{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:56:03Z","timestamp":1791878163047,"version":"4.3.4"},"reference-count":43,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NSERC","award":["06912"],"award-info":[{"award-number":["06912"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Zero-valent copper and silver metals (Ms) nanoparticles (NPs) supported on carboxymethylcellulose (CMC) were synthesized for treating Enterotoxigenic Escherichia coli fimbriae 4 (ETEC:F4), a major cause of diarrhea in post-weaned pigs. The antibacterial properties of Cu0\/CMC and Ag0\/CMC were assessed on infected porcine intestinal enterocyte IPEC-J2, an in vitro model mimicking the small intestine. The lower average particle size (218 nm) and polydispersity index [PDI]: 0.25) for Ag0\/CMC, when compared with those of Cu0\/CMC (367 nm and PDI 0.96), were explained by stronger Ag0\/CMC interactions. The minimal inhibitory concentration (MIC) and half inhibitory concentration (IC50) of Ag0\/CMC were lower in both bacteria and IPEC-J2 cells than those of Cu0\/CMC, confirming that silver nanoparticles are more bactericidal than copper counterparts. IPEC-J2, less sensitive in MNP\/CMC treatment, was used to further investigate the infective process by ETEC:F4. The IC50 of MNP\/CMC increased significantly when infected IPEC-J2 cells and ETEC were co-treated, showing an inhibition of the cytotoxicity effect of ETEC:F4 infection and protection of treated IPEC-J2. Thus, it appears that metal insertion in CMC induces an inhibiting effect on ETEC:F4 growth and that MNP\/CMC dispersion governs the enhancement of this effect. These results open promising prospects for metal-loaded biopolymers for preventing and treating swine diarrhea.<\/jats:p>","DOI":"10.3390\/microorganisms12102026","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T07:08:18Z","timestamp":1728284898000},"page":"2026","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Silver and Copper Nanoparticles Hosted by Carboxymethyl Cellulose Reduce the Infective Effects of Enterotoxigenic Escherichia coli:F4 on Porcine Intestinal Enterocyte IPEC-J2"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6518-7011","authenticated-orcid":false,"given":"Armelle","family":"Tchoumi Neree","sequence":"first","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"},{"name":"Swine and Poultry Infectious Research Center (CRIPA-FRQNT), Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzaneh","family":"Noori","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al, Montr\u00e9al, QC H3C 3P8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7968-9634","authenticated-orcid":false,"given":"Abdelkrim","family":"Azzouz","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al, Montr\u00e9al, QC H3C 3P8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5067-7501","authenticated-orcid":false,"given":"Marcio","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"},{"name":"Swine and Poultry Infectious Research Center (CRIPA-FRQNT), Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7163-1756","authenticated-orcid":false,"given":"John Morris","family":"Fairbrother","sequence":"additional","affiliation":[{"name":"Swine and Poultry Infectious Research Center (CRIPA-FRQNT), Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"},{"name":"World Organisation for Animal Health (OIE), Reference Laboratory for <i>Escherichia coli<\/i> (EcL), Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5922-4701","authenticated-orcid":false,"given":"Mircea Alexandru","family":"Mateescu","sequence":"additional","affiliation":[{"name":"Swine and Poultry Infectious Research Center (CRIPA-FRQNT), Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"},{"name":"Department of Chemistry, Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al, Montr\u00e9al, QC H3C 3P8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7893-9649","authenticated-orcid":false,"given":"Younes","family":"Chorfi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"},{"name":"Swine and Poultry Infectious Research Center (CRIPA-FRQNT), Faculty of Veterinary Medicine, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, QC J2S 2M2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.vetmic.2007.02.018","article-title":"Prevalence of virulence genes in Escherichia coli strains recently isolated from young pigs with diarrhea in the US","volume":"123","author":"Zhang","year":"2007","journal-title":"Vet. 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