{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:52:23Z","timestamp":1791244343093,"version":"4.1.0"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence Fran\u00e7aise du Developpement (AFD)","award":["N\u00b0 CZZ 2146 01 (ECOMORE 2)"],"award-info":[{"award-number":["N\u00b0 CZZ 2146 01 (ECOMORE 2)"]}]},{"name":"Agence Fran\u00e7aise du Developpement (AFD)","award":["HDTRA121C0034"],"award-info":[{"award-number":["HDTRA121C0034"]}]},{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency (DTRA)","doi-asserted-by":"publisher","award":["N\u00b0 CZZ 2146 01 (ECOMORE 2)"],"award-info":[{"award-number":["N\u00b0 CZZ 2146 01 (ECOMORE 2)"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency (DTRA)","doi-asserted-by":"publisher","award":["HDTRA121C0034"],"award-info":[{"award-number":["HDTRA121C0034"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Dengue fever is a widespread mosquito-borne viral disease caused by infections with dengue virus (DENV). Since its initial detection in 1979, the disease has posed a significant public health threat to the Lao People\u2019s Democratic Republic (Lao PDR). Surveillance is crucial for understanding the circulation of DENV in endemic regions and identifying potential hot spots with higher-than-expected case numbers of dengue fever. In this study, we present the results from our surveillance activities in the Lao PDR spanning 2020\u20132023. While quarantine restrictions from the COVID-19 pandemic posed substantial disruptions to performing DENV surveillance, over 8800 samples were tested during this period, with a positive rate of close to 60%. Cases were reported from all three regions (northern, Central, and southern) of the Lao PDR. Three circulating serotypes (DENV-1, DENV-2, and DENV-4) were detected, with DENV-1 dominant in 2021 and 2022, while DENV-2 was dominant in 2020 and 2023. Phylogenetic analyses suggest that the genotypes of DENV-1, DENV-2, and DENV-4 were closely related to corresponding isolates from neighboring countries. These findings provide an update on the nature of DENV cases detected in the Lao PDR and underscore the critical importance of sustaining a robust surveillance network to track infections.<\/jats:p>","DOI":"10.3390\/microorganisms13020318","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T08:47:57Z","timestamp":1738572477000},"page":"318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular Epidemiology of Dengue Viruses in Lao People\u2019s Democratic Republic, 2020\u20132023"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9173-4525","authenticated-orcid":false,"given":"C\u00e9cile","family":"Troupin","sequence":"first","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kedkeo","family":"Intavong","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somphavanh","family":"Somlor","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souksakhone","family":"Viengphouthong","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sitsana","family":"Keosenhom","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thep Aksone","family":"Chindavong","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phaithong","family":"Bounmany","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longthor","family":"Vachouaxiong","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0011-2200","authenticated-orcid":false,"given":"Thonglakhone","family":"Xaybounsou","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chittaphone","family":"Vanhnollat","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phonepadith","family":"Khattignavong","sequence":"additional","affiliation":[{"name":"Parasitology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darouny","family":"Phonekeo","sequence":"additional","affiliation":[{"name":"Administration Department, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bouaphanh","family":"Khamphaphongphane","sequence":"additional","affiliation":[{"name":"National Center for Laboratory and Epidemiology, Ministry of Health, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phonepadith","family":"Xangsayarath","sequence":"additional","affiliation":[{"name":"Department of Communicable Disease Control, Ministry of Health, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3173-4053","authenticated-orcid":false,"given":"Vincent","family":"Lacoste","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1372-3008","authenticated-orcid":false,"given":"Philippe","family":"Buchy","sequence":"additional","affiliation":[{"name":"Administration Department, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9044-8153","authenticated-orcid":false,"given":"Gary","family":"Wong","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, Laos"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,1]]},"reference":[{"key":"ref_1","unstructured":"WHO (2024, October 23). Dengue and Severe Dengue. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/dengue-and-severe-dengue."