{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:46:58Z","timestamp":1791499618967,"version":"4.3.4"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,18]],"date-time":"2024-08-18T00:00:00Z","timestamp":1723939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Romanian Executive Agency for Higher Education, Research, Development, and Innovation (UEFISCDI)","award":["PN-III-P4-PCE-2021-1797 (PCE 96\/2022)"],"award-info":[{"award-number":["PN-III-P4-PCE-2021-1797 (PCE 96\/2022)"]}]},{"name":"Romanian Executive Agency for Higher Education, Research, Development, and Innovation (UEFISCDI)","award":["ERANET 243\/2021"],"award-info":[{"award-number":["ERANET 243\/2021"]}]},{"name":"Romanian Executive Agency for Higher Education, Research, Development, and Innovation (UEFISCDI)","award":["PN-III-P1-1.1-TE-2021-1515 (TE112\/2022)"],"award-info":[{"award-number":["PN-III-P1-1.1-TE-2021-1515 (TE112\/2022)"]}]},{"name":"Romanian Executive Agency for Higher Education, Research, Development, and Innovation (UEFISCDI)","award":["PN-III-P1-1.1-PD-2021-0540 (PD102\/2022)"],"award-info":[{"award-number":["PN-III-P1-1.1-PD-2021-0540 (PD102\/2022)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Background: This study provides a comprehensive analysis of Acinetobacter baumannii in aquatic environments and fish microbiota by integrating culture-dependent methods, 16S metagenomics, and antibiotic resistance profiling. Methods: A total of 83 A. baumannii isolates were recovered using culture-dependent methods from intra-hospital infections (IHI) and wastewater (WW) and surface water (SW) samples from two southern Romanian cities in August 2022. The antibiotic susceptibility was screened using disc diffusion, microdilution, PCR, and Whole Genome Sequencing assays. Results: The highest microbial load in the analyzed samples was found in Glina, Bucharest, for both WW and SW samples across all investigated phenotypes. For Bucharest isolates, the resistance levels corresponded to fluoroquinolones &gt; aminoglycosides &gt; \u03b2-lactam antibiotics. In contrast, A. baumannii from upstream SW samples in T\u00e2rgovi\u0219te showed the highest resistance to aminoglycosides. The blaOXA-23 gene was frequently detected in IHI, WW, and SW isolates in Bucharest, but was absent in T\u00e2rgovi\u0219te. Molecular phylogeny revealed the presence of ST10 in T\u00e2rgovi\u0219te isolates and ST2 in Bucharest isolates, while other minor STs were not specifically correlated with a sampling point. Using 16S rRNA sequencing, significant differences in microbial populations between the two locations was identified. The low abundance of Alphaproteobacteria and Actinobacteria in both locations suggests environmental pressures or contamination events. Conclusions: These findings indicate significant fecal contamination and potential public health risks, emphasizing the need for improved water quality monitoring and management.<\/jats:p>","DOI":"10.3390\/microorganisms12081703","type":"journal-article","created":{"date-parts":[[2024,8,19]],"date-time":"2024-08-19T03:43:28Z","timestamp":1724039008000},"page":"1703","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Tracing Acinetobacter baumannii\u2019s Journey from Hospitals to Aquatic Ecosystems"],"prefix":"10.3390","volume":"12","author":[{"given":"Irina","family":"Gheorghe-Barbu","sequence":"first","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1\u20133, 060101 Bucharest, Romania"},{"name":"The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rares-Ionut","family":"Dragomir","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1\u20133, 060101 Bucharest, Romania"},{"name":"The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6384-1822","authenticated-orcid":false,"given":"Gratiela","family":"Gradisteanu Pircalabioru","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1\u20133, 060101 Bucharest, Romania"},{"name":"The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3218-4644","authenticated-orcid":false,"given":"Marius","family":"Surleac","sequence":"additional","affiliation":[{"name":"The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania"},{"name":"National Institute for Infectious Diseases, \u201cMatei Bal\u0219\u201d, Dr. Calistrat Grozovici No. 1, 021105 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iulia Adelina","family":"Dinu","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1\u20133, 060101 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4196-8160","authenticated-orcid":false,"given":"Madalina Diana","family":"Gaboreanu","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1\u20133, 060101 Bucharest, Romania"},{"name":"The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilda","family":"Czobor Barbu","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Bucharest, Intr. Portocalelor No. 1\u20133, 060101 Bucharest, Romania"},{"name":"The Research Institute of the University of Bucharest (ICUB), B.P Hasdeu No. 7, 050095 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Drane, K., Sheehan, M., Whelan, A., Ariel, E., and Kinobe, R. (2024). The Role of Wastewater Treatment Plants in Dissemination of Antibiotic Resistance: Source, Measurement, Removal and Risk Assessment. Antibiotics, 13.","DOI":"10.3390\/antibiotics13070668"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/tbed.12145","article-title":"The Components of \u201cOne World\u2014One Health\u201d Approach","volume":"60","author":"Calistri","year":"2013","journal-title":"Transbound. Emerg. 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