{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:55:58Z","timestamp":1791485758893,"version":"4.3.4"},"reference-count":28,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T00:00:00Z","timestamp":1723507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Innovation and Universities, Spain","award":["PID2019-108722RB-C33"],"award-info":[{"award-number":["PID2019-108722RB-C33"]}]},{"name":"Ministry of Science, Innovation and Universities, Spain","award":["2020PMF-PIPF-49"],"award-info":[{"award-number":["2020PMF-PIPF-49"]}]},{"name":"Ministry of Science, Innovation and Universities, Spain","award":["872394-vWISE-H2020-MSCA-RISE-2019"],"award-info":[{"award-number":["872394-vWISE-H2020-MSCA-RISE-2019"]}]},{"name":"Mart\u00ed Franqu\u00e8s Fellowship from the Universitat Rovira i Virgili (URV)","award":["PID2019-108722RB-C33"],"award-info":[{"award-number":["PID2019-108722RB-C33"]}]},{"name":"Mart\u00ed Franqu\u00e8s Fellowship from the Universitat Rovira i Virgili (URV)","award":["2020PMF-PIPF-49"],"award-info":[{"award-number":["2020PMF-PIPF-49"]}]},{"name":"Mart\u00ed Franqu\u00e8s Fellowship from the Universitat Rovira i Virgili (URV)","award":["872394-vWISE-H2020-MSCA-RISE-2019"],"award-info":[{"award-number":["872394-vWISE-H2020-MSCA-RISE-2019"]}]},{"name":"vWISE Project (UE)","award":["PID2019-108722RB-C33"],"award-info":[{"award-number":["PID2019-108722RB-C33"]}]},{"name":"vWISE Project (UE)","award":["2020PMF-PIPF-49"],"award-info":[{"award-number":["2020PMF-PIPF-49"]}]},{"name":"vWISE Project (UE)","award":["872394-vWISE-H2020-MSCA-RISE-2019"],"award-info":[{"award-number":["872394-vWISE-H2020-MSCA-RISE-2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Controlling the microorganisms involved in alcoholic fermentation during wine production can be achieved by adding a small quantity of spontaneously fermenting must to freshly crushed grapes, a technique known as pied de cuve (PdC). This method not only serves as an inoculation starter but also enhances the microbial footprint unique to each wine region. Recent studies have confirmed that wines inoculated with PdC exhibit efficient fermentation kinetics comparable to those inoculated with commercial strains of Saccharomyces cerevisiae. However, further research is required to draw robust conclusions about the chemical and sensory impacts of PdC-inoculated wines. In this study, we examined the chemical and sensory effects of the PdC technique across three different harvests: Muscat of Alexandria (Spain, harvests 2022 and 2023) and Sauvignon Blanc (Chile, harvest 2023). Each PdC was prepared using various stressors (sulfur dioxide, ethanol, and temperature). Our findings revealed that wines produced with PdC exhibited similar fermentation kinetics and sensory profiles to those inoculated with commercial strains. Notably, PdC fermentations resulted in lower concentrations of acetic acid compared to both the commercial strain and spontaneous fermentations. The sensory analysis indicated that PdC wines significantly differed from those made with commercial strains, with PdC wines displaying more pronounced tropical notes. These results suggest that the PdC technique, particularly when using specific stressors, can maintain desirable fermentation characteristics while enhancing certain sensory attributes, offering a viable alternative to traditional inoculation methods.<\/jats:p>","DOI":"10.3390\/microorganisms12081655","type":"journal-article","created":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T06:02:37Z","timestamp":1723528957000},"page":"1655","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Impact of the Inoculation of Different Pied de Cuve on the Chemical and Organoleptic Profiles of Wines"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4102-4944","authenticated-orcid":false,"given":"Katherine","family":"Bedoya","sequence":"first","affiliation":[{"name":"Biotecnolog\u00eda Enol\u00f3gica, Department Bioqu\u00edmica i Biotecnologia, Facultat d\u2018Enologia, Universitat Rovira i Virgili, C\/Marcel\u00b7l\u00ed Domingo 1, 43007 Tarragona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0763-1679","authenticated-orcid":false,"given":"Albert","family":"Mas","sequence":"additional","affiliation":[{"name":"Biotecnolog\u00eda Enol\u00f3gica, Department Bioqu\u00edmica i Biotecnologia, Facultat d\u2018Enologia, Universitat Rovira i Virgili, C\/Marcel\u00b7l\u00ed Domingo 