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Messina, J.P., Brady, O.J., Golding, N., Kraemer, M.U.G., Wint, G.R.W., Ray, S.E., Pigott, D.M., Shearer, F.M., Johnson, K., and Earl, L. (2019). The Current and Future Global Distribution and Population at Risk of Dengue. Nat. Microbiol., 4.","DOI":"10.1038\/s41564-019-0476-8"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3","DOI":"10.2149\/tmh.2011-S05","article-title":"Dengue, Urbanization and Globalization: The Unholy Trinity of the 21(St) Century","volume":"39","author":"Gubler","year":"2011","journal-title":"Trop. Med. Health"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Harapan, H., Michie, A., Sasmono, R.T., and Imrie, A. (2020). Dengue: A Minireview. Viruses, 12.","DOI":"10.3390\/v12080829"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S0140-6736(14)60572-9","article-title":"Dengue","volume":"385","author":"Guzman","year":"2015","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s00705-023-05835-1","article-title":"Renaming of the Genus Flavivirus to Orthoflavivirus and Extension of Binomial Species Names within the Family Flaviviridae","volume":"168","author":"Postler","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Chen, R., and Vasilakis, N. (2011). Dengue\u2014Quo Tu et Quo Vadis?. Viruses, 3.","DOI":"10.3390\/v3091562"},{"key":"ref_8","first-page":"279","article-title":"Understanding Dengue Virus Evolution to Support Epidemic Surveillance and Counter-Measure Development","volume":"62","author":"Pollett","year":"2018","journal-title":"Infect. Genet. Evol. J. Mol. Epidemiol. Evol. Genet. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S1567-1348(03)00004-2","article-title":"The Origin, Emergence and Evolutionary Genetics of Dengue Virus","volume":"3","author":"Holmes","year":"2003","journal-title":"Infect. Genet. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/S0065-3527(03)59009-1","article-title":"Microevolution and Virulence of Dengue Viruses","volume":"59","year":"2003","journal-title":"Adv. Virus Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1093\/oxfordjournals.aje.a113932","article-title":"Risk Factors in Dengue Shock Syndrome: A Prospective Epidemiologic Study in Rayong, Thailand. I. The 1980 Outbreak","volume":"120","author":"Sangkawibha","year":"1984","journal-title":"Am. J. Epidemiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1093\/infdis\/140.4.527","article-title":"In Vivo Enhancement of Dengue Virus Infection in Rhesus Monkeys by Passively Transferred Antibody","volume":"140","author":"Halstead","year":"1979","journal-title":"J. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gubler, D.J. (1998). Dengue and Dengue Hemorrhagic Fever. Clin. Microbiol. Rev., 11.","DOI":"10.1128\/CMR.11.3.480"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Phadungsombat, J., Nakayama, E.E., and Shioda, T. (2024). Unraveling Dengue Virus Diversity in Asia: An Epidemiological Study through Genetic Sequences and Phylogenetic Analysis. Viruses, 16.","DOI":"10.3390\/v16071046"},{"key":"ref_15","unstructured":"Halstead, S.B. (1965). Dengue and Hemorrhagic Fevers of Southeast Asia. Yale J. Biol. Med., 37."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Shepard, D.S., Undurraga, E.A., and Halasa, Y.A. (2013). Economic and Disease Burden of Dengue in Southeast Asia. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002055"},{"key":"ref_17","unstructured":"WHO (2024, November 18). Dengue Increase Likely During Rainy Season: WHO Warns. Available online: https:\/\/www.who.int\/westernpacific\/news\/item\/11-06-2019-dengue-increase-likely-during-rainy-season-who-warns."},{"key":"ref_18","first-page":"219","article-title":"Epidemiological Situation of Dengue Infection in Lao P. D. R","volume":"35","author":"Fununaga","year":"1993","journal-title":"Trop. Med."},{"key":"ref_19","first-page":"7","article-title":"National Dengue Surveillance in the Lao People\u2019s Democratic Republic, 2006\u20132012: Epidemiological and Laboratory Findings","volume":"5","author":"Khampapongpane","year":"2014","journal-title":"West. Pac. Surveill. Response J. WPSAR"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"21327","DOI":"10.1038\/s41598-024-71807-3","article-title":"Bayesian Spatio-Temporal Analysis of Dengue Transmission in Lao PDR","volume":"14","author":"Soukavong","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_21","unstructured":"WHO (2009). Dengue Guidelines for Diagnosis, Treatment, Prevention and Control: New Edition, World Health Organization."},{"key":"ref_22","first-page":"1","article-title":"Epidemiology of Dengue Reported in the World Health Organization\u2019s Western Pacific Region, 2013\u20132019","volume":"14","author":"Togami","year":"2023","journal-title":"West. Pac. Surveill. Response J. WPSAR"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Castonguay-Vanier, J., Klitting, R., Sengvilaipaseuth, O., Piorkowski, G., Baronti, C., Sibounheuang, B., Vongsouvath, M., Chanthongthip, A., Thongpaseuth, S., and Mayxay, M. (2018). Molecular Epidemiology of Dengue Viruses in Three Provinces of Lao PDR, 2006\u20132010. