{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:03:39Z","timestamp":1785769419319,"version":"3.56.0"},"reference-count":36,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fleming Fund Fellowship Scheme through the London School of Hygiene and Tropical Medicine and the University of Ghana","award":["RZ07"],"award-info":[{"award-number":["RZ07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Escherichia coli, a member of the commensal intestinal microbiota, is a significant aetiology of urinary tract infections (UTIs) and has a propensity for acquiring multidrug resistance characteristics, such as extended-spectrum beta-lactamases (ESBLs). Despite the increase in the incidence of ESBL-producing E. coli infections in sub-Saharan Africa, routine ESBL detection in Ghana is often absent, and molecular data on ESBL genotypes is scarce. Eleven ESBL-producing E. coli recovered from mid-stream urine samples were subjected to antimicrobial susceptibility testing and whole-genome sequence analyses. All isolates exhibited multidrug resistance, demonstrating phenotypic resistance to third-generation cephalosporins, such as cefotaxime, ceftazidime, and cefpodoxime. Three isolates demonstrated resistance to norfloxacin (a fluoroquinolone), and one isolate demonstrated intermediate resistance to ertapenem (a carbapenem). Analysis of the draft genomes identified multiple antimicrobial resistance genes including ESBL genotypes blaTEM-1B\/TEM-190 (6\/11 and 1\/11, respectively), blaCTX-M-15\/CTX-M-3 (7\/11 and 1\/11) and blaOXA-1\/OXA-181 (3\/11 and 1\/11). The strains belong to 10 different serotypes and 10 different multilocus sequence types. This study provides information on phenotypic resistance in 11 ESBL E. coli from Ghana and AMR genotypes within their genomes.<\/jats:p>","DOI":"10.3390\/microorganisms12061139","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T08:43:54Z","timestamp":1717577034000},"page":"1139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genome Sequences of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Recovered from Mid-Stream Urine Samples in Accra, Ghana"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4491-6902","authenticated-orcid":false,"given":"Nicholas T. K. D.","family":"Dayie","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, Accra P.O. Box KB 4236, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly","family":"Egyir","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra P.O. Box LG 581, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia","family":"Amoa-Owusu","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra P.O. Box LG 581, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Owusu-Nyantakyi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra P.O. Box LG 581, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bright","family":"Adu","sequence":"additional","affiliation":[{"name":"Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra P.O. Box LG 581, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0286-3638","authenticated-orcid":false,"given":"Fleischer C. N.","family":"Kotey","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, Accra P.O. Box KB 4236, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric S.","family":"Donkor","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, Accra P.O. Box KB 4236, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2402-6630","authenticated-orcid":false,"given":"Richard A.","family":"Stabler","sequence":"additional","affiliation":[{"name":"Department of Infection Biology, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3945","DOI":"10.2147\/IDR.S269033","article-title":"Risk Factors of Extended-Spectrum Beta-Lactamases-Producing Escherichia coli Community Acquired Urinary Tract Infections: A Systematic Review","volume":"13","author":"Larramendy","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Aminov, R.I. (2011). Horizontal Gene Exchange in Environmental Microbiota. Front. 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