{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T23:43:15Z","timestamp":1782430995371,"version":"3.54.5"},"reference-count":53,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T00:00:00Z","timestamp":1736294400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","award":["272983813"],"award-info":[{"award-number":["272983813"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","award":["TTU 05.820_02"],"award-info":[{"award-number":["TTU 05.820_02"]}]},{"name":"German Center for Infection Research (DZIF)","award":["272983813"],"award-info":[{"award-number":["272983813"]}]},{"name":"German Center for Infection Research (DZIF)","award":["TTU 05.820_02"],"award-info":[{"award-number":["TTU 05.820_02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Viral meningitis poses a significant clinical challenge due to its rapid onset and potential progression to life-threatening encephalitis. Early detection of treatable viral pathogens such as Herpes simplex virus (HSV), Cytomegalovirus (CMV), and Varicella-zoster virus (VZV) is essential for initiating appropriate therapies. However, multiplex PCRs for the rapid and simultaneous detection of these pathogens are scarce due to the complex PCR design and the elaborate validation process using cerebrospinal fluid samples. In this study, we established and validated a novel multiplex PCR assay for detecting HSV, CMV, and VZV in cerebrospinal fluid samples and implemented the assay on a fully automated platform.<\/jats:p>","DOI":"10.3390\/microorganisms13010111","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T04:54:08Z","timestamp":1736312048000},"page":"111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Multiplex Polymerase Chain Reaction Assay for the Detection of Herpes Simplex Virus, Cytomegalovirus, and Varicella-Zoster Virus in Cerebrospinal Fluid"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2578-8880","authenticated-orcid":false,"given":"Tatjana","family":"Luzius","sequence":"first","affiliation":[{"name":"School of Medicine and Health, Institute of Virology, Technical University of Munich\/Helmholtz Munich, 81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1948-2057","authenticated-orcid":false,"given":"Samuel D.","family":"Jeske","sequence":"additional","affiliation":[{"name":"School of Medicine and Health, Institute of Virology, Technical University of Munich\/Helmholtz Munich, 81675 Munich, Germany"},{"name":"German Center for Infection Research (DZIF), Munich Partner Site, 81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Baer","sequence":"additional","affiliation":[{"name":"QIAGEN GmbH, Strategic Lab Consultancy, 40724 Hilden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uta","family":"Goelnitz","sequence":"additional","affiliation":[{"name":"QIAGEN GmbH, Strategic Lab Consultancy, 40724 Hilden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9421-1911","authenticated-orcid":false,"given":"Ulrike","family":"Protzer","sequence":"additional","affiliation":[{"name":"School of Medicine and Health, Institute of Virology, Technical University of Munich\/Helmholtz Munich, 81675 Munich, Germany"},{"name":"German Center for Infection Research (DZIF), Munich Partner Site, 81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4101-9280","authenticated-orcid":false,"given":"Jochen M.","family":"Wettengel","sequence":"additional","affiliation":[{"name":"School of Medicine and Health, Institute of Virology, Technical University of Munich\/Helmholtz Munich, 81675 Munich, Germany"},{"name":"German Center for Infection Research (DZIF), Munich Partner Site, 81675 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1056\/NEJMoa040845","article-title":"Clinical features and prognostic factors in adults with bacterial meningitis","volume":"351","author":"Spanjaard","year":"2004","journal-title":"N. 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