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006203"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Bali\u00e8re, C., Calvez, E., Thiberge, J.-M., Somlor, S., Vandenbogaert, M., Grandadam, M., and Caro, V. (2023). A Six Years (2010\u20132016) Longitudinal Survey of the Four Serotypes of Dengue Viruses in Lao PDR. Microorganisms, 11.","DOI":"10.3390\/microorganisms11020243"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Calvez, E., Somlor, S., Viengphouthong, S., Bali\u00e8re, C., Bounmany, P., Keosenhom, S., Caro, V., and Grandadam, M. (2020). Rapid Genotyping Protocol to Improve Dengue Virus Serotype 2 Survey in Lao PDR. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0237384"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Calvez, E., Pommelet, V., Somlor, S., Pompon, J., Viengphouthong, S., Bounmany, P., Chindavong, T.A., Xaybounsou, T., Prasayasith, P., and Keosenhom, S. (2020). Trends of the Dengue Serotype-4 Circulation with Epidemiological, Phylogenetic, and Entomological Insights in Lao PDR between 2015 and 2019. Pathogens, 9.","DOI":"10.3390\/pathogens9090728"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Calvez, E., Bounmany, P., Bali\u00e8re, C., Somlor, S., Viengphouthong, S., Xaybounsou, T., Keosenhom, S., Fangkham, K., Brey, P.T., and Caro, V. (2021). Using Background Sequencing Data to Anticipate DENV-1 Circulation in the Lao PDR. Microorganisms, 9.","DOI":"10.3390\/microorganisms9112263"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Lao, M., Caro, V., Thiberge, J.-M., Bounmany, P., Vongpayloth, K., Buchy, P., Duong, V., Vanhlasy, C., Hospied, J.-M., and Thongsna, M. (2014). Co-Circulation of Dengue Virus Type 3 Genotypes in Vientiane Capital, Lao PDR. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0115569"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1002\/jmv.2176","article-title":"Single Rapid TaqMan Fluorogenic Probe Based PCR Assay That Detects All Four Dengue Serotypes","volume":"66","author":"Warrilow","year":"2002","journal-title":"J. Med. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"113862","DOI":"10.1016\/j.jviromet.2020.113862","article-title":"Improved Detection of Dengue and Zika Viruses Using Multiplex RT-qPCR Assays","volume":"282","author":"Ou","year":"2020","journal-title":"J. Virol. Methods"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1093\/bioinformatics\/bts091","article-title":"UGENE team Unipro UGENE: A Unified Bioinformatics Toolkit","volume":"28","author":"Okonechnikov","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"W232","DOI":"10.1093\/nar\/gkw256","article-title":"W-IQ-TREE: A Fast Online Phylogenetic Tool for Maximum Likelihood Analysis","volume":"44","author":"Trifinopoulos","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"ModelFinder: Fast Model Selection for Accurate Phylogenetic Estimates","volume":"14","author":"Kalyaanamoorthy","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1093\/molbev\/mst024","article-title":"Ultrafast Approximation for Phylogenetic Bootstrap","volume":"30","author":"Minh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Filip, R., Gheorghita Puscaselu, R., Anchidin-Norocel, L., Dimian, M., and Savage, W.K. (2022). Global Challenges to Public Health Care Systems during the COVID-19 Pandemic: A Review of Pandemic Measures and Problems. J. Pers. Med., 12.","DOI":"10.3390\/jpm12081295"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"S40","DOI":"10.3855\/jidc.18687","article-title":"Impact of COVID-19 on Dengue: A Twofold Challenge to Public Health in Sabah, Malaysia","volume":"18","author":"Balakrishnan","year":"2024","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Tangsathapornpong, A., and Thisyakorn, U. (2023). Dengue amid COVID-19 Pandemic. PLoS Glob. Public Health, 3.","DOI":"10.1371\/journal.pgph.0001558"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e2161","DOI":"10.1002\/rmv.2161","article-title":"Covid-19 and Dengue: Double Punches for Dengue-endemic Countries in Asia","volume":"31","author":"Harapan","year":"2021","journal-title":"Rev. Med. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"540","DOI":"10.3390\/idr13020050","article-title":"Potential Misdiagnosis between COVID-19 and Dengue Infection Using Rapid Serological Test","volume":"13","author":"Khairunisa","year":"2021","journal-title":"Infect. Dis. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.3201\/eid2808.220550","article-title":"Emergence of Dengue Virus Serotype 2 Cosmopolitan Genotype, Brazil","volume":"28","author":"Giovanetti","year":"2022","journal-title":"Emerg. Infect. Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2400123","DOI":"10.2807\/1560-7917.ES.2024.29.13.2400123","article-title":"Molecular Epidemiology Identifies the Expansion of the DENV2 Epidemic Lineage from the French Caribbean Islands to French Guiana and Mainland France, 2023 to 2024","volume":"29","author":"Klitting","year":"2024","journal-title":"Euro. Surveill."