{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:29:35Z","timestamp":1791894575136,"version":"4.3.4"},"reference-count":37,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T00:00:00Z","timestamp":1734307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Colciencias","doi-asserted-by":"publisher","award":["120480763040"],"award-info":[{"award-number":["120480763040"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007637","name":"Colciencias","doi-asserted-by":"publisher","award":["130880764152 CT776-2018"],"award-info":[{"award-number":["130880764152 CT776-2018"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Minciencias","award":["120480763040"],"award-info":[{"award-number":["120480763040"]}]},{"name":"Minciencias","award":["130880764152 CT776-2018"],"award-info":[{"award-number":["130880764152 CT776-2018"]}]},{"name":"Vice Presidency for the Research &amp; Creation","award":["120480763040"],"award-info":[{"award-number":["120480763040"]}]},{"name":"Vice Presidency for the Research &amp; Creation","award":["130880764152 CT776-2018"],"award-info":[{"award-number":["130880764152 CT776-2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Staphylococcus aureus is a human pathogen responsible for a wide range of diseases, such as skin and soft tissue infections, pneumonia, toxic shock syndrome, and urinary tract infections. Methicillin-resistant S. aureus (MRSA) is a well-known pathogen with consistently high mortality rates. Detecting the mecA resistance gene and phenotypical profile to \u03b2-lactams allows for the differentiation of MRSA from methicillin-susceptible S. aureus (MSSA) isolates. In this study, we characterized 57 S. aureus clinical isolates for \u03b2-lactam susceptibility and mecA presence. We classified 52.63% as MRSA and 45.61% as MSSA. However, some isolates evidenced different oxacillin resistance profiles, such as borderline oxacillin-resistant or modified S. aureus (BORSA\/MODSA). The cefazolin inoculum effect (CzIE) was established for these samples, emphasizing the relevance of these isolates as a source of therapeutic failure. We also performed the detection of the Panton-Valentine Leucocidin virulence genes as well as the S. aureus spa-type clonality. As expected, spa-types t002 and t008 were the most prevalent clones, demonstrating the success of well-established clones. These findings emphasize the importance of establishing sensitivity profiles, especially in isolates with poor resistance mechanisms, to determine their prevalence and their impact on public health.<\/jats:p>","DOI":"10.3390\/microorganisms12122598","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T09:17:58Z","timestamp":1734340678000},"page":"2598","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Discordant \u03b2-Lactam Susceptibility in Clinical Staphylococcus aureus Isolates: A Molecular and Phenotypical Exploration to Detect the BORSA\/MODSA Isolates in Bogot\u00e1, Colombia"],"prefix":"10.3390","volume":"12","author":[{"given":"Angie","family":"Fonseca-Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n Celular y Molecular de Microorganismos Pat\u00f3genos, Department of Biological Scieces, Universidad de los Andes, Bogot\u00e1 111711, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Mancera-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Celular y Molecular de Microorganismos Pat\u00f3genos, Department of Biological Scieces, Universidad de los Andes, Bogot\u00e1 111711, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aura","family":"Leal-Castro","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Enfermedades Infecciosas, Faculty of Medicine, Universidad Nacional de Colombia, Bogot\u00e1 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6331-8399","authenticated-orcid":false,"given":"Chad","family":"Leidy","sequence":"additional","affiliation":[{"name":"Biophysics Group, Department of Physics, Universidad de los Andes, Bogot\u00e1 111711, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Rinc\u00f3n","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00e9tica y Resistencia Antimicrobiana, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Carvajal","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00e9tica y Resistencia Antimicrobiana, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinnethe","family":"Reyes","sequence":"additional","affiliation":[{"name":"Unidad de Gen\u00e9tica y Resistencia Antimicrobiana, Universidad El Bosque, Bogot\u00e1 110121, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3057-1966","authenticated-orcid":false,"given":"Adriana","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Celular y Molecular de Microorganismos Pat\u00f3genos, Department of Biological Scieces, Universidad de los Andes, Bogot\u00e1 111711, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,16]]},"reference":[{"key":"ref_1","unstructured":"Pourmand, M.R., Hassanzadeh, S., Mashhadi, R., and Askari, E. 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