{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:18:13Z","timestamp":1780719493513,"version":"3.54.1"},"reference-count":108,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["RPLD.01.02.02-10-0029\/18-0"],"award-info":[{"award-number":["RPLD.01.02.02-10-0029\/18-0"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Since 2022, the European Union has banned the use of antibiotics in animal production. We conducted studies to characterize Lactiplantibacillus plantarum (47, AN8, and OK-B) and Ligilactobacillus salivarius (AN9) and evaluate their potential to create a biopreparation based on fermented whey for chickens. The following methods were used: lactic acid bacteria (LAB) culture and storage, crystal violet staining, Koch\u2019s plate method, Caco-2 cell culture, hydrophobicity test, and spectrophotometric measurements. All bacteria showed weak adhesion to polystyrene and collagen, and the L. plantarum species demonstrated weak adhesion to mucus. All bacteria showed strong adhesion to the intestinal epithelial cell line Caco-2. LAB showed strong autoaggregation and coaggregation with E. coli ATCC10536. The highest affinity for xylene was exhibited by L. salivarius AN9 (above 30%) while, for chloroform, the highest affinity was exhibited by L. plantarum OK-B (approx. 95%); the affinity for n-hexadecane for all strains was below 20%. The highest survival in the presence of bile salts (0.3%) was demonstrated by L. plantarum 47 (above 54%). The effect of low pH resulted in decreased viability for all strains. Significant differences were demonstrated in the concentration of lactic acid between MRS and whey medium after culturing LAB. These results will aid in qualifying these strains for further research to create a functional feed for chickens.<\/jats:p>","DOI":"10.3390\/microorganisms13020317","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T08:47:57Z","timestamp":1738572477000},"page":"317","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Characterization of Lactic Acid Bacteria Strains as Components of a Biopreparation for Chickens for Slaughter"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5618-529X","authenticated-orcid":false,"given":"Daria","family":"Zamojska","sequence":"first","affiliation":[{"name":"Polwet Sp. z o.o., M. Konopnickiej 21, 98-100 Lask, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Rosicka-Kaczmarek","sequence":"additional","affiliation":[{"name":"Institute of Food Technology and Analysis, Faculty of Biotechnology and Food Science, Lodz University of Technology, Stefanowskiego 2\/22, 90-530 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Macierzy\u0144ska-Piotrowska","sequence":"additional","affiliation":[{"name":"Polwet Sp. z o.o., M. Konopnickiej 21, 98-100 Lask, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1865-4833","authenticated-orcid":false,"given":"Adriana","family":"Nowak","sequence":"additional","affiliation":[{"name":"Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171\/173, 90-530 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1146\/annurev-resource-100814-125015","article-title":"Economics of Antibiotic Growth Promoters in Livestock","volume":"7","author":"Teillant","year":"2015","journal-title":"Annu. Rev. Resour. Econ. 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