{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T20:40:28Z","timestamp":1781124028586,"version":"3.54.1"},"reference-count":119,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T00:00:00Z","timestamp":1718236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004385","name":"Ghent University","doi-asserted-by":"publisher","award":["F2018\/IOF-ConcepTT\/410"],"award-info":[{"award-number":["F2018\/IOF-ConcepTT\/410"]}],"id":[{"id":"10.13039\/501100004385","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004385","name":"Ghent University","doi-asserted-by":"publisher","award":["BOF20\/DOC\/046"],"award-info":[{"award-number":["BOF20\/DOC\/046"]}],"id":[{"id":"10.13039\/501100004385","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Chlamydia (C.) trachomatis, a leading cause of sexually transmitted infections (STIs) worldwide, continues to be a significant public health concern. The majority of infections are asymptomatic and, when left untreated, severe sequelae such as infertility and chronic pelvic pain can occur. Despite decades of research, an effective vaccine remains elusive. This review focuses on the potential of Major Outer Membrane Protein (MOMP)-derived constructs as promising candidates for C. trachomatis vaccination. MOMP, the most abundant protein in the outer membrane of C. trachomatis, has been a focal point of vaccine research over the years due to its antigenic properties. To overcome issues associated with the use of full MOMP as a vaccine antigen, derivative constructs have been studied. As these constructs are often not sufficiently immunogenic, antigen delivery systems or accompanying adjuvants are required. Additionally, several immunization routes have been explored with these MOMP-derived vaccine antigens, and determining the optimal route remains an ongoing area of research. Future directions and challenges in the field of C. trachomatis vaccination are discussed.<\/jats:p>","DOI":"10.3390\/microorganisms12061196","type":"journal-article","created":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T10:41:03Z","timestamp":1718275263000},"page":"1196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Advances in Chlamydia trachomatis Vaccination: Unveiling the Potential of Major Outer Membrane Protein Derivative Constructs"],"prefix":"10.3390","volume":"12","author":[{"given":"Celien","family":"Kiekens","sequence":"first","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Servaas A.","family":"Morr\u00e9","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, GROW School for Oncology and Reproduction, Maastricht University, 6229 ER Maastricht, The Netherlands"},{"name":"Microbe&Lab BV, 1105 AG Amsterdam, The Netherlands"},{"name":"Dutch <i>Chlamydia trachomatis<\/i> Reference Laboratory, Department of Medical Microbiology, Faculty of Health, Medicine & Life Sciences, Maastricht University, 6229 ER Maastricht, The Netherlands"},{"name":"Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad 211007, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy","family":"Vanrompay","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1016\/j.vaccine.2021.03.043","article-title":"Chlamydia trachomatis: Cell Biology, Immunology and Vaccination","volume":"39","author":"Murray","year":"2021","journal-title":"Vaccine"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Phillips, S., Quigley, B.L., and Timms, P. (2019). Seventy Years of Chlamydia Vaccine Research\u2014Limitations of the Past and Directions for the Future. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00070"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Grygiel-G\u00f3rniak, B., and Folga, B.A. (2023). Chlamydia trachomatis\u2014An Emerging Old Entity?. Microorganisms, 11.","DOI":"10.3390\/microorganisms11051283"},{"key":"ref_4","unstructured":"World Health Organization (2023). Chlamydia, WHO."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1097\/OLQ.0000000000000175","article-title":"Cross-Sectional Study of Genital, Rectal, and Pharyngeal Chlamydia and Gonorrhea in Women in Rural South Africa","volume":"41","author":"Peters","year":"2014","journal-title":"Sex. Transm. Dis."},{"key":"ref_6","unstructured":"CDC (2024). Sexually Transmitted Disease Surveillance 2021."},{"key":"ref_7","unstructured":"ECDC (2024). Chlamydia\u2014Annual Epidemiological Report for 2022."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/j.bpobgyn.2006.06.003","article-title":"A Review on Infection with Chlamydia trachomatis","volume":"20","author":"Manavi","year":"2006","journal-title":"Best. Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Rodrigues, R., Sousa, C., and Vale, N. (2022). Chlamydia trachomatis as a Current Health Problem: Challenges and Opportunities. Diagnostics, 12.","DOI":"10.3390\/diagnostics12081795"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1093\/humupd\/5.5.433","article-title":"Chlamydia trachomatis: Impact on Human Reproduction","volume":"5","author":"Paavonen","year":"1999","journal-title":"Hum. Reprod. Update"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1093\/infdis\/jiab610","article-title":"Problems with Understanding Chlamydia trachomatis Immunology","volume":"225","author":"Brunham","year":"2022","journal-title":"J. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1016\/j.vaccine.2013.08.032","article-title":"Current Barriers, Challenges and Opportunities for the Development of Effective STI Vaccines: Point of View of Vaccine Producers, Biotech Companies and Funding Agencies","volume":"32","author":"Dodet","year":"2014","journal-title":"Vaccine"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1080\/14760584.2022.2117694","article-title":"Chlamydia trachomatis Vaccine Development\u2014A View on the Current Challenges and How to Move Forward","volume":"21","author":"Borges","year":"2022","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1362","DOI":"10.1128\/IAI.01244-13","article-title":"Hidden in Plain Sight: Chlamydial Gastrointestinal Infection and Its Relevance to Persistence in Human Genital Infection","volume":"82","author":"Rank","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/ar382","article-title":"Persistent Chlamydiae and Chronic Arthritis","volume":"4","author":"Villareal","year":"2002","journal-title":"Arthritis Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1080\/14760584.2021.1899817","article-title":"Chlamydia trachomatis Vaccines for Genital Infections: Where Are We and How Far Is There to Go?","volume":"20","author":"Darville","year":"2021","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_17","first-page":"e14702","article-title":"Evaluation of the Role of Chlamydia trachomatis in Primary Male Infertility","volume":"75","author":"Sorour","year":"2021","journal-title":"Int. J. Clin. Pract."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1177\/0956462417735245","article-title":"The Impact of Chlamydia trachomatis Infection on Sperm Parameters and Male Fertility: A Comprehensive Study","volume":"29","author":"Moazenchi","year":"2018","journal-title":"Int. J. STD AIDS"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7346","DOI":"10.1016\/j.vaccine.2017.10.075","article-title":"National Institute of Allergy and Infectious Diseases Workshop Report: \u201cChlamydia Vaccines: The Way Forward\u201d","volume":"37","author":"Zhong","year":"2019","journal-title":"Vaccine"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/S0140-6736(18)31816-6","article-title":"Population Effectiveness of Opportunistic Chlamydia Testing in Primary Care in Australia: A Cluster-Randomised Controlled Trial","volume":"392","author":"Hocking","year":"2018","journal-title":"Lancet"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1080\/14760584.2022.2061461","article-title":"Chlamydia trachomatis: Quest for an Eye-Opening Vaccine Breakthrough","volume":"21","author":"Chavda","year":"2022","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Hou, C., Jin, Y., Wu, H., Li, P., Liu, L., Zheng, K., and Wang, C. (2022). Alternative Strategies for Chlamydia Treatment: Promising Non-Antibiotic Approaches. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.987662"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1093\/infdis\/jiv137","article-title":"Protection Against Chlamydia trachomatis Infection and Upper Genital Tract Pathological Changes by Vaccine-Promoted Neutralizing Antibodies Directed to the VD4 of the Major Outer Membrane Protein","volume":"212","author":"Olsen","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"170194","DOI":"10.3389\/fimmu.2015.00628","article-title":"Intramuscular Priming and Intranasal Boosting Induce Strong Genital Immunity through Secretory IgA in Minipigs Infected with Chlamydia trachomatis","volume":"6","author":"Lorenzen","year":"2015","journal-title":"Front. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.3389\/fimmu.2018.01981","article-title":"An Adaptive Chlamydia trachomatis-Specific IFN-\u03b3-Producing CD4+ T Cell Response Is Associated With Protection Against Chlamydia Reinfection in Women","volume":"9","author":"Bakshi","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Yount, K.S., Kollipara, A., Liu, C., Zheng, X., O\u2019Connell, C.M., Bagwell, B., Wiesenfeld, H.C., Hillier, S.L., and Darville, T. (2023). Unique T Cell Signatures Are Associated with Reduced Chlamydia trachomatis Reinfection in a Highly Exposed Cohort. bioRxiv.","DOI":"10.1101\/2023.08.02.551709"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kuczkowska, K., Myrbr\u00e5ten, I., \u00d8verland, L., Eijsink, V.G.H., Follmann, F., Mathiesen, G., and Dietrich, J. (2017). Lactobacillus plantarum Producing a Chlamydia trachomatis Antigen Induces a Specific IgA Response after Mucosal Booster Immunization. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0176401"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"222004","DOI":"10.3389\/fimmu.2017.00569","article-title":"Simultaneous Subcutaneous and Intranasal Administration of a CAF01-Adjuvanted Chlamydia Vaccine Elicits Elevated IgA and Protective Th1\/Th17 Responses in the Genital Tract","volume":"8","author":"Wern","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"aaa8205","DOI":"10.1126\/science.aaa8205","article-title":"A Mucosal Vaccine against Chlamydia trachomatis Generates Two Waves of Protective Memory T Cells","volume":"348","author":"Stary","year":"2015","journal-title":"Science"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/infdis\/jiz522","article-title":"Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development","volume":"221","author":"Karunakaran","year":"2020","journal-title":"J. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1051\/vetres:2006010","article-title":"The IgA System: A Comparison of Structure and Function in Different Species","volume":"37","author":"Snoeck","year":"2006","journal-title":"Vet. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1111\/j.0105-2896.2005.00290.x","article-title":"Mucosal Immunoglobulins","volume":"206","author":"Woof","year":"2005","journal-title":"Immunol. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1016\/j.vaccine.2013.08.020","article-title":"Development Status and Future Prospects for a Vaccine against Chlamydia trachomatis Infection","volume":"32","author":"Hafner","year":"2014","journal-title":"Vaccine"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1057375","DOI":"10.3389\/fimmu.2022.1057375","article-title":"Multi-Component Prime-Boost Chlamydia trachomatis Vaccination Regimes Induce Antibody and T Cell Responses and Accelerate Clearance of Infection in a Non-Human Primate Model","volume":"13","author":"Lorenzen","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/icb.2015.79","article-title":"A Multi-Subunit Chlamydia Vaccine Inducing Neutralizing Antibodies and Strong IFN-\u03b3 + CMI Responses Protects against a Genital Infection in Minipigs","volume":"94","author":"Olsen","year":"2016","journal-title":"Immunol. Cell Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2102508","DOI":"10.1002\/adhm.202102508","article-title":"Sublingual Boosting with a Novel Mucoadhesive Thermogelling Hydrogel Following Parenteral CAF01 Priming as a Strategy Against Chlamydia trachomatis","volume":"11","author":"Pedersen","year":"2022","journal-title":"Adv. Healthc. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"15121","DOI":"10.1074\/jbc.M117.784561","article-title":"Cell-Free Production of a Functional Oligomeric Form of a Chlamydia Major Outer-Membrane Protein (MOMP) for Vaccine Development","volume":"292","author":"He","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.jconrel.2022.11.015","article-title":"Oral Vaccination Using Microdevices to Deliver \u03b1-GalCer Adjuvanted Vaccine Afford a Mucosal Immune Response","volume":"353","author":"Carlsen","year":"2023","journal-title":"J. Control. Release"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"790463","DOI":"10.3389\/fimmu.2021.790463","article-title":"Th1\/Th17 T Cell Tissue-Resident Immunity Increases Protection, But Is Not Required in a Vaccine Strategy Against Genital Infection With Chlamydia trachomatis","volume":"12","author":"Nguyen","year":"2021","journal-title":"Front. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.jconrel.2017.12.003","article-title":"A Strong Adjuvant Based on Glycol-Chitosan-Coated Lipid-Polymer Hybrid Nanoparticles Potentiates Mucosal Immune Responses against the Recombinant Chlamydia trachomatis Fusion Antigen CTH522","volume":"271","author":"Rose","year":"2018","journal-title":"J. Control. Release"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s40794-022-00176-4","article-title":"Vaccines Platforms and COVID-19: What You Need to Know","volume":"8","year":"2022","journal-title":"Trop. Dis. Travel Med. Vaccines"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1093\/abbs\/gmu016","article-title":"A Multi-Epitope Vaccine Based on Chlamydia trachomatis Major Outer Membrane Protein Induces Specific Immunity in Mice","volume":"46","author":"Tu","year":"2014","journal-title":"Acta Biochim. Biophys. Sin."