{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T17:55:48Z","timestamp":1779040548172,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T00:00:00Z","timestamp":1718236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Oral Health Research and Innovation Fund","award":["20221111"],"award-info":[{"award-number":["20221111"]}]},{"name":"Teaching Development Grant, The University of Hong Kong","award":["20221111"],"award-info":[{"award-number":["20221111"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Removable denture wearers are at an increased risk of developing periodontal diseases due to biofilm deposition and microbial colonization on the denture surface. This study aimed to characterize and compare the metagenomic composition of saliva in denture wearers with different periodontal statuses. Twenty-four community-dwelling elders were recruited and grouped into denture wearers with active periodontitis (APD), non-denture wearers with active periodontitis (APXD), denture wearers with stable periodontal health conditions (SPCD), and non-denture wearers with stable periodontal health conditions (SPCXD). Saliva samples were collected and underwent Type IIB restriction-site-associated DNA for microbiome (2bRAD-M) metagenomic sequencing to characterize the species-resolved microbial composition. Alpha diversity analysis based on the Shannon index revealed no significant difference between groups. Beta diversity analysis using the Jaccard distance matrix was nearly significantly different between denture-wearing and non-denture-wearing groups (p = 0.075). Some respiratory pathogens, including Streptococcus agalactiae and Streptococcus pneumoniae, were detected as the top 30 species in saliva samples. Additionally, LEfSe analysis revealed a substantial presence of pathogenic bacteria in denture groups. In the cohort of saliva samples collected from community-dwelling elders, a remarkable abundance of certain opportunistic pathogens was detected in the microbial community.<\/jats:p>","DOI":"10.3390\/microorganisms12061197","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T03:52:53Z","timestamp":1718337173000},"page":"1197","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Metagenomic Characterization and Comparative Analysis of Removable Denture-Wearing and Non-Denture-Wearing Individuals in Healthy and Diseased Periodontal Conditions"],"prefix":"10.3390","volume":"12","author":[{"given":"Ho-Hin","family":"Wong","sequence":"first","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Ho","family":"Hung","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Yip","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3506-9388","authenticated-orcid":false,"given":"Tong-Wah","family":"Lim","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1099\/mic.0.26082-0","article-title":"Are dental diseases examples of ecological catastrophes?","volume":"149","author":"Marsh","year":"2003","journal-title":"Microbiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1046\/j.1365-2842.2000.00512.x","article-title":"Oral health status of patients 5\u20136 years after placement of cobalt\u2013chromium removable partial dentures","volume":"27","author":"Yeung","year":"2000","journal-title":"J. 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