{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:39:50Z","timestamp":1792100390237,"version":"4.4.0"},"reference-count":45,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shandong Provincial Key Research and Development Program, China","award":["2021CXGC011201"],"award-info":[{"award-number":["2021CXGC011201"]}]},{"name":"Shandong Provincial Key Research and Development Program, China","award":["2023TZXD003"],"award-info":[{"award-number":["2023TZXD003"]}]},{"name":"Shandong Provincial Key Research and Development Program, China","award":["ZR2021MD126"],"award-info":[{"award-number":["ZR2021MD126"]}]},{"name":"Shandong Provincial Key Research and Development Program, China","award":["2015"],"award-info":[{"award-number":["2015"]}]},{"name":"Rural Revitalization Project to Boost Science, Technology and Innovation","award":["2021CXGC011201"],"award-info":[{"award-number":["2021CXGC011201"]}]},{"name":"Rural Revitalization Project to Boost Science, Technology and Innovation","award":["2023TZXD003"],"award-info":[{"award-number":["2023TZXD003"]}]},{"name":"Rural Revitalization Project to Boost Science, Technology and Innovation","award":["ZR2021MD126"],"award-info":[{"award-number":["ZR2021MD126"]}]},{"name":"Rural Revitalization Project to Boost Science, Technology and Innovation","award":["2015"],"award-info":[{"award-number":["2015"]}]},{"name":"Shandong Provincial Natural Science Foundation, China","award":["2021CXGC011201"],"award-info":[{"award-number":["2021CXGC011201"]}]},{"name":"Shandong Provincial Natural Science Foundation, China","award":["2023TZXD003"],"award-info":[{"award-number":["2023TZXD003"]}]},{"name":"Shandong Provincial Natural Science Foundation, China","award":["ZR2021MD126"],"award-info":[{"award-number":["ZR2021MD126"]}]},{"name":"Shandong Provincial Natural Science Foundation, China","award":["2015"],"award-info":[{"award-number":["2015"]}]},{"name":"Taishan Scholars Program of Shandong Province","award":["2021CXGC011201"],"award-info":[{"award-number":["2021CXGC011201"]}]},{"name":"Taishan Scholars Program of Shandong Province","award":["2023TZXD003"],"award-info":[{"award-number":["2023TZXD003"]}]},{"name":"Taishan Scholars Program of Shandong Province","award":["ZR2021MD126"],"award-info":[{"award-number":["ZR2021MD126"]}]},{"name":"Taishan Scholars Program of Shandong Province","award":["2015"],"award-info":[{"award-number":["2015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microorganisms"],"abstract":"<jats:p>Livestock wastewater reuse could be a potential source for the distribution of antibiotics, antibiotic resistance bacteria (ARB), and antibiotic resistance genes (ARGs) in agricultural soil. In this study, soil samples were collected from different depths (0\u201360 cm) of farmland that has been subjected to long-term application of livestock wastewater. The vertical distribution of antibiotics, bacterial communities, and ARGs were assessed to identify the driving factors that could potentially influence the distribution of ARB and ARGs. The results demonstrated distinguished distributions of antibiotics along the soil depths, with tetracyclines (TCs) mainly found in the top 10 cm of the soil (0.11\u20130.31 \u03bcg\/kg), while quinolones (QNs), sulfonamides (SAs), and macrolides (MLs) were detected in all 60 cm of soil depth (0.01\u20130.22 \u03bcg\/kg). The selection pressure of antibiotics to microorganisms led to the proliferation of ARB, especially tetracycline-resistant bacteria and erythromycin-resistant bacteria. In terms of the distribution\/abundance of ARGs, novA and tetA (58) were relatively higher in 0\u201310 cm surface soil, while vanRM and vanRF were mainly detected in the deeper soil. Different ARGs may have the same host bacteria, which lead to the emergence of multidrug resistant bacteria, such as Ilumatobacter sp., Aggregatilinea sp., Rhabdothermincola sp., and Ornithinimicrobium sp. Soil pH, electrical conductivity (EC), and moisture content (MC) could affect the distribution and proliferation of ARB and were found negatively correlated with most of the ARGs except macB. Therefore, it is potentially possible to eliminate\/inhibit the spread of ARGs by adjusting these soil parameters. These findings provide insights into the distribution and dissemination of antibiotics, ARB, and ARGs in agricultural practices of livestock wastewater irrigation and provide effective mitigation strategies to ensure the safe use of livestock wastewater in agriculture.<\/jats:p>","DOI":"10.3390\/microorganisms13030610","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T11:11:27Z","timestamp":1741259487000},"page":"610","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Vertical Distribution and Drivers of Antibiotic Resistance Genes in Agricultural Soil Irrigated with Livestock Wastewater"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4718-2402","authenticated-orcid":false,"given":"Ming","family":"Shang","sequence":"first","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4732-4112","authenticated-orcid":false,"given":"Yongchao","family":"Gao","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Ji","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Du","sequence":"additional","affiliation":[{"name":"WSP Australia Pty Limited, Level 3, Mia Yellagonga Tower 2, 5 Spring Street, Perth, WA 6000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Kong","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Resources and Environment, University of Jinan, Jinan 250022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Shi","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailun","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3553-2125","authenticated-orcid":false,"given":"Xiaodong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Geography & Spatial Information Technology, Ningbo University, Ningbo 315211, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyu","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.envint.2017.10.016","article-title":"Review of antibiotic resistance in China and its environment","volume":"110","author":"Qiao","year":"2018","journal-title":"Environ. 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