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"189","DOI":"10.3201\/eid3001.230972","article-title":"Emergence of Dengue Virus Serotype 2 Cosmopolitan Genotype, Colombia","volume":"30","author":"Montilla","year":"2024","journal-title":"Emerg. Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Soo, K.-M., Khalid, B., Ching, S.-M., and Chee, H.-Y. (2016). Meta-Analysis of Dengue Severity during Infection by Different Dengue Virus Serotypes in Primary and Secondary Infections. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0154760"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1038\/s41467-019-09193-y","article-title":"Effects of Infection History on Dengue Virus Infection and Pathogenicity","volume":"10","author":"Tsang","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1093\/aje\/kwf006","article-title":"Spatial and Temporal Circulation of Dengue Virus Serotypes: A Prospective Study of Primary School Children in Kamphaeng Phet, Thailand","volume":"156","author":"Endy","year":"2002","journal-title":"Am. J. Epidemiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"172","DOI":"10.4269\/ajtmh.1988.38.172","article-title":"A Prospective Study of Dengue Infections in Bangkok","volume":"38","author":"Burke","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.4269\/ajtmh.2006.75.1113","article-title":"Dengue Hemorrhagic Fever Caused by Sequential Dengue 1\u20133 Virus Infections over a Long Time Interval: Havana Epidemic, 2001\u20132002","volume":"75","author":"Alvarez","year":"2006","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1093\/aje\/152.9.793","article-title":"Epidemiologic Studies on Dengue in Santiago de Cuba, 1997","volume":"152","author":"Kouri","year":"2000","journal-title":"Am. J. Epidemiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e2318704121","DOI":"10.1073\/pnas.2318704121","article-title":"Climate, Demography, Immunology, and Virology Combine to Drive Two Decades of Dengue Virus Dynamics in Cambodia","volume":"121","author":"Brook","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"605","DOI":"10.2471\/BLT.23.289713","article-title":"National Dengue Surveillance, Cambodia 2002\u20132020","volume":"101","author":"Yek","year":"2023","journal-title":"Bull. World Health Organ."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Phadungsombat, J., Vu, H.T.T., Nguyen, Q.T., Nguyen, H.T.V., Nguyen, H.T.N., Dang, B.T., Nakayama, E.E., Ishizaki, A., Ichimura, H., and Shioda, T. (2023). Molecular Characterization of Dengue Virus Strains from the 2019-2020 Epidemic in Hanoi, Vietnam. Microorganisms, 11.","DOI":"10.3390\/microorganisms11051267"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.3201\/eid2910.230529","article-title":"Reemergence of Cosmopolitan Genotype Dengue Virus Serotype 2, Southern Vietnam","volume":"29","author":"Tran","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Pham, H.T., Pham, T.N.T., Tran, N.H.T., Ha, Q.D., Tran, D.K., Nguyen, N.H.D., Pham, V.H., and Pham, S.T. (2024). Dengue Hemorrhagic Fever in Quang Nam Province (Vietnam) from 2020 to 2022\u2014A Study on Serotypes Distribution and Immunology Factors. Diagnostics, 14.","DOI":"10.3390\/diagnostics14161772"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1126\/science.aac5017","article-title":"Dengue Viruses Cluster Antigenically but Not as Discrete Serotypes","volume":"349","author":"Katzelnick","year":"2015","journal-title":"Science"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1126\/science.abk0058","article-title":"Antigenic Evolution of Dengue Viruses over 20 Years","volume":"374","author":"Katzelnick","year":"2021","journal-title":"Science"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"eadk3259","DOI":"10.1126\/scitranslmed.adk3259","article-title":"Antigenic Distance between Primary and Secondary Dengue Infections Correlates with Disease Risk","volume":"16","author":"Wang","year":"2024","journal-title":"Sci. Transl. Med."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Jagtap, S., Pattabiraman, C., Sankaradoss, A., Krishna, S., and Roy, R. (2023). Evolutionary Dynamics of Dengue Virus in India. PLoS Pathog., 19.","DOI":"10.1371\/journal.ppat.1010862"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"109951","DOI":"10.1016\/j.virol.2023.109951","article-title":"Preexisting Inter-Serotype Immunity Drives Antigenic Evolution of Dengue Virus Serotype 2","volume":"590","author":"Marano","year":"2024","journal-title":"Virology"}],"container-title":["Microorganisms"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/2\/318\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:25:36Z","timestamp":1760027136000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-2607\/13\/2\/318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,1]]},"references-count":59,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["microorganisms13020318"],"URL":"https:\/\/doi.org\/10.3390\/microorganisms13020318","relation":{},"ISSN":["2076-2607"],"issn-type":[{"value":"2076-2607","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,1]]}}}