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Findlay, H.E., McClafferty, H., and Ashley, R.H. (2005). Surface Expression, Single-Channel Analysis and Membrane Topology of Recombinant Chlamydia trachomatis Major Outer Membrane Protein. BMC Microbiol., 5.","DOI":"10.1186\/1471-2180-5-5"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1073\/pnas.85.11.4000","article-title":"Mapping Antigenic Domains Expressed by Chlamydia trachomatis Major Outer Membrane Protein Genes","volume":"85","author":"Baehr","year":"1988","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1084\/jem.167.3.817","article-title":"High-Resolution Mapping of Serovar-Specific and Common Antigenic Determinants of the Major Outer Membrane Protein of Chlamydia trachomatis","volume":"167","author":"Stephens","year":"1988","journal-title":"J. Exp. Med."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein Structure Prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"D368","DOI":"10.1093\/nar\/gkad1011","article-title":"AlphaFold Protein Structure Database in 2024: Providing Structure Coverage for over 214 Million Protein Sequences","volume":"52","author":"Varadi","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8153","DOI":"10.1128\/IAI.73.12.8153-8160.2005","article-title":"Vaccination with the Chlamydia trachomatis Major Outer Membrane Protein Can Elicit an Immune Response as Protective as That Resulting from Inoculation with Live Bacteria","volume":"73","author":"Pal","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1128\/IAI.72.2.1019-1028.2004","article-title":"Transcutaneous Immunization with Combined Cholera Toxin and CpG Adjuvant Protects against Chlamydia Muridarum Genital Tract Infection","volume":"72","author":"Berry","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1038\/s41541-020-00239-7","article-title":"Vaccination with the Recombinant Major Outer Membrane Protein Elicits Long-Term Protection in Mice against Vaginal Shedding and Infertility Following a Chlamydia Muridarum Genital Challenge","volume":"5","author":"Pal","year":"2020","journal-title":"NPJ Vaccines"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6240","DOI":"10.1128\/IAI.69.10.6240-6247.2001","article-title":"Immunization with the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein Can Elicit a Protective Immune Response against a Genital Challenge","volume":"69","author":"Pal","year":"2001","journal-title":"Infect. Immun."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4107","DOI":"10.1007\/s00253-014-5517-x","article-title":"Hepatitis B Virus Surface Antigen as Delivery Vector Can Enhance Chlamydia trachomatis MOMP Multi-Epitope Immune Response in Mice","volume":"98","author":"Zhu","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1172\/JCI119109","article-title":"An N-Linked High-Mannose Type Oligosaccharide, Expressed at the Major Outer Membrane Protein of Chlamydia trachomatis, Mediates Attachment and Infectivity of the Microorganism to HeLa Cells","volume":"98","author":"Kuo","year":"1996","journal-title":"J. Clin. Investig."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8063","DOI":"10.4049\/jimmunol.0804375","article-title":"Chlamydia trachomatis Native Major Outer Membrane Protein Induces Partial Protection in Nonhuman Primates: Implication for a Trachoma Transmission-Blocking Vaccine","volume":"182","author":"Kari","year":"2009","journal-title":"J. Immunol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.1073\/pnas.0508983103","article-title":"Chlamydia trachomatis Polymorphic Membrane Protein D Is a Species-Common Pan-Neutralizing Antigen","volume":"103","author":"Crane","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S0952-7915(00)00237-5","article-title":"Epitope Clusters in the Major Outer Membrane Protein of Chlamydia trachomatis","volume":"13","author":"Kim","year":"2001","journal-title":"Curr. Opin. Immunol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1016\/j.xphs.2018.02.005","article-title":"Unusual Self-Assembly of the Recombinant Chlamydia trachomatis Major Outer Membrane Protein\u2013Based Fusion Antigen CTH522 Into Protein Nanoparticles","volume":"107","author":"Rose","year":"2018","journal-title":"J. Pharm. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Collar, A.L., Linville, A.C., Core, S.B., and Frietze, K.M. (2022). Epitope-Based Vaccines against the Chlamydia trachomatis Major Outer Membrane Protein Variable Domain 4 Elicit Protection in Mice. Vaccines, 10.","DOI":"10.3390\/vaccines10060875"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2672","DOI":"10.1016\/j.vaccine.2010.12.132","article-title":"Protective Immunity against Chlamydia trachomatis Genital Infection Induced by a Vaccine Based on the Major Outer Membrane Multi-Epitope Human Papillomavirus Major Capsid Protein L1","volume":"29","author":"Xu","year":"2011","journal-title":"Vaccine"},{"key":"ref_60","first-page":"942","article-title":"The Cellular Immune Response Produced in BALB\/c Mice Immunized with HPV6b L1\/Ct MOMP Multi-Epitope Chimeric DNA","volume":"30","author":"Shi","year":"2010","journal-title":"Chin. J. Microbiol. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"33241","DOI":"10.18632\/oncotarget.16601","article-title":"DNA Plasmid Vaccine Carrying Chlamydia trachomatis (Ct) Major Outer Membrane and Human Papillomavirus 16L2 Proteins for Anti-Ct Infection","volume":"8","author":"Wang","year":"2017","journal-title":"Oncotarget"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jconrel.2019.04.043","article-title":"Effects of Cationic Adjuvant Formulation Particle Type, Fluidity and Immunomodulators on Delivery and Immunogenicity of SaRNA","volume":"304","author":"Blakney","year":"2019","journal-title":"J. Control. Release"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"575","DOI":"10.4049\/jimmunol.138.2.575","article-title":"Protective Monoclonal Antibodies Recognize Epitopes Located on the Major Outer Membrane Protein of Chlamydia trachomatis","volume":"138","author":"Zhang","year":"1987","journal-title":"J. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1128\/iai.57.2.636-638.1989","article-title":"Protective Monoclonal Antibodies to Chlamydia trachomatis Serovar- and Serogroup-Specific Major Outer Membrane Protein Determinants","volume":"57","author":"Zhang","year":"1989","journal-title":"Infect. Immun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1128\/IAI.68.3.1719-1723.2000","article-title":"T-Cell Epitopes in Variable Segments of Chlamydia trachomatis Major Outer Membrane Protein Elicit Serovar-Specific Immune Responses in Infected Humans","volume":"68","author":"Ortiz","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4554","DOI":"10.4049\/jimmunol.157.10.4554","article-title":"Chlamydia trachomatis Major Outer Membrane Protein (MOMP) Epitopes That Activate HLA Class Il-Restricted T Cells from Infected Humans","volume":"157","author":"Ortiz","year":"1996","journal-title":"J. Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1128\/iai.7.3.356-360.1973","article-title":"A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lympho-Granuloma Venereum Organisms","volume":"7","author":"Wang","year":"1973","journal-title":"Infect. Immun."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1093\/infdis\/152.4.791","article-title":"Immunotyping of Chlamydia trachomatis with Monoclonal Antibodies","volume":"152","author":"Wang","year":"1985","journal-title":"J. Infect. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1128\/iai.57.2.487-494.1989","article-title":"Cloning and Characterization of a Chlamydia trachomatis L3 DNA Fragment That Codes for an Antigenic Region of the Major Outer Membrane Protein and Specifically Hybridizes to the C- and C-Related-Complex Serovars","volume":"57","author":"Carlson","year":"1989","journal-title":"Infect. Immun."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/j.cmi.2022.02.013","article-title":"Call for Consensus in Chlamydia trachomatis Nomenclature: Moving from Biovars, Serovars, and Serotypes to Genovariants and Genotypes","volume":"28","author":"Pannekoek","year":"2022","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_71","first-page":"218","article-title":"Chlamydia trachomatis Antigens: Role in Immunity and Pathogenesis","volume":"3","author":"Brunham","year":"1994","journal-title":"Infect. Agents Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.1016\/j.vaccine.2017.04.031","article-title":"Evaluation of Tandem Chlamydia trachomatis MOMP Multi-Epitopes Vaccine in BALB\/c Mice Model","volume":"35","author":"Jiang","year":"2017","journal-title":"Vaccine"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"43281","DOI":"10.18632\/oncotarget.6533","article-title":"Hepatitis B Virus Core Antigen as a Carrier for Chlamydia trachomatis MOMP Multi-Epitope Peptide Enhances Protection against Genital Chlamydial Infection","volume":"6","author":"Jiang","year":"2015","journal-title":"Oncotarget"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Aslam, S., Ahmad, S., Noor, F., Ashfaq, U.A., Shahid, F., Rehman, A., Ul Qamar, M.T., Alatawi, E.A., Alshabrmi, F.M., and Allemailem, K.S. (2021). Designing a Multi-Epitope Vaccine against Chlamydia trachomatis by Employing Integrated Core Proteomics, Immuno-Informatics and in Silico Approaches. Biology, 10.","DOI":"10.3390\/biology10100997"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/j.1365-2567.2007.02608.x","article-title":"Live-Attenuated Influenza Viruses as Delivery Vectors for Chlamydia Vaccines","volume":"122","author":"He","year":"2007","journal-title":"Immunology"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1086\/590670","article-title":"Liposome Delivery of Chlamydia Muridarum Major Outer Membrane Protein Primes a Th1 Response That Protects against Genital Chlamydial Infection in a Mouse Model","volume":"198","author":"Hansen","year":"2008","journal-title":"J. Infect. Dis."