{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:06:55Z","timestamp":1790838415340,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,31]],"date-time":"2024-08-31T00:00:00Z","timestamp":1725062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National College Students Innovation and Entrepreneurship Training Program","award":["202210626005"],"award-info":[{"award-number":["202210626005"]}]},{"name":"National College Students Innovation and Entrepreneurship Training Program","award":["31972722"],"award-info":[{"award-number":["31972722"]}]},{"name":"National College Students Innovation and Entrepreneurship Training Program","award":["2021ZDZX0010"],"award-info":[{"award-number":["2021ZDZX0010"]}]},{"name":"National Natural Science Foundation of China","award":["202210626005"],"award-info":[{"award-number":["202210626005"]}]},{"name":"National Natural Science Foundation of China","award":["31972722"],"award-info":[{"award-number":["31972722"]}]},{"name":"National Natural Science Foundation of China","award":["2021ZDZX0010"],"award-info":[{"award-number":["2021ZDZX0010"]}]},{"name":"\u201c14th Five-Year\u201d Swine Industry Major Science and Technology Special Project of Sichuan Province","award":["202210626005"],"award-info":[{"award-number":["202210626005"]}]},{"name":"\u201c14th Five-Year\u201d Swine Industry Major Science and Technology Special Project of Sichuan Province","award":["31972722"],"award-info":[{"award-number":["31972722"]}]},{"name":"\u201c14th Five-Year\u201d Swine Industry Major Science and Technology Special Project of Sichuan Province","award":["2021ZDZX0010"],"award-info":[{"award-number":["2021ZDZX0010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Although the Japanese encephalitis virus (JEV) infects various cell types, its receptor molecules are still not clearly understood. In our laboratory\u2019s prior research, Rab4b was identified as a potential host factor that facilitates JEV infection in PK15 cells, utilizing a genome-wide CRISPR\/Cas9 knockout library (PK-15-GeCKO). To further explore the effect of Rab4b on JEV replication, we used the Rab4b knockout PK15 cell line using the CRISPR\/Cas9 technology and overexpressing the Rab4b PK15 cell line, with IFA, RT\u2013qPCR, and Western blot to study the effect of Rab4b on viral replication in the whole life cycle of the JEV. The results show that the knockout of Rab4b inhibited the replication of the JEV in PK15 cells, and the overexpression of Rab4b promoted the replication of the JEV in PK15 cell lines. Furthermore, we demonstrated for the first time that host factor Rab4b facilitates the adsorption, internalization, assembly, and release of the JEV, thereby promoting JEV replication. This study enriches the regulatory network between the JEV and host factors and lays the experimental foundation for further understanding of the function of the Rab4b protein.<\/jats:p>","DOI":"10.3390\/microorganisms12091804","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T03:45:21Z","timestamp":1725248721000},"page":"1804","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Host Factor Rab4b Promotes Japanese Encephalitis\u00a0Virus Replication"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3184-2932","authenticated-orcid":false,"given":"Qin","family":"Zhao","sequence":"first","affiliation":[{"name":"Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China"},{"name":"National Demonstration Center for Experimental Animal Education, Sichuan Agricultural University, Chengdu 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