{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:13:05Z","timestamp":1788297185116,"version":"build-2803163510"},"reference-count":65,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CEVA Hellas","award":["7652 (University of Thessaly)"],"award-info":[{"award-number":["7652 (University of Thessaly)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The objective of this study was to apply and preliminarily evaluate a High-Resolution Melting (HRM) analysis technique coupled with qPCR, that allows the simultaneous detection of 10 different ruminant abortogenic pathogens, for investigating abortions in sheep and goats throughout Greece. A total of 264 ovine and caprine vaginal swabs were obtained the week following the abortion from aborted females and analyzed using a commercially available kit (ID Gene\u2122 Ruminant Abortion Multiplex HRM, Innovative Diagnostics). Results indicated a high prevalence of Coxiella burnetii and Chlamydophila spp., which were detected in 48.9% and 42.4% of the vaginal swabs, respectively. Results for these most commonly detected pathogens were compared with those of a well-established commercial qPCR kit, with near-perfect agreement. Toxoplasma gondii, Salmonella spp., Brucella spp., Anaplasma phagocytophilum, Campylobacter fetus, and Neospora caninum were also identified, the two latter reported for the first time in the country in small ruminants. Mixed infections occurred in 35.6% of the animals examined. This technique allows for the simultaneous detection of many abortogenic pathogens in an accurate and cost-effective assay. Detection of uncommon or not previously reported pathogens in various cases indicates that their role in ovine and caprine abortions may be underestimated.<\/jats:p>","DOI":"10.3390\/microorganisms12081675","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T09:20:51Z","timestamp":1723627251000},"page":"1675","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular Investigation of Small Ruminant Abortions Using a 10-Plex HRM-qPCR Technique: A Novel Approach in Routine Diagnostics"],"prefix":"10.3390","volume":"12","author":[{"given":"Ioannis","family":"Gouvias","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0648-6004","authenticated-orcid":false,"given":"Marios","family":"Lysitsas","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4491-4387","authenticated-orcid":false,"given":"Apostolos","family":"Batsidis","sequence":"additional","affiliation":[{"name":"Department of Mathematics, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0756-3557","authenticated-orcid":false,"given":"Sonia","family":"Malefaki","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering and Aeronautics, University of Patras, 26500 Rion-Patras, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitra","family":"Bitchava","sequence":"additional","affiliation":[{"name":"Vet in Progress Plus, Veterinary Laboratories, 15343 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Tsara","sequence":"additional","affiliation":[{"name":"Vet in Progress Plus, Veterinary Laboratories, 15343 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilija","family":"Nickovic","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece"},{"name":"Faculty of Veterinary Medicine, University of Belgrade, Bul. Oslobodjenja 18, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3018-1426","authenticated-orcid":false,"given":"Ilias","family":"Bouzalas","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA (ELGO-DIMITRA), Campus Thermi, 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8956-2269","authenticated-orcid":false,"given":"Eleni","family":"Malissiova","sequence":"additional","affiliation":[{"name":"Food of Animal Origin Laboratory, Animal Science Department, University of Thessaly, 41500 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6658-522X","authenticated-orcid":false,"given":"Rapha\u00ebl","family":"Guatteo","sequence":"additional","affiliation":[{"name":"BIOEPAR, INRAE Oniris, 44300 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3869-5026","authenticated-orcid":false,"given":"George","family":"Valiakos","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.tvjl.2014.03.015","article-title":"Laboratory Diagnosis of Ruminant Abortion in Europe","volume":"200","author":"Borel","year":"2014","journal-title":"Vet. 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