28686.05082021"],"award-info":[{"award-number":["46360.673.28686.05082021"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["54758.924.28686.25102022"],"award-info":[{"award-number":["54758.924.28686.25102022"]}]},{"name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Estado da Para\u00edba (FAPESQ)","award":["302222\/2016-2"],"award-info":[{"award-number":["302222\/2016-2"]}]},{"name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Estado da Para\u00edba (FAPESQ)","award":["423836\/2018-8"],"award-info":[{"award-number":["423836\/2018-8"]}]},{"name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Estado da Para\u00edba (FAPESQ)","award":["46360.673.28686.05082021"],"award-info":[{"award-number":["46360.673.28686.05082021"]}]},{"name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Estado da Para\u00edba (FAPESQ)","award":["54758.924.28686.25102022"],"award-info":[{"award-number":["54758.924.28686.25102022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Leptospirosis is an infectious disease that affects domestic animals, wild animals, and humans. It represents a public health problem and has an important economic impact on livestock. This study aims to investigate the importance of genital and transplacental infection in the epidemiology of leptospirosis in cows maintained in Caatinga biome conditions, Northeastern Brazil, as well as reporting organs colonized by Leptospira spp. in embryos and fetuses. Blood, urinary tract (urine, bladder, and kidney), and reproductive tract (vaginal fluid, uterus, uterine tube, ovary, and placenta) samples were collected from 15 slaughtered pregnant cows. Two embryos and 13 fetuses were sampled. Central nervous system and choroid ovoid samples were collected from embryos. Blood, central nervous system, lung, peritoneal liquid, abomasal content, liver, spleen, urine, bladder, kidney, and reproductive system samples were collected from fetuses. Diagnostic methods included the microscopic agglutination test (MAT) using a collection of 24 serovars belonging to 17 different pathogenic serogroups of five species as antigens, as well as polymerase chain reaction (PCR). Anti-Leptospira spp. antibodies were found in 9 cows (60%), while 13 cows (86.67%) had at least one organ or urine with leptospiral DNA. No fetus was seroreactive. Among the embryos and fetuses, 13 (86.67%) presented leptospiral DNA, proving a high frequency of transplacental infection (100%). For cows, the most frequent biological materials regarding Leptospira spp. DNA detection were placenta (13 out of 15 samples; 86.7%), uterus (10 out of 15 samples; 66.7%), and vaginal fluid (5 out of 15 samples; 33.3%), while, for fetuses\/embryos, the most frequent PCR-positive samples were choroid ovoid (1\/2; 50%), spleen (6\/13; 46.2%), kidney (5\/13; 38.5%), and central nervous system (5\/15; 33.3%). Sequenced samples based on the LipL32 gene presented 99% similarity with L. borgpetersenii. The results indicate that transplacental infection is an efficient way of spreading Leptospira spp. in cows maintained in Caatinga biome conditions. Therefore, prevention and control strategies must include actions that interrupt transmission through this alternative route.<\/jats:p>","DOI":"10.3390\/microorganisms12061044","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T07:56:11Z","timestamp":1716364571000},"page":"1044","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Role of Transplacental Infection in Leptospira spp. Epidemiology in Cattle in Caatinga Biome, Brazil"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7332-8504","authenticated-orcid":false,"given":"Nathanael Nat\u00e9rcio","family":"da Costa Barnab\u00e9","sequence":"first","affiliation":[{"name":"Centro de Sa\u00fade Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universit\u00e1ria, s\/n, Santa Cec\u00edlia, Patos 58708110, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Rodrigues","family":"Soares","sequence":"additional","affiliation":[{"name":"Centro de Sa\u00fade Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universit\u00e1ria, s\/n, Santa Cec\u00edlia, Patos 58708110, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deivyson Kelvis Silva","family":"Barros","sequence":"additional","affiliation":[{"name":"Centro de Sa\u00fade Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universit\u00e1ria, s\/n, Santa Cec\u00edlia, Patos 58708110, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9153-1485","authenticated-orcid":false,"given":"Jo\u00e3o Pessoa","family":"Ara\u00fajo J\u00fanior","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias, Departamento de Microbiologia e Imunologia, Universidade Estadual Paulista (Unesp), Av. Prof. M\u00e1rio Rubens Guimar\u00e3es Montenegro, s\/n, Botucatu 18618687, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3841-2479","authenticated-orcid":false,"given":"Camila Dantas","family":"Malossi","sequence":"additional","affiliation":[{"name":"Instituto de Bioci\u00eancias, Departamento de Microbiologia e Imunologia, Universidade Estadual Paulista (Unesp), Av. Prof. M\u00e1rio Rubens Guimar\u00e3es Montenegro, s\/n, Botucatu 18618687, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luana Cristiny","family":"Rodrigues Silva","sequence":"additional","affiliation":[{"name":"Centro de Sa\u00fade Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universit\u00e1ria, s\/n, Santa Cec\u00edlia, Patos 58708110, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1862-6517","authenticated-orcid":false,"given":"Arthur Willian de Lima","family":"Brasil","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias Agr\u00e1rias (CCA), Universidade Federal da Para\u00edba (UFPB), Rodovia BR 079, Km 02, Areia 58397000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego Figueiredo","family":"da Costa","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias Agr\u00e1rias (CCA), Universidade Federal da Para\u00edba (UFPB), Rodovia BR 079, Km 02, Areia 58397000, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1784-7481","authenticated-orcid":false,"given":"Severino Silvano dos Santos","family":"Higino","sequence":"additional","affiliation":[{"name":"Centro de Sa\u00fade Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universit\u00e1ria, s\/n, Santa Cec\u00edlia, Patos 58708110, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina de Sousa Am\u00e9rico Batista","family":"Santos","sequence":"additional","affiliation":[{"name":"Centro de Sa\u00fade Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. Universit\u00e1ria, s\/n, Santa Cec\u00edlia, Patos 58708110, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1777-7348","authenticated-orcid":false,"given":"S\u00e9rgio Santos","family":"de Azevedo","sequence":"additional","affiliation":[{"name":"Centro de Sa\u00fade Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Av. 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