{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T12:34:42Z","timestamp":1777898082623,"version":"3.51.4"},"reference-count":59,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T00:00:00Z","timestamp":1729900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Canadian Urological Association scholarship fund","award":["#258229"],"award-info":[{"award-number":["#258229"]}]},{"name":"Canadian Institutes of Health Research","award":["#258229"],"award-info":[{"award-number":["#258229"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Uropathogenic Escherichia coli are the main causal agent of urinary tract infections. These diseases can affect more than half of women during their lifetime. Moreover, recurrent urinary tract infections can affect up to 30% of patients, leading to higher social and economic costs for the community. No efficient treatment against the recurrent form of the disease has been discovered. Due to the low average rate of successful translation from 2D cell culture and in vivo animal models into clinical trials, new models that mimic pathologies, such as those produced by tissue engineering, are needed. A model of human-derived 3D bladder mucosa was produced by tissue engineering techniques using collagen gels and organ-specific primary human stromal and epithelial cell populations. This model was used to mimic the different steps of a urinary tract infection: adhesion, invasion, intracellular bacterial community and quiescent intracellular reservoir formation and, finally, bacteria resurgence after umbrella cell exfoliation through chitosan exposure to mimic the recurrent infection. The uropathogenic strain UTI-89-GFP was used as infectious bacteria and BL-21-GFP strain as a control. Our model is unique and is the first step toward mimicking the different phases of a UTI in a human context.<\/jats:p>","DOI":"10.3390\/microorganisms12112155","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T05:07:22Z","timestamp":1730092042000},"page":"2155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mimicking Urinary Tract Infections Caused by Uropathogenic Escherichia coli Using a Human Three-Dimensional Tissue Engineering Model"],"prefix":"10.3390","volume":"12","author":[{"given":"F\u00e9lix-Antoine","family":"Pellerin","sequence":"first","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Quebec, QC G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9lodie","family":"Dufresne","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Quebec, QC G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0389-667X","authenticated-orcid":false,"given":"St\u00e9phane","family":"Chabaud","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Quebec, QC G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6606-3168","authenticated-orcid":false,"given":"Hazem","family":"Orabi","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Quebec, QC G1J 1Z4, Canada"},{"name":"Department of Urology, Assiut University, Assiut 71515, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6680-5563","authenticated-orcid":false,"given":"St\u00e9phane","family":"Bolduc","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Center, Quebec, QC G1J 1Z4, Canada"},{"name":"Department of Surgery, Faculty of Medicine, Laval University, Quebec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1038\/nrurol.2010.190","article-title":"The epidemiology of urinary tract infection","volume":"7","author":"Foxman","year":"2010","journal-title":"Nat. Rev. Urol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1093\/oxfordjournals.aje.a010170","article-title":"Risk factors for second urinary tract infection among college women","volume":"151","author":"Foxman","year":"2000","journal-title":"Am. J. Epidemiol."},{"key":"ref_3","first-page":"451","article-title":"Diagnosis and management of uncomplicated urinary tract infections","volume":"72","year":"2005","journal-title":"Am. Fam. Physician"},{"key":"ref_4","first-page":"1","article-title":"2006 National Hospital Discharge Survey","volume":"5","author":"DeFrances","year":"2008","journal-title":"Natl. Health Stat. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1097\/01.ju.0000152365.43125.3b","article-title":"Urologic Diseases in America Project. Urologic diseases in America Project: Analytical methods and principal findings","volume":"173","author":"Litwin","year":"2005","journal-title":"J. Urol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1128\/JCM.44.5.1776-1781.2006","article-title":"Pulsed-field gel electrophoresis typing of Escherichia coli strains from samples collected before and after pivmecillinam or placebo treatment of uncomplicated community-acquired urinary tract infection in women","volume":"44","author":"Ejrnaes","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4572","DOI":"10.1128\/IAI.69.7.4572-4579.2001","article-title":"Establishment of a persistent Escherichia coli reservoir during the acute phase of a bladder infection","volume":"69","author":"Mulvey","year":"2001","journal-title":"Infect. Immun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1093\/cid\/cit284","article-title":"Urinary tract infections: Current and emerging management strategies","volume":"57","author":"Barber","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/nrmicro3432","article-title":"Urinary tract infections: Epidemiology, mechanisms of infection and treatment options","volume":"13","author":"Walker","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.mcna.2013.03.006","article-title":"Management of urinary tract infections in the era of increasing antimicrobial resistance","volume":"97","author":"Shepherd","year":"2013","journal-title":"Med. Clin. North Am."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Murray, B.O., Flores, C., Williams, C., Flusberg, D.A., Marr, E.E., Kwiatkowska, K.M., Charest, J.L., Isenberg, B.C., and Rohn, J.L. (2021). Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.691210"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1078\/0171-9335-00351","article-title":"What determines differentiation of urothelial umbrella cells?","volume":"83","author":"Veranic","year":"2004","journal-title":"Eur. J. Cell Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"13716","DOI":"10.1016\/S0021-9258(17)36889-8","article-title":"Mammalian uroplakins. A group of highly conserved urothelial differentiation-related membrane proteins","volume":"269","author":"Wu","year":"1994","journal-title":"J. Biol. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"F867","DOI":"10.1152\/ajprenal.2000.278.6.F867","article-title":"Everything you wanted to know about the bladder epithelium but were afraid to ask","volume":"278","author":"Lewis","year":"2000","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1111\/j.1574-6976.2012.00339.x","article-title":"Host-pathogen checkpoints and population bottlenecks in persistent and intracellular uropathogenic Escherichia coli bladder infection","volume":"36","author":"Hannan","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"14644","DOI":"10.1074\/jbc.M600877200","article-title":"Distinct glycan structures of uroplakins Ia and Ib: Structural basis for the selective binding of FimH adhesin to uroplakin Ia","volume":"281","author":"Xie","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1046\/j.1462-5822.2002.00166.x","article-title":"Requirement of Rho-family GTPases in the invasion of Type 1-piliated uropathogenic Escherichia coli","volume":"4","author":"Martinez","year":"2002","journal-title":"Cell. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1073\/pnas.0308125100","article-title":"Differentiation and developmental pathways of uropathogenic Escherichia coli in urinary tract pathogenesis","volume":"101","author":"Justice","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1126\/science.1084550","article-title":"Intracellular bacterial biofilm-like pods in urinary tract infections","volume":"301","author":"Anderson","year":"2003","journal-title":"Science"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hannan, T.J., Mysorekar, I.U., Hung, C.S., Isaacson-Schmid, M.L., and Hultgren, S.J. (2010). Early severe inflammatory responses to uropathogenic E. coli predispose to chronic and recurrent urinary tract infection. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1001042"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.tim.2004.07.005","article-title":"Intracellular bacterial communities of uropathogenic Escherichia coli in urinary tract pathogenesis","volume":"12","author":"Anderson","year":"2004","journal-title":"Trends Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"a010306","DOI":"10.1101\/cshperspect.a010306","article-title":"Bacterial biofilms: Development, dispersal, and therapeutic strategies in the dawn of the postantibiotic era","volume":"3","author":"Kostakioti","year":"2013","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7042","DOI":"10.1128\/IAI.70.12.7042-7049.2002","article-title":"Effect of trimethoprim-sulfamethoxazole on recurrent bacteriuria and bacterial persistence in mice infected with uropathogenic Escherichia coli","volume":"70","author":"Schilling","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7412","DOI":"10.1074\/jbc.M110560200","article-title":"Molecular regulation of urothelial renewal and host defenses during infection with uropathogenic Escherichia coli","volume":"277","author":"Mysorekar","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/j.1462-5822.2006.00691.x","article-title":"Actin-gated intracellular growth and resurgence of uropathogenic Escherichia coli","volume":"8","author":"Eto","year":"2006","journal-title":"Cell. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1128\/AAC.44.1.156-163.2000","article-title":"Development of a long-term ascending urinary tract infection mouse model for antibiotic treatment studies","volume":"44","author":"Hvidberg","year":"2000","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1093\/jac\/dki002","article-title":"Intracellular persistence of Escherichia coli in urinary bladders from mecillinam-treated mice","volume":"55","author":"Kerrn","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.ejps.2005.02.003","article-title":"Permeability of pig urinary bladder wall: The effect of chitosan and the role of calcium","volume":"25","author":"Kerec","year":"2005","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/s00418-013-1088-7","article-title":"Correlative study of functional and structural regeneration of urothelium after chitosan-induced injury","volume":"140","author":"Erman","year":"2013","journal-title":"Histochem. Cell Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1128\/MMBR.00067-15","article-title":"Strengths and Limitations of Model Systems for the Study of Urinary Tract Infections and Related Pathologies","volume":"80","author":"Barber","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/978-1-4939-2854-5_14","article-title":"A Murine Model for Escherichia coli Urinary Tract Infection","volume":"1333","author":"Hannan","year":"2016","journal-title":"Bact. Persistence Methods Protoc."},{"key":"ref_32","first-page":"780","article-title":"Urinary tract infection of mice to model human disease: Practicalities, implications and limitations","volume":"42","author":"Carey","year":"2016","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_33","first-page":"114","article-title":"Lost in translation: Animal models and clinical trials in cancer treatment","volume":"6","author":"Mak","year":"2014","journal-title":"Am. J. Transl. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"E65","DOI":"10.1002\/term.1711","article-title":"Lysophosphatidic acid enhances collagen deposition and matrix thickening in engineered tissue","volume":"9","author":"Chabaud","year":"2015","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1002\/term.2036","article-title":"Inexpensive production of near-native engineered stromas","volume":"11","author":"Chabaud","year":"2017","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3090","DOI":"10.1002\/term.2212","article-title":"Urothelial cell expansion and differentiation are improved by exposure to hypoxia","volume":"11","author":"Chabaud","year":"2017","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00345-015-1596-2","article-title":"Organ-specific matrix self-assembled by mesenchymal cells improves the normal urothelial differentiation in vitro","volume":"34","author":"Bouhout","year":"2016","journal-title":"World J. Urol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Eshaghi, M., Mehershahi, K.S., and Chen, S.L. (2016). Brighter Fluorescent Derivatives of UTI89 Utilizing a Monomeric vGFP. Pathogens, 5.","DOI":"10.3390\/pathogens5010003"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1016\/j.juro.2015.03.001","article-title":"A Novel and Faster Method to Obtain a Differentiated 3-Dimensional Tissue Engineered Bladder","volume":"194","author":"Bouhout","year":"2015","journal-title":"J. Urol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.msec.2019.04.052","article-title":"Collagen hollow structure for bladder tissue engineering","volume":"102","author":"Bouhout","year":"2019","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1128\/iai.54.3.613-620.1986","article-title":"Regulation of production of type 1 pili among urinary tract isolates of Escherichia coli","volume":"54","author":"Hultgren","year":"1986","journal-title":"Infect. Immun."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1128\/IAI.66.7.3303-3310.1998","article-title":"In vivo phase variation of Escherichia coli type 1 fimbrial genes in women with urinary tract infection","volume":"66","author":"Lim","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1152\/physrev.00041.2019","article-title":"The Urothelium: Life in a Liquid Environment","volume":"100","author":"Dalghi","year":"2020","journal-title":"Physiol. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1089\/ten.tea.2012.0117","article-title":"Human epithelial stem cells persist within tissue-engineered skin produced by the self-assembly approach","volume":"19","author":"Lavoie","year":"2013","journal-title":"Tissue Eng. Part A"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1128\/AAC.00014-10","article-title":"Persistence of uropathogenic Escherichia coli in the face of multiple antibiotics","volume":"54","author":"Blango","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Blango, M.G., Ott, E.M., Erman, A., Veranic, P., and Mulvey, M.A. (2014). Forced resurgence and targeting of intracellular uropathogenic Escherichia coli reservoirs. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0093327"},{"key":"ref_47","first-page":"375","article-title":"Repeated Treatments with Chitosan in Combination with Antibiotics Completely Eradicate Uropathogenic Escherichia coli from Infected Mouse Urinary Bladders","volume":"216","author":"Erman","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/s00418-011-0857-4","article-title":"Expression and localization of four uroplakins in urothelial preneoplastic lesions","volume":"136","author":"Zhou","year":"2011","journal-title":"Histochem. Cell Biol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s00428-011-1045-6","article-title":"Altered expression of UPIa, UPIb, UPII, and UPIIIa during urothelial carcinogenesis induced by N-butyl-N-(4-hydroxybutyl)nitrosamine in rats","volume":"458","author":"Vidmar","year":"2011","journal-title":"Virchows Arch."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1016\/S0002-9440(10)65709-4","article-title":"Uroplakin gene expression by normal and neoplastic human urothelium","volume":"153","author":"Lobban","year":"1998","journal-title":"Am. J. Pathol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1089\/ten.tec.2020.0333","article-title":"Immunocompetent Human 3D Organ-Specific Hormone-Responding Vaginal Mucosa Model of HIV-1 Infection","volume":"27","author":"Saba","year":"2021","journal-title":"Tissue Eng. Part C Methods"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"100428","DOI":"10.1016\/j.jlr.2023.100428","article-title":"Eicosapentaenoic acid reduces the proportion of IL-17A-producing T cells in a 3D psoriatic skin model","volume":"64","author":"Morin","year":"2023","journal-title":"J. Lipid Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.actbio.2023.06.045","article-title":"An in vitro autologous, vascularized, and immunocompetent Tissue Engineered Skin model obtained by the self-assembled approach","volume":"168","author":"Attiogbe","year":"2023","journal-title":"Acta Biomater."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.jpurol.2011.03.002","article-title":"Bladder substitute reconstructed in a physiological pressure environment","volume":"7","author":"Bouhout","year":"2011","journal-title":"J. Pediatr. Urol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Caneparo, C., Chabaud, S., Fradette, J., and Bolduc, S. (2022). Evaluation of a Serum-Free Medium for Human Epithelial and Stromal Cell Culture. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231710035"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Hassuna, N.A., Rabie, E.M., Mahd, W.K.M., Refaie, M.M., Yousef, R.K.M., and Abdelraheem, W.M. (2023). Antibacterial effect of vitamin C against uropathogenic E. coli in vitro and in vivo. BMC Microbiol., 23.","DOI":"10.1186\/s12866-023-02856-3"},{"key":"ref_57","first-page":"252","article-title":"Uroplakins and their potential applications in urology","volume":"69","author":"Matuszewski","year":"2016","journal-title":"Cent. Eur. J. Urol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1042\/BST0390349","article-title":"Glycosylation changes as important factors for the susceptibility to urinary tract infection","volume":"39","author":"Taganna","year":"2011","journal-title":"Biochem. Soc. Trans."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Roussel, C., Chabaud, S., Lessard-Lord, J., Cattero, V., Pellerin, F.A., Feutry, P., Bochard, V., Bolduc, S., and Desjardins, Y. (2022). UPEC Colonic-Virulence and Urovirulence Are Blunted by Proanthocyanidins-Rich Cranberry Extract Microbial Metabolites in a Gut Model and a 3D Tissue-Engineered Urothelium. Microbiol. Spectr., 10.","DOI":"10.1128\/spectrum.02432-21"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/11\/2155\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:21:12Z","timestamp":1760113272000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/12\/11\/2155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,26]]},"references-count":59,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11]]}},"alternative-id":["microorganisms12112155"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms12112155","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,26]]}}}