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"3074","DOI":"10.1128\/IAI.68.6.3074-3078.2000","article-title":"Priming with Chlamydia trachomatis Major Outer Membrane Protein (MOMP) DNA Followed by MOMP ISCOM Boosting Enhances Protection and Is Associated with Increased Immunoglobulin A and Th1 Cellular Immune Responses","volume":"68","author":"Yang","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"3909","DOI":"10.1128\/iai.58.12.3909-3913.1990","article-title":"Immunologic Characterization of a Cloned Fragment Containing the Species-Specific Epitope from the Major Outer Membrane Protein of Chlamydia trachomatis","volume":"58","author":"Toye","year":"1990","journal-title":"Infect. Immun."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"3428","DOI":"10.1128\/iai.60.8.3428-3432.1992","article-title":"Characterization of the Humoral Response Induced by a Peptide Corresponding to Variable Domain IV of the Major Outer Membrane Protein of Chlamydia trachomatis Serovar E","volume":"60","author":"Cheng","year":"1992","journal-title":"Infect. Immun."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/0264-410X(93)90080-H","article-title":"Immunogenicity of a Synthetic Oligopeptide Corresponding to Antigenically Common T-Helper and B-Cell Neutralizing Epitopes of the Major Outer Membrane Protein of Chlamydia trachomatis","volume":"11","author":"Su","year":"1993","journal-title":"Vaccine"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1099\/00221287-137-7-1557","article-title":"Chlamydia trachomatis Major Outer Membrane Protein Epitopes Expressed as Fusions with LamB in an Attenuated AroA Strain of Salmonella Typhimurium; Their Application as Potential Immunogens","volume":"137","author":"Hayes","year":"1991","journal-title":"J. Gen. Microbiol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1128\/CDLI.6.3.356-363.1999","article-title":"Immunization with a Peptide Corresponding to Chlamydial Heat Shock Protein 60 Increases the Humoral Immune Response in C3H Mice to a Peptide Representing Variable Domain 4 of the Major Outer Membrane Protein of Chlamydia trachomatis","volume":"6","author":"Motin","year":"1999","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"4406","DOI":"10.1128\/iai.61.10.4406-4414.1993","article-title":"A Poliovirus Hybrid Expressing a Neutralization Epitope from the Major Outer Membrane Protein of Chlamydia trachomatis Is Highly Immunogenic","volume":"61","author":"Murdin","year":"1993","journal-title":"Infect. Immun."},{"key":"ref_84","first-page":"8","article-title":"A Peptide of Chlamydia trachomatis Shown to Be a Primary T-Cell Epitope in Vitro Induces Cell-Mediated Immunity in Vivo","volume":"85","author":"Knight","year":"1995","journal-title":"Immunology"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/j.nano.2014.02.009","article-title":"Poly(Lactic Acid)-Poly(Ethylene Glycol) Nanoparticles Provide Sustained Delivery of a Chlamydia trachomatis Recombinant MOMP Peptide and Potentiate Systemic Adaptive Immune Responses in Mice","volume":"10","author":"Dixit","year":"2014","journal-title":"Nanomedicine"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"138","DOI":"10.4236\/wjv.2011.14014","article-title":"A Peptide Containing T-Cell Epitopes of Chlamydia trachomatis Recombinant MOMP Induces Systemic and Mucosal Antibody Responses in Mice","volume":"01","author":"Taha","year":"2011","journal-title":"World J. Vaccines"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"400511","DOI":"10.3389\/fimmu.2018.02369","article-title":"The Chlamydia M278 Major Outer Membrane Peptide Encapsulated in the Poly(Lactic Acid)-Poly(Ethylene Glycol) Nanoparticulate Self-Adjuvanting Delivery System Protects Mice against a Chlamydia Muridarumgenital Tract Challenge by Stimulating Robust Systemic and Local Mucosal Immune Responses","volume":"9","author":"Verma","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1038\/s41541-021-00312-9","article-title":"A Chlamydia trachomatis VD1-MOMP Vaccine Elicits Cross-Neutralizing and Protective Antibodies against C\/C-Related Complex Serovars","volume":"6","author":"Olsen","year":"2021","journal-title":"NPJ Vaccines"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1099\/00221287-138-8-1707","article-title":"Heterotypic Protection of Mice against Chlamydial Salpingitis and Colonization of the Lower Genital Tract with a Human Serovar F