{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:12:23Z","timestamp":1788394343751,"version":"build-2803163510"},"reference-count":70,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T00:00:00Z","timestamp":1723420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DGAPA, UNAM","award":["IN227023"],"award-info":[{"award-number":["IN227023"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The traditional Mexican fermented beverage pulque has been considered a healthy product for treating gastrointestinal disorders. Lactic acid bacteria (LAB) have been identified as one of the most abundant microbial groups during pulque fermentation. As traditional pulque is consumed directly from the fermentation vessel, the naturally associated LABs are ingested, reaching the consumer\u2019s small intestine alive, suggesting their potential probiotic capability. In this contribution, we assayed the probiotic potential of the strain of Lactiplantibacillus plantarum LB1_P46 isolated from pulque produced in Huitzilac, Morelos State, Mexico. The characterization included resistance to acid pH (3.5) and exposure to bile salts at 37 \u00b0C; the assay of the hemolytic activity and antibiotic resistance profiling; the functional traits of cholesterol reduction and \u03b2-galactosidase activity; and several cell surface properties, indicating that this LAB possesses probiotic properties comparable to other LAB. Additionally, this L. plantarum showed significance in in vitro antimicrobial activity against several Gram-negative and Gram-positive bacteria and in vivo preventive anti-infective capability against Salmonella in a BALB\/c mouse model. Several functional traits and probiotic activities assayed were correlated with the corresponding enzymes encoded in the complete genome of the strain. The genome mining for bacteriocins led to the identification of several bacteriocins and a ribosomally synthesized and post-translationally modified peptide encoding for the plantaricin EF. Results indicated that L. plantarum LB1_P46 is a promising probiotic LAB for preparing functional non-dairy and dairy beverages.<\/jats:p>","DOI":"10.3390\/microorganisms12081652","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T10:34:59Z","timestamp":1723458899000},"page":"1652","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Analysis of the Probiotic Potential of Lactiplantibacillus plantarum LB1_P46 Isolated from the Mexican Fermented Pulque Beverage: A Functional and Genomic Analysis"],"prefix":"10.3390","volume":"12","author":[{"given":"Martha","family":"Giles-G\u00f3mez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda, Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximena","family":"Morales Huerta","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Pastelin-Palacios","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0530-492X","authenticated-orcid":false,"given":"Constantino","family":"L\u00f3pez-Mac\u00edas","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Inmunoqu\u00edmica, Hospital de Especialidades del Centro M\u00e9dico Nacional \u201cSiglo XXI\u201d, Instituto Mexicano del Seguro Social (IMSS), Cuauht\u00e9moc, Ciudad de M\u00e9xico 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayrene Sarai","family":"Flores Montesinos","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Celular y Biocat\u00e1lisis, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca 62210, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8932-4326","authenticated-orcid":false,"given":"Fernando","family":"Astudillo-Melgar","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Celular y Biocat\u00e1lisis, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca 62210, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5895-3694","authenticated-orcid":false,"given":"Adelfo","family":"Escalante","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Celular y Biocat\u00e1lisis, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Cuernavaca 62210, Morelos, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,12]]},"reference":[{"key":"ref_1","unstructured":"Moreno-Terrazas, R., Escalante, A., Verdugo-Valdez, A.G., De la Rosa, M., Ortiz Basurto, R.I., Orantes-Garc\u00eda, C., Palafox-Gonz\u00e1lez, L., Castro D\u00edaz, A.S., and Lappe-Oliveras, P. 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