{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:58:24Z","timestamp":1792623504594,"version":"4.5.0"},"reference-count":35,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004423","name":"World Health Organization through the Tricycle program","doi-asserted-by":"publisher","award":["APW 2018\/821086"],"award-info":[{"award-number":["APW 2018\/821086"]}],"id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Extended-spectrum \u03b2-lactamase-producing Escherichia coli (ESBL-Ec) poses a significant public health concern due to its widespread prevalence and resistance to multiple antibiotics. This study aimed to assess the prevalence, resistance profile, and carriage of ESBL-encoding genes in ESBL-Ec isolates from broilers in two markets of Dakar, Senegal. Sampling over a one-year period revealed that 61.7% of broilers carried ESBL-Ec in their cecum. Antibiotic susceptibility testing of 186 ESBL-Ec isolates showed high resistance to \u03b2-lactam antibiotics, including ampicillin, ticarcillin, and third-generation cephalosporins. Notably, all isolates were susceptible to imipenem. Multidrug resistance was frequent, with 91.4% of the isolates displaying this phenotype. Comparison between the two markets revealed variations in resistance to quinolones. PCR analysis detected blaCTX-M genes in all isolates, with blaCTX-M-1 being the most prevalent subgroup. Additionally, blaTEM and blaOXA genes were found in 26.3% and 2.7% of isolates, respectively, while no blaSHV genes were detected. Combinations of ESBL genes were common, with blaCTX-M15-blaTEM being the most frequent. These findings highlight the widespread prevalence of ESBL-Ec in broilers from Dakar markets, indicating a potential risk of transmission to humans through the food chain. Effective surveillance and intervention strategies are crucial to mitigate the spread of antimicrobial resistance in humans and animals.<\/jats:p>","DOI":"10.3390\/microorganisms12112357","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T04:53:05Z","timestamp":1731991985000},"page":"2357","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Prevalence, Antimicrobial Susceptibility, and Resistance Genes of Extended-Spectrum \u03b2-Lactamase-Producing Escherichia coli from Broilers Sold in Open Markets of Dakar, Senegal"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7845-2800","authenticated-orcid":false,"given":"Abdoulaye","family":"Ciss\u00e9","sequence":"first","affiliation":[{"name":"P\u00f4le de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal"},{"name":"Laboratoire de Bact\u00e9riologie-Virologie, H\u00f4pital Aristide le Dantec, Dakar BP 3001, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bissoume","family":"Sambe Ba","sequence":"additional","affiliation":[{"name":"P\u00f4le de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9338-6544","authenticated-orcid":false,"given":"Ousmane","family":"Sow","sequence":"additional","affiliation":[{"name":"P\u00f4le de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdoul Aziz","family":"Wane","sequence":"additional","affiliation":[{"name":"P\u00f4le de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8918-187X","authenticated-orcid":false,"given":"Issa","family":"Ndiaye","sequence":"additional","affiliation":[{"name":"P\u00f4le de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5405-2824","authenticated-orcid":false,"given":"Cheikh","family":"Fall","sequence":"additional","affiliation":[{"name":"P\u00f4le de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makhtar","family":"Camara","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie-Virologie, H\u00f4pital Aristide le Dantec, Dakar BP 3001, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2461-6558","authenticated-orcid":false,"given":"Yakhya","family":"Dieye","sequence":"additional","affiliation":[{"name":"P\u00f4le de Microbiologie, Institut Pasteur de Dakar, Dakar BP 220, Senegal"},{"name":"Groupe de Recherche Biotechnologies Appliqu\u00e9es & Bioproc\u00e9d\u00e9s Environnementaux (GRBA-BE), \u00c9cole Sup\u00e9rieure Polytechnique, Universit\u00e9 Cheikh Anta Diop, Dakar BP 5085, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Geser, N., Stephan, R., and H\u00e4chler, H. 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