{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T13:06:27Z","timestamp":1765976787079,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T00:00:00Z","timestamp":1720915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANID scholarship","award":["21201993","1230547"],"award-info":[{"award-number":["21201993","1230547"]}]},{"name":"the project Fondecyt","award":["21201993","1230547"],"award-info":[{"award-number":["21201993","1230547"]}]},{"name":"the Conicyt FB 0001 project","award":["21201993","1230547"],"award-info":[{"award-number":["21201993","1230547"]}]},{"name":"the Center for Biotechnology and Bioengineering (CeBiB)","award":["21201993","1230547"],"award-info":[{"award-number":["21201993","1230547"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>This work analyzes the production of total lipids and the content of CoQ9 and CoQ10 in the biomass of Thraustochytrium sp. RT2316-16 grown in media containing a single amino acid at a concentration of 1 g L\u22121 as the sole nitrogen source; glucose (5 g L\u22121) was used as the carbon source. Biomass concentration and the content of total lipids and CoQ were determined as a function of the incubation time; ten amino acids were evaluated. The final concentration of the total biomass was found to be between 2.2 \u00b1 0.1 (aspartate) and 3.9 \u00b1 0.1 g L\u22121 (glutamate). The biomass grown in media containing glutamate, serine or phenylalanine reached a content of total lipids higher than 20% of the cell dry weight (DW) after 72, 60 and 72 h of incubation, respectively. The highest contents of CoQ9 (39.0 \u00b1 0.7 \u00b5g g\u22121 DW) and CoQ10 (167.4 \u00b1 3.4 mg g\u22121 DW) in the biomass of the thraustochytrid were obtained when glutamate and cysteine were used as the nitrogen source, respectively. Fatty acid oxidation, which decreased the total lipid content during the first 12 h of incubation, and the oxidation of hydrogen sulfide when cysteine was the nitrogen source, might be related to the content of CoQ10 in the biomass of the thraustochytrid.<\/jats:p>","DOI":"10.3390\/microorganisms12071428","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T08:35:17Z","timestamp":1721032517000},"page":"1428","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Single Amino Acids as Sole Nitrogen Source for the Production of Lipids and Coenzyme Q by Thraustochytrium sp. RT2316-16"],"prefix":"10.3390","volume":"12","author":[{"given":"Liset","family":"Flores","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Center of Food Biotechnology and Bioseparations, BIOREN, and Centre of Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Temuco 4780000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4646-038X","authenticated-orcid":false,"given":"Carolina","family":"Shene","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Center of Food Biotechnology and Bioseparations, BIOREN, and Centre of Biotechnology and Bioengineering (CeBiB), Universidad de La Frontera, Temuco 4780000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3296","DOI":"10.1093\/hmg\/ddaa214","article-title":"Coenzyme Q10 modulates sulfide metabolism and links the mitochondrial respiratory chain to pathways associated to one carbon metabolism","volume":"29","author":"Mascaraque","year":"2020","journal-title":"Hum. 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