1, 43007 Tarragona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9718-3429","authenticated-orcid":false,"given":"Nicolas","family":"Roz\u00e8s","sequence":"additional","affiliation":[{"name":"Biotecnologia Microbiana dels Aliments, Department Bioqu\u00edmica i Biotecnologia, Facultat d\u2018Enologia, Universitat Rovira i Virgili, C\/Marcel\u00b7l\u00ed Domingo 1, 43007 Tarragona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Jara","sequence":"additional","affiliation":[{"name":"Departmento de Agroindustria y Enolog\u00eda, Facultad de Ciencias Agron\u00f3micas, Universidad de Chile Santa Rosa, Santiago 11315, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4576-0244","authenticated-orcid":false,"given":"Mar\u00eda del Carmen","family":"Portillo","sequence":"additional","affiliation":[{"name":"Biotecnolog\u00eda Enol\u00f3gica, Department Bioqu\u00edmica i Biotecnologia, Facultat d\u2018Enologia, Universitat Rovira i Virgili, C\/Marcel\u00b7l\u00ed Domingo 1, 43007 Tarragona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ciani, M., Capece, A., Comitini, F., Canonico, L., Siesto, G., and Romano, P. (2016). Yeast interactions in inoculated wine fermentation. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00555"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Pinu, F.R., Stuart, L., Topal, T., Albright, A., Martin, D., and Grose, C. (2023). The effect of yeast inoculation methods on the metabolite composition of Sauvignon Blanc wines. Fermentation, 9.","DOI":"10.3390\/fermentation9080759"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Molinet, J., and Cubillos, F.A. (2020). Wild yeast for the future: Exploring the use of wild strains for wine and beer fermentation. Front. Genet., 11.","DOI":"10.3389\/fgene.2020.589350"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.lwt.2012.06.011","article-title":"Improving alcoholic fermentation by activation of Saccharomyces species during the rehydration stage","volume":"50","author":"Briones","year":"2013","journal-title":"LWT"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"363","DOI":"10.5344\/ajev.2004.55.4.363","article-title":"Effect of Yeast inoculation rate, acclimatization, and nutrient addition on icewine fermentation","volume":"55","author":"Kontkanen","year":"2004","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.ijfoodmicro.2008.05.016","article-title":"Vitality enhancement of the rehydrated active dry wine yeast","volume":"126","author":"Novo","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Belda, I., Ruiz, J., Esteban-Fern\u00e1ndez, A., Navascu\u00e9s, E., Marquina, D., Santos, A., and Moreno Arribas, M.V. (2017). Microbial contribution to wine aroma and its intended use for wine quality improvement. Molecules, 22.","DOI":"10.3390\/molecules22020189"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mas, A., and Portillo, M.C. (2022). Strategies for microbiological control of the alcoholic fermentation in wines by exploiting the microbial terroir complexity: A mini-review. Int. J. Food Microbiol., 367.","DOI":"10.1016\/j.ijfoodmicro.2022.109592"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.cofs.2020.04.004","article-title":"Indigenous yeasts: Emerging trends and challenges in winemaking","volume":"32","author":"Lappa","year":"2020","journal-title":"Curr. Opin. Food Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/yea.3219","article-title":"Yeasts found in vineyards and wineries","volume":"34","author":"Varela","year":"2017","journal-title":"Yeast"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Morata, A., Loira, I., and Gonz\u00e1lez, C. (2022). An overview on Saccharomyces cerevisiae indigenous strains selection methods. Grapes and Wine, IntechOpen.","DOI":"10.5772\/intechopen.95210"},{"key":"ref_12","first-page":"435","article-title":"The \u201cpied de cuve\u201d as an alternative way to manage indigenous fermentation: Impact on the fermentative process and Saccharomyces cerevisiae diversity","volume":"54","author":"Vinsonneau","year":"2020","journal-title":"OENO One"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"113648","DOI":"10.1016\/j.foodres.2023.113648","article-title":"Influence of spontaneous, \u201cpied de cuve\u201d and commercial dry yeast fermentation strategies on wine molecular composition and sensory properties","volume":"174","author":"Mallard","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"960","DOI":"10.4014\/jmb.1111.11013","article-title":"Evaluation