{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T14:01:17Z","timestamp":1784901677077,"version":"3.55.0"},"reference-count":38,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Recent investigations have highlighted, both experimentally and clinically, that probiotic strains equipped with arabinofuranosidase, in particular abfA and abfB, favor regular intestinal motility, thus counteracting constipation. By analyzing the gene expression and the proliferative response in the presence of arabinan of the probiotic B. longum W11, a strain previously validated as an anti-constipation probiotic, we have speculated that its response mechanism to arabinan can effectively explain its clinical action. Our approach could be used in the future to select probiotics endowed with arabinofuranosidase-related anti-constipation effects.<\/jats:p>","DOI":"10.3390\/microorganisms12081626","type":"journal-article","created":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T07:59:07Z","timestamp":1723190347000},"page":"1626","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Anti-Constipation Effect of Bifidobacterium Longum W11 Is Likely Due to a Key Genetic Factor Governing Arabinan Utilization"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6654-8675","authenticated-orcid":false,"given":"Francesco","family":"Di Pierro","sequence":"first","affiliation":[{"name":"Microbiota International Clinical Society, 10123 Torino, Italy"},{"name":"Scientific & Research Department, Velleja Research, 20125 Milano, Italy"},{"name":"Department of Medicine and Technological Innovation, University of Insubria, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Zerbinati","sequence":"additional","affiliation":[{"name":"Department of Medicine and Technological Innovation, University of Insubria, 21100 Varese, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimiliano","family":"Cazzaniga","sequence":"additional","affiliation":[{"name":"Scientific & Research Department, Velleja Research, 20125 Milano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3922-9115","authenticated-orcid":false,"given":"Alexander","family":"Bertuccioli","sequence":"additional","affiliation":[{"name":"Microbiota International Clinical Society, 10123 Torino, Italy"},{"name":"Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61122 Urbino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara Maria","family":"Palazzi","sequence":"additional","affiliation":[{"name":"Microbiota International Clinical Society, 10123 Torino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2326-2328","authenticated-orcid":false,"given":"Ilaria","family":"Cavecchia","sequence":"additional","affiliation":[{"name":"Microbiota International Clinical Society, 10123 Torino, Italy"},{"name":"Microbiomic Department, Koelliker Hospital, 10134 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2115-1701","authenticated-orcid":false,"given":"Mariarosaria","family":"Matera","sequence":"additional","affiliation":[{"name":"Microbiota International Clinical Society, 10123 Torino, Italy"},{"name":"Department of Paediatric Emergencies, Misericordia Hospital, 58100 Grosseto, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edoardo","family":"Labrini","sequence":"additional","affiliation":[{"name":"AAT\u2014Advanced Analytical Technologies, Fiorenzuola d\u2019Arda, 29017 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Sagheddu","sequence":"additional","affiliation":[{"name":"AAT\u2014Advanced Analytical Technologies, Fiorenzuola d\u2019Arda, 29017 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5670-1306","authenticated-orcid":false,"given":"Sara","family":"Soldi","sequence":"additional","affiliation":[{"name":"AAT\u2014Advanced Analytical Technologies, Fiorenzuola d\u2019Arda, 29017 Piacenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,9]]},"reference":[{"key":"ref_1","first-page":"e52013","article-title":"Effectiveness of Probiotics in Patients with Constipation: A Systematic Review and Meta-Analysis","volume":"16","author":"Jaramillo","year":"2024","journal-title":"Cureus"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1016\/j.clnu.2022.10.015","article-title":"Probiotics and synbiotics in chronic constipation in adults: A systematic review and meta-analysis of randomized controlled trials","volume":"41","author":"Helander","year":"2022","journal-title":"Clin. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Dong, M., Wu, Y., Zhang, M., Chen, P., Zhang, Z., and Wang, S. (2023). Effect of probiotics intake on constipation in children: An umbrella review. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1218909"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1016\/j.clnu.2020.01.005","article-title":"Meta-analysis of randomized controlled trials of the effects of probiotics on functional constipation in adults","volume":"39","author":"Zhang","year":"2020","journal-title":"Clin. Nutr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"484","DOI":"10.3945\/an.116.014407","article-title":"Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation","volume":"8","author":"Dimidi","year":"2017","journal-title":"Adv. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/s41575-019-0222-y","article-title":"Management of functional constipation in children and adults","volume":"17","author":"Vriesman","year":"2020","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1016\/j.chom.2023.10.011","article-title":"A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation","volume":"31","author":"Zhang","year":"2023","journal-title":"Cell Host Microbe"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S153","DOI":"10.1097\/MCG.0000000000000683","article-title":"The Possible Innovative Use of Bifidobacterium longum W11 in Association with Rifaximin: A New Horizon for Combined Approach?","volume":"50","author":"Graziano","year":"2015","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_9","first-page":"259","article-title":"Effects of rifaximin-resistant Bifidobacterium longum W11 in subjects with symptomatic uncomplicated diverticular disease treated with rifaximin","volume":"65","author":"Bertuccioli","year":"2019","journal-title":"Minerva Gastroenterol. Dietol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.clnesp.2020.12.025","article-title":"Bifidobacterium longum W11: Uniqueness and individual or combined clinical use in association with rifaximin","volume":"42","author":"Pane","year":"2021","journal-title":"Clin. Nutr. ESPEN"},{"key":"ref_11","first-page":"349","article-title":"Effect of a symbiotic preparation on the clinical manifestations of irritable bowel syndrome, constipation-variant. Results of an open, uncontrolled multicenter study","volume":"52","author":"Colecchia","year":"2006","journal-title":"Minerva Gastroenterol. Dietol."},{"key":"ref_12","first-page":"111","article-title":"Effects of symbiotic preparations on constipated irritable bowel syndrome symptoms","volume":"78","author":"Dughera","year":"2007","journal-title":"Acta Biomed."},{"key":"ref_13","first-page":"157","article-title":"Diet and chronic constipation. Benefits of oral supplementation with symbiotic zir fos (Bifidobacterium longum W11 + FOS Actilight)","volume":"77","author":"Amenta","year":"2006","journal-title":"Acta Biomed."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Savard, P., and Roy, D. (2009). Determination of Differentially Expressed Genes Involved in Arabinoxylan Degradation by Bifidobacterium longum NCC2705 Using Real-Time RT-PCR. Probiotics Antimicrob. Proteins, 1.","DOI":"10.1007\/s12602-009-9015-x"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e02582-18","DOI":"10.1128\/AEM.02582-18","article-title":"Two Novel \u03b1-l-Arabinofuranosidases from Bifidobacterium longum subsp. longum Belonging to Glycoside Hydrolase Family 43 Cooperatively Degrade Arabinan","volume":"85","author":"Komeno","year":"2019","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.carbpol.2015.05.022","article-title":"In vitro digestion and fermentation properties of linear sugar-beet arabinan and its oligosaccharides","volume":"131","author":"Moon","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_17","unstructured":"(2010). Milk Products-Enumeration of Presumptive Bifidobacteria-Colony Count Technique at 37 \u00b0C (Standard No. ISO 29981:2010|IDF). Available online: https:\/\/www.iso.org\/standard\/45765.html."},{"key":"ref_18","first-page":"251","article-title":"Roles of short-chain fatty acids receptors, GPR41 and GPR43 on colonic functions","volume":"59","author":"Tazoe","year":"2008","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1053\/j.gastro.2010.01.053","article-title":"Short-chain fatty acids regulate the enteric neurons and control gastrointestinal motility in rats","volume":"138","author":"Soret","year":"2010","journal-title":"Gastroenterology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1016\/j.cell.2020.08.007","article-title":"Longitudinal multi-omics reveals subset-specific mechanisms underlying irritable bowel syndrome","volume":"182","author":"Mars","year":"2020","journal-title":"Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/j.chom.2022.03.036","article-title":"Global, distinctive, and personal changes in molecular and microbial profiles by specific fibers in humans","volume":"30","author":"Lancaster","year":"2022","journal-title":"Cell Host Microbe"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1177\/030006058301100211","article-title":"Treatment of constipation with chenodeoxycholic acid","volume":"11","author":"Bazzoli","year":"1983","journal-title":"J. Int. Med. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1053\/j.gastro.2010.07.052","article-title":"Chenodeoxycholate in females with irritable bowel syndrome-constipation: A pharmacodynamic and pharmacogenetic analysis","volume":"139","author":"Rao","year":"2010","journal-title":"Gastroenterology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1053\/j.gastro.2012.09.055","article-title":"The receptor TGR5 mediates the prokinetic actions of intestinal bile acids and is required for normal defecation in mice","volume":"144","author":"Alemi","year":"2013","journal-title":"Gastroenterology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1038\/s41564-020-0721-1","article-title":"Moving from probiotics to precision probiotics","volume":"5","author":"Veiga","year":"2020","journal-title":"Nat. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Pascale, N., Gu, F., Larsen, N., Jespersen, L., and Respondek, F. (2022). The Potential of Pectins to Modulate the Human Gut Microbiota Evaluated by In Vitro Fermentation: A Systematic Review. Nutrients, 14.","DOI":"10.3390\/nu14173629"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Zannini, E., Bravo N\u00fa\u00f1ez, \u00c1., Sahin, A.W., and Arendt, E.K. (2022). Arabinoxylans as Functional Food Ingredients: A Review. Foods, 11.","DOI":"10.3390\/foods11071026"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1099\/ijsem.0.004107","article-title":"A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae","volume":"70","author":"Zheng","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Khatri, I., Sharma, G., and Subramanian, S. (2019). Composite genome sequence of Bacillus clausii, a probiotic commercially available as Enterogermina\u00ae, and insights into its probiotic properties. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1680-7"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"92","DOI":"10.3390\/microorganisms2020092","article-title":"The Science behind the Probiotic Strain Bifidobacterium animalis subsp. lactis BB-12(\u00ae)","volume":"2","author":"Jungersen","year":"2014","journal-title":"Microorganisms"},{"key":"ref_31","first-page":"e1","article-title":"Approach to probiotics in pediatrics: The role of Lactobacillus rhamnosus GG","volume":"120","author":"Burgos","year":"2022","journal-title":"Arch. Argent. Pediatr."},{"key":"ref_32","first-page":"69","article-title":"Systematic review of randomised controlled trials: Probiotics for functional constipation","volume":"16","author":"Chmielewska","year":"2010","journal-title":"World J. Gastroenterol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"S10","DOI":"10.1097\/MCG.0000000000001054","article-title":"Effectiveness of Probiotics in Children with Functional Abdominal Pain Disorders and Functional Constipation: A Systematic Review","volume":"52","author":"Wegh","year":"2017","journal-title":"J. Clin. Gastroenterol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s13312-023-2907-0","article-title":"Efficacy of Bacillus clausii in Pediatric Functional Constipation: A Pilot of a Randomized, Double-Blind, Placebo-Controlled Trial","volume":"60","author":"Lojanatorn","year":"2023","journal-title":"Indian Pediatr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s40801-023-00402-1","article-title":"A Prospective Real-World Study of Bacillus clausii Evaluating Use, Treatment Habits and Patient Satisfaction in Italian Community Pharmacies: The PEGASO Study","volume":"11","author":"Giua","year":"2024","journal-title":"Drugs\u2013Real World Outcomes"},{"key":"ref_36","first-page":"305","article-title":"Fermented cereal with specific bifidobacteria normalizes bowel movements in elderly nursing home residents. A randomized, controlled trial","volume":"11","author":"Pitkala","year":"2007","journal-title":"J. Nutr. Health Aging"},{"key":"ref_37","first-page":"39","article-title":"Effect of fermented milk containing Bifidobacterium lactis BB-12\u00ae on stool frequency, defecation, fecal microbiota and safety of excessive ingestion in healthy female students","volume":"8","author":"Uchida","year":"2005","journal-title":"J. Nutr. Food"},{"key":"ref_38","first-page":"71","article-title":"Effect of yogurt containing Bifidobacterium lactis BB-12 on improvement of defecation and fecal microflora of healthy female adults","volume":"53","author":"Nishida","year":"2004","journal-title":"Milk Sci."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/8\/1626\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:32:42Z","timestamp":1760110362000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/8\/1626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,9]]},"references-count":38,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["microorganisms12081626"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12081626","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,9]]}}}