Isolate of Chlamydia trachomatis by Prior Immunization with Recombinant Serovar L1 Major Outer-Membrane Protein","volume":"138","author":"Tuffrey","year":"1992","journal-title":"J. Gen. Microbiol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/0264-410X(94)90317-4","article-title":"Analysis of the Humoral Response Elicited in Mice by a Chimeric Peptide Representing Variable Segments I and IV of the Major Outer Membrane Protein of Chlamydia trachomatis","volume":"12","author":"Qu","year":"1994","journal-title":"Vaccine"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/0161-5890(95)00157-3","article-title":"The Effect of Orientation within a Chimeric Peptide of the Immunogenicity of Chlamydia trachomatis Epitopes","volume":"33","author":"Peterson","year":"1996","journal-title":"Mol. Immunol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1111\/apm.12605","article-title":"Protection against Genital Tract Chlamydia trachomatis Infection Following Intranasal Immunization with a Novel Recombinant MOMP VS2\/4 Antigen","volume":"124","author":"Hadad","year":"2016","journal-title":"APMIS"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1016\/0264-410X(95)00017-U","article-title":"Protective Efficacy of a Parenterally Administered MOMP-Derived Synthetic Oligopeptide Vaccine in a Murine Model of Chlamydia trachomatis Genital Tract Infection: Serum Neutralizing IgG Antibodies Do Not Protect against Chlamydial Genital Tract Infection","volume":"13","author":"Su","year":"1995","journal-title":"Vaccine"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"311120","DOI":"10.3389\/fimmu.2017.01652","article-title":"Protective Effect of Vaccine Promoted Neutralizing Antibodies against the Intracellular Pathogen Chlamydia trachomatis","volume":"8","author":"Olsen","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1038\/s41467-024-45526-2","article-title":"Immune Signature of Chlamydia Vaccine CTH522\/CAF\u00ae01 Translates from Mouse-to-Human and Induces Durable Protection in Mice","volume":"15","author":"Olsen","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1080\/14760584.2018.1435279","article-title":"Future of Human Chlamydia Vaccine: Potential of Self-Adjuvanting Biodegradable Nanoparticles as Safe Vaccine Delivery Vehicles","volume":"17","author":"Sahu","year":"2018","journal-title":"Expert. Rev. Vaccines"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.clim.2012.12.005","article-title":"Adjuvanted HLA-Supertype Restricted Subdominant Peptides Induce New T-Cell Immunity during Untreated HIV-1-Infection","volume":"146","author":"Karlsson","year":"2013","journal-title":"Clin. Immunol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"7098","DOI":"10.1016\/j.vaccine.2014.10.036","article-title":"A Novel Liposomal Adjuvant System, CAF01, Promotes Long-Lived Mycobacterium Tuberculosis-Specific T-Cell Responses in Human","volume":"32","author":"Joosten","year":"2014","journal-title":"Vaccine"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1016\/S1473-3099(19)30279-8","article-title":"Safety and Immunogenicity of the Chlamydia Vaccine Candidate CTH522 Adjuvanted with CAF01 Liposomes or Aluminium Hydroxide: A First-in-Human, Randomised, Double-Blind, Placebo-Controlled, Phase 1 Trial","volume":"19","author":"Abraham","year":"2019","journal-title":"Lancet Infect. Dis."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.coi.2009.05.016","article-title":"Heterologous Prime-Boost Vaccination","volume":"21","author":"Lu","year":"2009","journal-title":"Curr. Opin. Immunol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1038\/mi.2016.28","article-title":"Vaccine-Induced Th17 Cells Are Established as Resident Memory Cells in the Lung and Promote Local IgA Responses","volume":"10","author":"Christensen","year":"2017","journal-title":"Mucosal Immunol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/cei.13285","article-title":"Mucosal Vaccines and Technology","volume":"196","author":"Bentley","year":"2019","journal-title":"Clin. Exp. Immunol."},{"key":"ref_103","first-page":"79","article-title":"The Value of Synthetic Peptides as Vaccines for Eliciting T-Cell Immunity","volume":"130","author":"Schwartz","year":"1986","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1093\/infdis\/jiz438","article-title":"A Recombinant Chlamydia trachomatis MOMP Vaccine Elicits Cross-Serogroup Protection in Mice against Vaginal Shedding and Infertility","volume":"221","author":"Tifrea","year":"2020","journal-title":"J. Infect. Dis."},{"key":"ref_105","unstructured":"World Health Organization (2024, April 08). International Clinical Trials Registry Platform (ICTRP). Available online: https:\/\/www.who.int\/clinical-trials-registry-platform."