of yeast diversity during wine fermentations with direct inoculation and pied de cuve method at an industrial scale","volume":"22","author":"Li","year":"2012","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Bedoya, K., Buetas, L., Roz\u00e8s, N., Mas, A., and Portillo, M.C. (2024). Influence of different stress factors during the elaboration of grape must\u2019s pied de cuve on the dynamics of yeast populations during alcoholic fermentation. Food Microbiol., 123.","DOI":"10.1016\/j.fm.2024.104571"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ruiz-de-Villa, C., Gombau, J., Poblet, M., Bordons, A., Canals, J.M., Zamora, F., Reguant, C., and Roz\u00e8s, N. (2023). Torulaspora delbrueckii improves organoleptic properties and promotes malolactic fermentation in carbonic maceration wines. Fermentation, 9.","DOI":"10.3390\/fermentation9121021"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Rib\u00e9reau-Gayon, P., Dubourdieu, D., Don\u00e8che, B., and Lonvaud, A. (2006). Handbook of Enology: The Microbiology of Wine and Vinifications, John Wiley & Sons Ltd.. [2nd ed.].","DOI":"10.1002\/0470010363"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/ajgw.12166","article-title":"Use of fortified pied de cuve as an innovative method to start spontaneous alcoholic fermentation for red winemaking","volume":"22","author":"Moschetti","year":"2016","journal-title":"Aust. J. Grape Wine Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/j.fm.2011.03.008","article-title":"Quantifying the individual effects of ethanol and temperature on the fitness advantage of Saccharomyces cerevisiae","volume":"28","author":"Barrio","year":"2011","journal-title":"Food Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5344\/ajev.1972.23.1.1","article-title":"Glycerol in wine: Determination and some factors affecting","volume":"23","author":"Ough","year":"1972","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"36","DOI":"10.5344\/ajev.1982.33.1.36","article-title":"Glycerol production of various strains of Saccharomyces","volume":"33","author":"Radler","year":"1982","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_22","first-page":"22","article-title":"Glycerol in South African table wines: An assessment of its relationship to wine quality","volume":"23","author":"Nieuwoudtl","year":"2002","journal-title":"SAJEV"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1755-0238.2007.tb00070.x","article-title":"The effects of ethanol and glycerol on the body and other sensory characteristics of Riesling wines","volume":"13","author":"Gawel","year":"2007","journal-title":"Aust. J. Grape Wine Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.foodqual.2008.03.005","article-title":"The influence of interactions between major white wine components on the aroma, flavour and texture of model white wine","volume":"19","author":"Jones","year":"2008","journal-title":"Food Qual. Prefer."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"371","DOI":"10.5344\/ajev.2004.55.4.371","article-title":"Impact of yeast strain on the production of acetic acid, glycerol, and the sensory attributes of Icewine","volume":"55","author":"Erasmus","year":"2004","journal-title":"Am. J. Enol. Vitic."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/1567-1364.12111","article-title":"Not your ordinary yeast: Non-Saccharomyces yeasts in wine production uncovered","volume":"14","author":"Jolly","year":"2014","journal-title":"FEMS Yeast Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.fm.2012.09.014","article-title":"Dynamic study of yeast and Saccharomyces cerevisiae strains during the spontaneous fermentations of Muscat blanc in Jingyang","volume":"33","author":"Wang","year":"2013","journal-title":"Food Microbiol."},{"key":"ref_28","unstructured":"Jackson, R.S. (2008). Wine Science: Principles and Applications, Academic Press. [3rd ed.]."}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/8\/1655\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:35:41Z","timestamp":1760110541000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/8\/1655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,13]]},"references-count":28,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["microorganisms12081655"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12081655","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,13]]}}}