{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T21:16:56Z","timestamp":1770585416127,"version":"3.49.0"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,17]],"date-time":"2024-08-17T00:00:00Z","timestamp":1723852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>(1) Background: Could compounds such as monoterpenes and sesquiterpenes present in essential plant oils inhibit bacterial growth as an alternative to help mitigate bacterial resistance? The purpose of this study is evaluating the in vitro antibacterial effect of Lippia organoides EO (LEO) and Thymus vulgaris EO (TEO), individually and in combination with ampicillin, against extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains; (2) Methods: Experimental in vitro design with post-test. The EOs were obtained by hydrodistillation and were analyzed by GC. ESBL-producing E. coli strains used were selected from urine cultures and the blaCTX-M and blaTEM resistance genes were identified by end point PCR. The disk diffusion method was used for the susceptibility tests. The MICs and MBCs were determined by microdilution test. Finally, the interaction effect was observed by checkerboard assay; (3) Results: A 39.9% decrease in the growth of the strain thymol in TEO and 70.4% in carvacrol in LEO was shown, observing inhibition halos of 32 mm for both EOs. MICs of 632 and 892 \u03bcg\/mL for LEO and 738 and 940 \u03bcg\/mL for TEO were determined. Finally, it was observed that, at low doses, there is a synergistic effect between TEO + LEO and EOs + ampicillin; (4) Conclusions: The findings demonstrate that TEO and LEO have an inhibitory effect on ESBL-producing E. coli, suggesting that they are candidates for further studies in the formulation of antibiotics to reduce bacterial resistance to traditional antibiotics.<\/jats:p>","DOI":"10.3390\/microorganisms12081702","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T03:43:28Z","timestamp":1724039008000},"page":"1702","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Lippia origanoides and Thymus vulgaris Essential Oils Synergize with Ampicillin against Extended-Spectrum Beta-Lactamase-Producing Escherichia coli"],"prefix":"10.3390","volume":"12","author":[{"given":"Levi Jafet","family":"Bastida-Ram\u00edrez","sequence":"first","affiliation":[{"name":"Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Toluca C.P. 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3383-9668","authenticated-orcid":false,"given":"Leticia","family":"Buend\u00eda-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Toluca C.P. 50200, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Euridice Ladisu","family":"Mej\u00eda-Argueta","sequence":"additional","affiliation":[{"name":"Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Toluca C.P. 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Sandoval-Cabrera","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Toluca C.P. 50180, Mexico"},{"name":"Laboratorio de Alta Especialidad Hemato-Oncolog\u00eda, Hospital para el Ni\u00f1o, Instituto Materno Infantil del Estado de M\u00e9xico, Toluca C.P. 50170, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Magdalena","family":"Garc\u00eda-Fabila","sequence":"additional","affiliation":[{"name":"Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Toluca C.P. 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio Humberto","family":"Pav\u00f3n-Romero","sequence":"additional","affiliation":[{"name":"Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Toluca C.P. 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Padua-Ahumada","sequence":"additional","affiliation":[{"name":"Centro M\u00e9dico, ISSEMyM, Toluca C.P. 52170, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonnathan Guadalupe","family":"Santill\u00e1n-Ben\u00edtez","sequence":"additional","affiliation":[{"name":"Facultad de Qu\u00edmica, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Toluca C.P. 50120, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,17]]},"reference":[{"key":"ref_1","unstructured":"Madigan, M.T., Guerrero, R., Barrachina, C., and Ruiz Berraquero, F. 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