},{"key":"ref_106","first-page":"162","article-title":"A Multi-Component Prime-Boost Vaccination Regimen with a Consensus MOMP Antigen Enhances Chlamydia trachomatis Clearance","volume":"7","author":"McKay","year":"2016","journal-title":"Front. Immunol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"2543","DOI":"10.1016\/j.vaccine.2017.03.070","article-title":"Comparison of the Nine Polymorphic Membrane Proteins of Chlamydia trachomatis for Their Ability to Induce Protective Immune Responses in Mice against a C. Muridarum Challenge","volume":"35","author":"Pal","year":"2017","journal-title":"Vaccine"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"4672","DOI":"10.1016\/j.vaccine.2014.06.002","article-title":"Evaluation of a Multisubunit Recombinant Polymorphic Membrane Protein and Major Outer Membrane Protein T Cell Vaccine against Chlamydia Muridarum Genital Infection in Three Strains of Mice","volume":"32","author":"Yu","year":"2014","journal-title":"Vaccine"},{"key":"ref_109","first-page":"440","article-title":"Protective Effects of Fusion Protein of Hsp65-MOMP-T-Epitopes on C. Trachomatis Genital Tract Infection of Mice","volume":"33","author":"Yang","year":"2007","journal-title":"J. Jilin Univ. Med. Ed."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"9969","DOI":"10.1073\/pnas.1101756108","article-title":"Approach to Discover T- and B-Cell Antigens of Intracellular Pathogens Applied to the Design of Chlamydia trachomatis Vaccines","volume":"108","author":"Finco","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.4049\/jimmunol.173.5.3375","article-title":"A Novel Recombinant Multisubunit Vaccine against Chlamydia","volume":"173","author":"Eko","year":"2004","journal-title":"J. Immunol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"3883","DOI":"10.1016\/j.vaccine.2017.05.063","article-title":"Multistage Vaccines Containing Outer Membrane, Type III Secretion System and Inclusion Membrane Proteins Protects against a Chlamydia Genital Tract Infection and Pathology","volume":"35","author":"Armitage","year":"2017","journal-title":"Vaccine"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1128\/IAI.01377-07","article-title":"The Chlamydial Plasmid-Encoded Protein Pgp3 Is Secreted into the Cytosol of Chlamydia-Infected Cells","volume":"76","author":"Li","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"Slepenkin, A., Pal, S., Hoang-Phou, S., Abisoye-Ogunniyan, A., Rasley, A., D\u2019haeseleer, P., Coleman, M.A., and de la Maza, L.M. (2023). The Polymorphic Membrane Protein G Has a Neutral Effect and the Plasmid Glycoprotein 3 an Antagonistic Effect on the Ability of the Major Outer Membrane Protein to Elicit Protective Immune Responses against a Chlamydia Muridarum Respiratory Challenge. Vaccines, 11.","DOI":"10.3390\/vaccines11030504"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"6330","DOI":"10.1128\/IAI.72.11.6330-6340.2004","article-title":"The Infecting Dose of Chlamydia Muridarum Modulates the Innate Immune Response and Ascending Infection","volume":"72","author":"Maxion","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1128\/iai.58.6.2021-2023.1990","article-title":"Variation in Virulence among Oculogenital Serovars of Chlamydia trachomatis in Experimental Genital Tract Infection","volume":"58","author":"Ito","year":"1990","journal-title":"Infect. Immun."},{"key":"ref_117","first-page":"383","article-title":"Comparison of Multiple Genital Tract Infections with Chlamydia trachomatis in Different Strains of Female Mice","volume":"38","author":"Lyons","year":"2005","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1128\/iai.65.8.3065-3073.1997","article-title":"Mouse Strain-Dependent Variation in the Course and Outcome of Chlamydial Genital Tract Infection Is Associated with Differences in Host Response","volume":"65","author":"Darville","year":"1997","journal-title":"Infect. Immun."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"4212","DOI":"10.4049\/jimmunol.164.8.4212","article-title":"Suppression of Endogenous IL-10 Gene Expression in Dendritic Cells Enhances Antigen Presentation for Specific Th1 Induction: Potential for Cellular Vaccine Development","volume":"164","author":"Igietseme","year":"2000","journal-title":"J. Immunol."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/6\/1196\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:58:20Z","timestamp":1760108300000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/6\/1196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,13]]},"references-count":119,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["microorganisms12061196"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12061196","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,13]]}}}