{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T23:39:42Z","timestamp":1793403582801,"version":"4.5.0"},"reference-count":72,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation M\u00e9diterran\u00e9e Infection","award":["Reference: 10-IAHU-03"],"award-info":[{"award-number":["Reference: 10-IAHU-03"]}]},{"name":"Rihane Riyane","award":["Reference: 10-IAHU-03"],"award-info":[{"award-number":["Reference: 10-IAHU-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>The current study aimed to determine the molecular mechanisms of carbapenem and colistin resistance among the clinical isolates of Klebsiella pneumoniae from hospitalized patients admitted to a university hospital in Eastern Algeria. In total, 124 non-duplicate isolates of K. pneumoniae were collected from September 2018 to April 2019. Bacterial identification was performed using MALDI-TOF MS. The presence of extended spectrum \u03b2-lactamase (ESBL) genes, carbapenemase genes, chromosomal mutation and mcr genes in colistin-resistant K. pneumoniae were evaluated by PCR. ESBLs represented a rate of 49.1% and harbored blaCTX-M, blaTEM and blaSHV genes. Concerning carbapenems, 12 strains (9.6%) were resistant to ertapenem (MIC: 1\u201332 \u03bcg\/mL), of which one strain (0.8%) was also resistant to imipenem (MIC: 32 \u03bcg\/mL). Among these strains, nine (75%) harbored blaOXA-48 gene. Seven strains (5.6%) expressed resistance to colistin (MIC: 2\u201332 \u03bcg\/mL), of which two harbored mcr-8 and mgrB genes simultaneously. The existence of a double resistance to colistin in the same strain is new in Algeria, and this could raise concerns about the increase in levels of resistance to this antibiotic (MIC: 32 \u03bcg\/mL). The mgrB gene alone was observed in five isolates (71.4%), including two strains harboring blaOXA-48. This is the first report revealing the presence of K. pneumoniae strains carrying the blaOXA-48 gene as well as a mutation in the mgrB gene. Large-scale surveillance and effective infection control measures are also urgently needed to prevent the outbreak of various carbapenem- and colistin-resistant isolates.<\/jats:p>","DOI":"10.3390\/microorganisms12101942","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T04:05:46Z","timestamp":1727323546000},"page":"1942","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Characterization of Carbapenem and Colistin Resistance in Klebsiella pneumoniae Isolates Obtained from Clinical Samples at a University Hospital Center in Algeria"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3508-4232","authenticated-orcid":false,"given":"Riyane","family":"Rihane","sequence":"first","affiliation":[{"name":"Molecular and Cellular Biology Laboratory, University of Mentouri Brothers Constantine 1, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9993-0408","authenticated-orcid":false,"given":"Abla","family":"Hecini-Hannachi","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Medicine, University of Salah Boubnider Constantine 3, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chafia","family":"Bentchouala","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Medicine, University of Salah Boubnider Constantine 3, Constantine 25000, Algeria"},{"name":"Bacteriology Laboratory, Benbadis University Hospital, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaddour","family":"Benlabed","sequence":"additional","affiliation":[{"name":"Department of Medicine, Faculty of Medicine, University of Salah Boubnider Constantine 3, Constantine 25000, Algeria"},{"name":"Bacteriology Laboratory, Benbadis University Hospital, Constantine 25000, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6466-2324","authenticated-orcid":false,"given":"Seydina M.","family":"Diene","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infections (MEPHI), Institut de Recherche pour le D\u00e9veloppement (IRD), Assistance Publique-Hopitaux de Marseille (AP-HM), IHU-M\u00e9diterran\u00e9e Infection, Facult\u00e9 de Pharmacie, Aix-Marseille University, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/ese.13.47.19044-en","article-title":"Increasing Prevalence of ESBL-Producing Enterobacteriaceae in Europe","volume":"13","author":"Coque","year":"2008","journal-title":"Eurosurveillance"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3201\/eid1710.110655","article-title":"Global Spread of Carbapenemase Producing Enterobacteriaceae","volume":"17","author":"Nordmann","year":"2011","journal-title":"Emerg. 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